Acidianus spindle-shaped virus 1

Taxonomy: Viruses; Fuselloviridae; Betafusellovirus

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D1GF87|D1GF87_9VIRU Uncharacterized protein OS=Acidianus spindle-shaped virus 1 OX=693629 PE=4 SV=1
MM1 pKa = 7.22MSALGDD7 pKa = 3.49VIYY10 pKa = 10.44ILGILIPLLGLIVRR24 pKa = 11.84NYY26 pKa = 10.8LVDD29 pKa = 4.03LLGFIMGTMGFLVFVQNYY47 pKa = 8.09TDD49 pKa = 3.27ITFSASNFYY58 pKa = 10.7LALLPLAFGLMDD70 pKa = 3.9FAFFFNWVRR79 pKa = 11.84EE80 pKa = 3.99EE81 pKa = 4.28RR82 pKa = 11.84II83 pKa = 3.48

Molecular weight: 9.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D1GF86|D1GF86_9VIRU Putative membrane protein OS=Acidianus spindle-shaped virus 1 OX=693629 PE=4 SV=1
MM1 pKa = 7.89GYY3 pKa = 9.51RR4 pKa = 11.84HH5 pKa = 6.24HH6 pKa = 6.66QHH8 pKa = 6.75DD9 pKa = 3.58RR10 pKa = 11.84WIQRR14 pKa = 11.84AVKK17 pKa = 10.19RR18 pKa = 11.84KK19 pKa = 8.93GRR21 pKa = 11.84SHH23 pKa = 7.21RR24 pKa = 11.84YY25 pKa = 9.5LEE27 pKa = 3.92RR28 pKa = 11.84LYY30 pKa = 11.15GKK32 pKa = 10.23RR33 pKa = 11.84AFTKK37 pKa = 10.72DD38 pKa = 2.72GDD40 pKa = 3.97IKK42 pKa = 11.27VEE44 pKa = 4.28YY45 pKa = 8.75IDD47 pKa = 3.66KK48 pKa = 10.79AIRR51 pKa = 11.84HH52 pKa = 5.05VKK54 pKa = 9.97RR55 pKa = 11.84NYY57 pKa = 10.37SKK59 pKa = 10.85GSEE62 pKa = 4.04EE63 pKa = 4.1RR64 pKa = 11.84RR65 pKa = 11.84SLLSALYY72 pKa = 9.64LAKK75 pKa = 10.38RR76 pKa = 11.84LKK78 pKa = 10.21RR79 pKa = 11.84LKK81 pKa = 10.48KK82 pKa = 10.29

Molecular weight: 10.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

38

0

38

7894

58

1231

207.7

23.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.359 ± 0.338

0.785 ± 0.243

4.092 ± 0.391

5.815 ± 0.747

5.257 ± 0.381

6.549 ± 0.622

1.545 ± 0.294

8.044 ± 0.385

6.423 ± 1.179

9.539 ± 0.614

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.28 ± 0.199

5.84 ± 0.645

4.092 ± 0.505

3.192 ± 0.252

3.496 ± 0.682

6.815 ± 0.46

6.575 ± 0.571

6.866 ± 0.341

1.761 ± 0.208

5.675 ± 0.613
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski