Amino acid dipepetide frequency for Rhizobium sp. Leaf371

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.709AlaAla: 16.709 ± 0.161	0.95AlaCys: 0.95 ± 0.029	7.228AlaAsp: 7.228 ± 0.084	7.31AlaGlu: 7.31 ± 0.081	4.81AlaPhe: 4.81 ± 0.064	10.644AlaGly: 10.644 ± 0.091	2.081AlaHis: 2.081 ± 0.047	6.964AlaIle: 6.964 ± 0.077	4.111AlaLys: 4.111 ± 0.062	13.426AlaLeu: 13.426 ± 0.146	3.572AlaMet: 3.572 ± 0.051	2.865AlaAsn: 2.865 ± 0.054	5.149AlaPro: 5.149 ± 0.079	3.585AlaGln: 3.585 ± 0.061	8.498AlaArg: 8.498 ± 0.089	6.856AlaSer: 6.856 ± 0.085	6.441AlaThr: 6.441 ± 0.083	8.814AlaVal: 8.814 ± 0.093	1.378AlaTrp: 1.378 ± 0.03	2.586AlaTyr: 2.586 ± 0.047	0.004AlaXaa: 0.004 ± 0.002
Cys	0.796CysAla: 0.796 ± 0.022	0.084CysCys: 0.084 ± 0.008	0.507CysAsp: 0.507 ± 0.02	0.415CysGlu: 0.415 ± 0.017	0.29CysPhe: 0.29 ± 0.014	0.823CysGly: 0.823 ± 0.029	0.209CysHis: 0.209 ± 0.013	0.395CysIle: 0.395 ± 0.017	0.172CysLys: 0.172 ± 0.011	0.797CysLeu: 0.797 ± 0.023	0.151CysMet: 0.151 ± 0.011	0.179CysAsn: 0.179 ± 0.012	0.358CysPro: 0.358 ± 0.016	0.2CysGln: 0.2 ± 0.013	0.535CysArg: 0.535 ± 0.021	0.373CysSer: 0.373 ± 0.015	0.375CysThr: 0.375 ± 0.017	0.547CysVal: 0.547 ± 0.021	0.097CysTrp: 0.097 ± 0.009	0.16CysTyr: 0.16 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	7.359AspAla: 7.359 ± 0.068	0.47AspCys: 0.47 ± 0.017	3.207AspAsp: 3.207 ± 0.049	3.289AspGlu: 3.289 ± 0.059	2.282AspPhe: 2.282 ± 0.043	5.4AspGly: 5.4 ± 0.079	1.331AspHis: 1.331 ± 0.035	3.679AspIle: 3.679 ± 0.053	1.685AspLys: 1.685 ± 0.042	6.302AspLeu: 6.302 ± 0.079	1.449AspMet: 1.449 ± 0.035	1.373AspAsn: 1.373 ± 0.032	3.436AspPro: 3.436 ± 0.05	1.635AspGln: 1.635 ± 0.034	4.592AspArg: 4.592 ± 0.063	2.115AspSer: 2.115 ± 0.041	2.897AspThr: 2.897 ± 0.053	4.471AspVal: 4.471 ± 0.057	0.922AspTrp: 0.922 ± 0.028	1.478AspTyr: 1.478 ± 0.036	0.001AspXaa: 0.001 ± 0.001
Glu	7.532GluAla: 7.532 ± 0.088	0.283GluCys: 0.283 ± 0.013	2.868GluAsp: 2.868 ± 0.049	3.049GluGlu: 3.049 ± 0.066	1.563GluPhe: 1.563 ± 0.033	4.056GluGly: 4.056 ± 0.059	1.097GluHis: 1.097 ± 0.028	3.797GluIle: 3.797 ± 0.057	2.421GluLys: 2.421 ± 0.052	4.487GluLeu: 4.487 ± 0.061	1.488GluMet: 1.488 ± 0.031	1.72GluAsn: 1.72 ± 0.033	2.603GluPro: 2.603 ± 0.062	1.942GluGln: 1.942 ± 0.039	4.72GluArg: 4.72 ± 0.073	2.321GluSer: 2.321 ± 0.036	4.364GluThr: 4.364 ± 0.062	3.419GluVal: 3.419 ± 0.057	0.571GluTrp: 0.571 ± 0.023	0.88GluTyr: 0.88 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	4.575PheAla: 4.575 ± 0.06	0.378PheCys: 0.378 ± 0.016	2.762PheAsp: 2.762 ± 0.047	2.121PheGlu: 2.121 ± 0.036	1.52PhePhe: 1.52 ± 0.039	3.681PheGly: 3.681 ± 0.057	0.756PheHis: 0.756 ± 0.024	1.823PheIle: 1.823 ± 0.04	1.068PheLys: 1.068 ± 0.033	3.612PheLeu: 3.612 ± 0.062	0.82PheMet: 0.82 ± 0.024	1.11PheAsn: 1.11 ± 0.029	1.67PhePro: 1.67 ± 0.035	1.113PheGln: 1.113 ± 0.033	2.378PheArg: 2.378 ± 0.047	2.522PheSer: 2.522 ± 0.051	2.002PheThr: 2.002 ± 0.039	2.924PheVal: 2.924 ± 0.061	0.513PheTrp: 0.513 ± 0.02	0.914PheTyr: 0.914 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	8.982GlyAla: 8.982 ± 0.091	0.744GlyCys: 0.744 ± 0.024	4.521GlyAsp: 4.521 ± 0.063	4.56GlyGlu: 4.56 ± 0.068	3.743GlyPhe: 3.743 ± 0.05	6.863GlyGly: 6.863 ± 0.101	1.851GlyHis: 1.851 ± 0.038	5.038GlyIle: 5.038 ± 0.07	3.329GlyLys: 3.329 ± 0.06	8.97GlyLeu: 8.97 ± 0.098	2.275GlyMet: 2.275 ± 0.043	2.271GlyAsn: 2.271 ± 0.05	3.174GlyPro: 3.174 ± 0.05	2.789GlyGln: 2.789 ± 0.043	6.121GlyArg: 6.121 ± 0.073	4.711GlySer: 4.711 ± 0.067	4.99GlyThr: 4.99 ± 0.067	5.881GlyVal: 5.881 ± 0.072	1.174GlyTrp: 1.174 ± 0.029	2.207GlyTyr: 2.207 ± 0.044	0.002GlyXaa: 0.002 ± 0.001
His	2.336HisAla: 2.336 ± 0.041	0.189HisCys: 0.189 ± 0.011	1.295HisAsp: 1.295 ± 0.036	0.992HisGlu: 0.992 ± 0.03	0.858HisPhe: 0.858 ± 0.024	1.863HisGly: 1.863 ± 0.043	0.544HisHis: 0.544 ± 0.022	1.024HisIle: 1.024 ± 0.029	0.445HisLys: 0.445 ± 0.021	2.081HisLeu: 2.081 ± 0.039	0.514HisMet: 0.514 ± 0.021	0.383HisAsn: 0.383 ± 0.018	1.304HisPro: 1.304 ± 0.029	0.577HisGln: 0.577 ± 0.021	1.407HisArg: 1.407 ± 0.029	0.86HisSer: 0.86 ± 0.024	0.782HisThr: 0.782 ± 0.027	1.533HisVal: 1.533 ± 0.035	0.274HisTrp: 0.274 ± 0.012	0.502HisTyr: 0.502 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	8.052IleAla: 8.052 ± 0.077	0.512IleCys: 0.512 ± 0.021	4.18IleAsp: 4.18 ± 0.057	3.659IleGlu: 3.659 ± 0.057	1.886IlePhe: 1.886 ± 0.043	5.482IleGly: 5.482 ± 0.074	0.941IleHis: 0.941 ± 0.024	2.51IleIle: 2.51 ± 0.053	1.523IleLys: 1.523 ± 0.035	5.055IleLeu: 5.055 ± 0.067	1.055IleMet: 1.055 ± 0.028	1.486IleAsn: 1.486 ± 0.033	2.389IlePro: 2.389 ± 0.046	1.215IleGln: 1.215 ± 0.033	3.467IleArg: 3.467 ± 0.065	3.124IleSer: 3.124 ± 0.053	2.852IleThr: 2.852 ± 0.049	4.76IleVal: 4.76 ± 0.065	0.592IleTrp: 0.592 ± 0.022	1.131IleTyr: 1.131 ± 0.029	0.001IleXaa: 0.001 ± 0.001
Lys	4.699LysAla: 4.699 ± 0.063	0.13LysCys: 0.13 ± 0.011	1.922LysAsp: 1.922 ± 0.041	1.497LysGlu: 1.497 ± 0.036	0.831LysPhe: 0.831 ± 0.029	2.805LysGly: 2.805 ± 0.058	0.577LysHis: 0.577 ± 0.02	1.901LysIle: 1.901 ± 0.044	1.206LysLys: 1.206 ± 0.033	3.178LysLeu: 3.178 ± 0.059	0.806LysMet: 0.806 ± 0.028	0.859LysAsn: 0.859 ± 0.026	2.105LysPro: 2.105 ± 0.039	0.961LysGln: 0.961 ± 0.029	2.399LysArg: 2.399 ± 0.045	1.868LysSer: 1.868 ± 0.037	2.406LysThr: 2.406 ± 0.047	2.462LysVal: 2.462 ± 0.051	0.312LysTrp: 0.312 ± 0.016	0.57LysTyr: 0.57 ± 0.021	0.001LysXaa: 0.001 ± 0.001
Leu	12.914LeuAla: 12.914 ± 0.137	0.861LeuCys: 0.861 ± 0.027	6.218LeuAsp: 6.218 ± 0.069	5.124LeuGlu: 5.124 ± 0.067	3.777LeuPhe: 3.777 ± 0.055	7.975LeuGly: 7.975 ± 0.081	1.838LeuHis: 1.838 ± 0.04	5.032LeuIle: 5.032 ± 0.073	3.873LeuLys: 3.873 ± 0.055	9.266LeuLeu: 9.266 ± 0.121	2.442LeuMet: 2.442 ± 0.044	2.525LeuAsn: 2.525 ± 0.045	5.365LeuPro: 5.365 ± 0.075	2.885LeuGln: 2.885 ± 0.042	6.328LeuArg: 6.328 ± 0.074	7.439LeuSer: 7.439 ± 0.103	5.81LeuThr: 5.81 ± 0.069	7.66LeuVal: 7.66 ± 0.101	1.034LeuTrp: 1.034 ± 0.027	2.1LeuTyr: 2.1 ± 0.042	0.001LeuXaa: 0.001 ± 0.001
Met	3.15MetAla: 3.15 ± 0.043	0.133MetCys: 0.133 ± 0.009	1.223MetAsp: 1.223 ± 0.029	1.143MetGlu: 1.143 ± 0.031	0.726MetPhe: 0.726 ± 0.02	1.728MetGly: 1.728 ± 0.034	0.382MetHis: 0.382 ± 0.018	1.584MetIle: 1.584 ± 0.032	1.016MetLys: 1.016 ± 0.028	2.47MetLeu: 2.47 ± 0.045	0.713MetMet: 0.713 ± 0.025	0.804MetAsn: 0.804 ± 0.023	1.516MetPro: 1.516 ± 0.032	0.801MetGln: 0.801 ± 0.024	1.834MetArg: 1.834 ± 0.035	1.728MetSer: 1.728 ± 0.034	2.178MetThr: 2.178 ± 0.035	1.689MetVal: 1.689 ± 0.032	0.184MetTrp: 0.184 ± 0.011	0.275MetTyr: 0.275 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.354AsnAla: 3.354 ± 0.061	0.2AsnCys: 0.2 ± 0.012	1.513AsnAsp: 1.513 ± 0.038	1.292AsnGlu: 1.292 ± 0.029	0.933AsnPhe: 0.933 ± 0.024	2.509AsnGly: 2.509 ± 0.05	0.488AsnHis: 0.488 ± 0.019	1.435AsnIle: 1.435 ± 0.032	0.678AsnLys: 0.678 ± 0.025	2.537AsnLeu: 2.537 ± 0.04	0.653AsnMet: 0.653 ± 0.019	0.665AsnAsn: 0.665 ± 0.025	1.765AsnPro: 1.765 ± 0.033	0.746AsnGln: 0.746 ± 0.024	1.868AsnArg: 1.868 ± 0.036	1.169AsnSer: 1.169 ± 0.033	1.304AsnThr: 1.304 ± 0.033	1.874AsnVal: 1.874 ± 0.038	0.404AsnTrp: 0.404 ± 0.019	0.609AsnTyr: 0.609 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	6.054ProAla: 6.054 ± 0.076	0.262ProCys: 0.262 ± 0.013	3.75ProAsp: 3.75 ± 0.056	3.47ProGlu: 3.47 ± 0.053	2.04ProPhe: 2.04 ± 0.038	4.16ProGly: 4.16 ± 0.057	1.037ProHis: 1.037 ± 0.03	2.355ProIle: 2.355 ± 0.048	1.675ProLys: 1.675 ± 0.039	4.642ProLeu: 4.642 ± 0.068	1.163ProMet: 1.163 ± 0.029	1.203ProAsn: 1.203 ± 0.029	2.226ProPro: 2.226 ± 0.051	1.524ProGln: 1.524 ± 0.031	2.617ProArg: 2.617 ± 0.053	2.787ProSer: 2.787 ± 0.05	2.418ProThr: 2.418 ± 0.047	4.362ProVal: 4.362 ± 0.061	0.58ProTrp: 0.58 ± 0.02	1.166ProTyr: 1.166 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	4.186GlnAla: 4.186 ± 0.067	0.169GlnCys: 0.169 ± 0.01	1.396GlnAsp: 1.396 ± 0.031	1.459GlnGlu: 1.459 ± 0.037	1.018GlnPhe: 1.018 ± 0.028	2.16GlnGly: 2.16 ± 0.04	0.567GlnHis: 0.567 ± 0.021	1.848GlnIle: 1.848 ± 0.053	1.113GlnLys: 1.113 ± 0.029	2.611GlnLeu: 2.611 ± 0.05	0.923GlnMet: 0.923 ± 0.029	0.806GlnAsn: 0.806 ± 0.028	1.701GlnPro: 1.701 ± 0.04	1.194GlnGln: 1.194 ± 0.037	2.251GlnArg: 2.251 ± 0.043	1.941GlnSer: 1.941 ± 0.042	1.934GlnThr: 1.934 ± 0.034	2.054GlnVal: 2.054 ± 0.046	0.331GlnTrp: 0.331 ± 0.017	0.55GlnTyr: 0.55 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	7.455ArgAla: 7.455 ± 0.086	0.421ArgCys: 0.421 ± 0.018	4.178ArgAsp: 4.178 ± 0.062	3.925ArgGlu: 3.925 ± 0.059	2.952ArgPhe: 2.952 ± 0.043	4.494ArgGly: 4.494 ± 0.061	1.769ArgHis: 1.769 ± 0.034	4.205ArgIle: 4.205 ± 0.052	2.351ArgLys: 2.351 ± 0.045	7.673ArgLeu: 7.673 ± 0.092	1.955ArgMet: 1.955 ± 0.043	1.877ArgAsn: 1.877 ± 0.037	3.341ArgPro: 3.341 ± 0.054	2.597ArgGln: 2.597 ± 0.043	5.338ArgArg: 5.338 ± 0.08	3.771ArgSer: 3.771 ± 0.047	3.667ArgThr: 3.667 ± 0.056	4.392ArgVal: 4.392 ± 0.055	0.891ArgTrp: 0.891 ± 0.028	1.692ArgTyr: 1.692 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	6.527SerAla: 6.527 ± 0.085	0.34SerCys: 0.34 ± 0.017	3.287SerAsp: 3.287 ± 0.058	2.973SerGlu: 2.973 ± 0.049	2.351SerPhe: 2.351 ± 0.045	5.891SerGly: 5.891 ± 0.074	1.106SerHis: 1.106 ± 0.029	3.08SerIle: 3.08 ± 0.049	1.696SerLys: 1.696 ± 0.036	5.857SerLeu: 5.857 ± 0.065	1.403SerMet: 1.403 ± 0.033	1.447SerAsn: 1.447 ± 0.026	2.824SerPro: 2.824 ± 0.046	1.733SerGln: 1.733 ± 0.039	3.733SerArg: 3.733 ± 0.055	3.197SerSer: 3.197 ± 0.064	2.965SerThr: 2.965 ± 0.066	4.29SerVal: 4.29 ± 0.054	0.71SerTrp: 0.71 ± 0.019	1.303SerTyr: 1.303 ± 0.033	0.004SerXaa: 0.004 ± 0.002
Thr	6.832ThrAla: 6.832 ± 0.095	0.402ThrCys: 0.402 ± 0.018	3.219ThrAsp: 3.219 ± 0.053	2.815ThrGlu: 2.815 ± 0.043	2.282ThrPhe: 2.282 ± 0.045	5.417ThrGly: 5.417 ± 0.073	1.073ThrHis: 1.073 ± 0.025	3.403ThrIle: 3.403 ± 0.048	1.64ThrLys: 1.64 ± 0.041	6.356ThrLeu: 6.356 ± 0.082	1.293ThrMet: 1.293 ± 0.03	1.352ThrAsn: 1.352 ± 0.03	3.172ThrPro: 3.172 ± 0.049	1.502ThrGln: 1.502 ± 0.035	3.437ThrArg: 3.437 ± 0.049	3.137ThrSer: 3.137 ± 0.056	3.182ThrThr: 3.182 ± 0.056	4.861ThrVal: 4.861 ± 0.069	0.616ThrTrp: 0.616 ± 0.019	1.314ThrTyr: 1.314 ± 0.034	0.001ThrXaa: 0.001 ± 0.001
Val	8.968ValAla: 8.968 ± 0.097	0.605ValCys: 0.605 ± 0.02	4.017ValAsp: 4.017 ± 0.052	4.436ValGlu: 4.436 ± 0.057	2.987ValPhe: 2.987 ± 0.045	5.392ValGly: 5.392 ± 0.076	1.366ValHis: 1.366 ± 0.033	4.191ValIle: 4.191 ± 0.062	2.393ValLys: 2.393 ± 0.042	7.581ValLeu: 7.581 ± 0.094	1.846ValMet: 1.846 ± 0.042	2.006ValAsn: 2.006 ± 0.037	3.671ValPro: 3.671 ± 0.056	2.021ValGln: 2.021 ± 0.035	4.741ValArg: 4.741 ± 0.058	4.878ValSer: 4.878 ± 0.06	4.839ValThr: 4.839 ± 0.065	5.927ValVal: 5.927 ± 0.079	0.861ValTrp: 0.861 ± 0.025	1.494ValTyr: 1.494 ± 0.038	0.001ValXaa: 0.001 ± 0.001
Trp	1.087TrpAla: 1.087 ± 0.03	0.122TrpCys: 0.122 ± 0.009	0.578TrpAsp: 0.578 ± 0.021	0.521TrpGlu: 0.521 ± 0.02	0.471TrpPhe: 0.471 ± 0.019	0.778TrpGly: 0.778 ± 0.026	0.294TrpHis: 0.294 ± 0.015	0.637TrpIle: 0.637 ± 0.021	0.459TrpLys: 0.459 ± 0.018	1.393TrpLeu: 1.393 ± 0.037	0.331TrpMet: 0.331 ± 0.017	0.457TrpAsn: 0.457 ± 0.016	0.614TrpPro: 0.614 ± 0.019	0.521TrpGln: 0.521 ± 0.018	0.95TrpArg: 0.95 ± 0.029	0.802TrpSer: 0.802 ± 0.024	0.754TrpThr: 0.754 ± 0.023	0.714TrpVal: 0.714 ± 0.023	0.22TrpTrp: 0.22 ± 0.012	0.278TrpTyr: 0.278 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.411TyrAla: 2.411 ± 0.05	0.21TyrCys: 0.21 ± 0.014	1.505TyrAsp: 1.505 ± 0.039	1.128TyrGlu: 1.128 ± 0.028	0.942TyrPhe: 0.942 ± 0.025	2.073TyrGly: 2.073 ± 0.04	0.457TyrHis: 0.457 ± 0.018	0.97TyrIle: 0.97 ± 0.027	0.64TyrLys: 0.64 ± 0.022	2.173TyrLeu: 2.173 ± 0.041	0.424TyrMet: 0.424 ± 0.016	0.608TyrAsn: 0.608 ± 0.022	1.111TyrPro: 1.111 ± 0.03	0.671TyrGln: 0.671 ± 0.023	1.728TyrArg: 1.728 ± 0.035	1.111TyrSer: 1.111 ± 0.025	1.146TyrThr: 1.146 ± 0.028	1.588TyrVal: 1.588 ± 0.037	0.315TyrTrp: 0.315 ± 0.015	0.574TyrTyr: 0.574 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.002XaaGlu: 0.002 ± 0.002	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.004XaaArg: 0.004 ± 0.002	0.003XaaSer: 0.003 ± 0.002	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.001XaaTyr: 0.001 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4411 proteins (1421238 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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