Sphingomonas koreensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria;

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4485 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L6JFB7|A0A1L6JFB7_9SPHN DUF4880 domain-containing protein OS=Sphingomonas koreensis OX=93064 GN=BDW16_3042 PE=4 SV=1
MM1 pKa = 7.79TDD3 pKa = 3.61LDD5 pKa = 4.22RR6 pKa = 11.84DD7 pKa = 3.58IATDD11 pKa = 3.35EE12 pKa = 4.16TDD14 pKa = 5.0RR15 pKa = 11.84LIASNKK21 pKa = 9.37VEE23 pKa = 4.1GTSVFDD29 pKa = 3.82AQGEE33 pKa = 4.36KK34 pKa = 10.71LGTIYY39 pKa = 11.17NFMVNKK45 pKa = 10.46DD46 pKa = 3.62SGQVEE51 pKa = 4.46YY52 pKa = 10.95AVLQFGGLFGLGADD66 pKa = 4.33YY67 pKa = 11.33YY68 pKa = 9.47PLPWEE73 pKa = 4.21KK74 pKa = 10.63LSYY77 pKa = 10.9DD78 pKa = 3.54VDD80 pKa = 3.53EE81 pKa = 5.15GGYY84 pKa = 10.29VVDD87 pKa = 4.04IDD89 pKa = 5.01KK90 pKa = 10.84EE91 pKa = 4.17ALEE94 pKa = 4.38DD95 pKa = 3.54APRR98 pKa = 11.84YY99 pKa = 10.4GDD101 pKa = 3.74DD102 pKa = 3.89TEE104 pKa = 4.18PAYY107 pKa = 10.79DD108 pKa = 3.32RR109 pKa = 11.84AYY111 pKa = 10.14GEE113 pKa = 4.01QVYY116 pKa = 10.15GYY118 pKa = 10.87YY119 pKa = 11.05GLTYY123 pKa = 9.98PAAA126 pKa = 4.64

Molecular weight: 14.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L6JGD6|A0A1L6JGD6_9SPHN Ribonuclease P protein component OS=Sphingomonas koreensis OX=93064 GN=rnpA PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84NKK41 pKa = 10.45LSAA44 pKa = 3.94

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4485

0

4485

1424114

29

6075

317.5

34.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.698 ± 0.054

0.698 ± 0.01

5.811 ± 0.029

5.367 ± 0.044

3.534 ± 0.021

9.081 ± 0.058

1.883 ± 0.018

4.991 ± 0.024

2.919 ± 0.029

9.808 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.324 ± 0.02

2.568 ± 0.039

5.394 ± 0.036

3.024 ± 0.02

7.508 ± 0.052

5.127 ± 0.037

5.405 ± 0.051

7.165 ± 0.029

1.477 ± 0.018

2.218 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski