Pigmentiphaga aceris
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5191 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C0B527|A0A5C0B527_9BURK ABC transporter substrate-binding protein OS=Pigmentiphaga aceris OX=1940612 GN=FXN63_08210 PE=4 SV=1
MM1 pKa = 8.67 SMIYY5 pKa = 9.72 TYY7 pKa = 11.72 ANAPASAADD16 pKa = 3.46 IQAMCEE22 pKa = 4.05 QLGLPATCWLGGFWAAHH39 pKa = 5.7 NGAMLNDD46 pKa = 3.98 LVVIYY51 pKa = 8.76 ATDD54 pKa = 5.1 DD55 pKa = 3.15 IAEE58 pKa = 4.32 RR59 pKa = 11.84 NQTYY63 pKa = 10.67 DD64 pKa = 2.88 VGTSFPDD71 pKa = 3.49 HH72 pKa = 6.85 VSVGDD77 pKa = 3.77 DD78 pKa = 3.08 SGGRR82 pKa = 11.84 LILMPKK88 pKa = 9.55 SGAPRR93 pKa = 11.84 FHH95 pKa = 7.69 LLDD98 pKa = 4.34 AGDD101 pKa = 4.45 PFIEE105 pKa = 4.85 DD106 pKa = 3.3 ADD108 pKa = 4.0 VFDD111 pKa = 4.34 SLEE114 pKa = 3.97 ALVAYY119 pKa = 9.13 VVSEE123 pKa = 4.21 DD124 pKa = 3.26
Molecular weight: 13.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.783
IPC_protein 3.77
Toseland 3.541
ProMoST 3.897
Dawson 3.783
Bjellqvist 3.986
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.452
Solomon 3.757
Lehninger 3.719
Nozaki 3.897
DTASelect 4.177
Thurlkill 3.617
EMBOSS 3.757
Sillero 3.897
Patrickios 0.604
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.817
Protein with the highest isoelectric point:
>tr|A0A5C0B2N7|A0A5C0B2N7_9BURK Dehydratase OS=Pigmentiphaga aceris OX=1940612 GN=FXN63_20615 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.0 RR14 pKa = 11.84 THH16 pKa = 5.76 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 9.39 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.58 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5191
0
5191
1790400
21
2912
344.9
37.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.613 ± 0.044
0.786 ± 0.011
5.58 ± 0.027
4.85 ± 0.032
3.514 ± 0.018
8.179 ± 0.039
2.133 ± 0.017
4.743 ± 0.024
2.973 ± 0.024
10.673 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.436 ± 0.018
2.761 ± 0.022
5.132 ± 0.024
3.841 ± 0.022
6.763 ± 0.038
5.893 ± 0.025
5.59 ± 0.033
7.884 ± 0.029
1.397 ± 0.016
2.259 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here