Gymnodraco acuticeps (Antarctic dragonfish)
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 39919 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6P8TVL0|A0A6P8TVL0_GYMAC malectin OS=Gymnodraco acuticeps OX=8218 GN=mlec PE=3 SV=1
MM1 pKa = 7.7 SYY3 pKa = 10.7 CKK5 pKa = 10.08 QEE7 pKa = 3.98 GKK9 pKa = 10.57 DD10 pKa = 3.37 RR11 pKa = 11.84 IIFVTKK17 pKa = 9.62 EE18 pKa = 3.64 DD19 pKa = 4.02 HH20 pKa = 6.28 EE21 pKa = 4.77 APSNAEE27 pKa = 4.72 LIADD31 pKa = 4.71 DD32 pKa = 5.03 PDD34 pKa = 4.61 DD35 pKa = 4.56 PYY37 pKa = 11.6 EE38 pKa = 4.13 EE39 pKa = 4.29 QGLILANGDD48 pKa = 3.78 INWNCPCLGGMASGPCGSQFKK69 pKa = 10.12 EE70 pKa = 4.0 AFSCFHH76 pKa = 6.08 YY77 pKa = 10.87 SKK79 pKa = 11.23 EE80 pKa = 4.0 EE81 pKa = 3.96 LKK83 pKa = 11.19 GSDD86 pKa = 4.22 CIDD89 pKa = 3.5 NFRR92 pKa = 11.84 GMQEE96 pKa = 4.27 CMQKK100 pKa = 10.35 YY101 pKa = 9.4 PEE103 pKa = 5.35 LYY105 pKa = 9.17 PQEE108 pKa = 4.07 EE109 pKa = 4.55 DD110 pKa = 3.78 KK111 pKa = 11.23 EE112 pKa = 4.58 SSSEE116 pKa = 4.05 AEE118 pKa = 4.33 SNSGSGSAAPTEE130 pKa = 5.16 GSASPPEE137 pKa = 4.42 TDD139 pKa = 4.04 SPPAAATTPASEE151 pKa = 4.4 ATPPAADD158 pKa = 3.76 ATPPAADD165 pKa = 3.64 ATPAADD171 pKa = 4.0 ATPPAADD178 pKa = 4.41 ASPDD182 pKa = 3.63 SASPTEE188 pKa = 4.19 TQSASS193 pKa = 3.1
Molecular weight: 20.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.884
IPC_protein 3.846
Toseland 3.656
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.681
Grimsley 3.567
Solomon 3.808
Lehninger 3.757
Nozaki 3.935
DTASelect 4.101
Thurlkill 3.694
EMBOSS 3.732
Sillero 3.961
Patrickios 1.1
IPC_peptide 3.808
IPC2_peptide 3.948
IPC2.peptide.svr19 3.861
Protein with the highest isoelectric point:
>tr|A0A6P8WKT5|A0A6P8WKT5_GYMAC b(0 +)-type amino acid transporter 1 OS=Gymnodraco acuticeps OX=8218 GN=slc7a9 PE=4 SV=1
MM1 pKa = 7.4 ATRR4 pKa = 11.84 SQPMSHH10 pKa = 6.2 RR11 pKa = 11.84 TPTGRR16 pKa = 11.84 GSSFAIIITAACDD29 pKa = 3.52 QEE31 pKa = 5.69 SSVAPSNTEE40 pKa = 3.54 QRR42 pKa = 11.84 KK43 pKa = 7.85 KK44 pKa = 9.29 TIQCQPADD52 pKa = 3.04 GRR54 pKa = 11.84 TRR56 pKa = 11.84 QKK58 pKa = 10.43 NGSRR62 pKa = 11.84 TRR64 pKa = 11.84 QKK66 pKa = 10.1 NGSRR70 pKa = 11.84 TRR72 pKa = 11.84 QKK74 pKa = 10.1 NGSRR78 pKa = 11.84 TRR80 pKa = 11.84 QKK82 pKa = 10.1 NGSRR86 pKa = 11.84 TRR88 pKa = 11.84 QKK90 pKa = 10.1 NGSRR94 pKa = 11.84 TRR96 pKa = 11.84 QKK98 pKa = 10.1 NGSRR102 pKa = 11.84 TRR104 pKa = 11.84 QKK106 pKa = 10.1 NGSRR110 pKa = 11.84 TRR112 pKa = 11.84 QKK114 pKa = 10.1 NGSRR118 pKa = 11.84 TRR120 pKa = 11.84 QKK122 pKa = 10.1 NGSRR126 pKa = 11.84 TRR128 pKa = 11.84 QKK130 pKa = 10.1 NGSRR134 pKa = 11.84 TRR136 pKa = 11.84 QKK138 pKa = 10.1 NGSRR142 pKa = 11.84 TRR144 pKa = 11.84 QKK146 pKa = 10.1 NGSRR150 pKa = 11.84 TRR152 pKa = 11.84 QKK154 pKa = 10.1 NGSRR158 pKa = 11.84 TRR160 pKa = 11.84 QKK162 pKa = 10.1 NGSRR166 pKa = 11.84 TRR168 pKa = 11.84 QKK170 pKa = 10.1 NGSRR174 pKa = 11.84 TRR176 pKa = 11.84 QKK178 pKa = 10.1 NGSRR182 pKa = 11.84 TRR184 pKa = 11.84 QKK186 pKa = 9.96 TGGTRR191 pKa = 11.84 QKK193 pKa = 10.63 NGRR196 pKa = 11.84 GAQQKK201 pKa = 9.53 NGSRR205 pKa = 11.84 THH207 pKa = 5.92 HH208 pKa = 6.04 RR209 pKa = 11.84 NGRR212 pKa = 11.84 EE213 pKa = 3.82 TKK215 pKa = 10.43 GRR217 pKa = 3.62
Molecular weight: 24.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.423
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.354
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.062
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.098
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
24714
15205
39919
28106559
31
26292
704.1
78.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.527 ± 0.011
2.092 ± 0.01
5.278 ± 0.007
7.209 ± 0.017
3.359 ± 0.007
6.351 ± 0.018
2.628 ± 0.007
4.204 ± 0.009
5.723 ± 0.017
9.26 ± 0.018
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.408 ± 0.005
3.843 ± 0.008
5.956 ± 0.017
4.902 ± 0.013
5.657 ± 0.011
9.078 ± 0.017
5.734 ± 0.012
6.15 ± 0.01
1.069 ± 0.004
2.566 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here