Chlorobium ferrooxidans DSM 13031

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae; Chlorobium/Pelodictyon group; Chlorobium; Chlorobium ferrooxidans

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2158 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0YQE8|Q0YQE8_9CHLB PilT protein-like OS=Chlorobium ferrooxidans DSM 13031 OX=377431 GN=CferDRAFT_0590 PE=4 SV=1
MM1 pKa = 7.57IKK3 pKa = 10.6GNEE6 pKa = 4.1SPMSFIKK13 pKa = 10.64QADD16 pKa = 3.66NVGRR20 pKa = 11.84TGTFTIGTNGVWSYY34 pKa = 9.16TANSAFDD41 pKa = 3.58SLNVRR46 pKa = 11.84EE47 pKa = 4.73SIPEE51 pKa = 3.98RR52 pKa = 11.84FSVTLTDD59 pKa = 3.81GKK61 pKa = 8.22TQVEE65 pKa = 4.33RR66 pKa = 11.84VQITGTNDD74 pKa = 2.95AAVISGDD81 pKa = 3.22VSTTIAEE88 pKa = 3.99TDD90 pKa = 3.33EE91 pKa = 4.99ALTATGQLAVTDD103 pKa = 4.01IDD105 pKa = 3.89NPSLFVAQIEE115 pKa = 4.69TEE117 pKa = 4.41GIHH120 pKa = 6.33GKK122 pKa = 10.41FSIDD126 pKa = 3.86EE127 pKa = 4.37SGLWSYY133 pKa = 10.5IANSAFDD140 pKa = 3.98EE141 pKa = 4.51LNEE144 pKa = 4.5GEE146 pKa = 4.67SLTDD150 pKa = 3.5SFIVTAADD158 pKa = 3.36GTTEE162 pKa = 4.14VVRR165 pKa = 11.84VTITGTNEE173 pKa = 3.25ILPAGGSVAEE183 pKa = 4.23SLPVLSSSGNKK194 pKa = 9.22SVAIITGDD202 pKa = 3.19INASALEE209 pKa = 4.16TNAVITATGNLSVASNPLPFVAEE232 pKa = 4.08NGRR235 pKa = 11.84VGSTGIFSIGADD247 pKa = 3.55GLWNFTANSAFDD259 pKa = 3.49GMNVGEE265 pKa = 4.31TVKK268 pKa = 10.84EE269 pKa = 4.18SFTVSTVDD277 pKa = 3.45GMTEE281 pKa = 4.11VVTVTITGTNDD292 pKa = 2.72VAVISGDD299 pKa = 3.51FNATVLEE306 pKa = 4.41TNALIAVTGRR316 pKa = 11.84LSAVDD321 pKa = 3.57IDD323 pKa = 3.85NPAIFSAQSGIKK335 pKa = 10.19GMYY338 pKa = 9.79GVFDD342 pKa = 4.11MGSDD346 pKa = 4.03GAWSYY351 pKa = 11.25RR352 pKa = 11.84AGSAFDD358 pKa = 3.76SLNIGDD364 pKa = 4.5TLTEE368 pKa = 4.26SFTASTIDD376 pKa = 3.57GTSGLVTVTIAGTNDD391 pKa = 3.05AAVITGDD398 pKa = 3.3SSASYY403 pKa = 11.11VEE405 pKa = 4.5TNAAMTVMGRR415 pKa = 11.84LLVNDD420 pKa = 3.9VDD422 pKa = 3.94NPAAFVAQSDD432 pKa = 4.07SKK434 pKa = 11.43GRR436 pKa = 11.84TGTFSIGTDD445 pKa = 3.92GVWSYY450 pKa = 9.11TANSAFDD457 pKa = 3.62GLNVGGKK464 pKa = 9.78VKK466 pKa = 10.8DD467 pKa = 3.72LFSVFTSDD475 pKa = 2.66GTSQVVSVTITGTNDD490 pKa = 2.81AAIITGDD497 pKa = 3.86LSPTVFEE504 pKa = 4.62TNTAVSLTGRR514 pKa = 11.84LRR516 pKa = 11.84AMDD519 pKa = 4.15MDD521 pKa = 4.06NPSIFVAQNGITGLTGTFSIGMDD544 pKa = 3.68GAWSYY549 pKa = 10.86RR550 pKa = 11.84ANSAFDD556 pKa = 3.43SLKK559 pKa = 10.43VGEE562 pKa = 4.6TVTEE566 pKa = 4.23SFSVSTVDD574 pKa = 3.46GTSEE578 pKa = 4.19LVTVIITGTDD588 pKa = 3.31DD589 pKa = 3.71ASVVTGNFSASVAEE603 pKa = 4.01TDD605 pKa = 4.89APITANGTLTVSDD618 pKa = 3.93IDD620 pKa = 3.91SPFAFVAQSGTKK632 pKa = 8.58GTLGLFSIGIDD643 pKa = 3.72GAWDD647 pKa = 4.11FRR649 pKa = 11.84ASSAFDD655 pKa = 3.53NLNVGDD661 pKa = 4.16TLTEE665 pKa = 4.15SFAVTTIDD673 pKa = 3.51GTSDD677 pKa = 3.3LVTVTITGTNDD688 pKa = 2.9AAVITGSLHH697 pKa = 6.39ASEE700 pKa = 5.51DD701 pKa = 3.62EE702 pKa = 4.04SDD704 pKa = 3.99AMITATGTLHH714 pKa = 6.3ATDD717 pKa = 4.39VDD719 pKa = 4.08SSSAFIAQTDD729 pKa = 3.85VAGTNGTFSIGTDD742 pKa = 3.83GVWIFSANSAFDD754 pKa = 3.61GLNVDD759 pKa = 3.83SSVTEE764 pKa = 4.17SFTVTTADD772 pKa = 3.37GTSEE776 pKa = 4.26VVTVTINGTNDD787 pKa = 3.06AAVITGDD794 pKa = 3.96FSATVAEE801 pKa = 4.39TNEE804 pKa = 3.71ASTATGHH811 pKa = 6.28LTVTDD816 pKa = 4.79LDD818 pKa = 3.79NPAIFLIQTDD828 pKa = 3.43NAGFNGSFSIDD839 pKa = 3.44SDD841 pKa = 4.21GLWSYY846 pKa = 10.72SANSAFDD853 pKa = 3.65YY854 pKa = 11.4LNDD857 pKa = 3.72GDD859 pKa = 4.7SLVEE863 pKa = 4.0SFIVEE868 pKa = 4.44SADD871 pKa = 3.31GTSQIVMLTIMGTNDD886 pKa = 3.06AAVITGDD893 pKa = 3.88FSASVDD899 pKa = 3.63EE900 pKa = 4.57TNVPITATGHH910 pKa = 6.63LSVTDD915 pKa = 3.46VDD917 pKa = 4.12NPALFVVQTDD927 pKa = 3.35TGGINGSFSIDD938 pKa = 3.33AAGLWSYY945 pKa = 10.68AANSAFDD952 pKa = 4.0EE953 pKa = 4.59LNAGDD958 pKa = 4.29TLTEE962 pKa = 4.18SFTVSTMDD970 pKa = 3.02GTTEE974 pKa = 3.8IVTVTITGTNDD985 pKa = 3.11ALTTSQIVTQLTTSWGSGLTGYY1007 pKa = 8.51TFTWPADD1014 pKa = 3.66LTSISYY1020 pKa = 10.32AINEE1024 pKa = 4.23GTPTNVDD1031 pKa = 3.08GYY1033 pKa = 8.71TPSEE1037 pKa = 4.37GGTSLVQMSALQVATARR1054 pKa = 11.84LSFQLLDD1061 pKa = 4.87DD1062 pKa = 4.37IMAKK1066 pKa = 10.29SSGADD1071 pKa = 3.12HH1072 pKa = 7.17RR1073 pKa = 11.84LVQIADD1079 pKa = 3.62PSANITLNYY1088 pKa = 9.95SSNTGGGTYY1097 pKa = 9.7TISFGTVDD1105 pKa = 3.41TVTQSFSITAEE1116 pKa = 4.17QIWLSSAWSSNSDD1129 pKa = 2.43IGMSLGSYY1137 pKa = 10.78GLFTMIHH1144 pKa = 6.58EE1145 pKa = 4.8IGHH1148 pKa = 6.56ALGLSHH1154 pKa = 7.48PGVYY1158 pKa = 10.16DD1159 pKa = 3.71AGNGGTITYY1168 pKa = 10.13QNNAAFAEE1176 pKa = 4.47DD1177 pKa = 3.41NRR1179 pKa = 11.84KK1180 pKa = 7.38YY1181 pKa = 9.59TVMSYY1186 pKa = 10.51FGGYY1190 pKa = 9.51LPGYY1194 pKa = 8.75GWYY1197 pKa = 9.43PDD1199 pKa = 3.39GTYY1202 pKa = 10.92SSFIFPQTPMLYY1214 pKa = 10.32DD1215 pKa = 3.65IAAIQALHH1223 pKa = 6.41GADD1226 pKa = 3.0TVTRR1230 pKa = 11.84TGDD1233 pKa = 3.3TIYY1236 pKa = 11.09GFNCNLASTDD1246 pKa = 3.56AEE1248 pKa = 3.95RR1249 pKa = 11.84PIYY1252 pKa = 10.68DD1253 pKa = 3.62FTSNATPIFTIWDD1266 pKa = 3.69AGGMDD1271 pKa = 4.58TIDD1274 pKa = 3.43CSGYY1278 pKa = 6.87TTQQIINLNPGTYY1291 pKa = 9.97SSLGGMTEE1299 pKa = 3.93NVAIAFDD1306 pKa = 4.03CTIEE1310 pKa = 4.09RR1311 pKa = 11.84AVGGSGNDD1319 pKa = 3.44VLIGSAALNYY1329 pKa = 10.64LNGGNAGDD1337 pKa = 3.99FYY1339 pKa = 10.91IVNRR1343 pKa = 11.84AGDD1346 pKa = 3.55HH1347 pKa = 6.06LAAEE1351 pKa = 4.71FADD1354 pKa = 3.82TGTTGSDD1361 pKa = 3.03EE1362 pKa = 4.07VRR1364 pKa = 11.84FASTIEE1370 pKa = 4.22HH1371 pKa = 6.67DD1372 pKa = 4.05TLTLFAGDD1380 pKa = 3.53TGIEE1384 pKa = 3.85RR1385 pKa = 11.84VVIGTGVGISGVTTGTTSLDD1405 pKa = 3.13VDD1407 pKa = 4.12ASAVLNPIVITGNAGANKK1425 pKa = 8.89LTGTTYY1431 pKa = 11.5NDD1433 pKa = 2.92VLYY1436 pKa = 10.56GGKK1439 pKa = 8.34GTDD1442 pKa = 4.19LLNGGNGSDD1451 pKa = 3.23IYY1453 pKa = 10.85HH1454 pKa = 6.87IFSADD1459 pKa = 3.07EE1460 pKa = 3.96HH1461 pKa = 7.15LSAEE1465 pKa = 4.36FSDD1468 pKa = 3.78TGTTGSDD1475 pKa = 3.11DD1476 pKa = 3.49VRR1478 pKa = 11.84FGSTIANDD1486 pKa = 3.81TLTLYY1491 pKa = 10.9AGDD1494 pKa = 3.73TGIEE1498 pKa = 4.01RR1499 pKa = 11.84VTATGTATLNIDD1511 pKa = 3.37ASGVLNGLLLTGNAGSNTLTGTAYY1535 pKa = 10.93CDD1537 pKa = 3.46TFTGGSGADD1546 pKa = 3.28SFVFTYY1552 pKa = 10.49QLQPVVNKK1560 pKa = 9.12DD1561 pKa = 3.54TIIDD1565 pKa = 3.9FQSGSDD1571 pKa = 3.32VLAFSKK1577 pKa = 11.03GSFTGITSSAGGLLSSAEE1595 pKa = 4.11FWSEE1599 pKa = 3.29AGATVGHH1606 pKa = 7.13DD1607 pKa = 3.17SDD1609 pKa = 5.57DD1610 pKa = 4.69RR1611 pKa = 11.84IIYY1614 pKa = 7.58DD1615 pKa = 3.59TATGNLYY1622 pKa = 10.79YY1623 pKa = 10.61DD1624 pKa = 4.38DD1625 pKa = 5.87DD1626 pKa = 5.74GNGSANARR1634 pKa = 11.84LVALIGTTAHH1644 pKa = 7.04PALLYY1649 pKa = 10.12TDD1651 pKa = 3.98ISIISS1656 pKa = 3.46

Molecular weight: 170.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0YRE3|Q0YRE3_9CHLB Cyclic peptide transporter OS=Chlorobium ferrooxidans DSM 13031 OX=377431 GN=CferDRAFT_0891 PE=4 SV=1
MM1 pKa = 7.45LLSRR5 pKa = 11.84VLPPLQVWKK14 pKa = 9.29TEE16 pKa = 3.7AMKK19 pKa = 10.63QSSTPRR25 pKa = 11.84VCRR28 pKa = 11.84DD29 pKa = 2.78NSFTSKK35 pKa = 10.23QPQKK39 pKa = 10.7ARR41 pKa = 11.84LRR43 pKa = 11.84IVLFLLVIPLLLALSLLFGPSGIGLPDD70 pKa = 3.47MNSPAGGAILSLRR83 pKa = 11.84YY84 pKa = 9.65SRR86 pKa = 11.84LLMGMMTGAALSASGVVFQALLRR109 pKa = 11.84NPLAEE114 pKa = 4.37PYY116 pKa = 10.58VLGVSGGAGLGATISILAGSSVILSLSLPVVAFVSAVLTLMVVYY160 pKa = 9.7GIARR164 pKa = 11.84QGSSGQPSVYY174 pKa = 10.69SLILSGVIVSSICSSIIMFMVSTASVEE201 pKa = 4.17GLHH204 pKa = 6.58NVIWWMLGSLQPVSTGQQLLSLGLIVSALFGIWLLSPRR242 pKa = 11.84LNALALGRR250 pKa = 11.84EE251 pKa = 4.05MAHH254 pKa = 5.38YY255 pKa = 10.17QGVHH259 pKa = 6.29ADD261 pKa = 4.42LIIVSGLLCATLLAATAVSLSGMIGFVGLIVPHH294 pKa = 6.08VMRR297 pKa = 11.84ALFGPDD303 pKa = 2.84NRR305 pKa = 11.84RR306 pKa = 11.84LVPLSALGGGTFLVLCDD323 pKa = 3.52AVARR327 pKa = 11.84TLMAPVEE334 pKa = 4.25IPVGVVTALAGGPFFLIILQRR355 pKa = 11.84RR356 pKa = 11.84MKK358 pKa = 9.9QAWIAA363 pKa = 3.37

Molecular weight: 38.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2158

0

2158

717850

39

4189

332.6

36.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.715 ± 0.059

1.057 ± 0.021

5.027 ± 0.042

6.666 ± 0.072

4.333 ± 0.045

7.446 ± 0.096

2.131 ± 0.03

6.688 ± 0.051

5.17 ± 0.065

10.386 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.429 ± 0.03

3.717 ± 0.042

4.194 ± 0.043

3.145 ± 0.033

5.664 ± 0.067

7.046 ± 0.073

5.424 ± 0.119

6.723 ± 0.042

1.024 ± 0.021

3.014 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski