Enterovibrio nigricans DSM 22720
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4788 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T4U9M7|A0A1T4U9M7_9GAMM Probable protein kinase UbiB OS=Enterovibrio nigricans DSM 22720 OX=1121868 GN=ubiB PE=3 SV=1
MM1 pKa = 7.9 EE2 pKa = 5.15 YY3 pKa = 8.87 NTSEE7 pKa = 4.31 LCDD10 pKa = 3.5 MYY12 pKa = 11.15 LDD14 pKa = 3.66 QVDD17 pKa = 4.0 VLEE20 pKa = 4.83 PMMNSYY26 pKa = 10.67 GGRR29 pKa = 11.84 SSFGGKK35 pKa = 7.64 ITTVKK40 pKa = 10.39 CFEE43 pKa = 4.91 DD44 pKa = 3.31 NGVIRR49 pKa = 11.84 QVAEE53 pKa = 3.91 QNGEE57 pKa = 4.21 GCVMLIDD64 pKa = 4.42 GGGSLRR70 pKa = 11.84 RR71 pKa = 11.84 ALIDD75 pKa = 3.51 AEE77 pKa = 4.22 IVAMATEE84 pKa = 4.43 NNWEE88 pKa = 4.34 GIVVYY93 pKa = 10.53 GCVRR97 pKa = 11.84 DD98 pKa = 3.94 VDD100 pKa = 4.13 ALDD103 pKa = 3.93 EE104 pKa = 4.29 FDD106 pKa = 5.34 IGVQALASIPVGADD120 pKa = 2.92 SNGIGEE126 pKa = 4.39 TDD128 pKa = 3.47 VPVNFGGVTFIPEE141 pKa = 3.69 DD142 pKa = 3.48 HH143 pKa = 7.02 LYY145 pKa = 11.2 ADD147 pKa = 3.78 TTGVILSQEE156 pKa = 4.51 PIDD159 pKa = 4.36 IEE161 pKa = 4.13
Molecular weight: 17.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.706
IPC_protein 3.681
Toseland 3.478
ProMoST 3.821
Dawson 3.668
Bjellqvist 3.872
Wikipedia 3.592
Rodwell 3.516
Grimsley 3.389
Solomon 3.656
Lehninger 3.617
Nozaki 3.795
DTASelect 3.986
Thurlkill 3.528
EMBOSS 3.605
Sillero 3.808
Patrickios 0.947
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.759
Protein with the highest isoelectric point:
>tr|A0A1T4UWW7|A0A1T4UWW7_9GAMM Anthranilate phosphoribosyltransferase OS=Enterovibrio nigricans DSM 22720 OX=1121868 GN=trpD PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 KK13 pKa = 7.97 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.38 NGRR28 pKa = 11.84 KK29 pKa = 9.44 VIAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.33 GRR39 pKa = 11.84 SKK41 pKa = 10.8 LSKK44 pKa = 10.52
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.705
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.427
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.118
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4788
0
4788
1452864
39
3250
303.4
33.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.913 ± 0.041
1.07 ± 0.014
5.735 ± 0.031
6.238 ± 0.034
4.127 ± 0.024
7.013 ± 0.035
2.224 ± 0.019
6.17 ± 0.027
5.053 ± 0.03
10.146 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.709 ± 0.019
4.207 ± 0.028
4.006 ± 0.02
3.982 ± 0.024
4.771 ± 0.03
6.696 ± 0.035
5.535 ± 0.035
7.225 ± 0.036
1.248 ± 0.016
2.93 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here