Ethiopian tobacco bushy top virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Calvusvirinae; Umbravirus

Average proteome isoelectric point is 7.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L6UUR6|A0A1L6UUR6_9TOMB Replication-associated protein OS=Ethiopian tobacco bushy top virus OX=1538549 PE=4 SV=1
MM1 pKa = 8.1AIRR4 pKa = 11.84AITNWLRR11 pKa = 11.84LDD13 pKa = 3.83YY14 pKa = 11.33SPAPYY19 pKa = 9.36IKK21 pKa = 10.08SRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84CNEE28 pKa = 3.46DD29 pKa = 3.13YY30 pKa = 11.47GEE32 pKa = 3.97XWGLLITQARR42 pKa = 11.84MTEE45 pKa = 4.45SAGHH49 pKa = 6.69HH50 pKa = 4.69MMAWYY55 pKa = 9.59EE56 pKa = 4.04GSAEE60 pKa = 4.23VNGLIPGHH68 pKa = 7.24DD69 pKa = 4.14DD70 pKa = 4.03QGLADD75 pKa = 4.55PAPPPVMEE83 pKa = 4.3VTTPPNEE90 pKa = 4.16EE91 pKa = 4.12VSCPGPSDD99 pKa = 3.4DD100 pKa = 6.1HH101 pKa = 7.49GVEE104 pKa = 3.97WVEE107 pKa = 4.23NLPSPPSPKK116 pKa = 9.89DD117 pKa = 3.08QRR119 pKa = 11.84NRR121 pKa = 11.84LPTLXEE127 pKa = 3.95VLGADD132 pKa = 3.79RR133 pKa = 11.84LQIIPYY139 pKa = 8.39TGGHH143 pKa = 5.7RR144 pKa = 11.84VLSEE148 pKa = 3.84XEE150 pKa = 4.06VRR152 pKa = 11.84PRR154 pKa = 11.84AQGVILLPAPRR165 pKa = 11.84YY166 pKa = 9.65RR167 pKa = 11.84PSLLEE172 pKa = 3.88RR173 pKa = 11.84ALDD176 pKa = 3.6ALVFAWRR183 pKa = 11.84PASQALDD190 pKa = 3.44ALVRR194 pKa = 11.84SQPQEE199 pKa = 4.16DD200 pKa = 4.61GLPASLKK207 pKa = 9.89LQEE210 pKa = 4.32EE211 pKa = 4.91GAPXEE216 pKa = 4.85YY217 pKa = 8.93ITAHH221 pKa = 6.42MIAMEE226 pKa = 4.33LRR228 pKa = 11.84SMFGWQLNTPANRR241 pKa = 11.84EE242 pKa = 3.85LGNRR246 pKa = 11.84VARR249 pKa = 11.84DD250 pKa = 3.42ILRR253 pKa = 11.84DD254 pKa = 3.19RR255 pKa = 11.84CGANRR260 pKa = 11.84EE261 pKa = 4.22NTWYY265 pKa = 10.56LSSLGVQMWFQPTLCDD281 pKa = 3.52LAVKK285 pKa = 10.49AGPQNFF291 pKa = 3.45

Molecular weight: 32.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L6UUR6|A0A1L6UUR6_9TOMB Replication-associated protein OS=Ethiopian tobacco bushy top virus OX=1538549 PE=4 SV=1
MM1 pKa = 7.83AEE3 pKa = 4.22HH4 pKa = 7.06SLLCLVTGRR13 pKa = 11.84PARR16 pKa = 11.84LAADD20 pKa = 3.43NGRR23 pKa = 11.84WVSMTCNINVYY34 pKa = 9.31TSNKK38 pKa = 9.13SSNSRR43 pKa = 11.84RR44 pKa = 11.84APRR47 pKa = 11.84GPVWRR52 pKa = 11.84GHH54 pKa = 5.18RR55 pKa = 11.84QGASRR60 pKa = 11.84NRR62 pKa = 11.84PGRR65 pKa = 11.84VNASQRR71 pKa = 11.84RR72 pKa = 11.84PEE74 pKa = 4.34GGVYY78 pKa = 9.96PPPAPKK84 pKa = 8.79NTKK87 pKa = 9.44QNSGGVAQVPPNPKK101 pKa = 9.44HH102 pKa = 6.55RR103 pKa = 11.84GATIHH108 pKa = 6.9RR109 pKa = 11.84EE110 pKa = 4.26SGCGIHH116 pKa = 6.82PPRR119 pKa = 11.84SRR121 pKa = 11.84RR122 pKa = 11.84CARR125 pKa = 11.84RR126 pKa = 11.84SRR128 pKa = 11.84SVDD131 pKa = 2.94PRR133 pKa = 11.84RR134 pKa = 11.84PTPKK138 pKa = 10.08PKK140 pKa = 10.09QRR142 pKa = 11.84WAAAEE147 pKa = 3.74ISAEE151 pKa = 3.76RR152 pKa = 11.84RR153 pKa = 11.84AEE155 pKa = 3.84IDD157 pKa = 3.5GLLSPLFDD165 pKa = 3.34TLGRR169 pKa = 11.84QVQGDD174 pKa = 3.92AAVLRR179 pKa = 11.84YY180 pKa = 10.09CFGATQRR187 pKa = 11.84QLRR190 pKa = 11.84KK191 pKa = 8.34WRR193 pKa = 11.84KK194 pKa = 7.92PVQPAYY200 pKa = 10.8NVAAPNRR207 pKa = 11.84EE208 pKa = 3.91CSAQLSSTATTNAEE222 pKa = 4.1DD223 pKa = 3.83LQGDD227 pKa = 3.98GKK229 pKa = 11.06GRR231 pKa = 11.84SGQLDD236 pKa = 3.51KK237 pKa = 11.85SEE239 pKa = 4.21VLHH242 pKa = 6.76SGSDD246 pKa = 3.63VPKK249 pKa = 10.21VHH251 pKa = 6.77TDD253 pKa = 3.39CGRR256 pKa = 11.84TATNKK261 pKa = 7.75WW262 pKa = 3.12

Molecular weight: 28.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1656

258

845

414.0

45.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.971 ± 0.661

2.114 ± 0.262

4.831 ± 0.288

5.556 ± 0.505

2.657 ± 0.604

7.367 ± 0.485

2.415 ± 0.095

3.744 ± 0.371

3.623 ± 0.614

8.877 ± 0.77

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.335

4.408 ± 0.479

8.092 ± 0.729

3.925 ± 0.457

8.756 ± 0.827

6.401 ± 0.523

4.408 ± 0.681

6.944 ± 0.569

1.993 ± 0.347

2.778 ± 0.434
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski