Sphingomonas oleivorans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3532 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T5FWB5|A0A2T5FWB5_9SPHN Uncharacterized protein OS=Sphingomonas oleivorans OX=1735121 GN=CLG96_13015 PE=4 SV=1
MM1 pKa = 7.16IVAYY5 pKa = 8.49PLRR8 pKa = 11.84LFLDD12 pKa = 4.58CSTAHH17 pKa = 7.29LSQAARR23 pKa = 11.84AYY25 pKa = 9.71IDD27 pKa = 3.33RR28 pKa = 11.84TDD30 pKa = 3.6VVASPTPYY38 pKa = 10.81GWFVWASEE46 pKa = 4.25EE47 pKa = 4.35PGDD50 pKa = 4.8GVPPDD55 pKa = 4.49LMAIMAQARR64 pKa = 11.84SLGAEE69 pKa = 4.04YY70 pKa = 11.45VNFDD74 pKa = 4.14CDD76 pKa = 3.57ADD78 pKa = 4.5EE79 pKa = 5.12IDD81 pKa = 4.7GLQTFDD87 pKa = 3.2WDD89 pKa = 3.46

Molecular weight: 9.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T5FVH1|A0A2T5FVH1_9SPHN Uncharacterized protein OS=Sphingomonas oleivorans OX=1735121 GN=CLG96_11415 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84SATPGGRR28 pKa = 11.84KK29 pKa = 9.04VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 6.76TLSAA44 pKa = 4.22

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3532

0

3532

1144095

33

2389

323.9

35.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.706 ± 0.067

0.727 ± 0.012

5.809 ± 0.032

5.427 ± 0.039

3.552 ± 0.027

8.95 ± 0.04

1.999 ± 0.021

5.238 ± 0.027

2.665 ± 0.03

10.321 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.312 ± 0.019

2.4 ± 0.028

5.434 ± 0.033

2.885 ± 0.023

7.906 ± 0.037

5.22 ± 0.03

4.963 ± 0.029

6.846 ± 0.031

1.375 ± 0.018

2.264 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski