Clostridiales bacterium AM23-16LB

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; unclassified Eubacteriales

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3413 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A373BHK3|A0A373BHK3_9FIRM Site-specific integrase OS=Clostridiales bacterium AM23-16LB OX=2306574 GN=DW644_07180 PE=4 SV=1
MM1 pKa = 7.07NRR3 pKa = 11.84KK4 pKa = 7.26TLFCFLTALIMIFVLAGCSGKK25 pKa = 10.21NSGGNIDD32 pKa = 4.19NVQIPDD38 pKa = 3.59WKK40 pKa = 10.24PSEE43 pKa = 4.49IYY45 pKa = 10.37SDD47 pKa = 3.72SDD49 pKa = 3.24IEE51 pKa = 4.76AAFQTVKK58 pKa = 10.83DD59 pKa = 3.89YY60 pKa = 11.24FGNEE64 pKa = 3.64FDD66 pKa = 4.53GCTLTKK72 pKa = 10.51LSYY75 pKa = 10.76PGDD78 pKa = 3.69TYY80 pKa = 11.72ADD82 pKa = 4.05EE83 pKa = 4.38FDD85 pKa = 3.95EE86 pKa = 4.73WAEE89 pKa = 4.12QYY91 pKa = 10.85DD92 pKa = 3.84ADD94 pKa = 4.06EE95 pKa = 5.71AIVILSSFDD104 pKa = 3.37VDD106 pKa = 3.7SSGGDD111 pKa = 3.21GSLNPDD117 pKa = 2.97STYY120 pKa = 11.61DD121 pKa = 3.2DD122 pKa = 3.88WKK124 pKa = 10.55WILIRR129 pKa = 11.84NDD131 pKa = 3.03SGNWEE136 pKa = 4.45HH137 pKa = 6.93VDD139 pKa = 2.97HH140 pKa = 7.19GYY142 pKa = 11.09

Molecular weight: 15.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A373B2B0|A0A373B2B0_9FIRM FUSC family protein OS=Clostridiales bacterium AM23-16LB OX=2306574 GN=DW644_15435 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.7VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.68GRR39 pKa = 11.84KK40 pKa = 8.28QLSAA44 pKa = 3.9

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3413

0

3413

1051039

27

4428

308.0

34.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.634 ± 0.042

1.521 ± 0.017

5.712 ± 0.036

7.756 ± 0.049

3.843 ± 0.03

7.123 ± 0.041

1.867 ± 0.022

6.842 ± 0.045

6.56 ± 0.04

9.133 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.165 ± 0.028

3.984 ± 0.033

3.402 ± 0.024

3.58 ± 0.026

4.64 ± 0.04

5.615 ± 0.035

5.612 ± 0.062

6.955 ± 0.033

0.977 ± 0.016

4.08 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski