Amino acid dipepetide frequency for Clostridiales bacterium AM23-16LB

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.5AlaAla: 7.5 ± 0.128	1.244AlaCys: 1.244 ± 0.04	4.923AlaAsp: 4.923 ± 0.081	5.833AlaGlu: 5.833 ± 0.09	2.918AlaPhe: 2.918 ± 0.063	6.244AlaGly: 6.244 ± 0.098	1.211AlaHis: 1.211 ± 0.031	4.874AlaIle: 4.874 ± 0.089	4.694AlaLys: 4.694 ± 0.076	6.834AlaLeu: 6.834 ± 0.097	2.414AlaMet: 2.414 ± 0.048	2.457AlaAsn: 2.457 ± 0.058	2.131AlaPro: 2.131 ± 0.054	2.568AlaGln: 2.568 ± 0.052	3.084AlaArg: 3.084 ± 0.06	3.99AlaSer: 3.99 ± 0.067	3.493AlaThr: 3.493 ± 0.077	6.353AlaVal: 6.353 ± 0.088	0.656AlaTrp: 0.656 ± 0.025	2.92AlaTyr: 2.92 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	1.086CysAla: 1.086 ± 0.034	0.298CysCys: 0.298 ± 0.018	0.837CysAsp: 0.837 ± 0.027	0.922CysGlu: 0.922 ± 0.035	0.613CysPhe: 0.613 ± 0.027	1.601CysGly: 1.601 ± 0.046	0.325CysHis: 0.325 ± 0.016	1.031CysIle: 1.031 ± 0.031	0.814CysLys: 0.814 ± 0.027	1.212CysLeu: 1.212 ± 0.035	0.48CysMet: 0.48 ± 0.02	0.51CysAsn: 0.51 ± 0.022	0.659CysPro: 0.659 ± 0.026	0.521CysGln: 0.521 ± 0.024	0.785CysArg: 0.785 ± 0.034	0.86CysSer: 0.86 ± 0.028	0.787CysThr: 0.787 ± 0.026	1.154CysVal: 1.154 ± 0.035	0.143CysTrp: 0.143 ± 0.012	0.568CysTyr: 0.568 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	4.446AspAla: 4.446 ± 0.078	0.876AspCys: 0.876 ± 0.028	2.89AspAsp: 2.89 ± 0.067	4.675AspGlu: 4.675 ± 0.06	2.392AspPhe: 2.392 ± 0.058	4.686AspGly: 4.686 ± 0.086	1.081AspHis: 1.081 ± 0.035	3.732AspIle: 3.732 ± 0.065	3.227AspLys: 3.227 ± 0.059	5.026AspLeu: 5.026 ± 0.082	1.822AspMet: 1.822 ± 0.038	1.992AspAsn: 1.992 ± 0.046	2.087AspPro: 2.087 ± 0.046	1.935AspGln: 1.935 ± 0.041	2.802AspArg: 2.802 ± 0.059	2.942AspSer: 2.942 ± 0.059	3.134AspThr: 3.134 ± 0.067	3.971AspVal: 3.971 ± 0.067	0.657AspTrp: 0.657 ± 0.027	2.744AspTyr: 2.744 ± 0.056	0.0AspXaa: 0.0 ± 0.0
Glu	5.847GluAla: 5.847 ± 0.08	0.772GluCys: 0.772 ± 0.026	4.634GluAsp: 4.634 ± 0.067	7.254GluGlu: 7.254 ± 0.113	2.501GluPhe: 2.501 ± 0.047	4.358GluGly: 4.358 ± 0.075	1.602GluHis: 1.602 ± 0.04	5.315GluIle: 5.315 ± 0.089	6.598GluLys: 6.598 ± 0.087	7.3GluLeu: 7.3 ± 0.105	2.499GluMet: 2.499 ± 0.051	3.804GluAsn: 3.804 ± 0.065	2.117GluPro: 2.117 ± 0.05	3.649GluGln: 3.649 ± 0.079	3.681GluArg: 3.681 ± 0.072	3.294GluSer: 3.294 ± 0.067	3.935GluThr: 3.935 ± 0.075	4.538GluVal: 4.538 ± 0.066	0.705GluTrp: 0.705 ± 0.028	3.155GluTyr: 3.155 ± 0.056	0.0GluXaa: 0.0 ± 0.0
Phe	2.806PheAla: 2.806 ± 0.048	0.775PheCys: 0.775 ± 0.029	2.418PheAsp: 2.418 ± 0.047	2.45PheGlu: 2.45 ± 0.049	1.619PhePhe: 1.619 ± 0.05	2.927PheGly: 2.927 ± 0.054	0.885PheHis: 0.885 ± 0.031	2.329PheIle: 2.329 ± 0.054	1.651PheLys: 1.651 ± 0.04	3.919PheLeu: 3.919 ± 0.084	1.072PheMet: 1.072 ± 0.033	1.306PheAsn: 1.306 ± 0.037	1.403PhePro: 1.403 ± 0.033	1.396PheGln: 1.396 ± 0.038	1.791PheArg: 1.791 ± 0.043	2.638PheSer: 2.638 ± 0.059	2.274PheThr: 2.274 ± 0.052	2.734PheVal: 2.734 ± 0.049	0.422PheTrp: 0.422 ± 0.022	1.609PheTyr: 1.609 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	5.135GlyAla: 5.135 ± 0.081	1.315GlyCys: 1.315 ± 0.043	3.482GlyAsp: 3.482 ± 0.07	4.693GlyGlu: 4.693 ± 0.073	2.886GlyPhe: 2.886 ± 0.051	4.859GlyGly: 4.859 ± 0.098	1.314GlyHis: 1.314 ± 0.038	6.027GlyIle: 6.027 ± 0.082	5.382GlyLys: 5.382 ± 0.079	5.822GlyLeu: 5.822 ± 0.086	2.586GlyMet: 2.586 ± 0.06	3.114GlyAsn: 3.114 ± 0.083	1.264GlyPro: 1.264 ± 0.038	2.264GlyGln: 2.264 ± 0.045	3.177GlyArg: 3.177 ± 0.062	4.295GlySer: 4.295 ± 0.079	4.617GlyThr: 4.617 ± 0.098	4.928GlyVal: 4.928 ± 0.067	0.768GlyTrp: 0.768 ± 0.024	3.299GlyTyr: 3.299 ± 0.07	0.0GlyXaa: 0.0 ± 0.0
His	1.263HisAla: 1.263 ± 0.034	0.341HisCys: 0.341 ± 0.016	0.969HisAsp: 0.969 ± 0.042	1.254HisGlu: 1.254 ± 0.034	0.869HisPhe: 0.869 ± 0.026	1.398HisGly: 1.398 ± 0.044	0.453HisHis: 0.453 ± 0.031	1.339HisIle: 1.339 ± 0.034	0.95HisLys: 0.95 ± 0.03	1.714HisLeu: 1.714 ± 0.046	0.621HisMet: 0.621 ± 0.022	0.772HisAsn: 0.772 ± 0.026	0.981HisPro: 0.981 ± 0.032	0.637HisGln: 0.637 ± 0.026	0.949HisArg: 0.949 ± 0.033	0.896HisSer: 0.896 ± 0.033	1.059HisThr: 1.059 ± 0.036	1.293HisVal: 1.293 ± 0.036	0.176HisTrp: 0.176 ± 0.012	0.735HisTyr: 0.735 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.256IleAla: 5.256 ± 0.08	1.34IleCys: 1.34 ± 0.037	3.828IleAsp: 3.828 ± 0.064	4.333IleGlu: 4.333 ± 0.061	2.743IlePhe: 2.743 ± 0.063	4.764IleGly: 4.764 ± 0.073	1.389IleHis: 1.389 ± 0.04	4.328IleIle: 4.328 ± 0.069	3.561IleLys: 3.561 ± 0.056	6.695IleLeu: 6.695 ± 0.098	1.914IleMet: 1.914 ± 0.046	2.527IleAsn: 2.527 ± 0.058	3.092IlePro: 3.092 ± 0.053	2.432IleGln: 2.432 ± 0.049	3.773IleArg: 3.773 ± 0.065	4.538IleSer: 4.538 ± 0.074	3.897IleThr: 3.897 ± 0.058	4.605IleVal: 4.605 ± 0.072	0.649IleTrp: 0.649 ± 0.024	2.752IleTyr: 2.752 ± 0.059	0.0IleXaa: 0.0 ± 0.0
Lys	5.017LysAla: 5.017 ± 0.077	0.652LysCys: 0.652 ± 0.024	3.903LysAsp: 3.903 ± 0.063	6.387LysGlu: 6.387 ± 0.094	1.762LysPhe: 1.762 ± 0.043	4.011LysGly: 4.011 ± 0.07	1.007LysHis: 1.007 ± 0.027	4.441LysIle: 4.441 ± 0.072	5.905LysLys: 5.905 ± 0.082	5.408LysLeu: 5.408 ± 0.071	2.129LysMet: 2.129 ± 0.049	3.206LysAsn: 3.206 ± 0.061	2.067LysPro: 2.067 ± 0.042	2.366LysGln: 2.366 ± 0.056	2.996LysArg: 2.996 ± 0.053	3.143LysSer: 3.143 ± 0.056	3.662LysThr: 3.662 ± 0.072	4.283LysVal: 4.283 ± 0.067	0.605LysTrp: 0.605 ± 0.023	2.647LysTyr: 2.647 ± 0.052	0.0LysXaa: 0.0 ± 0.0
Leu	7.081LeuAla: 7.081 ± 0.088	1.554LeuCys: 1.554 ± 0.039	5.142LeuAsp: 5.142 ± 0.082	6.537LeuGlu: 6.537 ± 0.11	3.62LeuPhe: 3.62 ± 0.071	5.967LeuGly: 5.967 ± 0.09	1.696LeuHis: 1.696 ± 0.04	5.745LeuIle: 5.745 ± 0.088	6.206LeuLys: 6.206 ± 0.08	9.146LeuLeu: 9.146 ± 0.144	2.691LeuMet: 2.691 ± 0.051	3.649LeuAsn: 3.649 ± 0.064	3.633LeuPro: 3.633 ± 0.057	3.153LeuGln: 3.153 ± 0.059	3.917LeuArg: 3.917 ± 0.074	6.114LeuSer: 6.114 ± 0.088	5.63LeuThr: 5.63 ± 0.083	5.652LeuVal: 5.652 ± 0.08	0.851LeuTrp: 0.851 ± 0.027	3.35LeuTyr: 3.35 ± 0.059	0.0LeuXaa: 0.0 ± 0.0
Met	2.518MetAla: 2.518 ± 0.053	0.347MetCys: 0.347 ± 0.019	1.959MetAsp: 1.959 ± 0.037	2.89MetGlu: 2.89 ± 0.063	1.008MetPhe: 1.008 ± 0.031	2.165MetGly: 2.165 ± 0.044	0.459MetHis: 0.459 ± 0.021	2.12MetIle: 2.12 ± 0.05	2.62MetLys: 2.62 ± 0.049	2.811MetLeu: 2.811 ± 0.064	1.004MetMet: 1.004 ± 0.036	1.495MetAsn: 1.495 ± 0.041	1.178MetPro: 1.178 ± 0.034	1.14MetGln: 1.14 ± 0.035	1.319MetArg: 1.319 ± 0.035	1.729MetSer: 1.729 ± 0.045	1.81MetThr: 1.81 ± 0.047	1.966MetVal: 1.966 ± 0.043	0.23MetTrp: 0.23 ± 0.014	0.886MetTyr: 0.886 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	2.971AsnAla: 2.971 ± 0.055	0.587AsnCys: 0.587 ± 0.023	1.914AsnAsp: 1.914 ± 0.045	2.65AsnGlu: 2.65 ± 0.049	1.37AsnPhe: 1.37 ± 0.034	3.357AsnGly: 3.357 ± 0.082	0.837AsnHis: 0.837 ± 0.031	2.945AsnIle: 2.945 ± 0.05	2.157AsnLys: 2.157 ± 0.049	3.616AsnLeu: 3.616 ± 0.065	1.287AsnMet: 1.287 ± 0.036	1.562AsnAsn: 1.562 ± 0.049	1.847AsnPro: 1.847 ± 0.044	1.479AsnGln: 1.479 ± 0.043	2.088AsnArg: 2.088 ± 0.048	2.091AsnSer: 2.091 ± 0.058	2.164AsnThr: 2.164 ± 0.058	2.76AsnVal: 2.76 ± 0.053	0.425AsnTrp: 0.425 ± 0.018	1.734AsnTyr: 1.734 ± 0.043	0.0AsnXaa: 0.0 ± 0.0
Pro	2.648ProAla: 2.648 ± 0.055	0.442ProCys: 0.442 ± 0.02	2.417ProAsp: 2.417 ± 0.048	3.537ProGlu: 3.537 ± 0.068	1.547ProPhe: 1.547 ± 0.042	2.417ProGly: 2.417 ± 0.051	0.611ProHis: 0.611 ± 0.027	2.014ProIle: 2.014 ± 0.044	1.99ProLys: 1.99 ± 0.042	2.792ProLeu: 2.792 ± 0.048	0.95ProMet: 0.95 ± 0.029	1.142ProAsn: 1.142 ± 0.04	0.814ProPro: 0.814 ± 0.035	1.116ProGln: 1.116 ± 0.042	1.09ProArg: 1.09 ± 0.032	1.838ProSer: 1.838 ± 0.041	1.733ProThr: 1.733 ± 0.044	3.135ProVal: 3.135 ± 0.054	0.351ProTrp: 0.351 ± 0.016	1.436ProTyr: 1.436 ± 0.036	0.001ProXaa: 0.001 ± 0.001
Gln	2.726GlnAla: 2.726 ± 0.052	0.375GlnCys: 0.375 ± 0.019	1.733GlnAsp: 1.733 ± 0.044	3.166GlnGlu: 3.166 ± 0.068	1.169GlnPhe: 1.169 ± 0.029	2.036GlnGly: 2.036 ± 0.04	0.547GlnHis: 0.547 ± 0.02	2.924GlnIle: 2.924 ± 0.054	2.941GlnLys: 2.941 ± 0.068	3.001GlnLeu: 3.001 ± 0.054	1.4GlnMet: 1.4 ± 0.038	1.615GlnAsn: 1.615 ± 0.041	1.039GlnPro: 1.039 ± 0.038	1.451GlnGln: 1.451 ± 0.046	1.588GlnArg: 1.588 ± 0.045	1.811GlnSer: 1.811 ± 0.044	2.064GlnThr: 2.064 ± 0.047	2.374GlnVal: 2.374 ± 0.056	0.37GlnTrp: 0.37 ± 0.017	1.475GlnTyr: 1.475 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	2.852ArgAla: 2.852 ± 0.057	0.621ArgCys: 0.621 ± 0.028	2.246ArgAsp: 2.246 ± 0.049	3.912ArgGlu: 3.912 ± 0.076	1.841ArgPhe: 1.841 ± 0.048	2.549ArgGly: 2.549 ± 0.052	0.871ArgHis: 0.871 ± 0.032	3.513ArgIle: 3.513 ± 0.062	3.724ArgLys: 3.724 ± 0.069	4.314ArgLeu: 4.314 ± 0.088	1.708ArgMet: 1.708 ± 0.045	1.949ArgAsn: 1.949 ± 0.042	1.506ArgPro: 1.506 ± 0.044	2.068ArgGln: 2.068 ± 0.049	2.662ArgArg: 2.662 ± 0.058	2.438ArgSer: 2.438 ± 0.049	2.531ArgThr: 2.531 ± 0.051	2.755ArgVal: 2.755 ± 0.061	0.44ArgTrp: 0.44 ± 0.022	1.904ArgTyr: 1.904 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	4.128SerAla: 4.128 ± 0.076	0.855SerCys: 0.855 ± 0.031	3.298SerAsp: 3.298 ± 0.066	3.91SerGlu: 3.91 ± 0.069	2.446SerPhe: 2.446 ± 0.052	4.906SerGly: 4.906 ± 0.085	1.095SerHis: 1.095 ± 0.033	3.841SerIle: 3.841 ± 0.079	2.973SerLys: 2.973 ± 0.062	4.902SerLeu: 4.902 ± 0.07	1.919SerMet: 1.919 ± 0.04	2.059SerAsn: 2.059 ± 0.049	1.712SerPro: 1.712 ± 0.04	1.823SerGln: 1.823 ± 0.047	2.894SerArg: 2.894 ± 0.062	3.337SerSer: 3.337 ± 0.077	2.894SerThr: 2.894 ± 0.07	4.248SerVal: 4.248 ± 0.076	0.557SerTrp: 0.557 ± 0.025	2.357SerTyr: 2.357 ± 0.058	0.0SerXaa: 0.0 ± 0.0
Thr	4.832ThrAla: 4.832 ± 0.089	0.728ThrCys: 0.728 ± 0.026	3.536ThrAsp: 3.536 ± 0.088	4.245ThrGlu: 4.245 ± 0.076	2.082ThrPhe: 2.082 ± 0.045	4.957ThrGly: 4.957 ± 0.097	0.957ThrHis: 0.957 ± 0.03	3.713ThrIle: 3.713 ± 0.065	2.845ThrLys: 2.845 ± 0.057	5.001ThrLeu: 5.001 ± 0.075	1.579ThrMet: 1.579 ± 0.033	1.845ThrAsn: 1.845 ± 0.054	2.28ThrPro: 2.28 ± 0.055	1.569ThrGln: 1.569 ± 0.043	2.205ThrArg: 2.205 ± 0.051	3.084ThrSer: 3.084 ± 0.068	3.042ThrThr: 3.042 ± 0.097	4.738ThrVal: 4.738 ± 0.09	0.568ThrTrp: 0.568 ± 0.026	2.312ThrTyr: 2.312 ± 0.074	0.0ThrXaa: 0.0 ± 0.0
Val	4.809ValAla: 4.809 ± 0.082	1.322ValCys: 1.322 ± 0.038	3.944ValAsp: 3.944 ± 0.073	4.862ValGlu: 4.862 ± 0.063	2.886ValPhe: 2.886 ± 0.056	4.351ValGly: 4.351 ± 0.078	1.221ValHis: 1.221 ± 0.038	4.935ValIle: 4.935 ± 0.078	4.376ValLys: 4.376 ± 0.068	6.979ValLeu: 6.979 ± 0.085	2.11ValMet: 2.11 ± 0.048	2.737ValAsn: 2.737 ± 0.055	2.515ValPro: 2.515 ± 0.049	2.243ValGln: 2.243 ± 0.049	3.104ValArg: 3.104 ± 0.051	4.445ValSer: 4.445 ± 0.07	4.536ValThr: 4.536 ± 0.091	4.855ValVal: 4.855 ± 0.081	0.689ValTrp: 0.689 ± 0.026	2.631ValTyr: 2.631 ± 0.055	0.0ValXaa: 0.0 ± 0.0
Trp	0.513TrpAla: 0.513 ± 0.025	0.149TrpCys: 0.149 ± 0.011	0.519TrpAsp: 0.519 ± 0.022	0.752TrpGlu: 0.752 ± 0.026	0.464TrpPhe: 0.464 ± 0.024	0.66TrpGly: 0.66 ± 0.026	0.189TrpHis: 0.189 ± 0.014	0.712TrpIle: 0.712 ± 0.031	0.864TrpLys: 0.864 ± 0.026	0.971TrpLeu: 0.971 ± 0.034	0.373TrpMet: 0.373 ± 0.019	0.562TrpAsn: 0.562 ± 0.023	0.197TrpPro: 0.197 ± 0.014	0.406TrpGln: 0.406 ± 0.019	0.409TrpArg: 0.409 ± 0.021	0.498TrpSer: 0.498 ± 0.022	0.464TrpThr: 0.464 ± 0.024	0.535TrpVal: 0.535 ± 0.022	0.107TrpTrp: 0.107 ± 0.01	0.429TrpTyr: 0.429 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.91TyrAla: 2.91 ± 0.054	0.614TyrCys: 0.614 ± 0.024	2.529TyrAsp: 2.529 ± 0.072	3.297TyrGlu: 3.297 ± 0.064	1.69TyrPhe: 1.69 ± 0.042	3.016TyrGly: 3.016 ± 0.054	0.915TyrHis: 0.915 ± 0.033	2.539TyrIle: 2.539 ± 0.054	2.118TyrLys: 2.118 ± 0.052	3.868TyrLeu: 3.868 ± 0.066	1.09TyrMet: 1.09 ± 0.034	1.539TyrAsn: 1.539 ± 0.038	1.499TyrPro: 1.499 ± 0.038	1.587TyrGln: 1.587 ± 0.042	2.089TyrArg: 2.089 ± 0.042	2.174TyrSer: 2.174 ± 0.052	2.392TyrThr: 2.392 ± 0.074	2.669TyrVal: 2.669 ± 0.059	0.405TyrTrp: 0.405 ± 0.021	1.857TyrTyr: 1.857 ± 0.055	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.002

Statistics based on 3413 proteins (1051039 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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