Burkholderiales bacterium LSUCC0115

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; unclassified Burkholderiales

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1762 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7C9GS29|A0A7C9GS29_9BURK Exodeoxyribonuclease III OS=Burkholderiales bacterium LSUCC0115 OX=1778438 GN=xth PE=3 SV=1
MM1 pKa = 7.4TLRR4 pKa = 11.84VYY6 pKa = 10.18YY7 pKa = 11.08VNGFDD12 pKa = 4.43EE13 pKa = 6.17GEE15 pKa = 4.14DD16 pKa = 3.6PQYY19 pKa = 11.56VGLEE23 pKa = 3.73HH24 pKa = 7.45AIYY27 pKa = 10.89YY28 pKa = 9.97SDD30 pKa = 4.04RR31 pKa = 11.84NSNTGTWSDD40 pKa = 3.44RR41 pKa = 11.84APIKK45 pKa = 10.54IEE47 pKa = 3.82GQIDD51 pKa = 3.81GNAVDD56 pKa = 4.52PNLIQLEE63 pKa = 4.22DD64 pKa = 3.35GRR66 pKa = 11.84YY67 pKa = 9.49LLTYY71 pKa = 9.71MRR73 pKa = 11.84GQFTQTNPEE82 pKa = 4.18PVSTIYY88 pKa = 10.7SAWSYY93 pKa = 11.48NGVDD97 pKa = 4.26FYY99 pKa = 11.84NPQVLYY105 pKa = 10.4KK106 pKa = 9.86PSSQASFGLPAVTDD120 pKa = 3.97PSLVQLTDD128 pKa = 3.87GSWLLAISNPSSQSAGLYY146 pKa = 8.54TSSDD150 pKa = 3.21GVNFTANGVSLPAFSPDD167 pKa = 3.28FQLLANGNVRR177 pKa = 11.84IVYY180 pKa = 10.41ADD182 pKa = 3.46AAAGGFGSQVSSDD195 pKa = 3.65GGEE198 pKa = 4.0TWVVEE203 pKa = 4.31SGARR207 pKa = 11.84FAGPYY212 pKa = 9.42FDD214 pKa = 4.69PSVFRR219 pKa = 11.84MPDD222 pKa = 3.3GSWGMFYY229 pKa = 9.36KK230 pKa = 9.95TQTTVEE236 pKa = 4.41GQQSSPLLGHH246 pKa = 6.73LTSLATSSDD255 pKa = 2.9GTTFTTQDD263 pKa = 3.12AEE265 pKa = 4.23FAAAASVAEE274 pKa = 5.14GIDD277 pKa = 3.93FSGLTVSEE285 pKa = 4.23SLIAATSTNDD295 pKa = 3.5TIAVGEE301 pKa = 4.15QGAHH305 pKa = 3.87VRR307 pKa = 11.84AGNGIDD313 pKa = 3.32TVSFAIASSALTLEE327 pKa = 3.63QDD329 pKa = 4.53FFNEE333 pKa = 4.15GDD335 pKa = 3.6WVGHH339 pKa = 6.23LLSDD343 pKa = 4.16ANVAYY348 pKa = 10.72LLQDD352 pKa = 2.88VEE354 pKa = 4.99RR355 pKa = 11.84FVFTDD360 pKa = 3.1KK361 pKa = 11.2SLAVDD366 pKa = 4.37LDD368 pKa = 3.7GHH370 pKa = 6.68AGQVAKK376 pKa = 10.67ILGAVFGSDD385 pKa = 2.68SVANAEE391 pKa = 4.18YY392 pKa = 10.89VGIGLDD398 pKa = 3.73LLDD401 pKa = 5.07GGMSYY406 pKa = 11.48SDD408 pKa = 3.83LAALAVSVTGNTSSTDD424 pKa = 2.75ICNLLWEE431 pKa = 4.35NVIGSSATATDD442 pKa = 3.51IAPFKK447 pKa = 11.31SMLDD451 pKa = 3.42TGQLSIGQLTTLAADD466 pKa = 3.67TSFNTDD472 pKa = 3.13NIDD475 pKa = 3.62LVGLSEE481 pKa = 4.91IGLEE485 pKa = 4.63FII487 pKa = 5.54

Molecular weight: 51.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7C9GTN5|A0A7C9GTN5_9BURK Uncharacterized protein OS=Burkholderiales bacterium LSUCC0115 OX=1778438 GN=GH816_07535 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.6RR14 pKa = 11.84THH16 pKa = 5.75GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.67GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1762

0

1762

579727

37

1818

329.0

35.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.457 ± 0.06

0.952 ± 0.021

5.311 ± 0.044

5.143 ± 0.052

3.6 ± 0.04

8.177 ± 0.05

2.148 ± 0.03

4.834 ± 0.044

3.869 ± 0.055

10.858 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.648 ± 0.027

2.661 ± 0.033

4.952 ± 0.039

4.827 ± 0.045

6.132 ± 0.049

6.156 ± 0.05

5.061 ± 0.043

7.623 ± 0.046

1.426 ± 0.027

2.163 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski