Edhazardia aedis (strain USNM 41457) (Microsporidian parasite)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Microsporidia incertae sedis; Culicosporidae; Edhazardia; Edhazardia aedis

Average proteome isoelectric point is 7.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4189 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9DT00|J9DT00_EDHAE RING-type domain-containing protein OS=Edhazardia aedis (strain USNM 41457) OX=1003232 GN=EDEG_01312 PE=4 SV=1
SS1 pKa = 6.83VEE3 pKa = 4.37TPDD6 pKa = 3.58QTGEE10 pKa = 3.92DD11 pKa = 3.69AEE13 pKa = 4.31NPEE16 pKa = 4.24EE17 pKa = 4.23KK18 pKa = 10.53LEE20 pKa = 4.16KK21 pKa = 9.93TSEE24 pKa = 4.19SPEE27 pKa = 4.04NPEE30 pKa = 4.31NNPDD34 pKa = 3.43QAIEE38 pKa = 3.91NAEE41 pKa = 4.05EE42 pKa = 4.0NGKK45 pKa = 9.59SEE47 pKa = 4.38EE48 pKa = 4.22NLDD51 pKa = 3.55QATEE55 pKa = 4.02NTEE58 pKa = 4.18DD59 pKa = 3.94PEE61 pKa = 4.45KK62 pKa = 11.28SEE64 pKa = 5.96DD65 pKa = 3.56NTDD68 pKa = 3.41QTNEE72 pKa = 3.72QSEE75 pKa = 4.42NPEE78 pKa = 4.58DD79 pKa = 4.36NPDD82 pKa = 3.26QAGEE86 pKa = 4.17GNEE89 pKa = 4.14KK90 pKa = 10.52PEE92 pKa = 4.19EE93 pKa = 3.87QCEE96 pKa = 4.54CNDD99 pKa = 3.43HH100 pKa = 6.55PVNEE104 pKa = 4.39LEE106 pKa = 4.27EE107 pKa = 4.61NQDD110 pKa = 3.33QNCQDD115 pKa = 4.34EE116 pKa = 4.83EE117 pKa = 4.81CSCDD121 pKa = 3.31DD122 pKa = 3.79HH123 pKa = 9.25SPNEE127 pKa = 4.33DD128 pKa = 3.92EE129 pKa = 5.31EE130 pKa = 4.98CSCDD134 pKa = 3.37DD135 pKa = 3.79HH136 pKa = 9.25SPNEE140 pKa = 4.33DD141 pKa = 3.92EE142 pKa = 5.31EE143 pKa = 4.98CSCDD147 pKa = 3.37DD148 pKa = 3.79HH149 pKa = 9.38SPNEE153 pKa = 4.49DD154 pKa = 3.8EE155 pKa = 5.53NCEE158 pKa = 4.08CDD160 pKa = 3.89DD161 pKa = 4.01HH162 pKa = 9.73CSDD165 pKa = 3.9EE166 pKa = 5.8DD167 pKa = 4.2EE168 pKa = 4.82NCEE171 pKa = 4.69CDD173 pKa = 3.89DD174 pKa = 4.01HH175 pKa = 9.73CSDD178 pKa = 3.9EE179 pKa = 5.8DD180 pKa = 4.2EE181 pKa = 4.82NCEE184 pKa = 4.95CDD186 pKa = 3.97DD187 pKa = 4.19HH188 pKa = 9.22CPDD191 pKa = 3.63EE192 pKa = 5.93DD193 pKa = 4.54EE194 pKa = 5.21NCEE197 pKa = 4.26CDD199 pKa = 3.89DD200 pKa = 4.01HH201 pKa = 9.73CSDD204 pKa = 3.9EE205 pKa = 5.8DD206 pKa = 4.2EE207 pKa = 4.82NCEE210 pKa = 4.95CDD212 pKa = 3.97DD213 pKa = 4.19HH214 pKa = 9.22CPDD217 pKa = 3.63EE218 pKa = 5.93DD219 pKa = 4.54EE220 pKa = 5.21NCEE223 pKa = 4.09CDD225 pKa = 4.24DD226 pKa = 4.84HH227 pKa = 9.74SGDD230 pKa = 3.79TNEE233 pKa = 4.68TKK235 pKa = 10.55DD236 pKa = 4.18ASGSNNGAEE245 pKa = 4.07NGEE248 pKa = 4.02NLDD251 pKa = 4.01RR252 pKa = 11.84SASHH256 pKa = 6.4PTNPSSNEE264 pKa = 3.76NFQKK268 pKa = 10.69NANNNSPINGSEE280 pKa = 3.88NNPLMKK286 pKa = 10.54NPQDD290 pKa = 3.43QKK292 pKa = 11.73QNLKK296 pKa = 10.17KK297 pKa = 10.05QQNRR301 pKa = 11.84NNSPFCGHH309 pKa = 5.83TPYY312 pKa = 10.64FVFTNRR318 pKa = 11.84CHH320 pKa = 5.5GHH322 pKa = 5.86CGGYY326 pKa = 9.56RR327 pKa = 11.84PKK329 pKa = 10.71NSCKK333 pKa = 10.52

Molecular weight: 37.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J8ZW17|J8ZW17_EDHAE Uncharacterized protein OS=Edhazardia aedis (strain USNM 41457) OX=1003232 GN=EDEG_01826 PE=4 SV=1
MM1 pKa = 6.94VFKK4 pKa = 10.68IIARR8 pKa = 11.84EE9 pKa = 4.06SKK11 pKa = 9.63HH12 pKa = 5.13LSRR15 pKa = 11.84RR16 pKa = 11.84EE17 pKa = 3.99SLVQTSHH24 pKa = 6.82KK25 pKa = 10.43FSFLSSILFRR35 pKa = 11.84CLFFKK40 pKa = 10.65NIKK43 pKa = 9.76ILFFNFRR50 pKa = 11.84FFSSLKK56 pKa = 9.29LQIVSSSLPEE66 pKa = 3.82RR67 pKa = 11.84VFVSLDD73 pKa = 3.32LPIIIIITLTNMSRR87 pKa = 11.84NLILRR92 pKa = 11.84PCHH95 pKa = 7.03IYY97 pKa = 9.94EE98 pKa = 4.26QNFF101 pKa = 3.06

Molecular weight: 12.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4189

0

4189

1478472

45

4121

352.9

41.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.239 ± 0.034

1.976 ± 0.017

5.783 ± 0.03

6.852 ± 0.043

5.891 ± 0.041

3.001 ± 0.032

1.933 ± 0.015

9.256 ± 0.036

10.545 ± 0.04

8.633 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.984 ± 0.015

9.999 ± 0.094

2.387 ± 0.029

3.224 ± 0.022

3.293 ± 0.024

7.891 ± 0.038

4.95 ± 0.025

4.51 ± 0.027

0.431 ± 0.007

4.223 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski