Amino acid dipepetide frequency for Edhazardia aedis (strain USNM 41457) (Microsporidian parasite)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	1.491AlaAla: 1.491 ± 0.074	0.594AlaCys: 0.594 ± 0.022	1.815AlaAsp: 1.815 ± 0.037	2.319AlaGlu: 2.319 ± 0.054	1.844AlaPhe: 1.844 ± 0.038	1.193AlaGly: 1.193 ± 0.044	0.639AlaHis: 0.639 ± 0.022	2.943AlaIle: 2.943 ± 0.048	3.147AlaLys: 3.147 ± 0.058	3.0AlaLeu: 3.0 ± 0.061	0.624AlaMet: 0.624 ± 0.021	2.591AlaAsn: 2.591 ± 0.052	0.783AlaPro: 0.783 ± 0.03	1.163AlaGln: 1.163 ± 0.03	1.159AlaArg: 1.159 ± 0.03	2.448AlaSer: 2.448 ± 0.051	1.552AlaThr: 1.552 ± 0.034	1.669AlaVal: 1.669 ± 0.044	0.149AlaTrp: 0.149 ± 0.011	1.259AlaTyr: 1.259 ± 0.029	0.0AlaXaa: 0.0 ± 0.0
Cys	0.733CysAla: 0.733 ± 0.026	0.496CysCys: 0.496 ± 0.017	1.351CysAsp: 1.351 ± 0.029	1.452CysGlu: 1.452 ± 0.034	1.332CysPhe: 1.332 ± 0.03	0.905CysGly: 0.905 ± 0.03	0.282CysHis: 0.282 ± 0.013	1.702CysIle: 1.702 ± 0.036	1.862CysLys: 1.862 ± 0.035	1.883CysLeu: 1.883 ± 0.037	0.344CysMet: 0.344 ± 0.014	1.526CysAsn: 1.526 ± 0.036	0.433CysPro: 0.433 ± 0.023	0.469CysGln: 0.469 ± 0.02	0.666CysArg: 0.666 ± 0.022	1.479CysSer: 1.479 ± 0.033	0.843CysThr: 0.843 ± 0.027	1.111CysVal: 1.111 ± 0.025	0.091CysTrp: 0.091 ± 0.008	0.802CysTyr: 0.802 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	1.84AspAla: 1.84 ± 0.034	1.054AspCys: 1.054 ± 0.028	3.727AspAsp: 3.727 ± 0.061	4.555AspGlu: 4.555 ± 0.069	3.904AspPhe: 3.904 ± 0.056	1.757AspGly: 1.757 ± 0.043	0.971AspHis: 0.971 ± 0.025	5.616AspIle: 5.616 ± 0.08	5.611AspLys: 5.611 ± 0.079	5.2AspLeu: 5.2 ± 0.063	1.05AspMet: 1.05 ± 0.028	5.159AspAsn: 5.159 ± 0.1	1.286AspPro: 1.286 ± 0.034	1.797AspGln: 1.797 ± 0.046	1.668AspArg: 1.668 ± 0.044	4.667AspSer: 4.667 ± 0.064	2.56AspThr: 2.56 ± 0.042	2.86AspVal: 2.86 ± 0.044	0.237AspTrp: 0.237 ± 0.012	2.305AspTyr: 2.305 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	2.098GluAla: 2.098 ± 0.055	1.235GluCys: 1.235 ± 0.032	3.72GluAsp: 3.72 ± 0.061	5.272GluGlu: 5.272 ± 0.118	3.217GluPhe: 3.217 ± 0.056	1.552GluGly: 1.552 ± 0.046	1.102GluHis: 1.102 ± 0.027	7.012GluIle: 7.012 ± 0.081	9.213GluLys: 9.213 ± 0.108	4.653GluLeu: 4.653 ± 0.068	1.55GluMet: 1.55 ± 0.033	8.758GluAsn: 8.758 ± 0.113	1.211GluPro: 1.211 ± 0.028	1.872GluGln: 1.872 ± 0.04	2.207GluArg: 2.207 ± 0.04	4.95GluSer: 4.95 ± 0.072	3.348GluThr: 3.348 ± 0.046	2.55GluVal: 2.55 ± 0.042	0.26GluTrp: 0.26 ± 0.013	2.739GluTyr: 2.739 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	1.849PheAla: 1.849 ± 0.041	1.489PheCys: 1.489 ± 0.029	3.89PheAsp: 3.89 ± 0.051	3.67PheGlu: 3.67 ± 0.056	4.274PhePhe: 4.274 ± 0.068	1.98PheGly: 1.98 ± 0.043	1.048PheHis: 1.048 ± 0.025	5.034PheIle: 5.034 ± 0.073	4.856PheLys: 4.856 ± 0.065	6.319PheLeu: 6.319 ± 0.087	1.229PheMet: 1.229 ± 0.033	4.411PheAsn: 4.411 ± 0.061	1.279PhePro: 1.279 ± 0.028	1.658PheGln: 1.658 ± 0.034	1.814PheArg: 1.814 ± 0.041	4.792PheSer: 4.792 ± 0.065	2.58PheThr: 2.58 ± 0.046	3.167PheVal: 3.167 ± 0.055	0.35PheTrp: 0.35 ± 0.016	3.22PheTyr: 3.22 ± 0.048	0.0PheXaa: 0.0 ± 0.0
Gly	1.107GlyAla: 1.107 ± 0.043	0.682GlyCys: 0.682 ± 0.024	1.642GlyAsp: 1.642 ± 0.038	1.826GlyGlu: 1.826 ± 0.046	1.911GlyPhe: 1.911 ± 0.042	1.209GlyGly: 1.209 ± 0.052	0.593GlyHis: 0.593 ± 0.019	2.785GlyIle: 2.785 ± 0.05	3.103GlyLys: 3.103 ± 0.049	2.609GlyLeu: 2.609 ± 0.046	0.68GlyMet: 0.68 ± 0.026	2.632GlyAsn: 2.632 ± 0.05	0.591GlyPro: 0.591 ± 0.023	0.747GlyGln: 0.747 ± 0.022	1.092GlyArg: 1.092 ± 0.033	2.271GlySer: 2.271 ± 0.056	1.57GlyThr: 1.57 ± 0.039	1.533GlyVal: 1.533 ± 0.035	0.156GlyTrp: 0.156 ± 0.011	1.265GlyTyr: 1.265 ± 0.031	0.0GlyXaa: 0.0 ± 0.0
His	0.595HisAla: 0.595 ± 0.022	0.4HisCys: 0.4 ± 0.017	1.027HisAsp: 1.027 ± 0.028	1.362HisGlu: 1.362 ± 0.033	1.181HisPhe: 1.181 ± 0.028	0.658HisGly: 0.658 ± 0.023	0.438HisHis: 0.438 ± 0.017	1.786HisIle: 1.786 ± 0.037	1.959HisLys: 1.959 ± 0.035	1.75HisLeu: 1.75 ± 0.033	0.357HisMet: 0.357 ± 0.015	1.696HisAsn: 1.696 ± 0.038	0.555HisPro: 0.555 ± 0.02	0.637HisGln: 0.637 ± 0.025	0.708HisArg: 0.708 ± 0.022	1.487HisSer: 1.487 ± 0.032	1.025HisThr: 1.025 ± 0.028	0.898HisVal: 0.898 ± 0.025	0.08HisTrp: 0.08 ± 0.007	0.735HisTyr: 0.735 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	2.917IleAla: 2.917 ± 0.057	2.027IleCys: 2.027 ± 0.041	6.027IleAsp: 6.027 ± 0.078	6.307IleGlu: 6.307 ± 0.075	6.083IlePhe: 6.083 ± 0.079	2.739IleGly: 2.739 ± 0.047	1.817IleHis: 1.817 ± 0.039	7.525IleIle: 7.525 ± 0.091	9.164IleLys: 9.164 ± 0.095	8.919IleLeu: 8.919 ± 0.095	1.594IleMet: 1.594 ± 0.035	8.492IleAsn: 8.492 ± 0.132	2.377IlePro: 2.377 ± 0.043	3.04IleGln: 3.04 ± 0.051	2.791IleArg: 2.791 ± 0.057	7.644IleSer: 7.644 ± 0.09	4.163IleThr: 4.163 ± 0.063	4.26IleVal: 4.26 ± 0.064	0.381IleTrp: 0.381 ± 0.016	4.286IleTyr: 4.286 ± 0.058	0.0IleXaa: 0.0 ± 0.0
Lys	2.798LysAla: 2.798 ± 0.054	1.805LysCys: 1.805 ± 0.034	5.494LysAsp: 5.494 ± 0.079	7.101LysGlu: 7.101 ± 0.103	4.803LysPhe: 4.803 ± 0.064	2.537LysGly: 2.537 ± 0.046	2.147LysHis: 2.147 ± 0.037	11.187LysIle: 11.187 ± 0.104	12.826LysLys: 12.826 ± 0.134	7.618LysLeu: 7.618 ± 0.077	2.53LysMet: 2.53 ± 0.043	13.129LysAsn: 13.129 ± 0.142	2.417LysPro: 2.417 ± 0.052	3.182LysGln: 3.182 ± 0.044	3.636LysArg: 3.636 ± 0.051	7.682LysSer: 7.682 ± 0.09	5.855LysThr: 5.855 ± 0.065	3.604LysVal: 3.604 ± 0.056	0.386LysTrp: 0.386 ± 0.017	4.718LysTyr: 4.718 ± 0.059	0.0LysXaa: 0.0 ± 0.0
Leu	2.795LeuAla: 2.795 ± 0.055	1.93LeuCys: 1.93 ± 0.039	4.743LeuAsp: 4.743 ± 0.062	5.803LeuGlu: 5.803 ± 0.08	5.36LeuPhe: 5.36 ± 0.067	2.488LeuGly: 2.488 ± 0.05	1.85LeuHis: 1.85 ± 0.039	7.311LeuIle: 7.311 ± 0.08	9.577LeuLys: 9.577 ± 0.102	7.847LeuLeu: 7.847 ± 0.096	1.731LeuMet: 1.731 ± 0.04	7.851LeuAsn: 7.851 ± 0.096	2.204LeuPro: 2.204 ± 0.041	3.157LeuGln: 3.157 ± 0.056	3.163LeuArg: 3.163 ± 0.055	6.71LeuSer: 6.71 ± 0.078	3.672LeuThr: 3.672 ± 0.065	3.772LeuVal: 3.772 ± 0.061	0.434LeuTrp: 0.434 ± 0.016	3.932LeuTyr: 3.932 ± 0.065	0.0LeuXaa: 0.0 ± 0.0
Met	0.712MetAla: 0.712 ± 0.023	0.477MetCys: 0.477 ± 0.015	0.885MetAsp: 0.885 ± 0.024	1.118MetGlu: 1.118 ± 0.029	1.225MetPhe: 1.225 ± 0.032	0.578MetGly: 0.578 ± 0.02	0.542MetHis: 0.542 ± 0.019	1.724MetIle: 1.724 ± 0.032	2.193MetLys: 2.193 ± 0.041	1.949MetLeu: 1.949 ± 0.037	0.458MetMet: 0.458 ± 0.019	1.74MetAsn: 1.74 ± 0.041	0.572MetPro: 0.572 ± 0.024	0.833MetGln: 0.833 ± 0.027	0.795MetArg: 0.795 ± 0.023	1.39MetSer: 1.39 ± 0.028	0.785MetThr: 0.785 ± 0.022	0.929MetVal: 0.929 ± 0.025	0.123MetTrp: 0.123 ± 0.009	0.816MetTyr: 0.816 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	3.046AsnAla: 3.046 ± 0.065	1.615AsnCys: 1.615 ± 0.037	5.725AsnAsp: 5.725 ± 0.081	6.477AsnGlu: 6.477 ± 0.082	5.374AsnPhe: 5.374 ± 0.074	2.661AsnGly: 2.661 ± 0.051	1.798AsnHis: 1.798 ± 0.04	10.641AsnIle: 10.641 ± 0.167	9.253AsnLys: 9.253 ± 0.114	8.489AsnLeu: 8.489 ± 0.084	1.97AsnMet: 1.97 ± 0.037	11.613AsnAsn: 11.613 ± 0.332	2.313AsnPro: 2.313 ± 0.069	3.312AsnGln: 3.312 ± 0.067	2.804AsnArg: 2.804 ± 0.047	8.378AsnSer: 8.378 ± 0.152	6.112AsnThr: 6.112 ± 0.137	4.402AsnVal: 4.402 ± 0.068	0.325AsnTrp: 0.325 ± 0.014	3.682AsnTyr: 3.682 ± 0.058	0.001AsnXaa: 0.001 ± 0.001
Pro	0.869ProAla: 0.869 ± 0.032	0.429ProCys: 0.429 ± 0.021	1.219ProAsp: 1.219 ± 0.032	1.775ProGlu: 1.775 ± 0.048	1.472ProPhe: 1.472 ± 0.037	0.812ProGly: 0.812 ± 0.039	0.49ProHis: 0.49 ± 0.019	2.091ProIle: 2.091 ± 0.042	2.287ProLys: 2.287 ± 0.043	2.033ProLeu: 2.033 ± 0.047	0.418ProMet: 0.418 ± 0.016	2.047ProAsn: 2.047 ± 0.057	0.777ProPro: 0.777 ± 0.08	0.973ProGln: 0.973 ± 0.033	0.776ProArg: 0.776 ± 0.023	1.869ProSer: 1.869 ± 0.058	1.27ProThr: 1.27 ± 0.034	1.292ProVal: 1.292 ± 0.037	0.091ProTrp: 0.091 ± 0.008	0.881ProTyr: 0.881 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	0.961GlnAla: 0.961 ± 0.034	0.519GlnCys: 0.519 ± 0.017	1.416GlnAsp: 1.416 ± 0.031	2.239GlnGlu: 2.239 ± 0.04	1.481GlnPhe: 1.481 ± 0.036	0.736GlnGly: 0.736 ± 0.026	0.616GlnHis: 0.616 ± 0.022	3.176GlnIle: 3.176 ± 0.047	4.263GlnLys: 4.263 ± 0.063	2.218GlnLeu: 2.218 ± 0.042	0.655GlnMet: 0.655 ± 0.022	4.105GlnAsn: 4.105 ± 0.073	0.827GlnPro: 0.827 ± 0.032	1.249GlnGln: 1.249 ± 0.035	1.134GlnArg: 1.134 ± 0.03	2.449GlnSer: 2.449 ± 0.046	1.67GlnThr: 1.67 ± 0.034	1.134GlnVal: 1.134 ± 0.032	0.118GlnTrp: 0.118 ± 0.009	1.271GlnTyr: 1.271 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	0.96ArgAla: 0.96 ± 0.027	0.627ArgCys: 0.627 ± 0.024	1.63ArgAsp: 1.63 ± 0.042	2.106ArgGlu: 2.106 ± 0.045	1.794ArgPhe: 1.794 ± 0.035	1.013ArgGly: 1.013 ± 0.034	0.62ArgHis: 0.62 ± 0.02	3.096ArgIle: 3.096 ± 0.049	4.249ArgLys: 4.249 ± 0.056	2.606ArgLeu: 2.606 ± 0.047	0.79ArgMet: 0.79 ± 0.024	3.346ArgAsn: 3.346 ± 0.049	0.747ArgPro: 0.747 ± 0.026	0.95ArgGln: 0.95 ± 0.027	1.625ArgArg: 1.625 ± 0.042	2.333ArgSer: 2.333 ± 0.044	1.47ArgThr: 1.47 ± 0.035	1.412ArgVal: 1.412 ± 0.039	0.164ArgTrp: 0.164 ± 0.01	1.391ArgTyr: 1.391 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	2.767SerAla: 2.767 ± 0.052	1.395SerCys: 1.395 ± 0.029	4.909SerAsp: 4.909 ± 0.056	5.503SerGlu: 5.503 ± 0.06	4.33SerPhe: 4.33 ± 0.06	2.49SerGly: 2.49 ± 0.045	1.518SerHis: 1.518 ± 0.033	6.669SerIle: 6.669 ± 0.08	7.956SerLys: 7.956 ± 0.099	6.496SerLeu: 6.496 ± 0.068	1.378SerMet: 1.378 ± 0.03	8.266SerAsn: 8.266 ± 0.162	1.77SerPro: 1.77 ± 0.053	2.649SerGln: 2.649 ± 0.049	2.393SerArg: 2.393 ± 0.041	7.396SerSer: 7.396 ± 0.133	4.129SerThr: 4.129 ± 0.07	3.566SerVal: 3.566 ± 0.055	0.321SerTrp: 0.321 ± 0.016	3.008SerTyr: 3.008 ± 0.046	0.0SerXaa: 0.0 ± 0.0
Thr	1.717ThrAla: 1.717 ± 0.037	0.877ThrCys: 0.877 ± 0.027	2.906ThrAsp: 2.906 ± 0.05	3.561ThrGlu: 3.561 ± 0.058	2.722ThrPhe: 2.722 ± 0.043	1.615ThrGly: 1.615 ± 0.032	1.036ThrHis: 1.036 ± 0.029	4.394ThrIle: 4.394 ± 0.057	5.032ThrLys: 5.032 ± 0.061	3.998ThrLeu: 3.998 ± 0.053	0.812ThrMet: 0.812 ± 0.025	4.911ThrAsn: 4.911 ± 0.097	1.425ThrPro: 1.425 ± 0.049	1.734ThrGln: 1.734 ± 0.037	1.531ThrArg: 1.531 ± 0.032	4.037ThrSer: 4.037 ± 0.072	2.793ThrThr: 2.793 ± 0.054	2.365ThrVal: 2.365 ± 0.048	0.193ThrTrp: 0.193 ± 0.012	1.838ThrTyr: 1.838 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	1.587ValAla: 1.587 ± 0.04	1.102ValCys: 1.102 ± 0.033	2.976ValAsp: 2.976 ± 0.046	3.073ValGlu: 3.073 ± 0.051	3.339ValPhe: 3.339 ± 0.046	1.52ValGly: 1.52 ± 0.039	0.859ValHis: 0.859 ± 0.022	3.581ValIle: 3.581 ± 0.051	4.258ValLys: 4.258 ± 0.062	4.329ValLeu: 4.329 ± 0.056	0.738ValMet: 0.738 ± 0.02	3.562ValAsn: 3.562 ± 0.053	1.282ValPro: 1.282 ± 0.033	1.395ValGln: 1.395 ± 0.034	1.381ValArg: 1.381 ± 0.032	3.466ValSer: 3.466 ± 0.056	1.891ValThr: 1.891 ± 0.043	2.451ValVal: 2.451 ± 0.05	0.223ValTrp: 0.223 ± 0.011	2.084ValTyr: 2.084 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	0.164TrpAla: 0.164 ± 0.012	0.103TrpCys: 0.103 ± 0.009	0.248TrpAsp: 0.248 ± 0.015	0.231TrpGlu: 0.231 ± 0.013	0.322TrpPhe: 0.322 ± 0.015	0.171TrpGly: 0.171 ± 0.014	0.098TrpHis: 0.098 ± 0.007	0.369TrpIle: 0.369 ± 0.018	0.39TrpLys: 0.39 ± 0.018	0.421TrpLeu: 0.421 ± 0.016	0.115TrpMet: 0.115 ± 0.009	0.31TrpAsn: 0.31 ± 0.014	0.111TrpPro: 0.111 ± 0.009	0.131TrpGln: 0.131 ± 0.009	0.171TrpArg: 0.171 ± 0.011	0.32TrpSer: 0.32 ± 0.015	0.182TrpThr: 0.182 ± 0.012	0.229TrpVal: 0.229 ± 0.012	0.023TrpTrp: 0.023 ± 0.004	0.205TrpTyr: 0.205 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.378TyrAla: 1.378 ± 0.031	0.906TyrCys: 0.906 ± 0.027	2.476TyrAsp: 2.476 ± 0.044	2.766TyrGlu: 2.766 ± 0.046	2.937TyrPhe: 2.937 ± 0.057	1.392TyrGly: 1.392 ± 0.032	0.87TyrHis: 0.87 ± 0.025	3.913TyrIle: 3.913 ± 0.063	4.254TyrLys: 4.254 ± 0.066	3.992TyrLeu: 3.992 ± 0.058	0.821TyrMet: 0.821 ± 0.024	3.845TyrAsn: 3.845 ± 0.076	0.911TyrPro: 0.911 ± 0.027	1.29TyrGln: 1.29 ± 0.033	1.412TyrArg: 1.412 ± 0.036	3.14TyrSer: 3.14 ± 0.051	2.026TyrThr: 2.026 ± 0.035	1.897TyrVal: 1.897 ± 0.034	0.21TyrTrp: 0.21 ± 0.01	1.798TyrTyr: 1.798 ± 0.041	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4189 proteins (1478472 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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