Alteribacillus persepolensis
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3572 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G8EIZ4|A0A1G8EIZ4_9BACI Acyl-CoA dehydrogenase OS=Alteribacillus persepolensis OX=568899 GN=SAMN05192534_109117 PE=3 SV=1
MM1 pKa = 8.08 DD2 pKa = 6.34 NILKK6 pKa = 9.83 MSFAALFSAVVLAACGGNDD25 pKa = 3.27 EE26 pKa = 4.67 TTPDD30 pKa = 3.5 SNANTEE36 pKa = 3.67 NDD38 pKa = 3.35 AAEE41 pKa = 3.96 NTEE44 pKa = 4.26 TNEE47 pKa = 3.9 EE48 pKa = 4.12 APSEE52 pKa = 4.21 EE53 pKa = 4.68 EE54 pKa = 3.84 SDD56 pKa = 3.63 SMEE59 pKa = 4.21 EE60 pKa = 3.96 MDD62 pKa = 5.25 HH63 pKa = 8.25 SRR65 pKa = 11.84 MDD67 pKa = 3.32 HH68 pKa = 6.11 SSSGEE73 pKa = 4.08 VPEE76 pKa = 4.77 EE77 pKa = 4.12 LADD80 pKa = 4.08 AQNPTYY86 pKa = 10.03 PVGSEE91 pKa = 3.52 AVMSANHH98 pKa = 5.4 MRR100 pKa = 11.84 GMDD103 pKa = 3.63 GAVAAIEE110 pKa = 4.23 GAYY113 pKa = 8.44 DD114 pKa = 3.27 TTVYY118 pKa = 9.95 MVTYY122 pKa = 8.69 TPAAGEE128 pKa = 4.26 EE129 pKa = 4.24 KK130 pKa = 10.83 VEE132 pKa = 3.86 DD133 pKa = 4.25 HH134 pKa = 6.5 KK135 pKa = 11.06 WVIHH139 pKa = 5.63 EE140 pKa = 4.55 EE141 pKa = 3.94 IEE143 pKa = 4.34 NAGEE147 pKa = 3.88 QPYY150 pKa = 10.03 EE151 pKa = 4.06 QGDD154 pKa = 4.11 EE155 pKa = 4.51 VILEE159 pKa = 4.28 ADD161 pKa = 3.62 HH162 pKa = 6.43 MEE164 pKa = 4.42 GMDD167 pKa = 3.49 GAAAVIDD174 pKa = 3.93 AAEE177 pKa = 4.34 DD178 pKa = 3.52 TTVYY182 pKa = 9.23 MVSYY186 pKa = 10.52 TDD188 pKa = 3.43 MEE190 pKa = 4.41 TGEE193 pKa = 4.28 EE194 pKa = 4.35 VEE196 pKa = 3.93 NHH198 pKa = 4.77 KK199 pKa = 10.2 WVTEE203 pKa = 4.16 DD204 pKa = 3.13 EE205 pKa = 4.48 LSPVEE210 pKa = 4.06
Molecular weight: 22.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.732
IPC_protein 3.706
Toseland 3.528
ProMoST 3.821
Dawson 3.668
Bjellqvist 3.846
Wikipedia 3.541
Rodwell 3.541
Grimsley 3.439
Solomon 3.656
Lehninger 3.605
Nozaki 3.783
DTASelect 3.923
Thurlkill 3.554
EMBOSS 3.567
Sillero 3.821
Patrickios 1.125
IPC_peptide 3.656
IPC2_peptide 3.808
IPC2.peptide.svr19 3.735
Protein with the highest isoelectric point:
>tr|A0A1G8J6F9|A0A1G8J6F9_9BACI Uncharacterized protein OS=Alteribacillus persepolensis OX=568899 GN=SAMN05192534_12916 PE=4 SV=1
MM1 pKa = 7.69 GKK3 pKa = 7.88 PTYY6 pKa = 9.67 TPNNRR11 pKa = 11.84 KK12 pKa = 9.21 RR13 pKa = 11.84 KK14 pKa = 8.37 KK15 pKa = 8.69 VHH17 pKa = 5.46 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MSTKK26 pKa = 10.26 NGRR29 pKa = 11.84 HH30 pKa = 3.85 VLKK33 pKa = 10.52 NRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 8.66 GRR40 pKa = 11.84 KK41 pKa = 8.69 VLSAA45 pKa = 4.05
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.391
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.384
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.106
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.993
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3572
0
3572
1005916
24
1691
281.6
31.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.834 ± 0.044
0.747 ± 0.013
5.397 ± 0.04
7.945 ± 0.057
4.243 ± 0.033
6.962 ± 0.047
2.464 ± 0.025
6.791 ± 0.037
6.191 ± 0.044
9.204 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.936 ± 0.019
3.989 ± 0.025
3.804 ± 0.027
4.092 ± 0.029
4.282 ± 0.029
5.836 ± 0.031
5.527 ± 0.027
7.248 ± 0.038
1.077 ± 0.015
3.431 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here