Hubei sobemo-like virus 23

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEQ3|A0A1L3KEQ3_9VIRU C2H2-type domain-containing protein OS=Hubei sobemo-like virus 23 OX=1923209 PE=4 SV=1
MM1 pKa = 7.61ASDD4 pKa = 4.02FTWPLLGGDD13 pKa = 3.71AEE15 pKa = 4.42LRR17 pKa = 11.84SLLTHH22 pKa = 5.46VQMSEE27 pKa = 3.76GLRR30 pKa = 11.84EE31 pKa = 4.77DD32 pKa = 4.58GPSHH36 pKa = 6.42QIKK39 pKa = 9.16TKK41 pKa = 10.58LLDD44 pKa = 3.3IAEE47 pKa = 4.84DD48 pKa = 3.58MFKK51 pKa = 10.16PARR54 pKa = 11.84WRR56 pKa = 11.84IPDD59 pKa = 3.93DD60 pKa = 3.57FLSFNHH66 pKa = 6.07YY67 pKa = 9.38MRR69 pKa = 11.84TLNQLVWTSSPGLPYY84 pKa = 9.93MRR86 pKa = 11.84TYY88 pKa = 10.39PNNGGMFGVVDD99 pKa = 4.28GVPNPEE105 pKa = 3.88KK106 pKa = 10.5CRR108 pKa = 11.84YY109 pKa = 6.63WWTIVQQKK117 pKa = 9.68IRR119 pKa = 11.84DD120 pKa = 3.67QFSDD124 pKa = 3.82YY125 pKa = 10.71IRR127 pKa = 11.84VFIKK131 pKa = 10.33QEE133 pKa = 4.12PITRR137 pKa = 11.84KK138 pKa = 9.98KK139 pKa = 10.57FEE141 pKa = 4.07QNRR144 pKa = 11.84FRR146 pKa = 11.84LIFSVSVLDD155 pKa = 4.56QIIDD159 pKa = 3.35HH160 pKa = 6.2MLFGDD165 pKa = 4.09FNDD168 pKa = 3.94LVIEE172 pKa = 3.99NWYY175 pKa = 10.19NLPTKK180 pKa = 10.53VGWSPLGGGWKK191 pKa = 9.93IIPKK195 pKa = 9.31SKK197 pKa = 10.2CHH199 pKa = 6.93AIDD202 pKa = 3.1KK203 pKa = 10.08RR204 pKa = 11.84AWDD207 pKa = 3.59WTVKK211 pKa = 8.5MWLIEE216 pKa = 4.19LEE218 pKa = 3.87FMLRR222 pKa = 11.84VRR224 pKa = 11.84LCDD227 pKa = 3.39NMNPLWRR234 pKa = 11.84ALAFWRR240 pKa = 11.84YY241 pKa = 9.67KK242 pKa = 10.74SLFMDD247 pKa = 4.0AKK249 pKa = 9.99FVNSGGLVFKK259 pKa = 10.8QKK261 pKa = 10.79FIGVMKK267 pKa = 9.94SGCVNTIITNSIAQILLHH285 pKa = 6.78LRR287 pKa = 11.84ICLEE291 pKa = 4.32LGLDD295 pKa = 3.52ISWIFSLGDD304 pKa = 3.33DD305 pKa = 4.12TLQEE309 pKa = 4.29LLSDD313 pKa = 3.74PRR315 pKa = 11.84YY316 pKa = 10.6LEE318 pKa = 4.05LLKK321 pKa = 10.77QFSLVKK327 pKa = 9.94EE328 pKa = 3.95CVIANEE334 pKa = 4.15FAGMRR339 pKa = 11.84YY340 pKa = 9.89AGGVVDD346 pKa = 4.78PLYY349 pKa = 10.61KK350 pKa = 10.42GKK352 pKa = 9.53HH353 pKa = 5.3AFNILHH359 pKa = 5.98VNPKK363 pKa = 9.7FGQEE367 pKa = 3.44IAEE370 pKa = 4.88GYY372 pKa = 9.71SILYY376 pKa = 9.12HH377 pKa = 6.33RR378 pKa = 11.84SPRR381 pKa = 11.84GSFIKK386 pKa = 10.54QIFKK390 pKa = 11.24DD391 pKa = 3.47MGYY394 pKa = 8.87TIPSDD399 pKa = 4.1DD400 pKa = 4.7ALDD403 pKa = 4.99LIWDD407 pKa = 3.94GEE409 pKa = 4.34EE410 pKa = 3.76

Molecular weight: 47.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEQ3|A0A1L3KEQ3_9VIRU C2H2-type domain-containing protein OS=Hubei sobemo-like virus 23 OX=1923209 PE=4 SV=1
MM1 pKa = 7.78FITEE5 pKa = 4.75FVSKK9 pKa = 10.3ILSNIRR15 pKa = 11.84NAIVPFVMAYY25 pKa = 8.98ILYY28 pKa = 9.6YY29 pKa = 10.89FMDD32 pKa = 3.96RR33 pKa = 11.84KK34 pKa = 10.46EE35 pKa = 4.32EE36 pKa = 4.24YY37 pKa = 9.79IDD39 pKa = 3.72TYY41 pKa = 11.45DD42 pKa = 3.72AVIDD46 pKa = 3.92SVRR49 pKa = 11.84PKK51 pKa = 10.49IVVQPSAEE59 pKa = 3.82FDD61 pKa = 3.7DD62 pKa = 6.37FIDD65 pKa = 4.12EE66 pKa = 4.55FLSKK70 pKa = 10.58AWTHH74 pKa = 4.38IRR76 pKa = 11.84RR77 pKa = 11.84YY78 pKa = 10.25VSLKK82 pKa = 9.13GHH84 pKa = 6.99RR85 pKa = 11.84IWYY88 pKa = 9.12LIVKK92 pKa = 9.06VLLVVLTVYY101 pKa = 11.06LLYY104 pKa = 10.8KK105 pKa = 10.25GIRR108 pKa = 11.84KK109 pKa = 8.94LFSFIKK115 pKa = 9.8IKK117 pKa = 10.2MYY119 pKa = 10.41DD120 pKa = 3.25FCGVKK125 pKa = 10.2DD126 pKa = 4.12FRR128 pKa = 11.84GEE130 pKa = 3.7AMKK133 pKa = 10.71AGSSFGKK140 pKa = 9.03GTVPDD145 pKa = 3.97YY146 pKa = 10.59QVEE149 pKa = 4.11IHH151 pKa = 7.02RR152 pKa = 11.84SGTFMFSFSGYY163 pKa = 7.41GLRR166 pKa = 11.84YY167 pKa = 9.59GDD169 pKa = 3.7VLVVPTHH176 pKa = 6.24VIGNSRR182 pKa = 11.84DD183 pKa = 3.39FKK185 pKa = 11.4LVGKK189 pKa = 8.26KK190 pKa = 7.3TAYY193 pKa = 9.59ISLDD197 pKa = 3.64FVASTLMNDD206 pKa = 3.03VSYY209 pKa = 11.49VRR211 pKa = 11.84LGTDD215 pKa = 2.49VWSALGTKK223 pKa = 8.34SCKK226 pKa = 9.96YY227 pKa = 9.82IKK229 pKa = 10.08PNYY232 pKa = 7.78TPQVVTIVGKK242 pKa = 10.47QGATSGILRR251 pKa = 11.84KK252 pKa = 9.39SEE254 pKa = 4.15CEE256 pKa = 3.78FMMEE260 pKa = 4.42YY261 pKa = 10.16EE262 pKa = 4.57GSTIPGMSGAAYY274 pKa = 7.62FTQAGPLAMHH284 pKa = 6.72SGVVLGRR291 pKa = 11.84TNVGYY296 pKa = 8.64TINAICMEE304 pKa = 4.43VDD306 pKa = 3.28TTFPPSVVGEE316 pKa = 4.01GRR318 pKa = 11.84IADD321 pKa = 3.66ANEE324 pKa = 4.23DD325 pKa = 3.95YY326 pKa = 10.9EE327 pKa = 4.68FSGRR331 pKa = 11.84RR332 pKa = 11.84KK333 pKa = 8.68KK334 pKa = 10.64AAWRR338 pKa = 11.84EE339 pKa = 4.24SKK341 pKa = 10.88VKK343 pKa = 10.82SSWDD347 pKa = 3.27NSSIKK352 pKa = 10.48ARR354 pKa = 11.84LGFADD359 pKa = 3.85VSDD362 pKa = 3.68EE363 pKa = 4.66RR364 pKa = 11.84IAEE367 pKa = 4.19LEE369 pKa = 4.05RR370 pKa = 11.84FANADD375 pKa = 3.85DD376 pKa = 4.31AWTKK380 pKa = 10.61DD381 pKa = 2.88MKK383 pKa = 10.25MDD385 pKa = 3.89YY386 pKa = 10.17NVKK389 pKa = 10.66LNFEE393 pKa = 4.69GEE395 pKa = 4.13NFDD398 pKa = 3.7PSKK401 pKa = 11.03KK402 pKa = 9.96IFATDD407 pKa = 3.0GHH409 pKa = 5.79GKK411 pKa = 9.8KK412 pKa = 10.57VIILGTSDD420 pKa = 4.42LNFSGFKK427 pKa = 9.9PYY429 pKa = 10.74SATASGQAPDD439 pKa = 4.0EE440 pKa = 4.05PQIRR444 pKa = 11.84IGAVEE449 pKa = 4.33PSLGDD454 pKa = 2.97IVRR457 pKa = 11.84DD458 pKa = 3.38HH459 pKa = 6.69EE460 pKa = 4.26RR461 pKa = 11.84RR462 pKa = 11.84IVALEE467 pKa = 3.83AKK469 pKa = 9.99IKK471 pKa = 10.31SLQSKK476 pKa = 9.29PPGYY480 pKa = 10.87VCGHH484 pKa = 5.92CAKK487 pKa = 10.38PFDD490 pKa = 4.48LEE492 pKa = 4.07EE493 pKa = 5.34SLMQHH498 pKa = 6.02VKK500 pKa = 10.21SKK502 pKa = 10.36HH503 pKa = 5.29IKK505 pKa = 10.35GEE507 pKa = 4.07SAYY510 pKa = 9.65KK511 pKa = 10.6DD512 pKa = 3.51HH513 pKa = 7.73FDD515 pKa = 6.35DD516 pKa = 5.78ILNKK520 pKa = 10.26DD521 pKa = 3.13KK522 pKa = 10.64KK523 pKa = 9.33TFLEE527 pKa = 4.3QKK529 pKa = 10.27PSPTKK534 pKa = 9.88NAPNSRR540 pKa = 11.84STSRR544 pKa = 11.84SKK546 pKa = 10.68EE547 pKa = 3.73KK548 pKa = 10.46KK549 pKa = 9.06VPSTSRR555 pKa = 11.84EE556 pKa = 4.06GNRR559 pKa = 11.84SS560 pKa = 3.13

Molecular weight: 62.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

970

410

560

485.0

55.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.361 ± 0.909

1.237 ± 0.141

6.598 ± 0.144

5.155 ± 0.324

5.773 ± 0.202

7.216 ± 0.089

2.062 ± 0.083

7.113 ± 0.431

7.835 ± 0.93

8.041 ± 1.828

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.99 ± 0.265

3.608 ± 0.335

4.33 ± 0.19

2.577 ± 0.674

5.258 ± 0.067

7.629 ± 1.106

4.33 ± 0.57

6.804 ± 0.896

2.165 ± 0.93

3.918 ± 0.465
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski