Jejubacter calystegiae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Jejubacter

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4678 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8YCV7|A0A4P8YCV7_9ENTR Transketolase OS=Jejubacter calystegiae OX=2579935 GN=tkt PE=3 SV=1
MM1 pKa = 6.63VTFLGFKK8 pKa = 10.16EE9 pKa = 4.28LLNSYY14 pKa = 7.95KK15 pKa = 10.08TLPDD19 pKa = 3.69VGWLYY24 pKa = 11.34VDD26 pKa = 3.88EE27 pKa = 4.6SFNLGSKK34 pKa = 10.66DD35 pKa = 3.78DD36 pKa = 3.99IVNGRR41 pKa = 11.84YY42 pKa = 9.29YY43 pKa = 10.75LAEE46 pKa = 4.36NEE48 pKa = 4.06DD49 pKa = 3.69EE50 pKa = 4.79EE51 pKa = 4.61MDD53 pKa = 3.76FEE55 pKa = 4.23EE56 pKa = 5.45SYY58 pKa = 9.85GTFLEE63 pKa = 4.29SPIFKK68 pKa = 10.43AIVDD72 pKa = 3.84NTLDD76 pKa = 3.39HH77 pKa = 6.97HH78 pKa = 6.97PDD80 pKa = 3.05SGEE83 pKa = 3.72IDD85 pKa = 3.86FLNATLYY92 pKa = 10.91YY93 pKa = 10.63LEE95 pKa = 5.47NDD97 pKa = 4.3DD98 pKa = 6.26FLDD101 pKa = 3.62

Molecular weight: 11.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8YF50|A0A4P8YF50_9ENTR Outer membrane channel protein TolC OS=Jejubacter calystegiae OX=2579935 GN=tolC PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSSKK47 pKa = 11.16

Molecular weight: 5.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4678

0

4678

1506925

14

6399

322.1

35.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.99 ± 0.05

1.128 ± 0.014

5.302 ± 0.059

5.59 ± 0.038

3.683 ± 0.033

7.902 ± 0.043

2.243 ± 0.017

5.518 ± 0.034

3.585 ± 0.032

10.883 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.732 ± 0.023

3.484 ± 0.027

4.64 ± 0.028

4.479 ± 0.029

6.281 ± 0.047

6.086 ± 0.045

5.2 ± 0.046

6.969 ± 0.033

1.633 ± 0.016

2.672 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski