Maribacter sp. T28
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3578 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B7Z8K2|A0A1B7Z8K2_9FLAO Rhomboid family intramembrane serine protease OS=Maribacter sp. T28 OX=1836467 GN=A9200_04285 PE=4 SV=1
MM1 pKa = 7.48 RR2 pKa = 11.84 TLLSCILFLCITLSYY17 pKa = 9.77 SQSEE21 pKa = 5.41 FITTWKK27 pKa = 8.84 TDD29 pKa = 3.68 NPGSTAPNSIVIPTYY44 pKa = 10.09 PGEE47 pKa = 4.4 TYY49 pKa = 10.37 DD50 pKa = 4.06 YY51 pKa = 10.4 TVDD54 pKa = 3.32 WGDD57 pKa = 3.46 GTIEE61 pKa = 5.27 SNLTDD66 pKa = 3.94 NAIHH70 pKa = 6.77 TYY72 pKa = 7.79 ATPGIYY78 pKa = 9.97 SVAISGTFPRR88 pKa = 11.84 IYY90 pKa = 10.41 FNNSGDD96 pKa = 3.78 KK97 pKa = 10.7 EE98 pKa = 4.14 KK99 pKa = 11.03 LILVNQWGSTNWSSMEE115 pKa = 4.11 KK116 pKa = 10.55 AFSGCINLDD125 pKa = 3.41 VVATDD130 pKa = 3.5 TPNFLGVNDD139 pKa = 4.94 LSYY142 pKa = 11.32 LFNGCTSLVGNNSFSNWNTSNVNNMRR168 pKa = 11.84 AVFTDD173 pKa = 3.24 TDD175 pKa = 4.21 SFNQDD180 pKa = 2.22 IGSWDD185 pKa = 3.41 TSEE188 pKa = 4.46 VINMAEE194 pKa = 3.96 LFKK197 pKa = 11.04 NSKK200 pKa = 10.15 SFNQDD205 pKa = 1.97 IGNWNISSVTNLAATFNGAILFNQNIGNWDD235 pKa = 3.43 VSSVEE240 pKa = 4.03 VFLGTFANAQNFNQNIGDD258 pKa = 3.92 WDD260 pKa = 3.75 VSAATDD266 pKa = 3.59 MAGMFSNAISFNQDD280 pKa = 1.79 IGRR283 pKa = 11.84 WDD285 pKa = 3.36 VSKK288 pKa = 10.81 VKK290 pKa = 10.72 RR291 pKa = 11.84 MINMFNFAISFNQNIGFWNVSNVTSMNSMFYY322 pKa = 10.4 NAQNFNQTLSYY333 pKa = 9.95 WNVSNVEE340 pKa = 4.1 NMNSMFDD347 pKa = 3.26 RR348 pKa = 11.84 ASNFDD353 pKa = 3.41 QNLGYY358 pKa = 10.4 WNISKK363 pKa = 8.23 VTDD366 pKa = 3.82 LEE368 pKa = 4.59 YY369 pKa = 10.51 MFSNAGLSTYY379 pKa = 10.92 NYY381 pKa = 10.05 DD382 pKa = 3.22 ATLNGWAQLPILQSNLTFDD401 pKa = 4.18 AGNSRR406 pKa = 11.84 YY407 pKa = 9.82 CFSEE411 pKa = 3.86 TSRR414 pKa = 11.84 NKK416 pKa = 10.42 LLNDD420 pKa = 4.15 LSWIINDD427 pKa = 3.34 AGKK430 pKa = 9.95 NLDD433 pKa = 4.24 CKK435 pKa = 10.86 PEE437 pKa = 4.15 LFTTLWKK444 pKa = 9.45 TDD446 pKa = 3.32 NSGSSGDD453 pKa = 3.77 NQITIPTFSGEE464 pKa = 4.02 AYY466 pKa = 10.38 NYY468 pKa = 7.79 TVYY471 pKa = 10.19 WGDD474 pKa = 3.43 GTVSSNVTGNITHH487 pKa = 7.39 TYY489 pKa = 8.38 YY490 pKa = 10.81 EE491 pKa = 4.27 PGAYY495 pKa = 8.8 QVSIEE500 pKa = 4.17 GAFPRR505 pKa = 11.84 IYY507 pKa = 10.62 FNEE510 pKa = 4.03 TGDD513 pKa = 3.53 RR514 pKa = 11.84 EE515 pKa = 4.32 KK516 pKa = 10.67 ILEE519 pKa = 4.1 VKK521 pKa = 9.8 QWGDD525 pKa = 3.33 VSWNSMEE532 pKa = 4.38 SAFAGCSNLSVAANDD547 pKa = 3.65 VPNLSNTTSLKK558 pKa = 10.73 RR559 pKa = 11.84 MFLNCGYY566 pKa = 10.73 AFDD569 pKa = 4.21 FTRR572 pKa = 11.84 SRR574 pKa = 11.84 EE575 pKa = 3.79 YY576 pKa = 11.13 RR577 pKa = 11.84 NFNGIEE583 pKa = 4.17 NFNSWDD589 pKa = 3.34 VSTITDD595 pKa = 3.66 MSNMFDD601 pKa = 3.42 KK602 pKa = 11.22 SSFNQDD608 pKa = 1.91 ISQWNVSNVTDD619 pKa = 3.79 ISYY622 pKa = 10.48 IFYY625 pKa = 10.4 SSPFNFEE632 pKa = 3.47 VSNWDD637 pKa = 3.17 VSSVVNMQGVFGSSNFNKK655 pKa = 10.13 SVSSWDD661 pKa = 3.37 VSNVTNMDD669 pKa = 3.37 YY670 pKa = 10.66 MFNSTNFDD678 pKa = 3.13 QDD680 pKa = 2.88 ISSWNVSNVTSMRR693 pKa = 11.84 NMLSQGSFNQDD704 pKa = 2.0 ISSWNVSNVLDD715 pKa = 3.85 MSNIFDD721 pKa = 5.23 DD722 pKa = 4.09 NDD724 pKa = 3.45 LSRR727 pKa = 11.84 EE728 pKa = 4.09 NYY730 pKa = 10.36 DD731 pKa = 3.8 KK732 pKa = 11.04 ILIEE736 pKa = 4.19 WSLLPNLQNGIQLGAKK752 pKa = 9.51 DD753 pKa = 3.7 VQFCLGKK760 pKa = 10.13 DD761 pKa = 3.24 ARR763 pKa = 11.84 DD764 pKa = 3.36 ILTTIHH770 pKa = 6.07 NWNIIDD776 pKa = 3.51 QGEE779 pKa = 4.24 NCEE782 pKa = 4.1 EE783 pKa = 3.78 EE784 pKa = 4.72 RR785 pKa = 11.84 PFVTTWKK792 pKa = 8.9 TDD794 pKa = 3.22 NPGFSDD800 pKa = 3.6 NNQITIPTNPNKK812 pKa = 10.17 VYY814 pKa = 10.46 LYY816 pKa = 10.76 NVDD819 pKa = 3.38 WGDD822 pKa = 3.43 GTFDD826 pKa = 3.21 ISVTKK831 pKa = 10.44 DD832 pKa = 2.67 ITHH835 pKa = 7.07 TYY837 pKa = 9.49 VEE839 pKa = 4.6 PGTYY843 pKa = 9.08 QVSISGRR850 pKa = 11.84 FPHH853 pKa = 7.52 IYY855 pKa = 10.11 FNNSGEE861 pKa = 4.6 DD862 pKa = 3.89 YY863 pKa = 10.7 PHH865 pKa = 6.41 QAPKK869 pKa = 9.1 NTSDD873 pKa = 3.1 IEE875 pKa = 4.32 KK876 pKa = 10.08 IISIDD881 pKa = 3.11 QWGTNRR887 pKa = 11.84 WISMAFAFAGCSNLDD902 pKa = 3.49 VLATDD907 pKa = 4.21 IPDD910 pKa = 3.79 FSKK913 pKa = 11.16 DD914 pKa = 2.83 IDD916 pKa = 4.07 LSFMFYY922 pKa = 10.81 GCSSLKK928 pKa = 10.69 GNEE931 pKa = 4.27 SMEE934 pKa = 4.12 LWDD937 pKa = 5.25 LSDD940 pKa = 3.35 ITMSTPSMFSGAIQFNQSLGNWDD963 pKa = 3.48 VSNIRR968 pKa = 11.84 YY969 pKa = 9.04 LDD971 pKa = 3.34 RR972 pKa = 11.84 MFEE975 pKa = 4.03 NAISFNQNLEE985 pKa = 3.5 NWDD988 pKa = 3.33 IGKK991 pKa = 9.51 VEE993 pKa = 4.49 NMDD996 pKa = 3.76 AMFNGSGISIANYY1009 pKa = 10.09 DD1010 pKa = 3.84 RR1011 pKa = 11.84 LLNAWSTLPSLVNNVNLGAADD1032 pKa = 3.88 TNFCNSTEE1040 pKa = 3.82 ARR1042 pKa = 11.84 QNLIDD1047 pKa = 3.65 TYY1049 pKa = 10.94 GWQITDD1055 pKa = 3.93 AGNDD1059 pKa = 3.34 CSGTYY1064 pKa = 10.43 FITTWKK1070 pKa = 8.6 TDD1072 pKa = 3.32 NPGISADD1079 pKa = 3.77 NQITIPTFPSEE1090 pKa = 4.26 TYY1092 pKa = 10.02 DD1093 pKa = 4.06 YY1094 pKa = 11.18 SVNWGDD1100 pKa = 5.26 GNIEE1104 pKa = 4.59 SNFIGDD1110 pKa = 3.56 ATHH1113 pKa = 7.3 TYY1115 pKa = 7.63 LTPGTYY1121 pKa = 9.15 QVSISGVFPRR1131 pKa = 11.84 IFFNNFDD1138 pKa = 4.09 GTSSDD1143 pKa = 3.04 SDD1145 pKa = 3.84 KK1146 pKa = 10.98 IISVDD1151 pKa = 3.06 QWGDD1155 pKa = 3.58 IEE1157 pKa = 4.38 WTSMNNAFTNCTNLNVVAIDD1177 pKa = 3.66 IPNLEE1182 pKa = 4.43 NVNSLGAMFRR1192 pKa = 11.84 FCDD1195 pKa = 3.59 SLIGNEE1201 pKa = 5.09 SFSKK1205 pKa = 9.71 WQVKK1209 pKa = 9.97 SINFFDD1215 pKa = 4.21 FLFDD1219 pKa = 3.63 GASQFNQYY1227 pKa = 9.17 IGNWDD1232 pKa = 3.43 VSNATNMSYY1241 pKa = 9.69 MFNNATIFDD1250 pKa = 3.87 QDD1252 pKa = 3.04 ITNWDD1257 pKa = 3.64 ISNVDD1262 pKa = 3.69 HH1263 pKa = 7.19 IGLMFANTKK1272 pKa = 10.29 AFNQPIGNWNMSNLTSLQAIFAGASAFNQDD1302 pKa = 2.5 ISSWDD1307 pKa = 3.43 VSNVTNMEE1315 pKa = 3.93 GMFSGAISFNQDD1327 pKa = 1.81 ISNWDD1332 pKa = 3.45 VSNVTTMNSMFPGAISFNSPLNSWDD1357 pKa = 3.51 VSSVTDD1363 pKa = 3.51 MTAMFDD1369 pKa = 3.43 GATSFNRR1376 pKa = 11.84 PLNNWNISNVTQISSMFVNATSFNQPLDD1404 pKa = 3.32 NWDD1407 pKa = 3.57 VSNVTSLAGMFLKK1420 pKa = 10.75 ASSFNQNLSNWDD1432 pKa = 3.23 ISNVTSIGGMFNQAISYY1449 pKa = 8.31 DD1450 pKa = 3.92 QNLGDD1455 pKa = 3.87 WDD1457 pKa = 3.74 VSKK1460 pKa = 10.57 IINMDD1465 pKa = 3.56 YY1466 pKa = 10.75 LFNGIGLSLDD1476 pKa = 4.16 NYY1478 pKa = 11.16 DD1479 pKa = 3.65 KK1480 pKa = 11.2 TLTGWANLPSLQNNVVFDD1498 pKa = 4.07 AANSKK1503 pKa = 10.46 YY1504 pKa = 10.41 CEE1506 pKa = 4.26 SEE1508 pKa = 3.77 NARR1511 pKa = 11.84 QSIIDD1516 pKa = 3.64 TFGWTINDD1524 pKa = 3.39 FGKK1527 pKa = 10.81 VPFCNEE1533 pKa = 4.0 DD1534 pKa = 3.26 NDD1536 pKa = 4.4 LDD1538 pKa = 5.31 GILDD1542 pKa = 4.17 HH1543 pKa = 7.43 KK1544 pKa = 10.93 DD1545 pKa = 3.03 SCLDD1549 pKa = 3.16 TRR1551 pKa = 11.84 PNVTVNDD1558 pKa = 3.97 NGCEE1562 pKa = 3.95 IIVANAILVYY1572 pKa = 9.99 GASPTCPGEE1581 pKa = 4.03 ANGTIEE1587 pKa = 4.95 ISSNLINYY1595 pKa = 7.49 GFNISIEE1602 pKa = 4.38 GPVSFDD1608 pKa = 3.39 YY1609 pKa = 11.72 NEE1611 pKa = 3.82 ISLNNNLEE1619 pKa = 4.01 VSNLTTGMYY1628 pKa = 8.83 TVTIAIPDD1636 pKa = 3.35 ISYY1639 pKa = 9.51 VQTYY1643 pKa = 9.03 GIQINEE1649 pKa = 3.93 VDD1651 pKa = 3.98 TISGKK1656 pKa = 10.19 RR1657 pKa = 11.84 EE1658 pKa = 3.87 NLNTSAKK1665 pKa = 7.61 TALYY1669 pKa = 9.99 NVEE1672 pKa = 3.62 GSYY1675 pKa = 11.17 NYY1677 pKa = 9.7 TVDD1680 pKa = 4.19 VNGEE1684 pKa = 3.95 LKK1686 pKa = 10.78 NYY1688 pKa = 9.8 NFTSNGVNEE1697 pKa = 4.41 IQLSDD1702 pKa = 3.59 LGEE1705 pKa = 4.3 INTISISGEE1714 pKa = 3.72 SDD1716 pKa = 3.44 CQGKK1720 pKa = 7.96 VTDD1723 pKa = 4.79 SFAFTDD1729 pKa = 4.14 GLIMYY1734 pKa = 7.35 PTITTGEE1741 pKa = 4.3 VFVEE1745 pKa = 4.89 GCDD1748 pKa = 3.48 EE1749 pKa = 4.3 SSTVLVYY1756 pKa = 10.69 DD1757 pKa = 3.61 LSGRR1761 pKa = 11.84 LVFSKK1766 pKa = 9.57 TLSPQDD1772 pKa = 3.86 SNSIDD1777 pKa = 3.23 IQALEE1782 pKa = 4.14 TGVYY1786 pKa = 10.27 PMVIQSKK1793 pKa = 9.87 EE1794 pKa = 3.57 NSKK1797 pKa = 8.08 TFKK1800 pKa = 10.33 IIKK1803 pKa = 8.78 QQ1804 pKa = 3.43
Molecular weight: 201.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.884
IPC_protein 3.935
Toseland 3.694
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.859
Rodwell 3.745
Grimsley 3.605
Solomon 3.923
Lehninger 3.872
Nozaki 4.024
DTASelect 4.304
Thurlkill 3.745
EMBOSS 3.872
Sillero 4.05
Patrickios 1.329
IPC_peptide 3.91
IPC2_peptide 4.024
IPC2.peptide.svr19 3.921
Protein with the highest isoelectric point:
>tr|A0A1B7Z1R9|A0A1B7Z1R9_9FLAO LPS export ABC transporter ATP-binding protein OS=Maribacter sp. T28 OX=1836467 GN=A9200_09685 PE=4 SV=1
MM1 pKa = 7.02 YY2 pKa = 10.31 LVRR5 pKa = 11.84 KK6 pKa = 10.0 INFTDD11 pKa = 2.98 NGQRR15 pKa = 11.84 TTDD18 pKa = 3.03 NGQRR22 pKa = 11.84 TTDD25 pKa = 3.03 NGQRR29 pKa = 11.84 TTDD32 pKa = 3.03 NGQRR36 pKa = 11.84 TTDD39 pKa = 3.03 NGQRR43 pKa = 11.84 TTDD46 pKa = 3.03 NGQRR50 pKa = 11.84 TTDD53 pKa = 3.03 NGQRR57 pKa = 11.84 TTDD60 pKa = 3.03 NGQRR64 pKa = 11.84 TTDD67 pKa = 3.03 NGQRR71 pKa = 11.84 TTDD74 pKa = 3.03 NGQRR78 pKa = 11.84 TTDD81 pKa = 3.03 NGQRR85 pKa = 11.84 TTDD88 pKa = 3.03 NGQRR92 pKa = 11.84 TTDD95 pKa = 3.03 NGQRR99 pKa = 11.84 TTDD102 pKa = 3.03 NGQRR106 pKa = 11.84 TTDD109 pKa = 3.03 NGQRR113 pKa = 11.84 TTDD116 pKa = 3.03 NGQRR120 pKa = 11.84 TTDD123 pKa = 3.03 NGQRR127 pKa = 11.84 TTDD130 pKa = 3.03 NGQRR134 pKa = 11.84 TTDD137 pKa = 3.03 NGQRR141 pKa = 11.84 TTDD144 pKa = 3.03 NGQRR148 pKa = 11.84 TTDD151 pKa = 3.03 NGQRR155 pKa = 11.84 TTDD158 pKa = 3.03 NGQRR162 pKa = 11.84 TTDD165 pKa = 3.03 NGQRR169 pKa = 11.84 TTDD172 pKa = 3.03 NGQRR176 pKa = 11.84 TTDD179 pKa = 3.03 NGQRR183 pKa = 11.84 TTDD186 pKa = 3.03 NGQRR190 pKa = 11.84 TTDD193 pKa = 3.03 NGQRR197 pKa = 11.84 TTDD200 pKa = 3.03 NGQRR204 pKa = 11.84 TTDD207 pKa = 3.03 NGQRR211 pKa = 11.84 TTDD214 pKa = 3.03 NGQRR218 pKa = 11.84 TTDD221 pKa = 3.03 NGQRR225 pKa = 11.84 KK226 pKa = 8.83 ARR228 pKa = 11.84 FNLKK232 pKa = 10.45 LILNLRR238 pKa = 11.84 LYY240 pKa = 10.82
Molecular weight: 26.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.268
IPC_protein 10.511
Toseland 10.628
ProMoST 10.73
Dawson 10.687
Bjellqvist 10.54
Wikipedia 11.023
Rodwell 10.526
Grimsley 10.745
Solomon 10.979
Lehninger 10.921
Nozaki 10.613
DTASelect 10.54
Thurlkill 10.628
EMBOSS 11.082
Sillero 10.643
Patrickios 10.277
IPC_peptide 10.994
IPC2_peptide 9.706
IPC2.peptide.svr19 8.98
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3578
0
3578
1244144
42
3735
347.7
39.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.336 ± 0.035
0.71 ± 0.013
5.661 ± 0.033
6.676 ± 0.03
5.157 ± 0.031
6.572 ± 0.041
1.723 ± 0.018
7.978 ± 0.045
7.654 ± 0.053
9.418 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.286 ± 0.023
6.226 ± 0.045
3.418 ± 0.022
3.215 ± 0.02
3.319 ± 0.023
6.536 ± 0.03
5.837 ± 0.045
6.197 ± 0.033
1.098 ± 0.015
3.985 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here