Amino acid dipepetide frequency for Pseudoxanthomonas sp. GM95

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.518AlaAla: 17.518 ± 0.176	1.226AlaCys: 1.226 ± 0.035	7.031AlaAsp: 7.031 ± 0.083	6.173AlaGlu: 6.173 ± 0.079	4.064AlaPhe: 4.064 ± 0.065	11.055AlaGly: 11.055 ± 0.095	2.643AlaHis: 2.643 ± 0.052	5.341AlaIle: 5.341 ± 0.067	3.808AlaLys: 3.808 ± 0.072	15.675AlaLeu: 15.675 ± 0.157	3.449AlaMet: 3.449 ± 0.057	2.979AlaAsn: 2.979 ± 0.056	6.885AlaPro: 6.885 ± 0.107	6.256AlaGln: 6.256 ± 0.083	8.915AlaArg: 8.915 ± 0.116	7.261AlaSer: 7.261 ± 0.081	6.83AlaThr: 6.83 ± 0.09	8.75AlaVal: 8.75 ± 0.093	2.098AlaTrp: 2.098 ± 0.048	2.6AlaTyr: 2.6 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	1.074CysAla: 1.074 ± 0.032	0.103CysCys: 0.103 ± 0.009	0.462CysAsp: 0.462 ± 0.022	0.386CysGlu: 0.386 ± 0.017	0.252CysPhe: 0.252 ± 0.014	0.822CysGly: 0.822 ± 0.026	0.204CysHis: 0.204 ± 0.013	0.311CysIle: 0.311 ± 0.017	0.205CysLys: 0.205 ± 0.012	0.777CysLeu: 0.777 ± 0.025	0.158CysMet: 0.158 ± 0.011	0.221CysAsn: 0.221 ± 0.012	0.35CysPro: 0.35 ± 0.016	0.236CysGln: 0.236 ± 0.014	0.472CysArg: 0.472 ± 0.015	0.455CysSer: 0.455 ± 0.018	0.473CysThr: 0.473 ± 0.018	0.674CysVal: 0.674 ± 0.024	0.126CysTrp: 0.126 ± 0.009	0.176CysTyr: 0.176 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	8.718AspAla: 8.718 ± 0.095	0.385AspCys: 0.385 ± 0.019	3.368AspAsp: 3.368 ± 0.051	2.954AspGlu: 2.954 ± 0.049	2.116AspPhe: 2.116 ± 0.045	5.79AspGly: 5.79 ± 0.083	1.286AspHis: 1.286 ± 0.033	2.301AspIle: 2.301 ± 0.046	1.69AspLys: 1.69 ± 0.046	5.73AspLeu: 5.73 ± 0.063	1.129AspMet: 1.129 ± 0.033	1.485AspAsn: 1.485 ± 0.032	3.375AspPro: 3.375 ± 0.049	1.99AspGln: 1.99 ± 0.045	3.523AspArg: 3.523 ± 0.05	2.538AspSer: 2.538 ± 0.05	3.263AspThr: 3.263 ± 0.048	4.303AspVal: 4.303 ± 0.056	1.152AspTrp: 1.152 ± 0.029	1.744AspTyr: 1.744 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	6.524GluAla: 6.524 ± 0.092	0.29GluCys: 0.29 ± 0.017	2.566GluAsp: 2.566 ± 0.042	2.098GluGlu: 2.098 ± 0.046	1.513GluPhe: 1.513 ± 0.034	3.89GluGly: 3.89 ± 0.049	1.326GluHis: 1.326 ± 0.033	2.175GluIle: 2.175 ± 0.041	1.414GluLys: 1.414 ± 0.037	5.58GluLeu: 5.58 ± 0.07	0.92GluMet: 0.92 ± 0.028	1.126GluAsn: 1.126 ± 0.031	2.192GluPro: 2.192 ± 0.05	2.433GluGln: 2.433 ± 0.044	4.163GluArg: 4.163 ± 0.06	2.313GluSer: 2.313 ± 0.039	2.567GluThr: 2.567 ± 0.04	3.67GluVal: 3.67 ± 0.05	0.594GluTrp: 0.594 ± 0.022	1.047GluTyr: 1.047 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	4.164PheAla: 4.164 ± 0.063	0.31PheCys: 0.31 ± 0.015	2.757PheAsp: 2.757 ± 0.046	1.818PheGlu: 1.818 ± 0.042	1.229PhePhe: 1.229 ± 0.035	3.44PheGly: 3.44 ± 0.054	0.724PheHis: 0.724 ± 0.026	1.305PheIle: 1.305 ± 0.034	1.012PheLys: 1.012 ± 0.032	2.924PheLeu: 2.924 ± 0.052	0.66PheMet: 0.66 ± 0.026	1.219PheAsn: 1.219 ± 0.033	1.46PhePro: 1.46 ± 0.034	1.014PheGln: 1.014 ± 0.027	1.862PheArg: 1.862 ± 0.041	2.12PheSer: 2.12 ± 0.041	1.888PheThr: 1.888 ± 0.045	2.535PheVal: 2.535 ± 0.044	0.505PheTrp: 0.505 ± 0.019	0.873PheTyr: 0.873 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	9.589GlyAla: 9.589 ± 0.109	0.771GlyCys: 0.771 ± 0.027	4.662GlyAsp: 4.662 ± 0.061	4.445GlyGlu: 4.445 ± 0.058	3.412GlyPhe: 3.412 ± 0.051	7.234GlyGly: 7.234 ± 0.099	2.06GlyHis: 2.06 ± 0.045	3.937GlyIle: 3.937 ± 0.062	3.234GlyLys: 3.234 ± 0.054	8.995GlyLeu: 8.995 ± 0.098	2.234GlyMet: 2.234 ± 0.044	2.474GlyAsn: 2.474 ± 0.064	3.068GlyPro: 3.068 ± 0.049	3.624GlyGln: 3.624 ± 0.05	5.498GlyArg: 5.498 ± 0.066	4.734GlySer: 4.734 ± 0.077	4.971GlyThr: 4.971 ± 0.083	6.676GlyVal: 6.676 ± 0.084	1.676GlyTrp: 1.676 ± 0.035	2.493GlyTyr: 2.493 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	3.041HisAla: 3.041 ± 0.054	0.219HisCys: 0.219 ± 0.014	1.458HisAsp: 1.458 ± 0.034	1.021HisGlu: 1.021 ± 0.027	0.836HisPhe: 0.836 ± 0.028	2.3HisGly: 2.3 ± 0.041	0.578HisHis: 0.578 ± 0.023	0.765HisIle: 0.765 ± 0.024	0.46HisLys: 0.46 ± 0.02	2.15HisLeu: 2.15 ± 0.045	0.463HisMet: 0.463 ± 0.019	0.53HisAsn: 0.53 ± 0.019	1.353HisPro: 1.353 ± 0.036	0.693HisGln: 0.693 ± 0.024	1.461HisArg: 1.461 ± 0.034	0.943HisSer: 0.943 ± 0.026	1.036HisThr: 1.036 ± 0.028	1.646HisVal: 1.646 ± 0.041	0.474HisTrp: 0.474 ± 0.02	0.639HisTyr: 0.639 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	6.487IleAla: 6.487 ± 0.071	0.362IleCys: 0.362 ± 0.02	3.185IleAsp: 3.185 ± 0.049	2.684IleGlu: 2.684 ± 0.049	1.14IlePhe: 1.14 ± 0.033	4.355IleGly: 4.355 ± 0.059	0.76IleHis: 0.76 ± 0.023	1.3IleIle: 1.3 ± 0.039	1.238IleLys: 1.238 ± 0.03	2.977IleLeu: 2.977 ± 0.054	0.513IleMet: 0.513 ± 0.022	1.302IleAsn: 1.302 ± 0.034	2.001IlePro: 2.001 ± 0.038	1.18IleGln: 1.18 ± 0.031	2.234IleArg: 2.234 ± 0.042	2.277IleSer: 2.277 ± 0.041	2.475IleThr: 2.475 ± 0.052	3.335IleVal: 3.335 ± 0.052	0.448IleTrp: 0.448 ± 0.021	0.865IleTyr: 0.865 ± 0.025	0.0IleXaa: 0.0 ± 0.0
Lys	3.796LysAla: 3.796 ± 0.071	0.127LysCys: 0.127 ± 0.011	1.584LysAsp: 1.584 ± 0.038	1.11LysGlu: 1.11 ± 0.031	0.869LysPhe: 0.869 ± 0.026	2.115LysGly: 2.115 ± 0.038	0.616LysHis: 0.616 ± 0.02	1.245LysIle: 1.245 ± 0.034	1.049LysLys: 1.049 ± 0.051	3.272LysLeu: 3.272 ± 0.055	0.599LysMet: 0.599 ± 0.019	0.662LysAsn: 0.662 ± 0.024	2.119LysPro: 2.119 ± 0.045	1.363LysGln: 1.363 ± 0.033	2.107LysArg: 2.107 ± 0.047	1.477LysSer: 1.477 ± 0.039	1.771LysThr: 1.771 ± 0.039	2.345LysVal: 2.345 ± 0.049	0.318LysTrp: 0.318 ± 0.015	0.631LysTyr: 0.631 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	14.529LeuAla: 14.529 ± 0.156	0.995LeuCys: 0.995 ± 0.029	6.795LeuAsp: 6.795 ± 0.079	5.161LeuGlu: 5.161 ± 0.072	3.344LeuPhe: 3.344 ± 0.055	8.767LeuGly: 8.767 ± 0.091	2.455LeuHis: 2.455 ± 0.049	4.092LeuIle: 4.092 ± 0.058	3.29LeuLys: 3.29 ± 0.054	11.697LeuLeu: 11.697 ± 0.15	2.286LeuMet: 2.286 ± 0.045	2.38LeuAsn: 2.38 ± 0.042	6.538LeuPro: 6.538 ± 0.079	4.304LeuGln: 4.304 ± 0.062	7.99LeuArg: 7.99 ± 0.099	6.446LeuSer: 6.446 ± 0.079	5.29LeuThr: 5.29 ± 0.072	7.918LeuVal: 7.918 ± 0.095	1.445LeuTrp: 1.445 ± 0.039	2.235LeuTyr: 2.235 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	2.756MetAla: 2.756 ± 0.05	0.131MetCys: 0.131 ± 0.009	1.166MetAsp: 1.166 ± 0.029	0.925MetGlu: 0.925 ± 0.025	0.63MetPhe: 0.63 ± 0.02	1.606MetGly: 1.606 ± 0.039	0.458MetHis: 0.458 ± 0.017	0.883MetIle: 0.883 ± 0.024	0.775MetLys: 0.775 ± 0.023	2.356MetLeu: 2.356 ± 0.041	0.441MetMet: 0.441 ± 0.018	0.604MetAsn: 0.604 ± 0.026	1.436MetPro: 1.436 ± 0.034	0.967MetGln: 0.967 ± 0.025	1.683MetArg: 1.683 ± 0.035	1.654MetSer: 1.654 ± 0.031	1.47MetThr: 1.47 ± 0.03	1.439MetVal: 1.439 ± 0.032	0.201MetTrp: 0.201 ± 0.011	0.35MetTyr: 0.35 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	3.526AsnAla: 3.526 ± 0.062	0.184AsnCys: 0.184 ± 0.017	1.519AsnAsp: 1.519 ± 0.047	1.044AsnGlu: 1.044 ± 0.029	0.948AsnPhe: 0.948 ± 0.029	2.481AsnGly: 2.481 ± 0.053	0.485AsnHis: 0.485 ± 0.022	1.057AsnIle: 1.057 ± 0.03	0.686AsnLys: 0.686 ± 0.023	2.513AsnLeu: 2.513 ± 0.051	0.447AsnMet: 0.447 ± 0.018	0.709AsnAsn: 0.709 ± 0.028	1.737AsnPro: 1.737 ± 0.036	0.873AsnGln: 0.873 ± 0.025	1.566AsnArg: 1.566 ± 0.037	1.209AsnSer: 1.209 ± 0.037	1.507AsnThr: 1.507 ± 0.04	2.013AsnVal: 2.013 ± 0.056	0.404AsnTrp: 0.404 ± 0.019	0.752AsnTyr: 0.752 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	7.417ProAla: 7.417 ± 0.086	0.278ProCys: 0.278 ± 0.015	3.506ProAsp: 3.506 ± 0.058	3.067ProGlu: 3.067 ± 0.05	1.61ProPhe: 1.61 ± 0.032	4.653ProGly: 4.653 ± 0.051	1.094ProHis: 1.094 ± 0.031	1.969ProIle: 1.969 ± 0.039	1.566ProLys: 1.566 ± 0.037	5.525ProLeu: 5.525 ± 0.071	1.348ProMet: 1.348 ± 0.029	1.303ProAsn: 1.303 ± 0.032	2.72ProPro: 2.72 ± 0.068	2.334ProGln: 2.334 ± 0.045	3.208ProArg: 3.208 ± 0.057	2.862ProSer: 2.862 ± 0.053	2.688ProThr: 2.688 ± 0.042	4.185ProVal: 4.185 ± 0.065	0.83ProTrp: 0.83 ± 0.027	1.221ProTyr: 1.221 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	6.13GlnAla: 6.13 ± 0.097	0.273GlnCys: 0.273 ± 0.012	1.948GlnAsp: 1.948 ± 0.04	1.402GlnGlu: 1.402 ± 0.035	1.256GlnPhe: 1.256 ± 0.034	2.98GlnGly: 2.98 ± 0.048	0.985GlnHis: 0.985 ± 0.025	1.717GlnIle: 1.717 ± 0.037	0.915GlnLys: 0.915 ± 0.026	4.693GlnLeu: 4.693 ± 0.07	0.87GlnMet: 0.87 ± 0.024	0.724GlnAsn: 0.724 ± 0.027	2.368GlnPro: 2.368 ± 0.045	2.105GlnGln: 2.105 ± 0.051	3.591GlnArg: 3.591 ± 0.064	1.916GlnSer: 1.916 ± 0.039	1.928GlnThr: 1.928 ± 0.036	3.584GlnVal: 3.584 ± 0.046	0.724GlnTrp: 0.724 ± 0.024	0.921GlnTyr: 0.921 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	7.895ArgAla: 7.895 ± 0.095	0.471ArgCys: 0.471 ± 0.019	4.231ArgAsp: 4.231 ± 0.058	3.756ArgGlu: 3.756 ± 0.059	2.717ArgPhe: 2.717 ± 0.044	4.922ArgGly: 4.922 ± 0.055	1.7ArgHis: 1.7 ± 0.038	3.374ArgIle: 3.374 ± 0.054	1.978ArgLys: 1.978 ± 0.039	7.572ArgLeu: 7.572 ± 0.097	1.757ArgMet: 1.757 ± 0.037	1.881ArgAsn: 1.881 ± 0.04	3.04ArgPro: 3.04 ± 0.049	2.899ArgGln: 2.899 ± 0.047	5.038ArgArg: 5.038 ± 0.077	3.459ArgSer: 3.459 ± 0.055	3.363ArgThr: 3.363 ± 0.053	5.024ArgVal: 5.024 ± 0.072	1.294ArgTrp: 1.294 ± 0.031	2.112ArgTyr: 2.112 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	6.744SerAla: 6.744 ± 0.082	0.395SerCys: 0.395 ± 0.019	3.002SerAsp: 3.002 ± 0.046	2.458SerGlu: 2.458 ± 0.044	1.966SerPhe: 1.966 ± 0.044	5.279SerGly: 5.279 ± 0.069	1.13SerHis: 1.13 ± 0.027	2.246SerIle: 2.246 ± 0.039	1.545SerLys: 1.545 ± 0.039	5.691SerLeu: 5.691 ± 0.061	1.132SerMet: 1.132 ± 0.029	1.584SerAsn: 1.584 ± 0.04	3.001SerPro: 3.001 ± 0.053	2.06SerGln: 2.06 ± 0.044	3.431SerArg: 3.431 ± 0.054	3.168SerSer: 3.168 ± 0.052	3.227SerThr: 3.227 ± 0.055	4.004SerVal: 4.004 ± 0.07	0.859SerTrp: 0.859 ± 0.026	1.52SerTyr: 1.52 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	6.667ThrAla: 6.667 ± 0.103	0.38ThrCys: 0.38 ± 0.017	2.861ThrAsp: 2.861 ± 0.053	2.225ThrGlu: 2.225 ± 0.046	1.793ThrPhe: 1.793 ± 0.037	5.023ThrGly: 5.023 ± 0.075	1.181ThrHis: 1.181 ± 0.031	2.108ThrIle: 2.108 ± 0.047	1.235ThrLys: 1.235 ± 0.034	6.765ThrLeu: 6.765 ± 0.076	0.925ThrMet: 0.925 ± 0.032	1.233ThrAsn: 1.233 ± 0.035	3.951ThrPro: 3.951 ± 0.067	2.083ThrGln: 2.083 ± 0.044	3.536ThrArg: 3.536 ± 0.051	2.903ThrSer: 2.903 ± 0.053	3.073ThrThr: 3.073 ± 0.062	4.289ThrVal: 4.289 ± 0.079	0.783ThrTrp: 0.783 ± 0.026	1.333ThrTyr: 1.333 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	9.525ValAla: 9.525 ± 0.098	0.688ValCys: 0.688 ± 0.023	4.464ValAsp: 4.464 ± 0.065	4.053ValGlu: 4.053 ± 0.054	2.61ValPhe: 2.61 ± 0.047	5.759ValGly: 5.759 ± 0.084	1.545ValHis: 1.545 ± 0.034	3.438ValIle: 3.438 ± 0.048	1.915ValLys: 1.915 ± 0.044	8.516ValLeu: 8.516 ± 0.094	1.705ValMet: 1.705 ± 0.037	1.982ValAsn: 1.982 ± 0.043	3.888ValPro: 3.888 ± 0.059	2.864ValGln: 2.864 ± 0.045	4.938ValArg: 4.938 ± 0.065	4.425ValSer: 4.425 ± 0.064	4.236ValThr: 4.236 ± 0.078	6.153ValVal: 6.153 ± 0.08	0.945ValTrp: 0.945 ± 0.027	1.59ValTyr: 1.59 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	1.399TrpAla: 1.399 ± 0.037	0.147TrpCys: 0.147 ± 0.01	0.776TrpAsp: 0.776 ± 0.024	0.548TrpGlu: 0.548 ± 0.018	0.584TrpPhe: 0.584 ± 0.021	1.03TrpGly: 1.03 ± 0.031	0.39TrpHis: 0.39 ± 0.017	0.743TrpIle: 0.743 ± 0.022	0.576TrpLys: 0.576 ± 0.021	2.121TrpLeu: 2.121 ± 0.047	0.482TrpMet: 0.482 ± 0.02	0.517TrpAsn: 0.517 ± 0.023	0.76TrpPro: 0.76 ± 0.025	0.803TrpGln: 0.803 ± 0.025	1.303TrpArg: 1.303 ± 0.031	0.916TrpSer: 0.916 ± 0.026	0.836TrpThr: 0.836 ± 0.026	0.972TrpVal: 0.972 ± 0.027	0.334TrpTrp: 0.334 ± 0.017	0.376TrpTyr: 0.376 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.056TyrAla: 3.056 ± 0.048	0.203TyrCys: 0.203 ± 0.012	1.497TyrAsp: 1.497 ± 0.04	1.072TyrGlu: 1.072 ± 0.027	0.931TyrPhe: 0.931 ± 0.026	2.285TyrGly: 2.285 ± 0.05	0.447TyrHis: 0.447 ± 0.02	0.813TyrIle: 0.813 ± 0.028	0.583TyrLys: 0.583 ± 0.025	2.431TyrLeu: 2.431 ± 0.053	0.417TyrMet: 0.417 ± 0.017	0.746TyrAsn: 0.746 ± 0.03	1.138TyrPro: 1.138 ± 0.03	0.947TyrGln: 0.947 ± 0.026	1.965TyrArg: 1.965 ± 0.042	1.366TyrSer: 1.366 ± 0.036	1.455TyrThr: 1.455 ± 0.043	1.724TyrVal: 1.724 ± 0.036	0.401TyrTrp: 0.401 ± 0.019	0.738TyrTyr: 0.738 ± 0.032	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4071 proteins (1401671 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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