Eschrichtius robustus (California gray whale) (Eschrichtius gibbosus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria;

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10855 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2F0AY62|A0A2F0AY62_ESCRO GDP-fucose transporter 1 (Fragment) OS=Eschrichtius robustus OX=9764 GN=ESR_30128 PE=3 SV=1
MM1 pKa = 7.9DD2 pKa = 4.03HH3 pKa = 6.71TAFPCGCCRR12 pKa = 11.84EE13 pKa = 4.32GCEE16 pKa = 4.09NPKK19 pKa = 10.53GRR21 pKa = 11.84VEE23 pKa = 4.13FNQARR28 pKa = 11.84VQTHH32 pKa = 7.12FIHH35 pKa = 6.13TLTRR39 pKa = 11.84LQLEE43 pKa = 4.23QGAEE47 pKa = 4.07SLGEE51 pKa = 4.09LEE53 pKa = 5.72APAQGATFSPNEE65 pKa = 3.96QVLAPTFPLAKK76 pKa = 9.66PPVSSEE82 pKa = 4.27LGDD85 pKa = 3.79NSCSSDD91 pKa = 3.31MTDD94 pKa = 2.81SSTASSSSEE103 pKa = 3.9APSGSAHH110 pKa = 6.96PALPGPSFQPGVDD123 pKa = 3.76DD124 pKa = 5.97DD125 pKa = 4.2SLARR129 pKa = 11.84ILSFSDD135 pKa = 3.16SDD137 pKa = 3.74LGGDD141 pKa = 3.78GEE143 pKa = 4.36EE144 pKa = 4.38EE145 pKa = 4.26EE146 pKa = 4.39EE147 pKa = 4.64GGVGSLDD154 pKa = 3.42NLSCFHH160 pKa = 7.14PADD163 pKa = 3.3IFGTGDD169 pKa = 3.56PAGLASWTHH178 pKa = 6.1SYY180 pKa = 10.94SSSSLASGILDD191 pKa = 3.36EE192 pKa = 5.03NANLDD197 pKa = 3.57ASCFLNSGLEE207 pKa = 4.07SLGEE211 pKa = 4.1GSLPGPPVPAITDD224 pKa = 3.9ASQSSSVDD232 pKa = 3.62LSLSSCDD239 pKa = 3.95SFKK242 pKa = 11.06LLQALPDD249 pKa = 3.89YY250 pKa = 11.07SLGPHH255 pKa = 5.27YY256 pKa = 9.71TSQKK260 pKa = 10.32VSDD263 pKa = 3.91SLDD266 pKa = 3.48NLEE269 pKa = 4.97APHH272 pKa = 6.36FALPAFSPPGDD283 pKa = 3.41ASTCFLEE290 pKa = 5.53SIMGLPEE297 pKa = 3.84PAAEE301 pKa = 4.02ALAPFMDD308 pKa = 4.17GQLFEE313 pKa = 4.63DD314 pKa = 4.29TAPASLVEE322 pKa = 4.21PVSVV326 pKa = 3.42

Molecular weight: 33.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2F0BK24|A0A2F0BK24_ESCRO 40S ribosomal protein S3 (Fragment) OS=Eschrichtius robustus OX=9764 GN=ESR_29911 PE=3 SV=1
MM1 pKa = 6.82MRR3 pKa = 11.84SSALLSSRR11 pKa = 11.84MPVQASSRR19 pKa = 11.84PWPRR23 pKa = 11.84ARR25 pKa = 11.84EE26 pKa = 3.92TEE28 pKa = 3.87RR29 pKa = 11.84RR30 pKa = 11.84EE31 pKa = 4.11GCLQVCKK38 pKa = 10.74ARR40 pKa = 11.84GKK42 pKa = 10.1DD43 pKa = 3.34QSQRR47 pKa = 11.84PLRR50 pKa = 11.84RR51 pKa = 11.84PRR53 pKa = 11.84SPHH56 pKa = 5.61RR57 pKa = 11.84PRR59 pKa = 11.84WVYY62 pKa = 10.86QLTSVWMVFVVVASILTNGLVLAATMKK89 pKa = 10.23FKK91 pKa = 11.0KK92 pKa = 9.7LRR94 pKa = 11.84HH95 pKa = 5.28PLNWILVNLAIADD108 pKa = 3.97LAEE111 pKa = 4.34TIIAGTISIVNQMYY125 pKa = 9.65GYY127 pKa = 10.28FVLGHH132 pKa = 6.48PLCIVEE138 pKa = 5.28GYY140 pKa = 7.23TVCLWGVIEE149 pKa = 4.02AHH151 pKa = 6.37LL152 pKa = 4.08

Molecular weight: 17.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10855

0

10855

3464131

50

4033

319.1

35.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.451 ± 0.026

2.26 ± 0.016

4.705 ± 0.017

6.972 ± 0.032

3.642 ± 0.018

6.82 ± 0.027

2.632 ± 0.013

3.93 ± 0.022

5.24 ± 0.033

10.364 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.1 ± 0.011

3.225 ± 0.016

6.551 ± 0.038

4.72 ± 0.022

5.911 ± 0.023

8.245 ± 0.034

5.14 ± 0.018

6.249 ± 0.018

1.252 ± 0.009

2.593 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski