Gordonia phage LordFarquaad

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Attisvirus; unclassified Attisvirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y6EJW1|A0A4Y6EJW1_9CAUD Minor tail protein OS=Gordonia phage LordFarquaad OX=2588134 GN=17 PE=4 SV=1
MM1 pKa = 7.72SYY3 pKa = 9.13TVTAALVVAADD14 pKa = 3.95TEE16 pKa = 4.72GKK18 pKa = 10.42LNYY21 pKa = 9.23HH22 pKa = 6.06YY23 pKa = 10.34EE24 pKa = 4.39GAHH27 pKa = 5.63IAYY30 pKa = 10.27LSDD33 pKa = 3.2EE34 pKa = 4.21DD35 pKa = 4.8AEE37 pKa = 4.67RR38 pKa = 11.84FLADD42 pKa = 3.26GMVVEE47 pKa = 4.81TADD50 pKa = 3.93LADD53 pKa = 3.62EE54 pKa = 4.61AFIPLADD61 pKa = 4.57DD62 pKa = 4.25PVDD65 pKa = 4.14GGDD68 pKa = 3.66PQAPAGDD75 pKa = 3.97GARR78 pKa = 11.84PPKK81 pKa = 9.6TASKK85 pKa = 10.3DD86 pKa = 3.08AWVDD90 pKa = 3.79FAEE93 pKa = 4.47AKK95 pKa = 10.36GMSRR99 pKa = 11.84AEE101 pKa = 4.1AEE103 pKa = 4.46DD104 pKa = 3.62MSKK107 pKa = 11.33ADD109 pKa = 5.46LIQALGG115 pKa = 3.34

Molecular weight: 12.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y6EI25|A0A4Y6EI25_9CAUD Uncharacterized protein OS=Gordonia phage LordFarquaad OX=2588134 GN=70 PE=4 SV=1
MM1 pKa = 7.25TLATRR6 pKa = 11.84TTLADD11 pKa = 3.52ASQRR15 pKa = 11.84RR16 pKa = 11.84PGPDD20 pKa = 3.35PRR22 pKa = 11.84PTADD26 pKa = 2.77VGTRR30 pKa = 11.84TADD33 pKa = 3.38DD34 pKa = 3.36RR35 pKa = 11.84RR36 pKa = 11.84PRR38 pKa = 11.84PRR40 pKa = 11.84QFQPQCVTCQQRR52 pKa = 11.84PARR55 pKa = 11.84TRR57 pKa = 11.84GMCPACYY64 pKa = 9.42EE65 pKa = 4.27RR66 pKa = 11.84EE67 pKa = 3.99RR68 pKa = 11.84TRR70 pKa = 11.84QKK72 pKa = 11.27AYY74 pKa = 10.47GRR76 pKa = 11.84WEE78 pKa = 4.18SQHH81 pKa = 7.38VDD83 pKa = 3.15AQPVRR88 pKa = 11.84DD89 pKa = 4.65HH90 pKa = 6.14IHH92 pKa = 6.52ALRR95 pKa = 11.84EE96 pKa = 3.91AGISNKK102 pKa = 8.92RR103 pKa = 11.84LRR105 pKa = 11.84EE106 pKa = 3.98LTGVSVNTIQVLMTGRR122 pKa = 11.84PEE124 pKa = 4.34RR125 pKa = 11.84GHH127 pKa = 6.65GPTKK131 pKa = 10.23KK132 pKa = 10.16VLRR135 pKa = 11.84RR136 pKa = 11.84TADD139 pKa = 3.92RR140 pKa = 11.84ILAVQVPQLAFTVASPGRR158 pKa = 11.84IVPALGATRR167 pKa = 11.84RR168 pKa = 11.84LQALVANGYY177 pKa = 7.26SQRR180 pKa = 11.84EE181 pKa = 4.08LCRR184 pKa = 11.84RR185 pKa = 11.84LGWAWQGNATALFLGRR201 pKa = 11.84AEE203 pKa = 4.05YY204 pKa = 10.67VVARR208 pKa = 11.84RR209 pKa = 11.84ARR211 pKa = 11.84EE212 pKa = 3.82VADD215 pKa = 5.57LFTQLQMVPGTDD227 pKa = 2.75VRR229 pKa = 11.84ARR231 pKa = 11.84NRR233 pKa = 11.84ARR235 pKa = 11.84ANGWPAPLDD244 pKa = 3.55WDD246 pKa = 4.36EE247 pKa = 4.84DD248 pKa = 4.52TIDD251 pKa = 5.71DD252 pKa = 4.89PDD254 pKa = 3.75ATPAAAEE261 pKa = 3.82QDD263 pKa = 3.95RR264 pKa = 11.84PRR266 pKa = 11.84MATTLDD272 pKa = 3.36EE273 pKa = 5.15FEE275 pKa = 4.82WLLQCGEE282 pKa = 4.49ASDD285 pKa = 4.65AAAKK289 pKa = 10.32RR290 pKa = 11.84SGVSLSTIRR299 pKa = 11.84VYY301 pKa = 10.05YY302 pKa = 10.25ARR304 pKa = 11.84EE305 pKa = 3.87GRR307 pKa = 11.84DD308 pKa = 3.35VPRR311 pKa = 11.84QLAPPPPLPVAYY323 pKa = 9.19PRR325 pKa = 11.84ARR327 pKa = 11.84RR328 pKa = 11.84SQVASS333 pKa = 3.46

Molecular weight: 37.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

15556

37

1469

204.7

22.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.227 ± 0.642

0.926 ± 0.173

7.013 ± 0.251

6.049 ± 0.252

2.636 ± 0.188

8.492 ± 0.378

1.845 ± 0.212

4.808 ± 0.185

2.925 ± 0.199

7.592 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.025 ± 0.109

2.822 ± 0.224

5.644 ± 0.246

3.696 ± 0.214

7.598 ± 0.399

5.496 ± 0.19

6.962 ± 0.342

7.065 ± 0.27

1.961 ± 0.146

2.218 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski