Amino acid dipepetide frequency for Gordonia phage LordFarquaad

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.036AlaAla: 17.036 ± 2.41
1.093AlaCys: 1.093 ± 0.278
9.9AlaAsp: 9.9 ± 0.853
9.579AlaGlu: 9.579 ± 0.897
2.057AlaPhe: 2.057 ± 0.441
8.743AlaGly: 8.743 ± 0.826
2.314AlaHis: 2.314 ± 0.365
6.943AlaIle: 6.943 ± 0.722
3.472AlaLys: 3.472 ± 0.472
7.972AlaLeu: 7.972 ± 0.813
3.279AlaMet: 3.279 ± 0.472
4.436AlaAsn: 4.436 ± 0.536
5.336AlaPro: 5.336 ± 0.617
4.564AlaGln: 4.564 ± 0.63
8.422AlaArg: 8.422 ± 0.797
6.043AlaSer: 6.043 ± 0.642
8.615AlaThr: 8.615 ± 1.025
7.972AlaVal: 7.972 ± 0.805
1.864AlaTrp: 1.864 ± 0.338
2.636AlaTyr: 2.636 ± 0.357
0.0AlaXaa: 0.0 ± 0.0
Cys
0.9CysAla: 0.9 ± 0.301
0.129CysCys: 0.129 ± 0.077
0.9CysAsp: 0.9 ± 0.277
0.964CysGlu: 0.964 ± 0.264
0.129CysPhe: 0.129 ± 0.086
0.771CysGly: 0.771 ± 0.275
0.193CysHis: 0.193 ± 0.117
0.064CysIle: 0.064 ± 0.074
0.386CysLys: 0.386 ± 0.179
0.386CysLeu: 0.386 ± 0.128
0.129CysMet: 0.129 ± 0.077
0.129CysAsn: 0.129 ± 0.084
0.643CysPro: 0.643 ± 0.236
0.257CysGln: 0.257 ± 0.149
1.029CysArg: 1.029 ± 0.307
0.514CysSer: 0.514 ± 0.193
0.321CysThr: 0.321 ± 0.169
0.707CysVal: 0.707 ± 0.183
0.321CysTrp: 0.321 ± 0.143
0.386CysTyr: 0.386 ± 0.153
0.0CysXaa: 0.0 ± 0.0
Asp
7.779AspAla: 7.779 ± 0.719
0.514AspCys: 0.514 ± 0.185
5.529AspAsp: 5.529 ± 0.728
4.822AspGlu: 4.822 ± 0.602
1.993AspPhe: 1.993 ± 0.345
7.329AspGly: 7.329 ± 0.602
1.414AspHis: 1.414 ± 0.299
2.572AspIle: 2.572 ± 0.368
1.221AspLys: 1.221 ± 0.225
7.007AspLeu: 7.007 ± 0.738
1.286AspMet: 1.286 ± 0.335
2.122AspAsn: 2.122 ± 0.422
4.886AspPro: 4.886 ± 0.581
1.993AspGln: 1.993 ± 0.393
4.436AspArg: 4.436 ± 0.551
2.893AspSer: 2.893 ± 0.366
4.564AspThr: 4.564 ± 0.521
4.95AspVal: 4.95 ± 0.57
1.479AspTrp: 1.479 ± 0.319
1.35AspTyr: 1.35 ± 0.249
0.0AspXaa: 0.0 ± 0.0
Glu
7.843GluAla: 7.843 ± 0.687
0.836GluCys: 0.836 ± 0.237
2.507GluAsp: 2.507 ± 0.392
2.636GluGlu: 2.636 ± 0.491
2.636GluPhe: 2.636 ± 0.38
4.564GluGly: 4.564 ± 0.555
1.35GluHis: 1.35 ± 0.351
3.472GluIle: 3.472 ± 0.414
1.8GluLys: 1.8 ± 0.337
4.822GluLeu: 4.822 ± 0.585
1.479GluMet: 1.479 ± 0.309
1.35GluAsn: 1.35 ± 0.239
3.022GluPro: 3.022 ± 0.396
3.343GluGln: 3.343 ± 0.595
5.4GluArg: 5.4 ± 0.676
3.922GluSer: 3.922 ± 0.561
3.472GluThr: 3.472 ± 0.466
3.729GluVal: 3.729 ± 0.515
1.029GluTrp: 1.029 ± 0.264
1.286GluTyr: 1.286 ± 0.272
0.0GluXaa: 0.0 ± 0.0
Phe
3.214PheAla: 3.214 ± 0.438
0.064PheCys: 0.064 ± 0.069
2.957PheAsp: 2.957 ± 0.468
1.736PheGlu: 1.736 ± 0.342
0.707PhePhe: 0.707 ± 0.214
2.764PheGly: 2.764 ± 0.437
0.321PheHis: 0.321 ± 0.145
0.836PheIle: 0.836 ± 0.244
0.514PheLys: 0.514 ± 0.193
1.543PheLeu: 1.543 ± 0.259
0.386PheMet: 0.386 ± 0.143
0.579PheAsn: 0.579 ± 0.192
0.964PhePro: 0.964 ± 0.291
0.643PheGln: 0.643 ± 0.201
1.543PheArg: 1.543 ± 0.308
1.543PheSer: 1.543 ± 0.325
2.25PheThr: 2.25 ± 0.39
1.993PheVal: 1.993 ± 0.403
1.286PheTrp: 1.286 ± 0.314
0.514PheTyr: 0.514 ± 0.144
0.0PheXaa: 0.0 ± 0.0
Gly
8.036GlyAla: 8.036 ± 1.03
0.579GlyCys: 0.579 ± 0.288
6.107GlyAsp: 6.107 ± 0.567
5.657GlyGlu: 5.657 ± 0.602
1.864GlyPhe: 1.864 ± 0.313
7.779GlyGly: 7.779 ± 0.968
1.736GlyHis: 1.736 ± 0.323
4.243GlyIle: 4.243 ± 0.511
3.022GlyLys: 3.022 ± 0.551
5.786GlyLeu: 5.786 ± 0.641
1.736GlyMet: 1.736 ± 0.383
2.764GlyAsn: 2.764 ± 0.406
4.629GlyPro: 4.629 ± 0.41
3.022GlyGln: 3.022 ± 0.444
6.815GlyArg: 6.815 ± 0.757
4.822GlySer: 4.822 ± 0.717
5.786GlyThr: 5.786 ± 0.944
6.75GlyVal: 6.75 ± 0.668
1.093GlyTrp: 1.093 ± 0.263
2.7GlyTyr: 2.7 ± 0.362
0.0GlyXaa: 0.0 ± 0.0
His
1.8HisAla: 1.8 ± 0.323
0.257HisCys: 0.257 ± 0.107
1.221HisAsp: 1.221 ± 0.284
1.157HisGlu: 1.157 ± 0.309
0.321HisPhe: 0.321 ± 0.149
1.736HisGly: 1.736 ± 0.387
0.514HisHis: 0.514 ± 0.166
0.964HisIle: 0.964 ± 0.293
0.579HisLys: 0.579 ± 0.173
1.8HisLeu: 1.8 ± 0.404
0.064HisMet: 0.064 ± 0.063
0.514HisAsn: 0.514 ± 0.177
1.286HisPro: 1.286 ± 0.304
1.157HisGln: 1.157 ± 0.291
1.414HisArg: 1.414 ± 0.328
0.643HisSer: 0.643 ± 0.196
1.093HisThr: 1.093 ± 0.228
0.836HisVal: 0.836 ± 0.246
0.45HisTrp: 0.45 ± 0.163
0.643HisTyr: 0.643 ± 0.191
0.0HisXaa: 0.0 ± 0.0
Ile
5.786IleAla: 5.786 ± 0.617
0.386IleCys: 0.386 ± 0.158
3.922IleAsp: 3.922 ± 0.426
3.664IleGlu: 3.664 ± 0.379
1.157IlePhe: 1.157 ± 0.234
4.05IleGly: 4.05 ± 0.488
1.029IleHis: 1.029 ± 0.216
1.221IleIle: 1.221 ± 0.33
1.093IleLys: 1.093 ± 0.222
3.086IleLeu: 3.086 ± 0.436
0.579IleMet: 0.579 ± 0.184
1.35IleAsn: 1.35 ± 0.324
2.507IlePro: 2.507 ± 0.346
2.572IleGln: 2.572 ± 0.39
3.536IleArg: 3.536 ± 0.383
2.829IleSer: 2.829 ± 0.395
3.15IleThr: 3.15 ± 0.466
4.307IleVal: 4.307 ± 0.505
0.45IleTrp: 0.45 ± 0.228
1.414IleTyr: 1.414 ± 0.341
0.0IleXaa: 0.0 ± 0.0
Lys
3.664LysAla: 3.664 ± 0.709
0.129LysCys: 0.129 ± 0.09
1.736LysAsp: 1.736 ± 0.285
1.414LysGlu: 1.414 ± 0.28
0.964LysPhe: 0.964 ± 0.264
1.736LysGly: 1.736 ± 0.359
0.45LysHis: 0.45 ± 0.168
1.543LysIle: 1.543 ± 0.316
0.9LysLys: 0.9 ± 0.317
2.893LysLeu: 2.893 ± 0.572
0.514LysMet: 0.514 ± 0.209
0.964LysAsn: 0.964 ± 0.363
1.736LysPro: 1.736 ± 0.299
0.836LysGln: 0.836 ± 0.223
2.314LysArg: 2.314 ± 0.407
1.864LysSer: 1.864 ± 0.32
1.929LysThr: 1.929 ± 0.353
2.636LysVal: 2.636 ± 0.351
0.321LysTrp: 0.321 ± 0.161
0.707LysTyr: 0.707 ± 0.235
0.0LysXaa: 0.0 ± 0.0
Leu
10.479LeuAla: 10.479 ± 0.942
0.771LeuCys: 0.771 ± 0.237
6.236LeuAsp: 6.236 ± 0.717
3.922LeuGlu: 3.922 ± 0.534
1.993LeuPhe: 1.993 ± 0.579
7.393LeuGly: 7.393 ± 0.767
1.093LeuHis: 1.093 ± 0.281
3.407LeuIle: 3.407 ± 0.475
1.8LeuLys: 1.8 ± 0.331
4.95LeuLeu: 4.95 ± 0.681
1.607LeuMet: 1.607 ± 0.314
1.929LeuAsn: 1.929 ± 0.407
3.793LeuPro: 3.793 ± 0.429
2.186LeuGln: 2.186 ± 0.399
5.722LeuArg: 5.722 ± 0.823
4.886LeuSer: 4.886 ± 0.546
5.529LeuThr: 5.529 ± 0.717
4.95LeuVal: 4.95 ± 0.631
1.671LeuTrp: 1.671 ± 0.245
1.607LeuTyr: 1.607 ± 0.327
0.0LeuXaa: 0.0 ± 0.0
Met
2.893MetAla: 2.893 ± 0.529
0.45MetCys: 0.45 ± 0.168
0.836MetAsp: 0.836 ± 0.239
0.9MetGlu: 0.9 ± 0.215
0.45MetPhe: 0.45 ± 0.184
1.479MetGly: 1.479 ± 0.408
0.386MetHis: 0.386 ± 0.141
1.414MetIle: 1.414 ± 0.264
0.643MetLys: 0.643 ± 0.189
1.736MetLeu: 1.736 ± 0.299
0.321MetMet: 0.321 ± 0.145
0.707MetAsn: 0.707 ± 0.241
1.35MetPro: 1.35 ± 0.293
0.45MetGln: 0.45 ± 0.247
0.9MetArg: 0.9 ± 0.239
1.607MetSer: 1.607 ± 0.282
2.25MetThr: 2.25 ± 0.365
1.093MetVal: 1.093 ± 0.275
0.257MetTrp: 0.257 ± 0.146
0.129MetTyr: 0.129 ± 0.087
0.0MetXaa: 0.0 ± 0.0
Asn
3.086AsnAla: 3.086 ± 0.531
0.129AsnCys: 0.129 ± 0.092
1.607AsnAsp: 1.607 ± 0.216
1.221AsnGlu: 1.221 ± 0.333
0.9AsnPhe: 0.9 ± 0.374
3.214AsnGly: 3.214 ± 0.417
0.771AsnHis: 0.771 ± 0.235
1.414AsnIle: 1.414 ± 0.3
0.707AsnLys: 0.707 ± 0.203
2.507AsnLeu: 2.507 ± 0.458
0.45AsnMet: 0.45 ± 0.167
0.579AsnAsn: 0.579 ± 0.158
1.993AsnPro: 1.993 ± 0.461
1.35AsnGln: 1.35 ± 0.29
2.314AsnArg: 2.314 ± 0.461
1.286AsnSer: 1.286 ± 0.253
2.057AsnThr: 2.057 ± 0.437
1.286AsnVal: 1.286 ± 0.268
0.643AsnTrp: 0.643 ± 0.163
0.707AsnTyr: 0.707 ± 0.182
0.0AsnXaa: 0.0 ± 0.0
Pro
7.007ProAla: 7.007 ± 0.896
0.643ProCys: 0.643 ± 0.228
4.629ProAsp: 4.629 ± 0.623
4.436ProGlu: 4.436 ± 0.675
0.836ProPhe: 0.836 ± 0.216
5.336ProGly: 5.336 ± 0.625
1.093ProHis: 1.093 ± 0.25
2.122ProIle: 2.122 ± 0.358
1.929ProLys: 1.929 ± 0.418
3.15ProLeu: 3.15 ± 0.446
1.35ProMet: 1.35 ± 0.241
2.186ProAsn: 2.186 ± 0.49
2.957ProPro: 2.957 ± 0.404
1.479ProGln: 1.479 ± 0.346
4.307ProArg: 4.307 ± 0.7
2.957ProSer: 2.957 ± 0.38
3.793ProThr: 3.793 ± 0.7
3.664ProVal: 3.664 ± 0.546
1.35ProTrp: 1.35 ± 0.312
1.221ProTyr: 1.221 ± 0.234
0.0ProXaa: 0.0 ± 0.0
Gln
3.857GlnAla: 3.857 ± 0.555
0.45GlnCys: 0.45 ± 0.18
1.607GlnAsp: 1.607 ± 0.257
1.414GlnGlu: 1.414 ± 0.365
0.964GlnPhe: 0.964 ± 0.251
2.379GlnGly: 2.379 ± 0.421
0.964GlnHis: 0.964 ± 0.215
2.379GlnIle: 2.379 ± 0.305
1.607GlnLys: 1.607 ± 0.412
3.407GlnLeu: 3.407 ± 0.462
1.607GlnMet: 1.607 ± 0.345
0.707GlnAsn: 0.707 ± 0.189
1.993GlnPro: 1.993 ± 0.304
1.607GlnGln: 1.607 ± 0.414
3.793GlnArg: 3.793 ± 0.656
1.479GlnSer: 1.479 ± 0.26
2.314GlnThr: 2.314 ± 0.379
2.893GlnVal: 2.893 ± 0.474
0.643GlnTrp: 0.643 ± 0.214
0.836GlnTyr: 0.836 ± 0.247
0.0GlnXaa: 0.0 ± 0.0
Arg
8.679ArgAla: 8.679 ± 0.739
0.643ArgCys: 0.643 ± 0.321
3.664ArgAsp: 3.664 ± 0.466
4.822ArgGlu: 4.822 ± 0.78
2.443ArgPhe: 2.443 ± 0.382
4.822ArgGly: 4.822 ± 0.542
0.964ArgHis: 0.964 ± 0.297
4.05ArgIle: 4.05 ± 0.475
2.829ArgLys: 2.829 ± 0.417
6.493ArgLeu: 6.493 ± 0.684
1.929ArgMet: 1.929 ± 0.318
2.379ArgAsn: 2.379 ± 0.333
4.693ArgPro: 4.693 ± 0.725
2.764ArgGln: 2.764 ± 0.393
6.75ArgArg: 6.75 ± 0.884
4.114ArgSer: 4.114 ± 0.498
4.757ArgThr: 4.757 ± 0.663
5.336ArgVal: 5.336 ± 0.695
1.864ArgTrp: 1.864 ± 0.4
1.993ArgTyr: 1.993 ± 0.379
0.0ArgXaa: 0.0 ± 0.0
Ser
7.586SerAla: 7.586 ± 0.806
0.321SerCys: 0.321 ± 0.133
3.343SerAsp: 3.343 ± 0.526
2.7SerGlu: 2.7 ± 0.447
1.736SerPhe: 1.736 ± 0.362
5.464SerGly: 5.464 ± 0.69
0.45SerHis: 0.45 ± 0.204
2.957SerIle: 2.957 ± 0.371
1.543SerLys: 1.543 ± 0.3
4.5SerLeu: 4.5 ± 0.55
0.836SerMet: 0.836 ± 0.228
1.221SerAsn: 1.221 ± 0.276
3.536SerPro: 3.536 ± 0.485
2.057SerGln: 2.057 ± 0.354
3.857SerArg: 3.857 ± 0.484
3.022SerSer: 3.022 ± 0.52
3.857SerThr: 3.857 ± 0.474
3.214SerVal: 3.214 ± 0.431
1.543SerTrp: 1.543 ± 0.309
1.221SerTyr: 1.221 ± 0.299
0.0SerXaa: 0.0 ± 0.0
Thr
9.707ThrAla: 9.707 ± 1.557
0.643ThrCys: 0.643 ± 0.18
4.822ThrAsp: 4.822 ± 0.441
2.764ThrGlu: 2.764 ± 0.454
1.414ThrPhe: 1.414 ± 0.321
6.622ThrGly: 6.622 ± 0.705
1.221ThrHis: 1.221 ± 0.248
4.243ThrIle: 4.243 ± 0.516
1.864ThrLys: 1.864 ± 0.369
5.722ThrLeu: 5.722 ± 0.546
0.707ThrMet: 0.707 ± 0.223
1.479ThrAsn: 1.479 ± 0.376
4.114ThrPro: 4.114 ± 0.497
2.25ThrGln: 2.25 ± 0.291
4.372ThrArg: 4.372 ± 0.523
4.114ThrSer: 4.114 ± 0.569
5.529ThrThr: 5.529 ± 0.801
5.4ThrVal: 5.4 ± 0.532
1.414ThrTrp: 1.414 ± 0.328
1.221ThrTyr: 1.221 ± 0.305
0.0ThrXaa: 0.0 ± 0.0
Val
8.357ValAla: 8.357 ± 0.75
0.707ValCys: 0.707 ± 0.241
6.3ValAsp: 6.3 ± 0.625
3.986ValGlu: 3.986 ± 0.45
2.572ValPhe: 2.572 ± 0.452
5.336ValGly: 5.336 ± 0.631
1.157ValHis: 1.157 ± 0.33
3.022ValIle: 3.022 ± 0.496
1.993ValLys: 1.993 ± 0.379
4.307ValLeu: 4.307 ± 0.578
1.093ValMet: 1.093 ± 0.295
1.671ValAsn: 1.671 ± 0.341
4.564ValPro: 4.564 ± 0.544
2.507ValGln: 2.507 ± 0.434
6.043ValArg: 6.043 ± 0.645
3.922ValSer: 3.922 ± 0.466
5.657ValThr: 5.657 ± 0.694
5.4ValVal: 5.4 ± 0.604
0.9ValTrp: 0.9 ± 0.254
1.157ValTyr: 1.157 ± 0.326
0.0ValXaa: 0.0 ± 0.0
Trp
1.929TrpAla: 1.929 ± 0.311
0.257TrpCys: 0.257 ± 0.123
1.093TrpAsp: 1.093 ± 0.325
1.093TrpGlu: 1.093 ± 0.215
0.707TrpPhe: 0.707 ± 0.261
1.414TrpGly: 1.414 ± 0.282
0.257TrpHis: 0.257 ± 0.126
0.45TrpIle: 0.45 ± 0.148
0.707TrpLys: 0.707 ± 0.329
2.314TrpLeu: 2.314 ± 0.346
0.514TrpMet: 0.514 ± 0.189
0.45TrpAsn: 0.45 ± 0.173
1.157TrpPro: 1.157 ± 0.281
0.964TrpGln: 0.964 ± 0.22
1.736TrpArg: 1.736 ± 0.319
1.221TrpSer: 1.221 ± 0.246
1.093TrpThr: 1.093 ± 0.271
1.479TrpVal: 1.479 ± 0.317
0.514TrpTrp: 0.514 ± 0.147
0.257TrpTyr: 0.257 ± 0.139
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.636TyrAla: 2.636 ± 0.397
0.257TyrCys: 0.257 ± 0.123
1.221TyrAsp: 1.221 ± 0.281
1.607TyrGlu: 1.607 ± 0.322
0.514TyrPhe: 0.514 ± 0.172
1.993TyrGly: 1.993 ± 0.346
0.771TyrHis: 0.771 ± 0.278
0.771TyrIle: 0.771 ± 0.194
0.643TyrLys: 0.643 ± 0.212
1.543TyrLeu: 1.543 ± 0.228
0.321TyrMet: 0.321 ± 0.16
0.707TyrAsn: 0.707 ± 0.19
1.286TyrPro: 1.286 ± 0.292
0.964TyrGln: 0.964 ± 0.221
1.286TyrArg: 1.286 ± 0.233
1.286TyrSer: 1.286 ± 0.228
1.607TyrThr: 1.607 ± 0.296
2.057TyrVal: 2.057 ± 0.348
0.514TyrTrp: 0.514 ± 0.206
0.193TyrTyr: 0.193 ± 0.108
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (15556 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski