Vibrio genomosp. F6 str. FF-238

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio; Vibrio genomosp. F6

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3650 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E5D724|A0A1E5D724_9VIBR ATP-binding protein Uup OS=Vibrio genomosp. F6 str. FF-238 OX=1191298 GN=uup PE=3 SV=1
MM1 pKa = 7.61KK2 pKa = 8.94KK3 pKa = 8.34TLVALAIAAVSTSAFAAEE21 pKa = 4.38TSSQNAKK28 pKa = 10.52PMFEE32 pKa = 4.62FDD34 pKa = 4.95PMHH37 pKa = 7.14KK38 pKa = 10.44DD39 pKa = 3.1QFSVSGAWGVGGYY52 pKa = 10.7YY53 pKa = 9.42DD54 pKa = 3.89TEE56 pKa = 4.01TDD58 pKa = 3.85AVYY61 pKa = 10.98DD62 pKa = 3.81DD63 pKa = 4.08WATGLTLAVNYY74 pKa = 9.97QNNRR78 pKa = 11.84FLGYY82 pKa = 10.4VEE84 pKa = 5.2VDD86 pKa = 3.15LEE88 pKa = 4.45MNYY91 pKa = 8.83STDD94 pKa = 3.3EE95 pKa = 4.16SKK97 pKa = 11.25KK98 pKa = 10.55DD99 pKa = 3.43IQSGPGTDD107 pKa = 5.39LDD109 pKa = 4.03KK110 pKa = 11.42AWIGFEE116 pKa = 3.95TGAGVLSFGWEE127 pKa = 3.66NDD129 pKa = 3.39TALDD133 pKa = 3.87KK134 pKa = 11.56VDD136 pKa = 3.69GKK138 pKa = 11.59GDD140 pKa = 3.32NTYY143 pKa = 11.1EE144 pKa = 4.3FGASAGDD151 pKa = 3.58ASDD154 pKa = 3.95GYY156 pKa = 11.49NVIKK160 pKa = 10.44FQGATSGIAYY170 pKa = 8.33GISMSEE176 pKa = 3.96TSDD179 pKa = 3.39DD180 pKa = 3.57RR181 pKa = 11.84TEE183 pKa = 4.5ADD185 pKa = 3.08SAYY188 pKa = 10.33NGYY191 pKa = 10.42VGLEE195 pKa = 3.43QDD197 pKa = 4.02TFNLYY202 pKa = 10.56AGYY205 pKa = 7.57EE206 pKa = 4.09TRR208 pKa = 11.84ADD210 pKa = 3.12ADD212 pKa = 3.99YY213 pKa = 11.57NVISVSGNVNLGEE226 pKa = 4.15LTLGANVWSNEE237 pKa = 3.76GADD240 pKa = 3.66DD241 pKa = 4.7VADD244 pKa = 3.39NTTNLKK250 pKa = 10.36EE251 pKa = 3.44IGYY254 pKa = 8.74YY255 pKa = 10.4VSGAYY260 pKa = 8.78PVSEE264 pKa = 4.62ALTLAAGYY272 pKa = 10.7AGGTVEE278 pKa = 5.16EE279 pKa = 5.33DD280 pKa = 2.78ATADD284 pKa = 2.99IDD286 pKa = 3.72ASYY289 pKa = 11.17FNVAAMYY296 pKa = 9.67TMSDD300 pKa = 3.54RR301 pKa = 11.84VDD303 pKa = 3.11MGIDD307 pKa = 2.99IRR309 pKa = 11.84RR310 pKa = 11.84DD311 pKa = 3.09LDD313 pKa = 3.67VPAGNDD319 pKa = 2.9EE320 pKa = 4.09EE321 pKa = 5.26TYY323 pKa = 10.75VFAAAFYY330 pKa = 10.91SFF332 pKa = 4.34

Molecular weight: 35.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E5CYD5|A0A1E5CYD5_9VIBR ABC transporter permease OS=Vibrio genomosp. F6 str. FF-238 OX=1191298 GN=A130_00565 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.5RR3 pKa = 11.84TFQPTVLKK11 pKa = 10.35RR12 pKa = 11.84KK13 pKa = 9.02RR14 pKa = 11.84SHH16 pKa = 6.15GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.44NGRR28 pKa = 11.84ATINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.74GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSKK44 pKa = 10.84

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3650

0

3650

1184933

30

2423

324.6

36.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.179 ± 0.04

1.047 ± 0.013

5.599 ± 0.038

6.432 ± 0.042

4.181 ± 0.031

6.667 ± 0.036

2.234 ± 0.021

6.631 ± 0.033

5.53 ± 0.038

10.278 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.697 ± 0.018

4.386 ± 0.029

3.781 ± 0.023

4.524 ± 0.037

4.36 ± 0.028

6.996 ± 0.04

5.351 ± 0.023

6.908 ± 0.033

1.208 ± 0.015

3.012 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski