Rodent papillomavirus
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H4MZ93|A0A2H4MZ93_9PAPI Replication protein E1 OS=Rodent papillomavirus OX=2050020 GN=E1 PE=3 SV=1
MM1 pKa = 7.25 VSATRR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 VKK10 pKa = 10.34 RR11 pKa = 11.84 DD12 pKa = 3.07 SASNLYY18 pKa = 9.65 RR19 pKa = 11.84 QCQVTGNCPPDD30 pKa = 3.41 VANKK34 pKa = 10.19 IEE36 pKa = 4.27 GNTLADD42 pKa = 3.59 KK43 pKa = 10.53 LLKK46 pKa = 10.12 IFSSIIYY53 pKa = 10.17 LGGLGIGTGRR63 pKa = 11.84 GTGEE67 pKa = 4.0 TYY69 pKa = 10.56 GYY71 pKa = 11.24 GPINPGGGRR80 pKa = 11.84 ITGTGTVMRR89 pKa = 11.84 PGVVVEE95 pKa = 4.83 PIGPAEE101 pKa = 4.09 IVPVDD106 pKa = 3.64 SVGPGEE112 pKa = 4.24 SSIVPLLEE120 pKa = 4.05 ATPDD124 pKa = 3.45 VPINGGPEE132 pKa = 3.87 VPPAGPDD139 pKa = 3.14 TSTVDD144 pKa = 3.23 VTSNIDD150 pKa = 3.32 PVSDD154 pKa = 3.75 VNVSGGTTITNTDD167 pKa = 3.28 SAVIDD172 pKa = 3.9 VQPAPSGPRR181 pKa = 11.84 RR182 pKa = 11.84 VTVTRR187 pKa = 11.84 SEE189 pKa = 4.53 HH190 pKa = 6.01 NNPAYY195 pKa = 10.68 VSVSAPPQGLGEE207 pKa = 4.39 GGGFLLAGSEE217 pKa = 4.26 SSGLIASTHH226 pKa = 5.62 EE227 pKa = 3.93 LDD229 pKa = 3.42 AAVIIGGRR237 pKa = 11.84 PPPEE241 pKa = 4.2 TIFEE245 pKa = 4.98 DD246 pKa = 3.55 IEE248 pKa = 5.74 LDD250 pKa = 3.65 TFSATGGRR258 pKa = 11.84 SEE260 pKa = 4.46 FDD262 pKa = 2.76 IEE264 pKa = 4.33 EE265 pKa = 4.99 PYY267 pKa = 11.15 SSTPRR272 pKa = 11.84 GGPLDD277 pKa = 3.53 RR278 pKa = 11.84 AVTRR282 pKa = 11.84 FRR284 pKa = 11.84 DD285 pKa = 3.77 LYY287 pKa = 10.26 NRR289 pKa = 11.84 RR290 pKa = 11.84 VQQVRR295 pKa = 11.84 VSDD298 pKa = 3.99 PEE300 pKa = 4.06 QFLSAPARR308 pKa = 11.84 AVTFEE313 pKa = 4.09 NPAFDD318 pKa = 4.28 SGDD321 pKa = 2.85 ISLIFEE327 pKa = 3.99 QDD329 pKa = 3.3 VQAVQATPDD338 pKa = 4.15 PDD340 pKa = 3.61 FMDD343 pKa = 5.15 IIRR346 pKa = 11.84 LGRR349 pKa = 11.84 PRR351 pKa = 11.84 LSEE354 pKa = 3.66 TADD357 pKa = 3.3 RR358 pKa = 11.84 SVRR361 pKa = 11.84 VSRR364 pKa = 11.84 LGQRR368 pKa = 11.84 GTIRR372 pKa = 11.84 TRR374 pKa = 11.84 SGLQIGGRR382 pKa = 11.84 VHH384 pKa = 7.08 YY385 pKa = 8.91 YY386 pKa = 10.22 TDD388 pKa = 4.32 LSPIPRR394 pKa = 11.84 DD395 pKa = 3.32 IEE397 pKa = 4.19 LSTLGEE403 pKa = 4.16 ASGEE407 pKa = 3.88 ADD409 pKa = 3.43 LVDD412 pKa = 5.22 GIGEE416 pKa = 4.27 SSVIDD421 pKa = 3.81 AGSVADD427 pKa = 3.94 EE428 pKa = 4.43 SMLIGEE434 pKa = 4.8 GLGAPLDD441 pKa = 4.32 DD442 pKa = 5.92 LDD444 pKa = 5.63 LISDD448 pKa = 4.4 GSSVDD453 pKa = 4.12 FSSTRR458 pKa = 11.84 LEE460 pKa = 4.16 FSFLTGSSNITLPEE474 pKa = 3.58 QVTNVFGPGLYY485 pKa = 10.49 VPDD488 pKa = 4.51 IGTGTHH494 pKa = 7.08 IIHH497 pKa = 6.3 PQVVGEE503 pKa = 4.41 VEE505 pKa = 4.72 LEE507 pKa = 3.95 DD508 pKa = 4.12 SFPRR512 pKa = 11.84 DD513 pKa = 3.67 LFPGTEE519 pKa = 3.58 IVFIPEE525 pKa = 3.98 DD526 pKa = 3.82 SIDD529 pKa = 4.14 FYY531 pKa = 11.3 LHH533 pKa = 6.17 PSLRR537 pKa = 11.84 RR538 pKa = 11.84 RR539 pKa = 11.84 KK540 pKa = 8.82 RR541 pKa = 11.84 KK542 pKa = 9.74 YY543 pKa = 9.81 FVRR546 pKa = 4.99
Molecular weight: 57.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.606
IPC2_protein 4.495
IPC_protein 4.469
Toseland 4.291
ProMoST 4.584
Dawson 4.418
Bjellqvist 4.571
Wikipedia 4.304
Rodwell 4.291
Grimsley 4.202
Solomon 4.418
Lehninger 4.38
Nozaki 4.533
DTASelect 4.724
Thurlkill 4.304
EMBOSS 4.329
Sillero 4.584
Patrickios 4.062
IPC_peptide 4.418
IPC2_peptide 4.571
IPC2.peptide.svr19 4.519
Protein with the highest isoelectric point:
>tr|A0A2H4MY42|A0A2H4MY42_9PAPI Minor capsid protein L2 OS=Rodent papillomavirus OX=2050020 GN=L2 PE=3 SV=1
MM1 pKa = 7.03 TLKK4 pKa = 10.83 GYY6 pKa = 7.94 TLLDD10 pKa = 3.44 LCRR13 pKa = 11.84 YY14 pKa = 9.18 YY15 pKa = 10.94 DD16 pKa = 3.59 VSPTSFPLDD25 pKa = 3.56 CTFCDD30 pKa = 4.43 CVLDD34 pKa = 4.29 DD35 pKa = 4.03 VDD37 pKa = 4.1 RR38 pKa = 11.84 ASFAASKK45 pKa = 10.65 LKK47 pKa = 10.8 VVVRR51 pKa = 11.84 NLQYY55 pKa = 10.91 KK56 pKa = 7.81 GACIKK61 pKa = 10.37 CRR63 pKa = 11.84 RR64 pKa = 11.84 ALAAEE69 pKa = 4.39 EE70 pKa = 4.07 KK71 pKa = 10.28 FKK73 pKa = 11.44 YY74 pKa = 9.54 LVCVGEE80 pKa = 4.41 ADD82 pKa = 4.71 LVEE85 pKa = 4.42 AMCGTGIVHH94 pKa = 6.67 CSVRR98 pKa = 11.84 CVSCLALLSASEE110 pKa = 4.1 KK111 pKa = 10.62 LVAKK115 pKa = 9.83 GGCQPFYY122 pKa = 10.77 LVRR125 pKa = 11.84 HH126 pKa = 6.3 LWRR129 pKa = 11.84 AQCRR133 pKa = 11.84 HH134 pKa = 5.89 CRR136 pKa = 11.84 TPGCC140 pKa = 4.15
Molecular weight: 15.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.468
IPC2_protein 7.571
IPC_protein 7.366
Toseland 6.781
ProMoST 8.009
Dawson 8.068
Bjellqvist 8.624
Wikipedia 7.966
Rodwell 8.083
Grimsley 6.751
Solomon 8.112
Lehninger 8.141
Nozaki 8.99
DTASelect 8.229
Thurlkill 8.273
EMBOSS 8.273
Sillero 8.682
Patrickios 1.354
IPC_peptide 8.112
IPC2_peptide 8.126
IPC2.peptide.svr19 8.064
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2287
101
602
381.2
42.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.466 ± 0.687
2.405 ± 0.975
6.865 ± 0.26
5.509 ± 0.8
4.547 ± 0.579
8.177 ± 0.93
2.055 ± 0.341
3.979 ± 0.857
4.285 ± 0.886
8.483 ± 0.645
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.53 ± 0.228
3.804 ± 0.69
6.296 ± 1.047
4.066 ± 0.485
6.777 ± 0.479
7.127 ± 0.724
7.258 ± 0.51
7.215 ± 0.585
1.181 ± 0.324
2.973 ± 0.302
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here