Arenimonas sp. 3729k
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2417 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B2ZAZ9|A0A5B2ZAZ9_9GAMM Succinate dehydrogenase cytochrome b556 subunit OS=Arenimonas sp. 3729k OX=2608255 GN=sdhC PE=3 SV=1
MM1 pKa = 7.66 SSTDD5 pKa = 2.84 TATAYY10 pKa = 7.52 RR11 pKa = 11.84 TWMCVVCGFTYY22 pKa = 10.71 DD23 pKa = 3.42 EE24 pKa = 4.41 AAGLPEE30 pKa = 4.46 EE31 pKa = 5.49 GIAPGTRR38 pKa = 11.84 WEE40 pKa = 5.59 DD41 pKa = 3.48 IPDD44 pKa = 3.5 DD45 pKa = 4.38 WVCPDD50 pKa = 4.01 CGVTKK55 pKa = 10.46 EE56 pKa = 4.2 DD57 pKa = 3.92 FEE59 pKa = 4.61 MVPVAA64 pKa = 5.08
Molecular weight: 7.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.84
IPC2_protein 3.681
IPC_protein 3.579
Toseland 3.401
ProMoST 3.783
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.554
Rodwell 3.427
Grimsley 3.325
Solomon 3.554
Lehninger 3.503
Nozaki 3.732
DTASelect 3.897
Thurlkill 3.478
EMBOSS 3.554
Sillero 3.719
Patrickios 0.299
IPC_peptide 3.541
IPC2_peptide 3.681
IPC2.peptide.svr19 3.691
Protein with the highest isoelectric point:
>tr|A0A5B2ZDW6|A0A5B2ZDW6_9GAMM 50S ribosomal protein L30 OS=Arenimonas sp. 3729k OX=2608255 GN=rpmD PE=3 SV=1
MM1 pKa = 7.05 ATKK4 pKa = 10.34 KK5 pKa = 10.11 AAKK8 pKa = 10.0 KK9 pKa = 8.64 PAKK12 pKa = 9.72 KK13 pKa = 9.81 AAKK16 pKa = 9.99 KK17 pKa = 9.87 SVKK20 pKa = 9.86 KK21 pKa = 10.33 AAAKK25 pKa = 9.71 KK26 pKa = 10.04 AVRR29 pKa = 11.84 KK30 pKa = 8.8 VAKK33 pKa = 9.5 KK34 pKa = 10.31 AKK36 pKa = 9.64 KK37 pKa = 9.64 AVKK40 pKa = 10.0 KK41 pKa = 9.58 VAKK44 pKa = 9.94 KK45 pKa = 9.19 VAKK48 pKa = 10.07 KK49 pKa = 9.17 ATRR52 pKa = 11.84 KK53 pKa = 8.32 PARR56 pKa = 11.84 KK57 pKa = 8.79 AAKK60 pKa = 9.82 KK61 pKa = 9.91 KK62 pKa = 9.53 AAKK65 pKa = 9.82 KK66 pKa = 8.84 ATRR69 pKa = 11.84 KK70 pKa = 8.34 PARR73 pKa = 11.84 KK74 pKa = 8.75 AAKK77 pKa = 9.96 KK78 pKa = 10.04 KK79 pKa = 8.76 VAKK82 pKa = 10.07 KK83 pKa = 9.11 ATRR86 pKa = 11.84 KK87 pKa = 8.32 PARR90 pKa = 11.84 KK91 pKa = 8.75 AAKK94 pKa = 9.96 KK95 pKa = 10.11 KK96 pKa = 8.77 VAKK99 pKa = 10.01 KK100 pKa = 10.08 AARR103 pKa = 11.84 KK104 pKa = 7.3 PAKK107 pKa = 10.07 KK108 pKa = 9.91 AVKK111 pKa = 9.89 KK112 pKa = 10.03 AAKK115 pKa = 10.03 KK116 pKa = 8.68 PAKK119 pKa = 9.74 KK120 pKa = 9.92 KK121 pKa = 9.95 AARR124 pKa = 11.84 KK125 pKa = 8.24 PAAKK129 pKa = 9.81 KK130 pKa = 8.32 PAPMMPAAPVAPALL144 pKa = 4.04
Molecular weight: 15.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.374
IPC_protein 12.749
Toseland 12.983
ProMoST 13.408
Dawson 12.998
Bjellqvist 12.939
Wikipedia 13.422
Rodwell 13.32
Grimsley 13.027
Solomon 13.437
Lehninger 13.349
Nozaki 12.983
DTASelect 12.939
Thurlkill 12.983
EMBOSS 13.466
Sillero 12.983
Patrickios 13.013
IPC_peptide 13.437
IPC2_peptide 12.398
IPC2.peptide.svr19 9.043
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2417
0
2417
808156
36
2205
334.4
36.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.429 ± 0.074
0.852 ± 0.015
5.453 ± 0.033
6.2 ± 0.046
3.285 ± 0.03
8.981 ± 0.045
2.144 ± 0.025
3.65 ± 0.034
2.325 ± 0.043
11.726 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.078 ± 0.025
2.054 ± 0.03
5.763 ± 0.038
3.269 ± 0.029
8.87 ± 0.057
4.535 ± 0.035
4.102 ± 0.036
7.492 ± 0.044
1.601 ± 0.024
2.192 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here