Streptococcus phage EJ-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q708Q5|Q708Q5_9CAUD Uncharacterized protein OS=Streptococcus phage EJ-1 OX=12402 PE=4 SV=1
MM1 pKa = 8.03RR2 pKa = 11.84MFDD5 pKa = 3.7YY6 pKa = 10.83DD7 pKa = 4.35RR8 pKa = 11.84DD9 pKa = 3.51IMQPPEE15 pKa = 3.98EE16 pKa = 4.68RR17 pKa = 11.84EE18 pKa = 3.79EE19 pKa = 4.45LDD21 pKa = 3.34PSEE24 pKa = 4.53YY25 pKa = 9.9IYY27 pKa = 10.75VGCGQYY33 pKa = 10.33RR34 pKa = 11.84YY35 pKa = 10.77VGDD38 pKa = 3.84EE39 pKa = 3.92VV40 pKa = 3.41

Molecular weight: 4.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q708M4|Q708M4_9CAUD Uncharacterized protein OS=Streptococcus phage EJ-1 OX=12402 PE=4 SV=1
MM1 pKa = 7.61SGGFDD6 pKa = 3.21YY7 pKa = 11.09RR8 pKa = 11.84SFAKK12 pKa = 10.33FANNFNRR19 pKa = 11.84NANHH23 pKa = 6.46AKK25 pKa = 9.35VDD27 pKa = 3.23RR28 pKa = 11.84FMRR31 pKa = 11.84QTLNYY36 pKa = 9.37EE37 pKa = 3.93GTEE40 pKa = 3.99LKK42 pKa = 10.86SKK44 pKa = 10.39VKK46 pKa = 10.06EE47 pKa = 3.88RR48 pKa = 11.84TPVGVYY54 pKa = 8.64TDD56 pKa = 3.18HH57 pKa = 6.61WVEE60 pKa = 4.09FTTKK64 pKa = 10.25DD65 pKa = 3.33GKK67 pKa = 9.88HH68 pKa = 4.21VKK70 pKa = 10.04FWASAHH76 pKa = 5.76GKK78 pKa = 9.61QGGTLQKK85 pKa = 9.74GWSKK89 pKa = 11.29SRR91 pKa = 11.84IEE93 pKa = 4.13VSGRR97 pKa = 11.84TYY99 pKa = 9.87KK100 pKa = 10.53QKK102 pKa = 10.84VYY104 pKa = 10.99NKK106 pKa = 9.57VYY108 pKa = 9.12YY109 pKa = 9.95APHH112 pKa = 6.49VEE114 pKa = 4.3YY115 pKa = 10.69GHH117 pKa = 5.95KK118 pKa = 8.85TVNGGFVPGQFFLHH132 pKa = 6.0KK133 pKa = 9.13TVEE136 pKa = 4.26DD137 pKa = 3.81TKK139 pKa = 11.42SDD141 pKa = 3.33MEE143 pKa = 3.97KK144 pKa = 10.28RR145 pKa = 11.84VRR147 pKa = 11.84DD148 pKa = 3.71KK149 pKa = 11.45YY150 pKa = 11.13DD151 pKa = 2.79GFMRR155 pKa = 11.84KK156 pKa = 8.24VVLGNGKK163 pKa = 9.57

Molecular weight: 18.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

13205

34

748

180.9

20.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.285 ± 0.472

0.75 ± 0.094

6.543 ± 0.217

8.201 ± 0.487

4.029 ± 0.176

5.884 ± 0.392

1.568 ± 0.139

6.725 ± 0.265

8.663 ± 0.35

8.701 ± 0.253

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.628 ± 0.218

5.445 ± 0.183

2.764 ± 0.184

3.885 ± 0.169

4.604 ± 0.23

5.498 ± 0.238

5.808 ± 0.25

6.808 ± 0.298

1.227 ± 0.132

3.983 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski