Amino acid dipepetide frequency for Streptococcus phage EJ-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.121AlaAla: 2.121 ± 0.546
0.682AlaCys: 0.682 ± 0.194
4.468AlaAsp: 4.468 ± 0.552
5.68AlaGlu: 5.68 ± 0.695
2.651AlaPhe: 2.651 ± 0.5
5.226AlaGly: 5.226 ± 0.797
0.682AlaHis: 0.682 ± 0.268
4.468AlaIle: 4.468 ± 0.536
4.847AlaLys: 4.847 ± 0.616
6.21AlaLeu: 6.21 ± 0.758
1.969AlaMet: 1.969 ± 0.519
3.484AlaAsn: 3.484 ± 0.492
1.59AlaPro: 1.59 ± 0.578
2.196AlaGln: 2.196 ± 0.398
2.348AlaArg: 2.348 ± 0.299
3.408AlaSer: 3.408 ± 0.401
4.241AlaThr: 4.241 ± 0.598
4.165AlaVal: 4.165 ± 0.765
0.606AlaTrp: 0.606 ± 0.199
1.818AlaTyr: 1.818 ± 0.334
0.0AlaXaa: 0.0 ± 0.0
Cys
0.151CysAla: 0.151 ± 0.134
0.0CysCys: 0.0 ± 0.0
0.303CysAsp: 0.303 ± 0.184
0.303CysGlu: 0.303 ± 0.116
0.303CysPhe: 0.303 ± 0.142
0.53CysGly: 0.53 ± 0.18
0.151CysHis: 0.151 ± 0.124
0.454CysIle: 0.454 ± 0.192
0.606CysLys: 0.606 ± 0.221
0.53CysLeu: 0.53 ± 0.179
0.0CysMet: 0.0 ± 0.0
0.379CysAsn: 0.379 ± 0.179
0.53CysPro: 0.53 ± 0.184
0.303CysGln: 0.303 ± 0.149
0.606CysArg: 0.606 ± 0.199
0.606CysSer: 0.606 ± 0.218
0.454CysThr: 0.454 ± 0.182
0.757CysVal: 0.757 ± 0.248
0.076CysTrp: 0.076 ± 0.095
0.454CysTyr: 0.454 ± 0.174
0.0CysXaa: 0.0 ± 0.0
Asp
3.408AspAla: 3.408 ± 0.393
0.303AspCys: 0.303 ± 0.161
4.09AspAsp: 4.09 ± 0.434
5.15AspGlu: 5.15 ± 0.536
2.651AspPhe: 2.651 ± 0.404
4.998AspGly: 4.998 ± 0.592
0.606AspHis: 0.606 ± 0.168
5.453AspIle: 5.453 ± 0.58
5.226AspLys: 5.226 ± 0.663
6.135AspLeu: 6.135 ± 0.564
2.651AspMet: 2.651 ± 0.473
3.635AspAsn: 3.635 ± 0.508
1.59AspPro: 1.59 ± 0.327
1.363AspGln: 1.363 ± 0.321
2.424AspArg: 2.424 ± 0.412
4.014AspSer: 4.014 ± 0.587
3.484AspThr: 3.484 ± 0.455
3.787AspVal: 3.787 ± 0.555
0.833AspTrp: 0.833 ± 0.279
3.635AspTyr: 3.635 ± 0.56
0.0AspXaa: 0.0 ± 0.0
Glu
4.09GluAla: 4.09 ± 0.513
0.606GluCys: 0.606 ± 0.195
5.453GluAsp: 5.453 ± 0.736
6.968GluGlu: 6.968 ± 1.159
3.862GluPhe: 3.862 ± 0.474
2.802GluGly: 2.802 ± 0.478
0.833GluHis: 0.833 ± 0.237
5.529GluIle: 5.529 ± 0.639
7.271GluLys: 7.271 ± 1.035
9.012GluLeu: 9.012 ± 0.968
2.878GluMet: 2.878 ± 0.417
4.393GluAsn: 4.393 ± 0.594
2.196GluPro: 2.196 ± 0.522
3.635GluGln: 3.635 ± 0.46
4.165GluArg: 4.165 ± 0.685
4.317GluSer: 4.317 ± 0.62
4.771GluThr: 4.771 ± 0.7
5.756GluVal: 5.756 ± 0.676
0.909GluTrp: 0.909 ± 0.261
2.575GluTyr: 2.575 ± 0.412
0.0GluXaa: 0.0 ± 0.0
Phe
2.878PheAla: 2.878 ± 0.559
0.379PheCys: 0.379 ± 0.155
2.575PheAsp: 2.575 ± 0.375
3.029PheGlu: 3.029 ± 0.524
1.742PhePhe: 1.742 ± 0.401
2.651PheGly: 2.651 ± 0.337
0.303PheHis: 0.303 ± 0.144
3.257PheIle: 3.257 ± 0.456
4.014PheLys: 4.014 ± 0.579
2.726PheLeu: 2.726 ± 0.462
1.212PheMet: 1.212 ± 0.355
2.121PheAsn: 2.121 ± 0.355
0.833PhePro: 0.833 ± 0.292
0.909PheGln: 0.909 ± 0.195
1.818PheArg: 1.818 ± 0.389
2.272PheSer: 2.272 ± 0.35
2.348PheThr: 2.348 ± 0.377
3.635PheVal: 3.635 ± 0.54
0.303PheTrp: 0.303 ± 0.132
1.287PheTyr: 1.287 ± 0.229
0.0PheXaa: 0.0 ± 0.0
Gly
3.635GlyAla: 3.635 ± 0.598
0.303GlyCys: 0.303 ± 0.146
3.862GlyAsp: 3.862 ± 0.54
3.787GlyGlu: 3.787 ± 0.507
2.878GlyPhe: 2.878 ± 0.509
2.726GlyGly: 2.726 ± 0.577
1.06GlyHis: 1.06 ± 0.222
4.62GlyIle: 4.62 ± 0.714
5.377GlyLys: 5.377 ± 0.7
4.165GlyLeu: 4.165 ± 0.564
1.287GlyMet: 1.287 ± 0.259
3.938GlyAsn: 3.938 ± 0.615
0.682GlyPro: 0.682 ± 0.216
2.726GlyGln: 2.726 ± 0.592
3.408GlyArg: 3.408 ± 0.568
2.651GlySer: 2.651 ± 0.436
3.257GlyThr: 3.257 ± 0.456
4.317GlyVal: 4.317 ± 0.742
1.439GlyTrp: 1.439 ± 0.509
2.726GlyTyr: 2.726 ± 0.524
0.0GlyXaa: 0.0 ± 0.0
His
0.833HisAla: 0.833 ± 0.285
0.379HisCys: 0.379 ± 0.171
0.53HisAsp: 0.53 ± 0.207
1.136HisGlu: 1.136 ± 0.282
0.53HisPhe: 0.53 ± 0.161
1.287HisGly: 1.287 ± 0.349
0.076HisHis: 0.076 ± 0.075
1.136HisIle: 1.136 ± 0.334
1.136HisLys: 1.136 ± 0.279
1.742HisLeu: 1.742 ± 0.338
0.151HisMet: 0.151 ± 0.104
0.682HisAsn: 0.682 ± 0.305
0.757HisPro: 0.757 ± 0.209
0.833HisGln: 0.833 ± 0.287
0.682HisArg: 0.682 ± 0.241
0.833HisSer: 0.833 ± 0.288
0.682HisThr: 0.682 ± 0.204
1.06HisVal: 1.06 ± 0.285
0.227HisTrp: 0.227 ± 0.119
0.985HisTyr: 0.985 ± 0.31
0.0HisXaa: 0.0 ± 0.0
Ile
5.15IleAla: 5.15 ± 0.773
0.682IleCys: 0.682 ± 0.234
5.377IleAsp: 5.377 ± 0.664
5.68IleGlu: 5.68 ± 0.682
2.196IlePhe: 2.196 ± 0.346
4.847IleGly: 4.847 ± 0.769
1.06IleHis: 1.06 ± 0.283
3.257IleIle: 3.257 ± 0.505
6.513IleLys: 6.513 ± 0.646
5.756IleLeu: 5.756 ± 0.724
1.287IleMet: 1.287 ± 0.308
2.802IleAsn: 2.802 ± 0.613
2.575IlePro: 2.575 ± 0.507
2.954IleGln: 2.954 ± 0.485
2.954IleArg: 2.954 ± 0.57
3.711IleSer: 3.711 ± 0.659
3.181IleThr: 3.181 ± 0.468
3.635IleVal: 3.635 ± 0.49
0.833IleTrp: 0.833 ± 0.286
2.802IleTyr: 2.802 ± 0.467
0.0IleXaa: 0.0 ± 0.0
Lys
6.286LysAla: 6.286 ± 0.812
0.151LysCys: 0.151 ± 0.111
5.604LysAsp: 5.604 ± 0.653
9.391LysGlu: 9.391 ± 1.447
1.818LysPhe: 1.818 ± 0.295
3.635LysGly: 3.635 ± 0.507
1.515LysHis: 1.515 ± 0.308
5.226LysIle: 5.226 ± 0.514
8.179LysLys: 8.179 ± 1.003
7.346LysLeu: 7.346 ± 0.558
2.196LysMet: 2.196 ± 0.537
5.529LysAsn: 5.529 ± 0.659
1.969LysPro: 1.969 ± 0.351
4.62LysGln: 4.62 ± 0.589
4.165LysArg: 4.165 ± 0.657
3.938LysSer: 3.938 ± 0.528
4.317LysThr: 4.317 ± 0.594
6.135LysVal: 6.135 ± 0.739
1.06LysTrp: 1.06 ± 0.326
3.56LysTyr: 3.56 ± 0.519
0.0LysXaa: 0.0 ± 0.0
Leu
5.907LeuAla: 5.907 ± 0.693
0.454LeuCys: 0.454 ± 0.163
6.74LeuAsp: 6.74 ± 0.672
8.558LeuGlu: 8.558 ± 0.921
2.348LeuPhe: 2.348 ± 0.526
6.513LeuGly: 6.513 ± 0.607
1.363LeuHis: 1.363 ± 0.309
6.21LeuIle: 6.21 ± 0.699
8.407LeuLys: 8.407 ± 0.883
7.346LeuLeu: 7.346 ± 0.68
1.893LeuMet: 1.893 ± 0.391
4.62LeuAsn: 4.62 ± 0.5
2.348LeuPro: 2.348 ± 0.387
2.802LeuGln: 2.802 ± 0.46
4.014LeuArg: 4.014 ± 0.465
4.317LeuSer: 4.317 ± 0.611
5.377LeuThr: 5.377 ± 0.569
4.847LeuVal: 4.847 ± 0.658
0.53LeuTrp: 0.53 ± 0.255
2.424LeuTyr: 2.424 ± 0.377
0.0LeuXaa: 0.0 ± 0.0
Met
2.726MetAla: 2.726 ± 0.64
0.379MetCys: 0.379 ± 0.166
1.969MetAsp: 1.969 ± 0.406
1.742MetGlu: 1.742 ± 0.363
0.985MetPhe: 0.985 ± 0.315
0.909MetGly: 0.909 ± 0.293
0.454MetHis: 0.454 ± 0.201
2.196MetIle: 2.196 ± 0.418
2.499MetLys: 2.499 ± 0.34
1.969MetLeu: 1.969 ± 0.41
0.833MetMet: 0.833 ± 0.318
1.287MetAsn: 1.287 ± 0.268
0.53MetPro: 0.53 ± 0.209
1.06MetGln: 1.06 ± 0.23
1.136MetArg: 1.136 ± 0.282
1.287MetSer: 1.287 ± 0.272
2.499MetThr: 2.499 ± 0.495
1.287MetVal: 1.287 ± 0.31
0.303MetTrp: 0.303 ± 0.148
0.227MetTyr: 0.227 ± 0.117
0.0MetXaa: 0.0 ± 0.0
Asn
3.408AsnAla: 3.408 ± 0.488
0.606AsnCys: 0.606 ± 0.263
3.862AsnAsp: 3.862 ± 0.545
4.09AsnGlu: 4.09 ± 0.625
2.045AsnPhe: 2.045 ± 0.32
3.56AsnGly: 3.56 ± 0.474
1.287AsnHis: 1.287 ± 0.326
3.257AsnIle: 3.257 ± 0.515
3.408AsnLys: 3.408 ± 0.554
4.468AsnLeu: 4.468 ± 0.575
1.363AsnMet: 1.363 ± 0.312
2.878AsnAsn: 2.878 ± 0.501
2.045AsnPro: 2.045 ± 0.48
2.045AsnGln: 2.045 ± 0.495
2.499AsnArg: 2.499 ± 0.434
2.954AsnSer: 2.954 ± 0.474
2.878AsnThr: 2.878 ± 0.605
4.544AsnVal: 4.544 ± 0.577
1.212AsnTrp: 1.212 ± 0.283
2.045AsnTyr: 2.045 ± 0.4
0.0AsnXaa: 0.0 ± 0.0
Pro
1.666ProAla: 1.666 ± 0.364
0.454ProCys: 0.454 ± 0.178
1.893ProAsp: 1.893 ± 0.399
1.742ProGlu: 1.742 ± 0.404
1.363ProPhe: 1.363 ± 0.286
0.833ProGly: 0.833 ± 0.303
0.379ProHis: 0.379 ± 0.174
2.045ProIle: 2.045 ± 0.426
2.651ProLys: 2.651 ± 0.44
1.666ProLeu: 1.666 ± 0.357
0.454ProMet: 0.454 ± 0.191
1.212ProAsn: 1.212 ± 0.312
0.682ProPro: 0.682 ± 0.252
1.363ProGln: 1.363 ± 0.297
0.757ProArg: 0.757 ± 0.282
2.121ProSer: 2.121 ± 0.315
2.045ProThr: 2.045 ± 0.44
2.272ProVal: 2.272 ± 0.319
0.151ProTrp: 0.151 ± 0.097
1.893ProTyr: 1.893 ± 0.362
0.0ProXaa: 0.0 ± 0.0
Gln
3.257GlnAla: 3.257 ± 0.611
0.227GlnCys: 0.227 ± 0.15
2.272GlnAsp: 2.272 ± 0.416
3.787GlnGlu: 3.787 ± 0.568
1.742GlnPhe: 1.742 ± 0.33
2.196GlnGly: 2.196 ± 0.429
0.379GlnHis: 0.379 ± 0.142
1.893GlnIle: 1.893 ± 0.34
3.56GlnLys: 3.56 ± 0.521
3.408GlnLeu: 3.408 ± 0.439
1.136GlnMet: 1.136 ± 0.265
1.818GlnAsn: 1.818 ± 0.368
1.287GlnPro: 1.287 ± 0.321
1.59GlnGln: 1.59 ± 0.285
1.515GlnArg: 1.515 ± 0.311
2.121GlnSer: 2.121 ± 0.364
2.121GlnThr: 2.121 ± 0.494
2.575GlnVal: 2.575 ± 0.425
0.53GlnTrp: 0.53 ± 0.195
1.439GlnTyr: 1.439 ± 0.311
0.0GlnXaa: 0.0 ± 0.0
Arg
2.726ArgAla: 2.726 ± 0.428
0.454ArgCys: 0.454 ± 0.185
2.272ArgAsp: 2.272 ± 0.481
4.09ArgGlu: 4.09 ± 0.623
1.893ArgPhe: 1.893 ± 0.416
1.893ArgGly: 1.893 ± 0.469
0.757ArgHis: 0.757 ± 0.232
3.105ArgIle: 3.105 ± 0.445
3.711ArgLys: 3.711 ± 0.504
4.317ArgLeu: 4.317 ± 0.509
1.439ArgMet: 1.439 ± 0.305
2.424ArgAsn: 2.424 ± 0.408
0.909ArgPro: 0.909 ± 0.272
2.499ArgGln: 2.499 ± 0.542
2.272ArgArg: 2.272 ± 0.405
2.348ArgSer: 2.348 ± 0.444
2.954ArgThr: 2.954 ± 0.398
3.105ArgVal: 3.105 ± 0.627
0.606ArgTrp: 0.606 ± 0.212
2.272ArgTyr: 2.272 ± 0.418
0.0ArgXaa: 0.0 ± 0.0
Ser
3.484SerAla: 3.484 ± 0.578
0.303SerCys: 0.303 ± 0.167
3.105SerAsp: 3.105 ± 0.403
3.56SerGlu: 3.56 ± 0.471
3.257SerPhe: 3.257 ± 0.534
4.014SerGly: 4.014 ± 0.523
1.212SerHis: 1.212 ± 0.284
3.862SerIle: 3.862 ± 0.476
4.696SerLys: 4.696 ± 0.582
4.771SerLeu: 4.771 ± 0.538
1.439SerMet: 1.439 ± 0.402
3.029SerAsn: 3.029 ± 0.488
1.59SerPro: 1.59 ± 0.383
1.893SerGln: 1.893 ± 0.399
2.575SerArg: 2.575 ± 0.432
3.105SerSer: 3.105 ± 0.583
3.257SerThr: 3.257 ± 0.467
3.257SerVal: 3.257 ± 0.623
0.303SerTrp: 0.303 ± 0.16
2.272SerTyr: 2.272 ± 0.44
0.0SerXaa: 0.0 ± 0.0
Thr
3.332ThrAla: 3.332 ± 0.539
0.303ThrCys: 0.303 ± 0.135
3.181ThrAsp: 3.181 ± 0.593
3.635ThrGlu: 3.635 ± 0.505
2.726ThrPhe: 2.726 ± 0.538
4.014ThrGly: 4.014 ± 0.814
1.136ThrHis: 1.136 ± 0.271
4.014ThrIle: 4.014 ± 0.473
4.165ThrLys: 4.165 ± 0.555
5.832ThrLeu: 5.832 ± 0.62
1.439ThrMet: 1.439 ± 0.285
3.408ThrAsn: 3.408 ± 0.445
2.348ThrPro: 2.348 ± 0.456
1.818ThrGln: 1.818 ± 0.437
2.045ThrArg: 2.045 ± 0.311
3.711ThrSer: 3.711 ± 0.508
3.711ThrThr: 3.711 ± 0.581
4.62ThrVal: 4.62 ± 0.702
0.606ThrTrp: 0.606 ± 0.254
2.045ThrTyr: 2.045 ± 0.303
0.0ThrXaa: 0.0 ± 0.0
Val
4.998ValAla: 4.998 ± 0.873
0.454ValCys: 0.454 ± 0.236
4.923ValAsp: 4.923 ± 0.524
5.68ValGlu: 5.68 ± 0.717
2.651ValPhe: 2.651 ± 0.505
3.787ValGly: 3.787 ± 0.812
1.212ValHis: 1.212 ± 0.321
3.56ValIle: 3.56 ± 0.499
5.983ValLys: 5.983 ± 0.707
5.68ValLeu: 5.68 ± 0.605
1.363ValMet: 1.363 ± 0.285
4.241ValAsn: 4.241 ± 0.479
2.121ValPro: 2.121 ± 0.409
2.045ValGln: 2.045 ± 0.35
3.029ValArg: 3.029 ± 0.493
4.771ValSer: 4.771 ± 0.574
4.09ValThr: 4.09 ± 0.575
4.165ValVal: 4.165 ± 0.708
0.833ValTrp: 0.833 ± 0.244
2.499ValTyr: 2.499 ± 0.451
0.0ValXaa: 0.0 ± 0.0
Trp
0.606TrpAla: 0.606 ± 0.18
0.0TrpCys: 0.0 ± 0.0
0.606TrpAsp: 0.606 ± 0.219
0.606TrpGlu: 0.606 ± 0.184
0.53TrpPhe: 0.53 ± 0.193
0.379TrpGly: 0.379 ± 0.174
0.227TrpHis: 0.227 ± 0.12
0.985TrpIle: 0.985 ± 0.273
1.515TrpLys: 1.515 ± 0.331
1.212TrpLeu: 1.212 ± 0.291
0.076TrpMet: 0.076 ± 0.09
0.682TrpAsn: 0.682 ± 0.191
0.151TrpPro: 0.151 ± 0.114
0.379TrpGln: 0.379 ± 0.18
0.682TrpArg: 0.682 ± 0.204
0.682TrpSer: 0.682 ± 0.22
0.757TrpThr: 0.757 ± 0.2
1.06TrpVal: 1.06 ± 0.216
0.303TrpTrp: 0.303 ± 0.17
0.833TrpTyr: 0.833 ± 0.471
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.196TyrAla: 2.196 ± 0.428
0.379TyrCys: 0.379 ± 0.144
2.348TyrAsp: 2.348 ± 0.414
3.408TyrGlu: 3.408 ± 0.467
2.121TyrPhe: 2.121 ± 0.483
2.045TyrGly: 2.045 ± 0.338
0.985TyrHis: 0.985 ± 0.275
2.726TyrIle: 2.726 ± 0.436
2.878TyrLys: 2.878 ± 0.482
2.726TyrLeu: 2.726 ± 0.524
1.136TyrMet: 1.136 ± 0.299
1.893TyrAsn: 1.893 ± 0.388
0.909TyrPro: 0.909 ± 0.301
1.818TyrGln: 1.818 ± 0.341
2.954TyrArg: 2.954 ± 0.512
1.818TyrSer: 1.818 ± 0.413
1.666TyrThr: 1.666 ± 0.397
3.105TyrVal: 3.105 ± 0.474
0.606TyrTrp: 0.606 ± 0.169
2.045TyrTyr: 2.045 ± 0.44
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 73 proteins (13205 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski