Riverside virus 1
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140D049|A0A140D049_9RHAB Glycoprotein OS=Riverside virus 1 OX=1803263 GN=G PE=4 SV=1
MM1 pKa = 7.51 AVNRR5 pKa = 11.84 PIKK8 pKa = 10.14 RR9 pKa = 11.84 ISDD12 pKa = 3.7 HH13 pKa = 7.19 PEE15 pKa = 3.52 LSPEE19 pKa = 4.14 VVHH22 pKa = 7.09 FDD24 pKa = 3.01 IGTDD28 pKa = 3.43 RR29 pKa = 11.84 PIGYY33 pKa = 8.68 PSAWFAQNVNDD44 pKa = 4.7 KK45 pKa = 8.82 PTIMMIPDD53 pKa = 3.75 ASVTRR58 pKa = 11.84 EE59 pKa = 3.86 QLHH62 pKa = 5.2 AAIRR66 pKa = 11.84 AGLLTGTVDD75 pKa = 2.74 HH76 pKa = 6.71 RR77 pKa = 11.84 FVVRR81 pKa = 11.84 FLYY84 pKa = 10.99 NEE86 pKa = 4.04 FNLVPGLLNSDD97 pKa = 2.69 WRR99 pKa = 11.84 SFGRR103 pKa = 11.84 NLGNRR108 pKa = 11.84 GEE110 pKa = 4.54 RR111 pKa = 11.84 IGPSSIIVPRR121 pKa = 11.84 EE122 pKa = 3.92 QEE124 pKa = 4.24 SVPALVAGNGLMDD137 pKa = 4.22 DD138 pKa = 4.12 QEE140 pKa = 4.33 VLRR143 pKa = 11.84 CIILICSVYY152 pKa = 10.07 RR153 pKa = 11.84 AGPISRR159 pKa = 11.84 VDD161 pKa = 3.3 YY162 pKa = 10.52 RR163 pKa = 11.84 DD164 pKa = 3.28 QVLEE168 pKa = 4.04 NVGNLLTPLGMDD180 pKa = 3.06 QDD182 pKa = 4.01 TDD184 pKa = 3.17 IGDD187 pKa = 4.55 IIDD190 pKa = 4.64 KK191 pKa = 9.77 CKK193 pKa = 10.43 QWIAYY198 pKa = 8.08 QPYY201 pKa = 7.75 VQMMATIDD209 pKa = 3.44 MFLNEE214 pKa = 4.98 FPFHH218 pKa = 7.29 PYY220 pKa = 8.59 SQARR224 pKa = 11.84 IGTIVTRR231 pKa = 11.84 FKK233 pKa = 11.24 DD234 pKa = 3.84 CAALIATRR242 pKa = 11.84 MITQCLGLKK251 pKa = 10.12 FPEE254 pKa = 3.98 FAEE257 pKa = 4.74 WIWTSRR263 pKa = 11.84 CADD266 pKa = 3.23 QFEE269 pKa = 4.76 RR270 pKa = 11.84 IIKK273 pKa = 10.2 GDD275 pKa = 3.55 EE276 pKa = 4.06 EE277 pKa = 3.97 MDD279 pKa = 3.59 EE280 pKa = 4.13 PRR282 pKa = 11.84 SYY284 pKa = 11.99 SMYY287 pKa = 10.79 FMDD290 pKa = 5.99 LGLSSKK296 pKa = 10.5 SPYY299 pKa = 9.2 SASVNLDD306 pKa = 2.79 LHH308 pKa = 6.51 YY309 pKa = 10.58 FFHH312 pKa = 6.68 TLGVSAGLEE321 pKa = 3.74 RR322 pKa = 11.84 SKK324 pKa = 10.68 RR325 pKa = 11.84 ARR327 pKa = 11.84 VVGDD331 pKa = 3.51 PEE333 pKa = 4.28 VNNIISNAVVMHH345 pKa = 5.47 YY346 pKa = 11.37 VMGRR350 pKa = 11.84 FATLSQQFGVDD361 pKa = 3.57 GEE363 pKa = 4.36 GDD365 pKa = 3.56 EE366 pKa = 6.52 LEE368 pKa = 4.99 DD369 pKa = 3.68 EE370 pKa = 4.95 NNRR373 pKa = 11.84 PMPEE377 pKa = 4.24 GEE379 pKa = 4.12 PQEE382 pKa = 4.54 KK383 pKa = 10.47 DD384 pKa = 3.09 SSLWLAYY391 pKa = 9.73 IMRR394 pKa = 11.84 NNGKK398 pKa = 9.25 VPDD401 pKa = 4.08 YY402 pKa = 10.51 IVRR405 pKa = 11.84 KK406 pKa = 9.65 VATEE410 pKa = 4.23 WEE412 pKa = 4.26 QNPACRR418 pKa = 11.84 DD419 pKa = 3.55 GTIGKK424 pKa = 9.39 ALYY427 pKa = 10.14 DD428 pKa = 3.36 KK429 pKa = 11.11 SGPLLLNN436 pKa = 4.1
Molecular weight: 49.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.009
IPC2_protein 5.054
IPC_protein 5.016
Toseland 4.94
ProMoST 5.156
Dawson 5.003
Bjellqvist 5.13
Wikipedia 4.889
Rodwell 4.914
Grimsley 4.851
Solomon 5.003
Lehninger 4.965
Nozaki 5.118
DTASelect 5.296
Thurlkill 4.94
EMBOSS 4.927
Sillero 5.194
Patrickios 4.012
IPC_peptide 5.016
IPC2_peptide 5.181
IPC2.peptide.svr19 5.188
Protein with the highest isoelectric point:
>tr|A0A140D058|A0A140D058_9RHAB Matrix protein OS=Riverside virus 1 OX=1803263 GN=M PE=4 SV=1
MM1 pKa = 7.54 SIIKK5 pKa = 9.9 IVRR8 pKa = 11.84 VDD10 pKa = 3.5 ALLEE14 pKa = 3.86 MRR16 pKa = 11.84 FRR18 pKa = 11.84 EE19 pKa = 4.22 PPRR22 pKa = 11.84 TYY24 pKa = 11.07 KK25 pKa = 10.74 DD26 pKa = 3.47 LLSSLEE32 pKa = 4.24 IIKK35 pKa = 10.43 DD36 pKa = 3.57 DD37 pKa = 3.56 YY38 pKa = 11.23 KK39 pKa = 11.52 GYY41 pKa = 11.24 VNDD44 pKa = 4.41 KK45 pKa = 10.59 EE46 pKa = 4.47 YY47 pKa = 10.8 ILLFYY52 pKa = 9.94 TLAGFTCKK60 pKa = 9.9 YY61 pKa = 8.1 RR62 pKa = 11.84 HH63 pKa = 5.2 QVGAFYY69 pKa = 10.26 HH70 pKa = 5.42 YY71 pKa = 10.13 RR72 pKa = 11.84 GTINGVFKK80 pKa = 10.75 IKK82 pKa = 9.11 MCSMSPIFPLTYY94 pKa = 10.09 DD95 pKa = 3.61 YY96 pKa = 11.4 DD97 pKa = 4.28 GEE99 pKa = 4.86 GIGLIPNLLVSFHH112 pKa = 6.12 LTSEE116 pKa = 4.28 EE117 pKa = 4.7 SNMSPTSIVDD127 pKa = 3.72 YY128 pKa = 10.64 IRR130 pKa = 11.84 SRR132 pKa = 11.84 SPNKK136 pKa = 9.09 YY137 pKa = 9.55 RR138 pKa = 11.84 KK139 pKa = 9.02 RR140 pKa = 11.84 HH141 pKa = 4.89 ALKK144 pKa = 10.74 LSIFKK149 pKa = 10.19 PYY151 pKa = 10.46 AITVEE156 pKa = 4.19 SEE158 pKa = 3.95 KK159 pKa = 11.18 DD160 pKa = 3.3 CLSIKK165 pKa = 10.65 KK166 pKa = 9.84 NN167 pKa = 3.41
Molecular weight: 19.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.667
IPC2_protein 8.668
IPC_protein 8.609
Toseland 9.048
ProMoST 8.975
Dawson 9.399
Bjellqvist 9.238
Wikipedia 9.604
Rodwell 9.531
Grimsley 9.472
Solomon 9.472
Lehninger 9.414
Nozaki 9.238
DTASelect 9.165
Thurlkill 9.238
EMBOSS 9.502
Sillero 9.385
Patrickios 4.24
IPC_peptide 9.458
IPC2_peptide 7.951
IPC2.peptide.svr19 7.737
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3656
167
2122
731.2
82.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.787 ± 0.74
1.422 ± 0.278
5.771 ± 0.356
5.388 ± 0.714
4.212 ± 0.173
6.072 ± 0.459
2.571 ± 0.436
6.838 ± 0.544
6.154 ± 0.637
9.874 ± 0.863
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.489 ± 0.34
4.814 ± 0.353
5.17 ± 0.62
3.556 ± 0.44
5.142 ± 0.467
8.124 ± 0.755
6.209 ± 0.679
5.826 ± 0.364
1.723 ± 0.393
3.857 ± 0.673
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here