Rhodobacter phage RC1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4SNW4|M4SNW4_9CAUD Uncharacterized protein OS=Rhodobacter phage RC1 OX=754055 GN=RHWG_00046 PE=4 SV=1
MM1 pKa = 7.8SDD3 pKa = 3.69IDD5 pKa = 3.91QEE7 pKa = 4.12DD8 pKa = 4.63AIAIGTTIVEE18 pKa = 3.97MADD21 pKa = 3.26RR22 pKa = 11.84VKK24 pKa = 10.41TMDD27 pKa = 4.24LVCPGTAAEE36 pKa = 4.63WNFEE40 pKa = 4.17LDD42 pKa = 3.65DD43 pKa = 3.47QRR45 pKa = 11.84FDD47 pKa = 3.54VKK49 pKa = 10.45VTVAVPKK56 pKa = 10.69NSIPEE61 pKa = 4.11EE62 pKa = 4.16EE63 pKa = 4.28TT64 pKa = 3.27

Molecular weight: 7.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4STB6|M4STB6_9CAUD Uncharacterized protein OS=Rhodobacter phage RC1 OX=754055 GN=RHWG_00040 PE=4 SV=1
MM1 pKa = 7.17KK2 pKa = 10.29AIYY5 pKa = 10.49ALARR9 pKa = 11.84DD10 pKa = 4.16LQIDD14 pKa = 3.68DD15 pKa = 4.14DD16 pKa = 4.46DD17 pKa = 6.1RR18 pKa = 11.84RR19 pKa = 11.84DD20 pKa = 3.15LYY22 pKa = 11.32QRR24 pKa = 11.84VTGKK28 pKa = 10.56RR29 pKa = 11.84SLSDD33 pKa = 3.1MTAEE37 pKa = 3.97QQKK40 pKa = 9.71QVASEE45 pKa = 3.83LRR47 pKa = 11.84RR48 pKa = 11.84LLDD51 pKa = 3.44RR52 pKa = 11.84PGVGRR57 pKa = 11.84IQLTGPYY64 pKa = 9.68AKK66 pKa = 10.26KK67 pKa = 10.39LQALWIAGWNLGLVRR82 pKa = 11.84NRR84 pKa = 11.84EE85 pKa = 4.02DD86 pKa = 3.06AALIAFVKK94 pKa = 10.02RR95 pKa = 11.84QTGIDD100 pKa = 3.29HH101 pKa = 6.52VRR103 pKa = 11.84FLRR106 pKa = 11.84DD107 pKa = 3.07PFQATKK113 pKa = 10.52AVEE116 pKa = 3.93ALKK119 pKa = 10.77DD120 pKa = 3.12WLRR123 pKa = 11.84RR124 pKa = 11.84DD125 pKa = 3.61GGVVWDD131 pKa = 3.89TQGYY135 pKa = 10.12DD136 pKa = 2.52WFADD140 pKa = 3.58HH141 pKa = 6.77GAKK144 pKa = 9.8IAWAQWRR151 pKa = 11.84LLHH154 pKa = 7.06PDD156 pKa = 3.05ATLLQIGGFKK166 pKa = 10.87AEE168 pKa = 4.28AGRR171 pKa = 11.84LAGRR175 pKa = 11.84AARR178 pKa = 11.84QVPLSEE184 pKa = 4.21FTSADD189 pKa = 2.39WRR191 pKa = 11.84AICNALGHH199 pKa = 5.36RR200 pKa = 11.84VRR202 pKa = 11.84RR203 pKa = 11.84ISGG206 pKa = 3.18

Molecular weight: 23.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12437

59

1231

222.1

24.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.085 ± 0.566

0.756 ± 0.135

6.256 ± 0.311

6.802 ± 0.319

2.903 ± 0.205

7.936 ± 0.286

1.737 ± 0.198

5.226 ± 0.238

4.109 ± 0.304

9.142 ± 0.306

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.613 ± 0.246

2.613 ± 0.251

4.865 ± 0.33

3.859 ± 0.401

7.381 ± 0.408

6.207 ± 0.354

5.733 ± 0.381

6.191 ± 0.421

1.769 ± 0.161

1.817 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski