Amino acid dipepetide frequency for Rhodobacter phage RC1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.082AlaAla: 16.082 ± 2.434
0.804AlaCys: 0.804 ± 0.23
6.433AlaAsp: 6.433 ± 0.676
7.559AlaGlu: 7.559 ± 0.734
3.86AlaPhe: 3.86 ± 0.714
9.087AlaGly: 9.087 ± 1.029
1.045AlaHis: 1.045 ± 0.259
5.95AlaIle: 5.95 ± 0.623
5.87AlaLys: 5.87 ± 0.914
11.821AlaLeu: 11.821 ± 0.979
2.814AlaMet: 2.814 ± 0.613
3.297AlaAsn: 3.297 ± 0.85
5.709AlaPro: 5.709 ± 0.596
4.744AlaGln: 4.744 ± 1.07
9.247AlaArg: 9.247 ± 0.785
7.8AlaSer: 7.8 ± 1.066
7.398AlaThr: 7.398 ± 0.843
7.639AlaVal: 7.639 ± 0.777
2.412AlaTrp: 2.412 ± 0.385
1.206AlaTyr: 1.206 ± 0.332
0.0AlaXaa: 0.0 ± 0.0
Cys
0.804CysAla: 0.804 ± 0.29
0.322CysCys: 0.322 ± 0.14
0.241CysAsp: 0.241 ± 0.151
0.563CysGlu: 0.563 ± 0.216
0.161CysPhe: 0.161 ± 0.092
0.563CysGly: 0.563 ± 0.217
0.161CysHis: 0.161 ± 0.108
0.322CysIle: 0.322 ± 0.145
0.161CysLys: 0.161 ± 0.112
0.482CysLeu: 0.482 ± 0.204
0.241CysMet: 0.241 ± 0.112
0.241CysAsn: 0.241 ± 0.156
0.885CysPro: 0.885 ± 0.304
0.161CysGln: 0.161 ± 0.111
0.563CysArg: 0.563 ± 0.265
0.724CysSer: 0.724 ± 0.27
0.322CysThr: 0.322 ± 0.129
0.241CysVal: 0.241 ± 0.134
0.161CysTrp: 0.161 ± 0.111
0.241CysTyr: 0.241 ± 0.143
0.0CysXaa: 0.0 ± 0.0
Asp
8.926AspAla: 8.926 ± 0.815
0.402AspCys: 0.402 ± 0.216
3.538AspAsp: 3.538 ± 0.638
4.342AspGlu: 4.342 ± 0.58
2.252AspPhe: 2.252 ± 0.377
5.709AspGly: 5.709 ± 0.773
1.608AspHis: 1.608 ± 0.386
3.216AspIle: 3.216 ± 0.463
1.689AspLys: 1.689 ± 0.414
7.157AspLeu: 7.157 ± 0.723
1.528AspMet: 1.528 ± 0.276
1.528AspAsn: 1.528 ± 0.34
4.181AspPro: 4.181 ± 0.628
3.136AspGln: 3.136 ± 0.468
3.619AspArg: 3.619 ± 0.5
1.689AspSer: 1.689 ± 0.34
2.412AspThr: 2.412 ± 0.527
2.493AspVal: 2.493 ± 0.421
1.528AspTrp: 1.528 ± 0.401
1.608AspTyr: 1.608 ± 0.395
0.0AspXaa: 0.0 ± 0.0
Glu
8.684GluAla: 8.684 ± 0.843
0.563GluCys: 0.563 ± 0.224
3.297GluAsp: 3.297 ± 0.608
4.021GluGlu: 4.021 ± 0.582
2.654GluPhe: 2.654 ± 0.581
3.377GluGly: 3.377 ± 0.483
1.528GluHis: 1.528 ± 0.305
5.548GluIle: 5.548 ± 0.714
3.056GluLys: 3.056 ± 0.484
7.478GluLeu: 7.478 ± 0.637
2.091GluMet: 2.091 ± 0.391
2.412GluAsn: 2.412 ± 0.444
2.412GluPro: 2.412 ± 0.543
3.458GluGln: 3.458 ± 0.668
4.583GluArg: 4.583 ± 0.936
2.252GluSer: 2.252 ± 0.369
4.503GluThr: 4.503 ± 0.54
3.94GluVal: 3.94 ± 0.527
1.045GluTrp: 1.045 ± 0.361
1.126GluTyr: 1.126 ± 0.262
0.0GluXaa: 0.0 ± 0.0
Phe
3.216PheAla: 3.216 ± 0.426
0.402PheCys: 0.402 ± 0.167
2.654PheAsp: 2.654 ± 0.507
2.171PheGlu: 2.171 ± 0.519
0.482PhePhe: 0.482 ± 0.208
3.216PheGly: 3.216 ± 0.466
0.322PheHis: 0.322 ± 0.16
0.724PheIle: 0.724 ± 0.234
1.608PheLys: 1.608 ± 0.418
1.447PheLeu: 1.447 ± 0.299
1.206PheMet: 1.206 ± 0.32
1.045PheAsn: 1.045 ± 0.225
0.724PhePro: 0.724 ± 0.315
1.206PheGln: 1.206 ± 0.333
2.01PheArg: 2.01 ± 0.367
2.493PheSer: 2.493 ± 0.467
1.689PheThr: 1.689 ± 0.567
1.849PheVal: 1.849 ± 0.348
0.241PheTrp: 0.241 ± 0.153
0.322PheTyr: 0.322 ± 0.197
0.0PheXaa: 0.0 ± 0.0
Gly
10.695GlyAla: 10.695 ± 0.862
0.482GlyCys: 0.482 ± 0.233
4.423GlyAsp: 4.423 ± 0.585
4.101GlyGlu: 4.101 ± 0.556
2.412GlyPhe: 2.412 ± 0.424
5.79GlyGly: 5.79 ± 0.892
2.01GlyHis: 2.01 ± 0.426
4.181GlyIle: 4.181 ± 0.763
3.056GlyLys: 3.056 ± 0.36
7.8GlyLeu: 7.8 ± 0.9
2.171GlyMet: 2.171 ± 0.41
2.091GlyAsn: 2.091 ± 0.408
3.136GlyPro: 3.136 ± 0.44
2.895GlyGln: 2.895 ± 0.582
5.87GlyArg: 5.87 ± 0.767
4.986GlySer: 4.986 ± 0.737
4.986GlyThr: 4.986 ± 0.635
5.388GlyVal: 5.388 ± 0.773
1.447GlyTrp: 1.447 ± 0.307
1.447GlyTyr: 1.447 ± 0.358
0.0GlyXaa: 0.0 ± 0.0
His
1.287HisAla: 1.287 ± 0.318
0.161HisCys: 0.161 ± 0.106
1.367HisAsp: 1.367 ± 0.34
0.965HisGlu: 0.965 ± 0.212
0.804HisPhe: 0.804 ± 0.235
1.689HisGly: 1.689 ± 0.345
0.402HisHis: 0.402 ± 0.166
0.643HisIle: 0.643 ± 0.252
0.161HisLys: 0.161 ± 0.095
1.849HisLeu: 1.849 ± 0.383
0.643HisMet: 0.643 ± 0.286
0.563HisAsn: 0.563 ± 0.24
0.724HisPro: 0.724 ± 0.268
0.643HisGln: 0.643 ± 0.197
1.689HisArg: 1.689 ± 0.385
1.287HisSer: 1.287 ± 0.36
0.482HisThr: 0.482 ± 0.196
1.126HisVal: 1.126 ± 0.248
0.322HisTrp: 0.322 ± 0.174
0.563HisTyr: 0.563 ± 0.172
0.0HisXaa: 0.0 ± 0.0
Ile
6.111IleAla: 6.111 ± 0.618
0.643IleCys: 0.643 ± 0.213
5.146IleAsp: 5.146 ± 0.618
5.468IleGlu: 5.468 ± 0.62
0.965IlePhe: 0.965 ± 0.327
4.583IleGly: 4.583 ± 0.588
0.643IleHis: 0.643 ± 0.239
1.689IleIle: 1.689 ± 0.32
1.528IleLys: 1.528 ± 0.348
3.86IleLeu: 3.86 ± 0.467
0.322IleMet: 0.322 ± 0.157
1.126IleAsn: 1.126 ± 0.293
2.654IlePro: 2.654 ± 0.374
1.447IleGln: 1.447 ± 0.268
3.538IleArg: 3.538 ± 0.449
3.779IleSer: 3.779 ± 0.429
2.975IleThr: 2.975 ± 0.754
3.297IleVal: 3.297 ± 0.652
1.045IleTrp: 1.045 ± 0.181
1.447IleTyr: 1.447 ± 0.357
0.0IleXaa: 0.0 ± 0.0
Lys
5.629LysAla: 5.629 ± 0.685
0.322LysCys: 0.322 ± 0.17
1.367LysAsp: 1.367 ± 0.29
2.573LysGlu: 2.573 ± 0.439
0.965LysPhe: 0.965 ± 0.231
3.86LysGly: 3.86 ± 0.545
0.804LysHis: 0.804 ± 0.278
2.493LysIle: 2.493 ± 0.584
1.126LysLys: 1.126 ± 0.242
4.101LysLeu: 4.101 ± 0.547
0.643LysMet: 0.643 ± 0.216
0.563LysAsn: 0.563 ± 0.199
2.493LysPro: 2.493 ± 0.639
1.447LysGln: 1.447 ± 0.345
3.297LysArg: 3.297 ± 0.533
2.412LysSer: 2.412 ± 0.367
3.297LysThr: 3.297 ± 0.701
2.332LysVal: 2.332 ± 0.544
0.804LysTrp: 0.804 ± 0.267
0.563LysTyr: 0.563 ± 0.164
0.0LysXaa: 0.0 ± 0.0
Leu
10.373LeuAla: 10.373 ± 0.869
0.804LeuCys: 0.804 ± 0.32
6.192LeuAsp: 6.192 ± 0.843
6.111LeuGlu: 6.111 ± 0.699
2.171LeuPhe: 2.171 ± 0.407
7.559LeuGly: 7.559 ± 1.062
1.367LeuHis: 1.367 ± 0.303
4.664LeuIle: 4.664 ± 0.738
3.699LeuLys: 3.699 ± 0.562
7.076LeuLeu: 7.076 ± 0.707
2.493LeuMet: 2.493 ± 0.436
2.814LeuAsn: 2.814 ± 0.473
4.744LeuPro: 4.744 ± 0.554
3.377LeuGln: 3.377 ± 0.608
7.076LeuArg: 7.076 ± 0.539
6.594LeuSer: 6.594 ± 0.744
5.79LeuThr: 5.79 ± 0.657
5.468LeuVal: 5.468 ± 0.595
1.367LeuTrp: 1.367 ± 0.374
1.689LeuTyr: 1.689 ± 0.344
0.0LeuXaa: 0.0 ± 0.0
Met
2.895MetAla: 2.895 ± 0.468
0.08MetCys: 0.08 ± 0.076
1.126MetAsp: 1.126 ± 0.308
1.608MetGlu: 1.608 ± 0.365
0.563MetPhe: 0.563 ± 0.223
1.849MetGly: 1.849 ± 0.371
0.724MetHis: 0.724 ± 0.216
1.206MetIle: 1.206 ± 0.292
1.287MetLys: 1.287 ± 0.302
2.412MetLeu: 2.412 ± 0.496
0.804MetMet: 0.804 ± 0.238
0.482MetAsn: 0.482 ± 0.171
1.608MetPro: 1.608 ± 0.396
1.287MetGln: 1.287 ± 0.293
2.332MetArg: 2.332 ± 0.441
2.171MetSer: 2.171 ± 0.396
2.091MetThr: 2.091 ± 0.417
0.724MetVal: 0.724 ± 0.243
0.563MetTrp: 0.563 ± 0.266
0.322MetTyr: 0.322 ± 0.131
0.0MetXaa: 0.0 ± 0.0
Asn
3.297AsnAla: 3.297 ± 0.799
0.161AsnCys: 0.161 ± 0.16
2.252AsnAsp: 2.252 ± 0.433
0.965AsnGlu: 0.965 ± 0.237
0.724AsnPhe: 0.724 ± 0.215
2.412AsnGly: 2.412 ± 0.455
0.402AsnHis: 0.402 ± 0.155
1.367AsnIle: 1.367 ± 0.302
0.885AsnLys: 0.885 ± 0.258
2.412AsnLeu: 2.412 ± 0.577
0.402AsnMet: 0.402 ± 0.179
0.643AsnAsn: 0.643 ± 0.246
1.849AsnPro: 1.849 ± 0.429
0.643AsnGln: 0.643 ± 0.372
1.528AsnArg: 1.528 ± 0.359
2.01AsnSer: 2.01 ± 0.323
1.689AsnThr: 1.689 ± 0.378
1.528AsnVal: 1.528 ± 0.325
0.563AsnTrp: 0.563 ± 0.167
0.402AsnTyr: 0.402 ± 0.167
0.0AsnXaa: 0.0 ± 0.0
Pro
5.388ProAla: 5.388 ± 0.737
0.08ProCys: 0.08 ± 0.076
3.136ProAsp: 3.136 ± 0.518
3.216ProGlu: 3.216 ± 0.539
1.608ProPhe: 1.608 ± 0.323
4.101ProGly: 4.101 ± 0.537
1.126ProHis: 1.126 ± 0.348
1.849ProIle: 1.849 ± 0.37
2.332ProLys: 2.332 ± 0.522
4.262ProLeu: 4.262 ± 0.543
1.367ProMet: 1.367 ± 0.313
0.885ProAsn: 0.885 ± 0.302
2.091ProPro: 2.091 ± 0.348
1.689ProGln: 1.689 ± 0.331
4.021ProArg: 4.021 ± 0.644
3.538ProSer: 3.538 ± 0.617
1.93ProThr: 1.93 ± 0.371
3.779ProVal: 3.779 ± 0.667
1.206ProTrp: 1.206 ± 0.476
1.045ProTyr: 1.045 ± 0.252
0.0ProXaa: 0.0 ± 0.0
Gln
5.146GlnAla: 5.146 ± 0.758
0.08GlnCys: 0.08 ± 0.078
1.287GlnAsp: 1.287 ± 0.241
2.493GlnGlu: 2.493 ± 0.473
1.045GlnPhe: 1.045 ± 0.222
2.332GlnGly: 2.332 ± 0.522
0.724GlnHis: 0.724 ± 0.212
2.975GlnIle: 2.975 ± 0.534
1.849GlnLys: 1.849 ± 0.392
4.101GlnLeu: 4.101 ± 0.552
1.367GlnMet: 1.367 ± 0.333
0.885GlnAsn: 0.885 ± 0.261
1.93GlnPro: 1.93 ± 0.497
1.769GlnGln: 1.769 ± 0.668
2.412GlnArg: 2.412 ± 0.382
1.447GlnSer: 1.447 ± 0.444
2.895GlnThr: 2.895 ± 0.61
2.493GlnVal: 2.493 ± 0.369
0.563GlnTrp: 0.563 ± 0.221
0.804GlnTyr: 0.804 ± 0.295
0.0GlnXaa: 0.0 ± 0.0
Arg
7.559ArgAla: 7.559 ± 0.788
0.482ArgCys: 0.482 ± 0.268
5.307ArgAsp: 5.307 ± 0.698
5.146ArgGlu: 5.146 ± 0.688
2.734ArgPhe: 2.734 ± 0.447
4.825ArgGly: 4.825 ± 0.649
1.608ArgHis: 1.608 ± 0.361
3.136ArgIle: 3.136 ± 0.458
4.342ArgLys: 4.342 ± 0.826
6.915ArgLeu: 6.915 ± 0.787
1.689ArgMet: 1.689 ± 0.53
2.01ArgAsn: 2.01 ± 0.435
3.458ArgPro: 3.458 ± 0.604
2.573ArgGln: 2.573 ± 0.485
6.433ArgArg: 6.433 ± 0.962
4.744ArgSer: 4.744 ± 0.454
2.332ArgThr: 2.332 ± 0.428
5.629ArgVal: 5.629 ± 0.821
1.206ArgTrp: 1.206 ± 0.196
1.689ArgTyr: 1.689 ± 0.392
0.0ArgXaa: 0.0 ± 0.0
Ser
6.835SerAla: 6.835 ± 1.213
0.563SerCys: 0.563 ± 0.249
5.146SerAsp: 5.146 ± 0.5
5.307SerGlu: 5.307 ± 0.564
2.171SerPhe: 2.171 ± 0.453
6.594SerGly: 6.594 ± 0.943
0.643SerHis: 0.643 ± 0.207
3.86SerIle: 3.86 ± 0.608
2.332SerLys: 2.332 ± 0.418
4.423SerLeu: 4.423 ± 0.605
1.528SerMet: 1.528 ± 0.339
1.849SerAsn: 1.849 ± 0.422
2.493SerPro: 2.493 ± 0.484
2.091SerGln: 2.091 ± 0.435
4.342SerArg: 4.342 ± 0.561
4.423SerSer: 4.423 ± 0.628
3.458SerThr: 3.458 ± 0.502
2.493SerVal: 2.493 ± 0.406
0.563SerTrp: 0.563 ± 0.229
0.965SerTyr: 0.965 ± 0.322
0.0SerXaa: 0.0 ± 0.0
Thr
5.95ThrAla: 5.95 ± 0.863
0.482ThrCys: 0.482 ± 0.226
4.664ThrAsp: 4.664 ± 0.785
4.583ThrGlu: 4.583 ± 0.607
1.447ThrPhe: 1.447 ± 0.383
4.825ThrGly: 4.825 ± 0.564
1.206ThrHis: 1.206 ± 0.273
3.216ThrIle: 3.216 ± 0.544
2.654ThrLys: 2.654 ± 0.535
4.664ThrLeu: 4.664 ± 0.559
1.689ThrMet: 1.689 ± 0.344
1.206ThrAsn: 1.206 ± 0.314
2.573ThrPro: 2.573 ± 0.45
2.171ThrGln: 2.171 ± 0.355
2.654ThrArg: 2.654 ± 0.427
3.056ThrSer: 3.056 ± 0.496
3.699ThrThr: 3.699 ± 0.585
4.423ThrVal: 4.423 ± 0.77
1.126ThrTrp: 1.126 ± 0.31
1.045ThrTyr: 1.045 ± 0.298
0.0ThrXaa: 0.0 ± 0.0
Val
7.961ValAla: 7.961 ± 0.618
0.402ValCys: 0.402 ± 0.157
2.895ValAsp: 2.895 ± 0.576
5.227ValGlu: 5.227 ± 0.626
1.608ValPhe: 1.608 ± 0.416
3.216ValGly: 3.216 ± 0.734
0.643ValHis: 0.643 ± 0.2
3.377ValIle: 3.377 ± 0.578
2.734ValLys: 2.734 ± 0.43
5.227ValLeu: 5.227 ± 0.651
1.769ValMet: 1.769 ± 0.439
1.126ValAsn: 1.126 ± 0.316
3.136ValPro: 3.136 ± 0.487
2.412ValGln: 2.412 ± 0.57
4.986ValArg: 4.986 ± 0.677
4.423ValSer: 4.423 ± 0.679
3.619ValThr: 3.619 ± 0.559
4.744ValVal: 4.744 ± 0.895
1.206ValTrp: 1.206 ± 0.301
1.206ValTyr: 1.206 ± 0.356
0.0ValXaa: 0.0 ± 0.0
Trp
1.608TrpAla: 1.608 ± 0.389
0.08TrpCys: 0.08 ± 0.071
1.045TrpAsp: 1.045 ± 0.369
0.804TrpGlu: 0.804 ± 0.225
0.241TrpPhe: 0.241 ± 0.14
1.849TrpGly: 1.849 ± 0.416
0.402TrpHis: 0.402 ± 0.195
1.045TrpIle: 1.045 ± 0.289
0.563TrpLys: 0.563 ± 0.222
1.367TrpLeu: 1.367 ± 0.254
0.724TrpMet: 0.724 ± 0.26
0.965TrpAsn: 0.965 ± 0.254
1.126TrpPro: 1.126 ± 0.348
0.563TrpGln: 0.563 ± 0.198
1.689TrpArg: 1.689 ± 0.327
1.045TrpSer: 1.045 ± 0.254
1.126TrpThr: 1.126 ± 0.297
1.126TrpVal: 1.126 ± 0.331
0.161TrpTrp: 0.161 ± 0.119
0.161TrpTyr: 0.161 ± 0.119
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.412TyrAla: 2.412 ± 0.435
0.241TyrCys: 0.241 ± 0.137
1.045TyrAsp: 1.045 ± 0.319
0.804TyrGlu: 0.804 ± 0.229
0.161TyrPhe: 0.161 ± 0.094
1.93TyrGly: 1.93 ± 0.467
0.0TyrHis: 0.0 ± 0.0
0.804TyrIle: 0.804 ± 0.268
0.161TyrLys: 0.161 ± 0.093
2.573TyrLeu: 2.573 ± 0.397
0.563TyrMet: 0.563 ± 0.245
0.402TyrAsn: 0.402 ± 0.16
0.724TyrPro: 0.724 ± 0.323
0.885TyrGln: 0.885 ± 0.245
1.93TyrArg: 1.93 ± 0.482
1.206TyrSer: 1.206 ± 0.315
0.643TyrThr: 0.643 ± 0.237
1.206TyrVal: 1.206 ± 0.33
0.161TyrTrp: 0.161 ± 0.107
0.322TyrTyr: 0.322 ± 0.134
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (12437 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski