Rhesus adenovirus 58
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H5AI91|A0A2H5AI91_9ADEN Penton protein OS=Rhesus adenovirus 58 OX=2064734 GN=L2 PE=2 SV=1
MM1 pKa = 7.61 RR2 pKa = 11.84 LVTEE6 pKa = 4.43 MFDD9 pKa = 3.41 VFYY12 pKa = 11.38 DD13 pKa = 3.94 EE14 pKa = 4.5 MARR17 pKa = 11.84 NSDD20 pKa = 3.64 EE21 pKa = 4.69 LLNSDD26 pKa = 4.82 LLDD29 pKa = 3.78 VPGTPVLSPPSLHH42 pKa = 7.34 DD43 pKa = 4.07 LFDD46 pKa = 4.15 VEE48 pKa = 4.66 VDD50 pKa = 3.58 PPEE53 pKa = 5.04 DD54 pKa = 3.83 PNEE57 pKa = 4.31 DD58 pKa = 3.2 AVNTLFPDD66 pKa = 4.25 FMLEE70 pKa = 3.7 AAEE73 pKa = 4.56 EE74 pKa = 4.96 GSHH77 pKa = 6.7 SGGEE81 pKa = 4.19 SGRR84 pKa = 11.84 SGDD87 pKa = 4.4 EE88 pKa = 4.22 LDD90 pKa = 3.64 LKK92 pKa = 10.89 CYY94 pKa = 9.61 EE95 pKa = 4.13 EE96 pKa = 4.86 CLPSSEE102 pKa = 4.76 SEE104 pKa = 3.86 SDD106 pKa = 3.53 EE107 pKa = 4.79 ADD109 pKa = 3.08 GCAEE113 pKa = 3.72 QTVKK117 pKa = 11.15 NEE119 pKa = 4.74 LILDD123 pKa = 4.19 CPEE126 pKa = 4.72 HH127 pKa = 6.86 PGHH130 pKa = 7.46 DD131 pKa = 3.36 CRR133 pKa = 11.84 ACDD136 pKa = 3.75 FHH138 pKa = 8.9 RR139 pKa = 11.84 SASGNPEE146 pKa = 3.99 TLCALCYY153 pKa = 10.42 LRR155 pKa = 11.84 LTSDD159 pKa = 3.94 FVYY162 pKa = 11.02 SKK164 pKa = 11.51
Molecular weight: 18.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.897
IPC_protein 3.872
Toseland 3.681
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.757
Rodwell 3.706
Grimsley 3.579
Solomon 3.846
Lehninger 3.795
Nozaki 3.961
DTASelect 4.151
Thurlkill 3.719
EMBOSS 3.77
Sillero 3.986
Patrickios 0.871
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.881
Protein with the highest isoelectric point:
>tr|A0A2H5AIA4|A0A2H5AIA4_9ADEN Isoform of A0A2H5AI85 Protease OS=Rhesus adenovirus 58 OX=2064734 GN=L3 PE=2 SV=1
MM1 pKa = 7.68 SKK3 pKa = 10.58 RR4 pKa = 11.84 KK5 pKa = 9.36 FKK7 pKa = 11.23 EE8 pKa = 3.69 EE9 pKa = 3.77 LLQTLVPEE17 pKa = 4.89 IYY19 pKa = 10.45 GPPDD23 pKa = 3.68 EE24 pKa = 5.68 KK25 pKa = 11.04 PDD27 pKa = 3.38 IKK29 pKa = 10.81 PRR31 pKa = 11.84 DD32 pKa = 3.37 IKK34 pKa = 10.63 RR35 pKa = 11.84 VKK37 pKa = 10.18 KK38 pKa = 10.27 RR39 pKa = 11.84 EE40 pKa = 4.09 KK41 pKa = 10.28 KK42 pKa = 10.22 EE43 pKa = 3.62 EE44 pKa = 3.97 LAVVDD49 pKa = 4.07 DD50 pKa = 4.53 GGVEE54 pKa = 4.65 FVRR57 pKa = 11.84 SFAPRR62 pKa = 11.84 RR63 pKa = 11.84 RR64 pKa = 11.84 VQWKK68 pKa = 7.65 GRR70 pKa = 11.84 RR71 pKa = 11.84 VKK73 pKa = 10.47 RR74 pKa = 11.84 VLRR77 pKa = 11.84 PGTAVVFTPGEE88 pKa = 3.9 RR89 pKa = 11.84 SAVRR93 pKa = 11.84 GFKK96 pKa = 10.07 RR97 pKa = 11.84 QYY99 pKa = 10.65 DD100 pKa = 3.87 EE101 pKa = 5.1 VYY103 pKa = 10.34 GDD105 pKa = 3.95 EE106 pKa = 6.16 DD107 pKa = 3.53 ILEE110 pKa = 4.12 QAAQQIGEE118 pKa = 4.01 FAYY121 pKa = 10.19 GKK123 pKa = 10.22 RR124 pKa = 11.84 SRR126 pKa = 11.84 QEE128 pKa = 3.74 DD129 pKa = 3.67 LAIALDD135 pKa = 4.67 DD136 pKa = 4.65 GNPTPSLKK144 pKa = 10.31 PVTLQQVLPVSASADD159 pKa = 3.29 SKK161 pKa = 11.03 RR162 pKa = 11.84 GIKK165 pKa = 9.99 RR166 pKa = 11.84 EE167 pKa = 3.86 MDD169 pKa = 3.68 DD170 pKa = 4.16 LQPTIQLMVPKK181 pKa = 9.88 RR182 pKa = 11.84 QRR184 pKa = 11.84 LEE186 pKa = 3.86 EE187 pKa = 4.07 VLEE190 pKa = 4.11 KK191 pKa = 10.62 MKK193 pKa = 10.44 VDD195 pKa = 3.85 PSIQPEE201 pKa = 4.47 VKK203 pKa = 9.64 VRR205 pKa = 11.84 PIKK208 pKa = 10.31 EE209 pKa = 3.96 VAPGLGVQTVDD220 pKa = 2.53 IQIPVRR226 pKa = 11.84 SASTAVEE233 pKa = 3.84 AMEE236 pKa = 4.55 TQTEE240 pKa = 4.39 TPVAVGTRR248 pKa = 11.84 EE249 pKa = 3.92 VALQTDD255 pKa = 3.78 PWYY258 pKa = 10.37 EE259 pKa = 3.65 YY260 pKa = 10.81 AAPRR264 pKa = 11.84 RR265 pKa = 11.84 QRR267 pKa = 11.84 RR268 pKa = 11.84 TGRR271 pKa = 11.84 YY272 pKa = 8.34 GPASAIMPEE281 pKa = 4.46 YY282 pKa = 10.32 ALHH285 pKa = 6.99 PSIQPTPGYY294 pKa = 10.0 RR295 pKa = 11.84 GVTYY299 pKa = 10.27 RR300 pKa = 11.84 PSATRR305 pKa = 11.84 RR306 pKa = 11.84 RR307 pKa = 11.84 SRR309 pKa = 11.84 RR310 pKa = 11.84 RR311 pKa = 11.84 RR312 pKa = 11.84 RR313 pKa = 11.84 SRR315 pKa = 11.84 RR316 pKa = 11.84 PLAPVSVRR324 pKa = 11.84 RR325 pKa = 11.84 VTRR328 pKa = 11.84 RR329 pKa = 11.84 GKK331 pKa = 8.55 TVTIPNPRR339 pKa = 11.84 YY340 pKa = 9.63 HH341 pKa = 7.15 PSILL345 pKa = 3.57
Molecular weight: 39.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.298
IPC2_protein 9.604
IPC_protein 10.262
Toseland 10.584
ProMoST 10.248
Dawson 10.701
Bjellqvist 10.379
Wikipedia 10.891
Rodwell 10.95
Grimsley 10.745
Solomon 10.774
Lehninger 10.745
Nozaki 10.555
DTASelect 10.379
Thurlkill 10.584
EMBOSS 10.979
Sillero 10.613
Patrickios 10.643
IPC_peptide 10.789
IPC2_peptide 9.033
IPC2.peptide.svr19 8.834
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19
4
23
8345
116
931
362.8
40.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.717 ± 0.526
1.582 ± 0.299
4.997 ± 0.256
5.848 ± 0.489
4.05 ± 0.262
6.207 ± 0.315
2.013 ± 0.267
3.751 ± 0.171
3.463 ± 0.423
9.623 ± 0.526
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.552 ± 0.228
4.985 ± 0.476
6.794 ± 0.467
4.146 ± 0.238
7.549 ± 0.8
7.01 ± 0.377
5.872 ± 0.448
6.854 ± 0.303
1.378 ± 0.098
3.607 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here