endosymbiont of Tevnia jerichonana (vent Tica)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; sulfur-oxidizing symbionts; endosymbiont of Tevnia jerichonana

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3230 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2FJL0|G2FJL0_9GAMM Electron transport complex protein RnfA OS=endosymbiont of Tevnia jerichonana (vent Tica) OX=1049564 GN=rnfA3 PE=4 SV=1
MM1 pKa = 7.51LFAGFSVFAEE11 pKa = 4.08VLVEE15 pKa = 5.48DD16 pKa = 5.37GFDD19 pKa = 3.38NDD21 pKa = 3.99SAGFTYY27 pKa = 10.71VDD29 pKa = 4.25DD30 pKa = 4.9PFHH33 pKa = 6.81GSSAPGYY40 pKa = 10.72ADD42 pKa = 3.72GSHH45 pKa = 6.74LSVGGYY51 pKa = 9.62SGGALQVILGGRR63 pKa = 11.84DD64 pKa = 3.3TIDD67 pKa = 3.41LFGLSGGWQQEE78 pKa = 4.1FEE80 pKa = 4.29LAGLSEE86 pKa = 4.33VSLSFRR92 pKa = 11.84YY93 pKa = 9.83KK94 pKa = 10.35LSQSPFYY101 pKa = 10.91EE102 pKa = 4.63GDD104 pKa = 3.35EE105 pKa = 4.17YY106 pKa = 11.68SQVLVSIDD114 pKa = 3.36GVLVGVSPNDD124 pKa = 3.74YY125 pKa = 10.01IDD127 pKa = 3.58QVSGNGNGGAEE138 pKa = 4.29EE139 pKa = 4.35TTGWRR144 pKa = 11.84QVEE147 pKa = 4.34LNLGGLAAGRR157 pKa = 11.84HH158 pKa = 4.72RR159 pKa = 11.84LIIGGYY165 pKa = 9.2NSQKK169 pKa = 8.91TAANEE174 pKa = 4.13TTEE177 pKa = 4.27VLIDD181 pKa = 4.35DD182 pKa = 5.31LLLSSGNSNLPPRR195 pKa = 11.84TVVGADD201 pKa = 3.33PTAGVAPLQVQFSSEE216 pKa = 3.92GSTDD220 pKa = 3.3PDD222 pKa = 3.35GRR224 pKa = 11.84IVGYY228 pKa = 9.74HH229 pKa = 4.86WVFGDD234 pKa = 3.97GNSSTEE240 pKa = 3.69INPLHH245 pKa = 6.88SYY247 pKa = 8.15ATAGNYY253 pKa = 7.39TVTLILTDD261 pKa = 3.73DD262 pKa = 3.49QGATGSASIMINVSGLSDD280 pKa = 3.45TAAPEE285 pKa = 3.86IPAGVSAIPSGADD298 pKa = 3.21TIQLSWQAASDD309 pKa = 3.95NVGVTGYY316 pKa = 10.65RR317 pKa = 11.84VFRR320 pKa = 11.84DD321 pKa = 3.76GVEE324 pKa = 4.11IASSTTTSYY333 pKa = 11.45RR334 pKa = 11.84DD335 pKa = 3.33TGLSPLTRR343 pKa = 11.84YY344 pKa = 9.61SYY346 pKa = 10.73RR347 pKa = 11.84VSAYY351 pKa = 9.96DD352 pKa = 3.24AAGNEE357 pKa = 4.33SEE359 pKa = 4.25PGEE362 pKa = 4.28VVAATTGNQLVPLIEE377 pKa = 4.65SGFDD381 pKa = 3.17SDD383 pKa = 4.42SAGFSYY389 pKa = 11.3VDD391 pKa = 3.37DD392 pKa = 4.41PFRR395 pKa = 11.84NTSAPGYY402 pKa = 10.85ADD404 pKa = 3.32GSYY407 pKa = 10.35LGAGGYY413 pKa = 9.56SGSALQITLGGIDD426 pKa = 3.93GDD428 pKa = 5.09DD429 pKa = 3.21IFGLSGGWQRR439 pKa = 11.84DD440 pKa = 3.85FEE442 pKa = 4.46LTSQSEE448 pKa = 4.19LSLSFRR454 pKa = 11.84YY455 pKa = 9.7RR456 pKa = 11.84LSQSPFYY463 pKa = 10.57EE464 pKa = 3.74QDD466 pKa = 3.24EE467 pKa = 4.3QSQLLVSIDD476 pKa = 3.46GVLVGVSPNDD486 pKa = 3.68YY487 pKa = 10.15IDD489 pKa = 4.02QISGNGNGGAEE500 pKa = 4.29EE501 pKa = 4.35TTGWRR506 pKa = 11.84QVALSLGSLVGGSHH520 pKa = 6.81RR521 pKa = 11.84LIIGGYY527 pKa = 9.08NNQKK531 pKa = 7.95TAANEE536 pKa = 4.14TTEE539 pKa = 4.13VLIDD543 pKa = 3.98DD544 pKa = 4.52LVLRR548 pKa = 11.84SDD550 pKa = 4.2SSNLPPSAVASAAPTAGIAPLEE572 pKa = 4.18VSFSSVGSSDD582 pKa = 3.58PDD584 pKa = 3.23GTIQHH589 pKa = 6.49FSWAFGDD596 pKa = 4.44GSSSTEE602 pKa = 3.6ANPLHH607 pKa = 7.14SYY609 pKa = 6.96TTPGDD614 pKa = 3.58YY615 pKa = 9.39TATLSVTDD623 pKa = 3.89NQGATASASVGISVSDD639 pKa = 4.29LPDD642 pKa = 3.45TEE644 pKa = 4.96APEE647 pKa = 4.31IPAGLSAIPSGSDD660 pKa = 3.18TIEE663 pKa = 4.64LSWQAASDD671 pKa = 3.95NVGVTGYY678 pKa = 10.65RR679 pKa = 11.84VFRR682 pKa = 11.84DD683 pKa = 3.76GVEE686 pKa = 4.0IASSATISYY695 pKa = 10.0RR696 pKa = 11.84DD697 pKa = 3.45SRR699 pKa = 11.84LTPSTRR705 pKa = 11.84YY706 pKa = 9.61SYY708 pKa = 10.79QISAYY713 pKa = 9.93DD714 pKa = 3.27AAGNQSEE721 pKa = 4.58PGVAVAATTGAQPTLTSLSLTPASAQLNIGLRR753 pKa = 11.84QQFNVSGFDD762 pKa = 3.28QYY764 pKa = 12.17GNTIVVNPTWSVNGGGVIDD783 pKa = 3.77STGLFSASQAGGPFTVLAEE802 pKa = 4.12QDD804 pKa = 3.7GVRR807 pKa = 11.84ATASLTVIRR816 pKa = 11.84QSNLIDD822 pKa = 3.54SGFDD826 pKa = 3.13SDD828 pKa = 4.76SAGFSYY834 pKa = 11.09LDD836 pKa = 3.51DD837 pKa = 4.37PFRR840 pKa = 11.84GSGAPAYY847 pKa = 10.5ADD849 pKa = 3.3GSYY852 pKa = 10.45LGAGGYY858 pKa = 9.84SGGALQITLGGIDD871 pKa = 3.95GDD873 pKa = 5.25DD874 pKa = 3.33IFGMSGGWQHH884 pKa = 7.26DD885 pKa = 3.77FQLNALSEE893 pKa = 4.35VSLSFRR899 pKa = 11.84YY900 pKa = 9.71QLSQTPFYY908 pKa = 10.54EE909 pKa = 4.15RR910 pKa = 11.84NEE912 pKa = 3.88YY913 pKa = 10.33SQLLLSIDD921 pKa = 3.62GVLVGVSPNDD931 pKa = 4.07FIDD934 pKa = 3.96QISGNGNGGAEE945 pKa = 4.29EE946 pKa = 4.35TTGWRR951 pKa = 11.84QVEE954 pKa = 4.35LNLGSLAAGSHH965 pKa = 5.78RR966 pKa = 11.84LIIGAYY972 pKa = 9.29NNQKK976 pKa = 7.97TAANEE981 pKa = 4.23TTVVLIDD988 pKa = 4.24DD989 pKa = 4.57LLLRR993 pKa = 11.84SSSSLPVNLPPSAVASADD1011 pKa = 3.47PQTGVVPLVVNFSSAGSTDD1030 pKa = 3.42PDD1032 pKa = 3.48GTIEE1036 pKa = 4.1HH1037 pKa = 6.31VSWAFGDD1044 pKa = 4.45GSSSTEE1050 pKa = 3.6ANPLHH1055 pKa = 7.14SYY1057 pKa = 6.96TTPGDD1062 pKa = 3.58YY1063 pKa = 9.39TATLSVTDD1071 pKa = 3.89NQGATASASVGISVSDD1087 pKa = 3.9QPDD1090 pKa = 3.53TEE1092 pKa = 4.37APEE1095 pKa = 4.27IPAGLSAIPSGSDD1108 pKa = 2.89IIEE1111 pKa = 4.62LSWQAASDD1119 pKa = 3.68NVGIIGYY1126 pKa = 9.4RR1127 pKa = 11.84VFRR1130 pKa = 11.84DD1131 pKa = 3.61GVEE1134 pKa = 3.97IANSAITSYY1143 pKa = 10.65RR1144 pKa = 11.84DD1145 pKa = 3.12SGLTPSTRR1153 pKa = 11.84YY1154 pKa = 9.67SYY1156 pKa = 10.74QVSAYY1161 pKa = 9.92DD1162 pKa = 3.33AAGNQSEE1169 pKa = 4.58PGVAVAATTGNQPSLLIEE1187 pKa = 5.19DD1188 pKa = 4.96GFDD1191 pKa = 3.2TDD1193 pKa = 3.85SAGFSYY1199 pKa = 11.22VDD1201 pKa = 3.38DD1202 pKa = 4.48PFRR1205 pKa = 11.84GSSAPGYY1212 pKa = 10.82ADD1214 pKa = 4.77GSLLPDD1220 pKa = 3.9GGYY1223 pKa = 10.36SGGALQIILGGINSADD1239 pKa = 3.03IFGLSGGWQRR1249 pKa = 11.84DD1250 pKa = 3.6FQLNGLSKK1258 pKa = 10.36VVLSFRR1264 pKa = 11.84YY1265 pKa = 9.56RR1266 pKa = 11.84LSQSPFYY1273 pKa = 10.57EE1274 pKa = 3.74QDD1276 pKa = 3.24EE1277 pKa = 4.3QSQLLVSLDD1286 pKa = 3.61GEE1288 pKa = 4.96LIGVTPNDD1296 pKa = 4.46YY1297 pKa = 10.45IDD1299 pKa = 3.59QVQGNGNGGPEE1310 pKa = 4.35EE1311 pKa = 4.32STGWRR1316 pKa = 11.84QVEE1319 pKa = 4.0LDD1321 pKa = 4.39LGNLAIGSHH1330 pKa = 5.71RR1331 pKa = 11.84QPSADD1336 pKa = 3.62HH1337 pKa = 6.99RR1338 pKa = 11.84RR1339 pKa = 11.84LQQQ1342 pKa = 3.09

Molecular weight: 139.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G2FGQ4|G2FGQ4_9GAMM Kinesin light chain-like protein OS=endosymbiont of Tevnia jerichonana (vent Tica) OX=1049564 GN=TevJSym_ar00330 PE=4 SV=1
MM1 pKa = 7.45MKK3 pKa = 9.48RR4 pKa = 11.84TFQPSNLKK12 pKa = 9.99RR13 pKa = 11.84VRR15 pKa = 11.84THH17 pKa = 6.07GFRR20 pKa = 11.84TRR22 pKa = 11.84MATQNGRR29 pKa = 11.84KK30 pKa = 9.09VIKK33 pKa = 10.14ARR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.69GRR40 pKa = 11.84ARR42 pKa = 11.84LIPP45 pKa = 4.0

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3230

0

3230

1002472

37

1870

310.4

34.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.749 ± 0.046

1.004 ± 0.02

5.507 ± 0.036

6.725 ± 0.044

3.607 ± 0.027

7.768 ± 0.041

2.323 ± 0.025

5.55 ± 0.035

3.677 ± 0.042

11.38 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.499 ± 0.021

3.101 ± 0.031

4.706 ± 0.033

4.734 ± 0.044

6.655 ± 0.044

6.054 ± 0.041

4.621 ± 0.036

6.535 ± 0.043

1.283 ± 0.018

2.523 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski