Phycicoccus elongatus Lp2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Intrasporangiaceae; Phycicoccus; Phycicoccus elongatus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3089 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|N0E3Z8|N0E3Z8_9MICO Uncharacterized protein OS=Phycicoccus elongatus Lp2 OX=1193181 GN=BN10_590063 PE=4 SV=1
MM1 pKa = 7.79SDD3 pKa = 2.97ASYY6 pKa = 8.54PTDD9 pKa = 3.3LRR11 pKa = 11.84YY12 pKa = 7.99TAEE15 pKa = 4.24HH16 pKa = 6.38EE17 pKa = 4.59WVKK20 pKa = 10.2STGEE24 pKa = 3.77DD25 pKa = 3.29TVRR28 pKa = 11.84VGITSFAQEE37 pKa = 3.74ALGDD41 pKa = 3.94VVYY44 pKa = 10.94VSAPSIGDD52 pKa = 3.29AVTAGDD58 pKa = 3.41SCGEE62 pKa = 4.14VEE64 pKa = 4.44STKK67 pKa = 10.7SVSDD71 pKa = 4.17LYY73 pKa = 11.45SPLSGEE79 pKa = 4.23VVAVNDD85 pKa = 4.53ALDD88 pKa = 3.88ATPEE92 pKa = 4.17LVNSDD97 pKa = 4.17PYY99 pKa = 10.17GDD101 pKa = 3.22GWIFEE106 pKa = 4.44VRR108 pKa = 11.84VSDD111 pKa = 3.84PATIDD116 pKa = 3.34GLMDD120 pKa = 3.56ATAYY124 pKa = 9.73EE125 pKa = 4.48GQLGG129 pKa = 3.55

Molecular weight: 13.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N0DY06|N0DY06_9MICO Phosphate-binding protein PstS OS=Phycicoccus elongatus Lp2 OX=1193181 GN=pstS PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3089

0

3089

952598

13

1870

308.4

33.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.11 ± 0.063

0.724 ± 0.013

6.277 ± 0.041

5.426 ± 0.042

2.778 ± 0.025

9.164 ± 0.038

2.356 ± 0.021

3.974 ± 0.03

2.16 ± 0.037

10.163 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.974 ± 0.016

1.804 ± 0.023

5.622 ± 0.038

2.739 ± 0.023

7.71 ± 0.051

5.358 ± 0.029

6.146 ± 0.035

9.157 ± 0.042

1.585 ± 0.021

1.773 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski