Luteimonas cucumeris
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3213 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A562KUE3|A0A562KUE3_9GAMM Uncharacterized protein DUF4177 OS=Luteimonas cucumeris OX=985012 GN=IP90_03228 PE=4 SV=1
MM1 pKa = 7.35 NKK3 pKa = 9.03 VFSLVWSRR11 pKa = 11.84 SLQALVVASEE21 pKa = 4.13 LASANGRR28 pKa = 11.84 KK29 pKa = 9.61 AGRR32 pKa = 11.84 PRR34 pKa = 11.84 RR35 pKa = 11.84 LAHH38 pKa = 6.19 AAFGVVVLSASFSLAPLAQAAEE60 pKa = 4.47 SGKK63 pKa = 8.05 PTCTVNLPGQAGVAVDD79 pKa = 4.46 CAQLQQIVATAQRR92 pKa = 11.84 AGDD95 pKa = 3.66 PRR97 pKa = 11.84 KK98 pKa = 9.98 GRR100 pKa = 11.84 KK101 pKa = 8.66 ARR103 pKa = 11.84 PVVDD107 pKa = 5.12 SFDD110 pKa = 3.63 SDD112 pKa = 3.84 YY113 pKa = 11.44 FKK115 pKa = 11.52 ANGTGDD121 pKa = 3.88 GDD123 pKa = 3.83 DD124 pKa = 4.95 ASAQGEE130 pKa = 4.21 GAIAIGSGAQTRR142 pKa = 11.84 PWVSDD147 pKa = 3.22 EE148 pKa = 4.27 LGEE151 pKa = 4.35 SFEE154 pKa = 4.92 AEE156 pKa = 3.79 GAVAIGRR163 pKa = 11.84 QSVAEE168 pKa = 4.25 GWEE171 pKa = 4.11 ATALGANSFASSNSATAVGGEE192 pKa = 4.19 SGAFGIGSSAFGQGSKK208 pKa = 11.0 AFGHH212 pKa = 6.93 AATALGQGAMTFGFGNTAVGASAMASAEE240 pKa = 4.02 QAATALGALSEE251 pKa = 4.16 AWSNGSTAVGGMAIAAGEE269 pKa = 4.22 GSLAAGFDD277 pKa = 3.79 SQAGGFEE284 pKa = 4.42 SVALGGHH291 pKa = 4.75 TRR293 pKa = 11.84 VGNGEE298 pKa = 4.05 AGVGVGANAVALGNGAVGVGNNSLANGSNTVAMGRR333 pKa = 11.84 NAAANSEE340 pKa = 4.1 GALAIGGSEE349 pKa = 4.03 WTDD352 pKa = 3.02 HH353 pKa = 7.32 DD354 pKa = 5.0 GDD356 pKa = 3.89 GMPDD360 pKa = 3.08 VGEE363 pKa = 4.07 ATLAEE368 pKa = 4.42 GQNAVAIGGLAGASGDD384 pKa = 3.45 NAQAIGHH391 pKa = 5.58 QANALAEE398 pKa = 4.2 NATATGAMSMAAGEE412 pKa = 4.24 NSTATGASSVANGIDD427 pKa = 3.2 SSAYY431 pKa = 9.48 GAQSQADD438 pKa = 4.12 GDD440 pKa = 3.94 HH441 pKa = 5.95 STAIGSGSLALGVGSSAVGSGANAFGEE468 pKa = 4.29 YY469 pKa = 10.14 SVANGYY475 pKa = 10.26 GSAAEE480 pKa = 4.33 GADD483 pKa = 3.15 AVAVGSNSAASGSGASALGAAAQAVGTQSAAMGFNSQALEE523 pKa = 4.03 MATTAVGGQTVANQWFATAIGYY545 pKa = 8.85 GSQATGYY552 pKa = 10.27 SSVALGEE559 pKa = 4.31 NVLATGEE566 pKa = 3.93 MSIAIGNSWAGMMQTQSNGNMSVAIGSAAATWDD599 pKa = 3.57 NYY601 pKa = 9.51 GVAVGSLAGANTEE614 pKa = 4.01 YY615 pKa = 9.6 STSVGAMSAAIGQQSTALGAAAMAEE640 pKa = 4.19 ADD642 pKa = 3.75 DD643 pKa = 4.4 SVAIGYY649 pKa = 8.99 YY650 pKa = 9.87 SHH652 pKa = 7.34 ASGANSVALGNEE664 pKa = 4.43 SVASRR669 pKa = 11.84 DD670 pKa = 3.43 NTISVGKK677 pKa = 10.17 AGSEE681 pKa = 3.76 RR682 pKa = 11.84 QITNVAAGTEE692 pKa = 4.09 ATDD695 pKa = 4.07 AVNKK699 pKa = 10.14 AQLDD703 pKa = 4.02 EE704 pKa = 4.44 VATGVADD711 pKa = 3.93 ANHH714 pKa = 5.87 YY715 pKa = 9.26 FAANGAADD723 pKa = 4.09 GSDD726 pKa = 3.4 DD727 pKa = 3.8 AMASGDD733 pKa = 3.74 HH734 pKa = 6.32 AVATGAYY741 pKa = 8.86 AVANGIEE748 pKa = 4.2 SSAYY752 pKa = 9.91 GYY754 pKa = 10.39 QSQADD759 pKa = 3.72 GDD761 pKa = 4.06 YY762 pKa = 10.72 SQAIGSGSLAMGVGTSAVGSGANAIGNGASAFGAGAVAFLDD803 pKa = 4.06 GATAVGSNAQTAGLNTVAVGNGAAASATGAVAIGGQATNGLGQPILADD851 pKa = 3.79 GGTSEE856 pKa = 5.03 PLGAAQAAEE865 pKa = 4.25 LSTAVGASTYY875 pKa = 10.17 ATGNWSAAYY884 pKa = 9.7 GVGAWSAGSQSTAVGALAIAYY905 pKa = 8.21 GDD907 pKa = 3.68 SSSAFGTNTMASGQSSTAIGQSAWALGDD935 pKa = 3.42 YY936 pKa = 10.57 SVAIGVAANTSGSNTVAIGNSATVFDD962 pKa = 4.38 YY963 pKa = 9.75 STWSNVSDD971 pKa = 3.65 AVALGSASMASADD984 pKa = 3.53 HH985 pKa = 6.65 AVALGSNSHH994 pKa = 6.16 AMADD998 pKa = 3.44 NSVALGQGSLADD1010 pKa = 3.9 RR1011 pKa = 11.84 ANTVSVGAAEE1021 pKa = 4.17 AWTDD1025 pKa = 3.7 LLGTSHH1031 pKa = 6.68 EE1032 pKa = 4.76 AIDD1035 pKa = 3.76 RR1036 pKa = 11.84 QITNVAAGTEE1046 pKa = 4.09 ATDD1049 pKa = 4.99 AVNKK1053 pKa = 10.24 DD1054 pKa = 3.52 QLDD1057 pKa = 3.67 DD1058 pKa = 3.38 ATRR1061 pKa = 11.84 YY1062 pKa = 10.34 LEE1064 pKa = 4.59 ANGAGDD1070 pKa = 3.92 GSDD1073 pKa = 3.77 DD1074 pKa = 4.17 AVATGQYY1081 pKa = 8.6 ATATGANSIANGAEE1095 pKa = 3.83 SSVYY1099 pKa = 10.3 GYY1101 pKa = 10.58 QSQADD1106 pKa = 3.72 GDD1108 pKa = 4.17 YY1109 pKa = 9.56 ATAIGSSSYY1118 pKa = 11.31 AGALGANAIGTGATALGEE1136 pKa = 4.11 TSNAIGYY1143 pKa = 9.85 GSVATGLDD1151 pKa = 3.29 AVAVGTSSNASGVNALAMGAGSSASADD1178 pKa = 3.11 VSVAINTGSVAGLWGVAPGVNAAGGVGIGAAAVVGANADD1217 pKa = 3.46 AGVALGLSATVVEE1230 pKa = 4.89 GAYY1233 pKa = 10.04 QGVAIGMGSYY1243 pKa = 10.46 VGDD1246 pKa = 3.68 AQGIAIGGFGARR1258 pKa = 11.84 FIDD1261 pKa = 4.42 GSIEE1265 pKa = 3.91 LRR1267 pKa = 11.84 PTVAMGTGAVAIGLGSEE1284 pKa = 4.42 TFNDD1288 pKa = 3.36 HH1289 pKa = 5.65 ATALGAFSTVTAQNAVAIGANSLADD1314 pKa = 4.03 RR1315 pKa = 11.84 DD1316 pKa = 3.96 NTLSVGYY1323 pKa = 9.92 ADD1325 pKa = 4.8 HH1326 pKa = 6.95 EE1327 pKa = 4.25 RR1328 pKa = 11.84 QITHH1332 pKa = 5.84 VAAGTEE1338 pKa = 4.03 ATDD1341 pKa = 4.99 AVNKK1345 pKa = 10.24 DD1346 pKa = 3.52 QLDD1349 pKa = 3.68 DD1350 pKa = 3.5 ATRR1353 pKa = 11.84 YY1354 pKa = 8.88 LASNGAGDD1362 pKa = 4.17 GSDD1365 pKa = 3.84 DD1366 pKa = 4.07 AVATGDD1372 pKa = 3.48 QAVATGASAVANGVGSSAYY1391 pKa = 9.97 GSQGYY1396 pKa = 10.99 ADD1398 pKa = 4.52 GDD1400 pKa = 3.85 YY1401 pKa = 10.04 STAVGSGSLALGAGASAFGSGANALGEE1428 pKa = 4.12 NSTALGTGALASGFGAYY1445 pKa = 10.5 AMGNASNAAARR1456 pKa = 11.84 NSLALGGMASVDD1468 pKa = 3.4 QSSALGTAIGYY1479 pKa = 7.92 GAYY1482 pKa = 9.54 IGANSSAAIAMGFAAGVGDD1501 pKa = 3.88 GASNSISMGNLSYY1514 pKa = 11.33 ADD1516 pKa = 3.8 SPYY1519 pKa = 10.31 TIAIGSMFAYY1529 pKa = 10.23 VNHH1532 pKa = 7.39 DD1533 pKa = 3.55 GGLGLATTGASGWGAVAFGPGAQALGDD1560 pKa = 3.74 YY1561 pKa = 9.55 TLALGPAASVTAANAVALGGLSIADD1586 pKa = 4.05 RR1587 pKa = 11.84 EE1588 pKa = 4.45 NTVSVGAADD1597 pKa = 4.1 HH1598 pKa = 6.29 EE1599 pKa = 4.32 RR1600 pKa = 11.84 QIVNVAAGTEE1610 pKa = 4.0 ATDD1613 pKa = 3.73 AVNVAQLDD1621 pKa = 3.73 DD1622 pKa = 3.47 ATRR1625 pKa = 11.84 YY1626 pKa = 9.3 VAVNGAGDD1634 pKa = 3.82 GSDD1637 pKa = 3.74 DD1638 pKa = 4.31 AVATGEE1644 pKa = 4.09 NATATGASSVANGVEE1659 pKa = 4.09 SSAYY1663 pKa = 10.03 GSQSYY1668 pKa = 11.5 ADD1670 pKa = 4.09 ADD1672 pKa = 3.75 YY1673 pKa = 10.0 STALGSNSLALGIGASAIGSGANALGTNSVANGYY1707 pKa = 10.91 ASVASGEE1714 pKa = 4.03 LSIAQGSEE1722 pKa = 3.84 SQALGEE1728 pKa = 4.03 ASIALGTQSLAEE1740 pKa = 5.08 GIDD1743 pKa = 3.92 SIGVGAGSYY1752 pKa = 10.67 ASVYY1756 pKa = 8.0 GTAVGTLSQAGMYY1769 pKa = 10.44 GSTALGHH1776 pKa = 6.2 SATANNDD1783 pKa = 2.86 FALALGAVSTSLAVGGIAIGTEE1805 pKa = 4.22 SYY1807 pKa = 11.57 VDD1809 pKa = 3.32 ANAYY1813 pKa = 9.37 QGIAIGSVVSAIGHH1827 pKa = 5.92 RR1828 pKa = 11.84 SIAIGSQWTTAGGDD1842 pKa = 3.52 SAVAIGGTMACSGICIEE1859 pKa = 4.74 QNNAAWGANSVALGAGTQAQADD1881 pKa = 4.18 GATALGAGANAGGIGSVAAGQFAQAWSDD1909 pKa = 3.65 DD1910 pKa = 3.47 AVALGNTSQAIAAGSVALGAYY1931 pKa = 9.42 SVADD1935 pKa = 3.81 RR1936 pKa = 11.84 DD1937 pKa = 3.99 NTVSVGDD1944 pKa = 4.08 AGTGLTRR1951 pKa = 11.84 QITNVAAGTEE1961 pKa = 4.18 ANDD1964 pKa = 4.01 AVNKK1968 pKa = 9.86 GQLDD1972 pKa = 3.96 EE1973 pKa = 5.34 LSDD1976 pKa = 4.3 SIGDD1980 pKa = 3.62 ANYY1983 pKa = 10.73 YY1984 pKa = 9.66 FAADD1988 pKa = 3.85 GAGDD1992 pKa = 3.88 GSDD1995 pKa = 3.25 AAVASGVGSVAAGANSVASGTGTVAYY2021 pKa = 10.16 GRR2023 pKa = 11.84 GALASGEE2030 pKa = 3.96 QAMAIGDD2037 pKa = 4.11 GAQADD2042 pKa = 4.32 YY2043 pKa = 9.88 FYY2045 pKa = 11.75 AMAIGAGAVSSSWASMAIGRR2065 pKa = 11.84 NAIASGPRR2073 pKa = 11.84 AVAIGEE2079 pKa = 4.1 NAQAMDD2085 pKa = 4.11 NGNVALGWASQSVGMFSAALGGSSAAIGWNTLAAGFGAWVLGNNATAVGAGAGSFYY2141 pKa = 10.66 TVDD2144 pKa = 3.97 EE2145 pKa = 4.24 ITTVGSNARR2154 pKa = 11.84 ADD2156 pKa = 3.79 SFRR2159 pKa = 11.84 GTVVGAGSVAAEE2171 pKa = 4.09 WAPEE2175 pKa = 4.0 STIVGANAFSFDD2187 pKa = 4.17 SNGTAIGEE2195 pKa = 4.32 SSQVSYY2201 pKa = 11.02 GAANAVALGSGSHH2214 pKa = 7.05 ADD2216 pKa = 3.66 RR2217 pKa = 11.84 ANTVSVGASGPWTDD2231 pKa = 3.43 GFGVEE2236 pKa = 4.82 HH2237 pKa = 7.46 PALQRR2242 pKa = 11.84 QITNVAAGTEE2252 pKa = 3.99 ASDD2255 pKa = 4.44 AVNKK2259 pKa = 10.29 AQLDD2263 pKa = 3.88 EE2264 pKa = 4.15 VAAAGEE2270 pKa = 4.13 ATGKK2274 pKa = 10.35 FFF2276 pKa = 5.64
Molecular weight: 216.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.821
IPC_protein 3.872
Toseland 3.643
ProMoST 4.024
Dawson 3.859
Bjellqvist 3.999
Wikipedia 3.783
Rodwell 3.694
Grimsley 3.554
Solomon 3.859
Lehninger 3.808
Nozaki 3.961
DTASelect 4.215
Thurlkill 3.681
EMBOSS 3.795
Sillero 3.986
Patrickios 0.82
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.87
Protein with the highest isoelectric point:
>tr|A0A562LFD5|A0A562LFD5_9GAMM TetR family transcriptional regulator OS=Luteimonas cucumeris OX=985012 GN=IP90_00586 PE=4 SV=1
MM1 pKa = 7.41 AVKK4 pKa = 10.23 KK5 pKa = 10.04 AAKK8 pKa = 9.47 KK9 pKa = 10.42 RR10 pKa = 11.84 PAKK13 pKa = 10.19 KK14 pKa = 9.78 AAKK17 pKa = 8.48 KK18 pKa = 7.32 TAKK21 pKa = 9.03 KK22 pKa = 6.66 TTAKK26 pKa = 10.32 KK27 pKa = 9.12 ATRR30 pKa = 11.84 KK31 pKa = 8.48 VAKK34 pKa = 9.92 KK35 pKa = 10.08 AARR38 pKa = 11.84 KK39 pKa = 7.18 PAKK42 pKa = 9.89 KK43 pKa = 9.97 AVRR46 pKa = 11.84 KK47 pKa = 8.88 AAKK50 pKa = 10.02 KK51 pKa = 9.89 KK52 pKa = 8.43 VAKK55 pKa = 9.97 KK56 pKa = 10.19 AVRR59 pKa = 11.84 KK60 pKa = 8.84 AVKK63 pKa = 10.2 RR64 pKa = 11.84 KK65 pKa = 8.03 VAKK68 pKa = 10.1 KK69 pKa = 9.16 AAKK72 pKa = 9.71 KK73 pKa = 10.15 AVRR76 pKa = 11.84 KK77 pKa = 8.9 AAKK80 pKa = 10.02 KK81 pKa = 9.89 KK82 pKa = 8.43 VAKK85 pKa = 10.07 KK86 pKa = 10.23 AVRR89 pKa = 11.84 KK90 pKa = 8.72 VAKK93 pKa = 10.31 KK94 pKa = 10.02 KK95 pKa = 8.89 VAKK98 pKa = 10.27 KK99 pKa = 10.06 AVKK102 pKa = 9.9 KK103 pKa = 10.01 AAKK106 pKa = 9.87 KK107 pKa = 8.93 KK108 pKa = 7.13 TAKK111 pKa = 8.99 KK112 pKa = 7.45 TAKK115 pKa = 10.09 KK116 pKa = 9.81 AAKK119 pKa = 9.34 KK120 pKa = 10.09 AKK122 pKa = 9.71 KK123 pKa = 10.02 KK124 pKa = 8.23 PARR127 pKa = 11.84 RR128 pKa = 11.84 AKK130 pKa = 9.82 KK131 pKa = 9.68 AAPATMPAMPAPMII145 pKa = 4.36
Molecular weight: 15.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.451
IPC2_protein 11.418
IPC_protein 12.793
Toseland 13.027
ProMoST 13.466
Dawson 13.042
Bjellqvist 12.983
Wikipedia 13.466
Rodwell 13.334
Grimsley 13.071
Solomon 13.481
Lehninger 13.393
Nozaki 13.027
DTASelect 12.983
Thurlkill 13.027
EMBOSS 13.51
Sillero 13.027
Patrickios 13.042
IPC_peptide 13.495
IPC2_peptide 12.442
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3213
0
3213
1045802
39
2311
325.5
35.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.061 ± 0.079
0.801 ± 0.014
6.124 ± 0.033
5.305 ± 0.041
3.391 ± 0.027
8.55 ± 0.055
2.231 ± 0.022
4.324 ± 0.027
2.796 ± 0.038
10.616 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.207 ± 0.017
2.554 ± 0.028
5.214 ± 0.037
3.786 ± 0.028
7.678 ± 0.052
5.315 ± 0.036
4.921 ± 0.035
7.26 ± 0.037
1.516 ± 0.019
2.349 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here