Penicillium patulum (Penicillium griseofulvum)
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9628 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A135M0J1|A0A135M0J1_PENPA Uncharacterized protein OS=Penicillium patulum OX=5078 GN=PGRI_078720 PE=4 SV=1
MM1 pKa = 7.43 ATLDD5 pKa = 4.37 CGTDD9 pKa = 3.28 ADD11 pKa = 4.56 GSDD14 pKa = 3.21 NGSDD18 pKa = 3.24 EE19 pKa = 4.45 KK20 pKa = 11.03 EE21 pKa = 4.53 GYY23 pKa = 10.02 FSAEE27 pKa = 4.15 EE28 pKa = 3.92 FLSPSPYY35 pKa = 10.05 LRR37 pKa = 11.84 PATPQLYY44 pKa = 9.66 YY45 pKa = 9.8 EE46 pKa = 4.42 QHH48 pKa = 6.0 PAWWPVSDD56 pKa = 5.55 DD57 pKa = 3.7 NNFDD61 pKa = 3.66 LTRR64 pKa = 11.84 TCDD67 pKa = 3.4 PVDD70 pKa = 3.8 PVSMRR75 pKa = 11.84 RR76 pKa = 11.84 DD77 pKa = 3.29 YY78 pKa = 10.76 RR79 pKa = 11.84 ARR81 pKa = 11.84 RR82 pKa = 11.84 PKK84 pKa = 8.12 TTPSQYY90 pKa = 11.08 SSDD93 pKa = 3.77 DD94 pKa = 3.18 TDD96 pKa = 4.4 SFVSCEE102 pKa = 3.93 EE103 pKa = 4.07 YY104 pKa = 10.89 LDD106 pKa = 4.73 EE107 pKa = 5.47 EE108 pKa = 4.47 NPDD111 pKa = 3.77 DD112 pKa = 5.72 KK113 pKa = 11.26 DD114 pKa = 3.64 YY115 pKa = 11.39 CSRR118 pKa = 11.84 NGEE121 pKa = 3.92 YY122 pKa = 10.74 DD123 pKa = 3.2 LL124 pKa = 5.98
Molecular weight: 14.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.897
IPC_protein 3.897
Toseland 3.668
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.834
Rodwell 3.719
Grimsley 3.579
Solomon 3.884
Lehninger 3.834
Nozaki 3.999
DTASelect 4.253
Thurlkill 3.732
EMBOSS 3.834
Sillero 4.012
Patrickios 1.1
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.896
Protein with the highest isoelectric point:
>tr|A0A135LUK8|A0A135LUK8_PENPA Uncharacterized protein OS=Penicillium patulum OX=5078 GN=PGRI_079010 PE=4 SV=1
MM1 pKa = 6.97 FCLRR5 pKa = 11.84 ARR7 pKa = 11.84 AVPSTFRR14 pKa = 11.84 ATTSAMRR21 pKa = 11.84 TSMSRR26 pKa = 11.84 FAQIPQQPSTPSTMLSSRR44 pKa = 11.84 SFSSLLSTPTRR55 pKa = 11.84 FQPSRR60 pKa = 11.84 TLGARR65 pKa = 11.84 LSSPITATASAFSPLSSLLSQKK87 pKa = 9.18 TPSQSRR93 pKa = 11.84 SFSASASLGVRR104 pKa = 11.84 RR105 pKa = 11.84 VTFRR109 pKa = 11.84 PSRR112 pKa = 11.84 RR113 pKa = 11.84 VQKK116 pKa = 10.3 RR117 pKa = 11.84 RR118 pKa = 11.84 HH119 pKa = 5.75 GYY121 pKa = 9.09 LARR124 pKa = 11.84 KK125 pKa = 9.08 KK126 pKa = 10.39 DD127 pKa = 3.6 RR128 pKa = 11.84 NGRR131 pKa = 11.84 KK132 pKa = 6.83 TLIRR136 pKa = 11.84 RR137 pKa = 11.84 TLKK140 pKa = 10.34 GRR142 pKa = 11.84 KK143 pKa = 7.8 EE144 pKa = 4.02 LSWW147 pKa = 4.66
Molecular weight: 16.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.379
IPC2_protein 10.891
IPC_protein 12.398
Toseland 12.559
ProMoST 13.056
Dawson 12.559
Bjellqvist 12.559
Wikipedia 13.042
Rodwell 12.193
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 11.915
IPC_peptide 13.056
IPC2_peptide 12.047
IPC2.peptide.svr19 9.116
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9628
0
9628
5009552
66
7334
520.3
57.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.392 ± 0.018
1.175 ± 0.009
5.735 ± 0.017
6.212 ± 0.029
3.802 ± 0.017
6.756 ± 0.023
2.412 ± 0.011
5.169 ± 0.019
4.74 ± 0.019
8.95 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.267 ± 0.009
3.765 ± 0.012
6.043 ± 0.03
4.023 ± 0.016
5.884 ± 0.022
8.284 ± 0.028
5.988 ± 0.017
6.144 ± 0.018
1.463 ± 0.01
2.797 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here