Methyloversatilis sp. RAC08

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Sterolibacteriaceae; Methyloversatilis; unclassified Methyloversatilis

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3549 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B3LIY5|A0A1B3LIY5_9PROT Glycosyl transferases group 1 family protein OS=Methyloversatilis sp. RAC08 OX=1842540 GN=BSY238_1070 PE=4 SV=1
MM1 pKa = 7.06NHH3 pKa = 6.84LLKK6 pKa = 10.78SLLIAASLISAPAAFAASPVTPVSASNNGIVLIDD40 pKa = 3.98GADD43 pKa = 3.6LSILFDD49 pKa = 4.19GYY51 pKa = 10.18IAPEE55 pKa = 3.98GTPYY59 pKa = 9.17QTDD62 pKa = 3.22AVSWFGQVGSSGVTLDD78 pKa = 3.67FDD80 pKa = 4.81FGQLYY85 pKa = 8.56TLSDD89 pKa = 3.46VLIGVDD95 pKa = 4.22HH96 pKa = 7.05NDD98 pKa = 3.7FYY100 pKa = 11.48QVQVSLDD107 pKa = 3.44GSVWNTLFTNLSFEE121 pKa = 4.29GQSNFGSEE129 pKa = 4.56IISSVAGDD137 pKa = 4.13PEE139 pKa = 4.22YY140 pKa = 10.82QATIDD145 pKa = 3.9FPTTVARR152 pKa = 11.84YY153 pKa = 9.12ARR155 pKa = 11.84IYY157 pKa = 11.04AVGGDD162 pKa = 3.68SAFSVSEE169 pKa = 3.95VSFSGVAVVPEE180 pKa = 4.26PEE182 pKa = 3.77TWALLIAGLGLIGAIAYY199 pKa = 8.05RR200 pKa = 11.84RR201 pKa = 11.84SS202 pKa = 3.18

Molecular weight: 21.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B3LSN2|A0A1B3LSN2_9PROT Metallo-beta-lactamase superfamily protein OS=Methyloversatilis sp. RAC08 OX=1842540 GN=BSY238_988 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.37RR3 pKa = 11.84TYY5 pKa = 10.18QPSSVRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 6.11GFLEE20 pKa = 4.18RR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.35GRR39 pKa = 11.84VRR41 pKa = 11.84LAVV44 pKa = 3.26

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3549

0

3549

1184287

30

6884

333.7

36.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.681 ± 0.067

0.993 ± 0.014

5.996 ± 0.034

5.336 ± 0.048

3.535 ± 0.023

8.322 ± 0.069

2.238 ± 0.022

4.576 ± 0.033

2.771 ± 0.038

10.874 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.426 ± 0.026

2.52 ± 0.038

5.033 ± 0.044

3.42 ± 0.024

7.522 ± 0.048

5.586 ± 0.045

5.125 ± 0.046

7.528 ± 0.033

1.377 ± 0.02

2.142 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski