Amino acid dipepetide frequency for Methyloversatilis sp. RAC08

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.275AlaAla: 17.275 ± 0.192
1.4AlaCys: 1.4 ± 0.039
7.556AlaAsp: 7.556 ± 0.079
6.461AlaGlu: 6.461 ± 0.084
3.986AlaPhe: 3.986 ± 0.067
11.29AlaGly: 11.29 ± 0.161
2.743AlaHis: 2.743 ± 0.055
5.214AlaIle: 5.214 ± 0.073
2.983AlaLys: 2.983 ± 0.082
15.714AlaLeu: 15.714 ± 0.188
3.192AlaMet: 3.192 ± 0.063
2.663AlaAsn: 2.663 ± 0.066
5.885AlaPro: 5.885 ± 0.095
5.016AlaGln: 5.016 ± 0.088
10.258AlaArg: 10.258 ± 0.123
6.82AlaSer: 6.82 ± 0.099
5.906AlaThr: 5.906 ± 0.091
8.466AlaVal: 8.466 ± 0.09
1.727AlaTrp: 1.727 ± 0.046
2.257AlaTyr: 2.257 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
1.388CysAla: 1.388 ± 0.037
0.152CysCys: 0.152 ± 0.012
0.649CysAsp: 0.649 ± 0.028
0.535CysGlu: 0.535 ± 0.023
0.318CysPhe: 0.318 ± 0.019
1.046CysGly: 1.046 ± 0.033
0.291CysHis: 0.291 ± 0.016
0.432CysIle: 0.432 ± 0.017
0.179CysLys: 0.179 ± 0.012
0.853CysLeu: 0.853 ± 0.027
0.233CysMet: 0.233 ± 0.015
0.252CysAsn: 0.252 ± 0.013
0.507CysPro: 0.507 ± 0.024
0.209CysGln: 0.209 ± 0.013
0.693CysArg: 0.693 ± 0.024
0.614CysSer: 0.614 ± 0.022
0.474CysThr: 0.474 ± 0.023
0.772CysVal: 0.772 ± 0.028
0.122CysTrp: 0.122 ± 0.011
0.206CysTyr: 0.206 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
8.614AspAla: 8.614 ± 0.106
0.558AspCys: 0.558 ± 0.022
3.628AspAsp: 3.628 ± 0.06
3.575AspGlu: 3.575 ± 0.063
2.288AspPhe: 2.288 ± 0.046
5.272AspGly: 5.272 ± 0.08
1.197AspHis: 1.197 ± 0.031
3.288AspIle: 3.288 ± 0.057
1.468AspLys: 1.468 ± 0.04
5.865AspLeu: 5.865 ± 0.066
1.43AspMet: 1.43 ± 0.039
1.355AspAsn: 1.355 ± 0.036
3.001AspPro: 3.001 ± 0.057
1.637AspGln: 1.637 ± 0.034
3.913AspArg: 3.913 ± 0.064
2.695AspSer: 2.695 ± 0.052
3.169AspThr: 3.169 ± 0.059
4.431AspVal: 4.431 ± 0.062
1.05AspTrp: 1.05 ± 0.029
1.523AspTyr: 1.523 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
6.955GluAla: 6.955 ± 0.088
0.431GluCys: 0.431 ± 0.021
2.413GluAsp: 2.413 ± 0.049
2.407GluGlu: 2.407 ± 0.058
1.838GluPhe: 1.838 ± 0.039
3.73GluGly: 3.73 ± 0.063
1.369GluHis: 1.369 ± 0.036
2.51GluIle: 2.51 ± 0.051
1.76GluLys: 1.76 ± 0.049
6.083GluLeu: 6.083 ± 0.085
1.204GluMet: 1.204 ± 0.033
1.283GluAsn: 1.283 ± 0.033
2.568GluPro: 2.568 ± 0.048
2.504GluGln: 2.504 ± 0.055
4.995GluArg: 4.995 ± 0.081
2.779GluSer: 2.779 ± 0.044
2.547GluThr: 2.547 ± 0.047
4.117GluVal: 4.117 ± 0.059
0.768GluTrp: 0.768 ± 0.025
1.098GluTyr: 1.098 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
4.355PheAla: 4.355 ± 0.064
0.377PheCys: 0.377 ± 0.018
2.8PheAsp: 2.8 ± 0.052
2.228PheGlu: 2.228 ± 0.046
1.365PhePhe: 1.365 ± 0.043
3.433PheGly: 3.433 ± 0.066
0.755PheHis: 0.755 ± 0.027
1.672PheIle: 1.672 ± 0.035
0.901PheLys: 0.901 ± 0.032
2.895PheLeu: 2.895 ± 0.051
0.81PheMet: 0.81 ± 0.029
1.146PheAsn: 1.146 ± 0.052
1.419PhePro: 1.419 ± 0.038
0.898PheGln: 0.898 ± 0.031
2.221PheArg: 2.221 ± 0.049
2.333PheSer: 2.333 ± 0.046
1.73PheThr: 1.73 ± 0.044
2.774PheVal: 2.774 ± 0.057
0.45PheTrp: 0.45 ± 0.022
0.791PheTyr: 0.791 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
9.213GlyAla: 9.213 ± 0.132
1.012GlyCys: 1.012 ± 0.038
4.769GlyAsp: 4.769 ± 0.076
4.707GlyGlu: 4.707 ± 0.063
3.416GlyPhe: 3.416 ± 0.057
7.023GlyGly: 7.023 ± 0.189
1.957GlyHis: 1.957 ± 0.046
3.905GlyIle: 3.905 ± 0.059
2.795GlyLys: 2.795 ± 0.06
8.742GlyLeu: 8.742 ± 0.096
2.287GlyMet: 2.287 ± 0.045
2.226GlyAsn: 2.226 ± 0.096
2.717GlyPro: 2.717 ± 0.057
2.846GlyGln: 2.846 ± 0.048
6.194GlyArg: 6.194 ± 0.074
4.901GlySer: 4.901 ± 0.155
4.403GlyThr: 4.403 ± 0.192
6.579GlyVal: 6.579 ± 0.08
1.363GlyTrp: 1.363 ± 0.038
2.167GlyTyr: 2.167 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
3.014HisAla: 3.014 ± 0.056
0.288HisCys: 0.288 ± 0.017
1.327HisAsp: 1.327 ± 0.034
1.173HisGlu: 1.173 ± 0.031
0.922HisPhe: 0.922 ± 0.029
2.053HisGly: 2.053 ± 0.042
0.643HisHis: 0.643 ± 0.028
0.985HisIle: 0.985 ± 0.031
0.448HisLys: 0.448 ± 0.021
2.241HisLeu: 2.241 ± 0.043
0.46HisMet: 0.46 ± 0.018
0.469HisAsn: 0.469 ± 0.02
1.427HisPro: 1.427 ± 0.036
0.66HisGln: 0.66 ± 0.026
1.563HisArg: 1.563 ± 0.041
1.083HisSer: 1.083 ± 0.032
1.043HisThr: 1.043 ± 0.032
1.619HisVal: 1.619 ± 0.037
0.347HisTrp: 0.347 ± 0.018
0.619HisTyr: 0.619 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
6.546IleAla: 6.546 ± 0.08
0.41IleCys: 0.41 ± 0.016
3.893IleAsp: 3.893 ± 0.067
3.668IleGlu: 3.668 ± 0.057
1.19IlePhe: 1.19 ± 0.037
4.549IleGly: 4.549 ± 0.076
0.925IleHis: 0.925 ± 0.03
1.647IleIle: 1.647 ± 0.048
1.355IleLys: 1.355 ± 0.042
3.253IleLeu: 3.253 ± 0.061
0.808IleMet: 0.808 ± 0.026
1.473IleAsn: 1.473 ± 0.044
2.03IlePro: 2.03 ± 0.041
1.123IleGln: 1.123 ± 0.032
2.951IleArg: 2.951 ± 0.052
2.396IleSer: 2.396 ± 0.062
2.481IleThr: 2.481 ± 0.052
3.678IleVal: 3.678 ± 0.061
0.439IleTrp: 0.439 ± 0.019
0.947IleTyr: 0.947 ± 0.028
0.0IleXaa: 0.0 ± 0.0
Lys
3.15LysAla: 3.15 ± 0.074
0.184LysCys: 0.184 ± 0.012
1.445LysAsp: 1.445 ± 0.052
1.231LysGlu: 1.231 ± 0.041
0.799LysPhe: 0.799 ± 0.028
1.988LysGly: 1.988 ± 0.046
0.595LysHis: 0.595 ± 0.026
1.294LysIle: 1.294 ± 0.044
1.141LysLys: 1.141 ± 0.043
3.259LysLeu: 3.259 ± 0.06
0.726LysMet: 0.726 ± 0.025
0.757LysAsn: 0.757 ± 0.029
1.895LysPro: 1.895 ± 0.045
1.039LysGln: 1.039 ± 0.032
1.97LysArg: 1.97 ± 0.039
1.606LysSer: 1.606 ± 0.038
1.495LysThr: 1.495 ± 0.041
2.213LysVal: 2.213 ± 0.057
0.325LysTrp: 0.325 ± 0.018
0.595LysTyr: 0.595 ± 0.025
0.0LysXaa: 0.0 ± 0.0
Leu
14.41LeuAla: 14.41 ± 0.163
1.139LeuCys: 1.139 ± 0.038
6.978LeuAsp: 6.978 ± 0.081
5.188LeuGlu: 5.188 ± 0.081
3.693LeuPhe: 3.693 ± 0.066
8.289LeuGly: 8.289 ± 0.095
2.38LeuHis: 2.38 ± 0.051
5.103LeuIle: 5.103 ± 0.075
3.133LeuLys: 3.133 ± 0.063
11.777LeuLeu: 11.777 ± 0.155
2.598LeuMet: 2.598 ± 0.051
2.801LeuAsn: 2.801 ± 0.072
6.277LeuPro: 6.277 ± 0.086
3.343LeuGln: 3.343 ± 0.061
8.365LeuArg: 8.365 ± 0.101
6.615LeuSer: 6.615 ± 0.096
5.812LeuThr: 5.812 ± 0.09
7.335LeuVal: 7.335 ± 0.09
1.308LeuTrp: 1.308 ± 0.04
2.2LeuTyr: 2.2 ± 0.048
0.0LeuXaa: 0.0 ± 0.0
Met
2.705MetAla: 2.705 ± 0.054
0.162MetCys: 0.162 ± 0.013
1.24MetAsp: 1.24 ± 0.036
1.019MetGlu: 1.019 ± 0.033
0.751MetPhe: 0.751 ± 0.028
1.583MetGly: 1.583 ± 0.042
0.569MetHis: 0.569 ± 0.022
1.004MetIle: 1.004 ± 0.028
1.068MetLys: 1.068 ± 0.029
2.676MetLeu: 2.676 ± 0.056
0.616MetMet: 0.616 ± 0.025
0.932MetAsn: 0.932 ± 0.027
1.397MetPro: 1.397 ± 0.032
1.061MetGln: 1.061 ± 0.03
1.894MetArg: 1.894 ± 0.038
1.77MetSer: 1.77 ± 0.044
1.568MetThr: 1.568 ± 0.035
1.62MetVal: 1.62 ± 0.038
0.223MetTrp: 0.223 ± 0.013
0.402MetTyr: 0.402 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
3.085AsnAla: 3.085 ± 0.082
0.276AsnCys: 0.276 ± 0.017
1.402AsnAsp: 1.402 ± 0.037
1.229AsnGlu: 1.229 ± 0.033
0.894AsnPhe: 0.894 ± 0.027
2.225AsnGly: 2.225 ± 0.104
0.501AsnHis: 0.501 ± 0.023
1.218AsnIle: 1.218 ± 0.044
0.684AsnLys: 0.684 ± 0.028
2.607AsnLeu: 2.607 ± 0.06
0.55AsnMet: 0.55 ± 0.018
0.744AsnAsn: 0.744 ± 0.049
1.68AsnPro: 1.68 ± 0.046
0.724AsnGln: 0.724 ± 0.026
1.804AsnArg: 1.804 ± 0.042
1.22AsnSer: 1.22 ± 0.059
1.441AsnThr: 1.441 ± 0.059
1.913AsnVal: 1.913 ± 0.045
0.41AsnTrp: 0.41 ± 0.02
0.593AsnTyr: 0.593 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
6.801ProAla: 6.801 ± 0.106
0.372ProCys: 0.372 ± 0.021
3.824ProAsp: 3.824 ± 0.066
3.212ProGlu: 3.212 ± 0.062
1.701ProPhe: 1.701 ± 0.035
4.501ProGly: 4.501 ± 0.074
1.054ProHis: 1.054 ± 0.031
1.803ProIle: 1.803 ± 0.044
1.249ProLys: 1.249 ± 0.044
5.187ProLeu: 5.187 ± 0.083
1.171ProMet: 1.171 ± 0.033
1.088ProAsn: 1.088 ± 0.032
2.716ProPro: 2.716 ± 0.072
1.783ProGln: 1.783 ± 0.039
3.001ProArg: 3.001 ± 0.058
2.434ProSer: 2.434 ± 0.047
2.235ProThr: 2.235 ± 0.047
4.447ProVal: 4.447 ± 0.071
0.731ProTrp: 0.731 ± 0.021
1.018ProTyr: 1.018 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
4.492GlnAla: 4.492 ± 0.067
0.3GlnCys: 0.3 ± 0.018
1.529GlnAsp: 1.529 ± 0.041
1.279GlnGlu: 1.279 ± 0.039
1.141GlnPhe: 1.141 ± 0.032
2.492GlnGly: 2.492 ± 0.047
0.817GlnHis: 0.817 ± 0.026
1.802GlnIle: 1.802 ± 0.037
0.931GlnLys: 0.931 ± 0.03
3.675GlnLeu: 3.675 ± 0.06
0.891GlnMet: 0.891 ± 0.032
0.709GlnAsn: 0.709 ± 0.021
2.067GlnPro: 2.067 ± 0.053
1.537GlnGln: 1.537 ± 0.04
3.047GlnArg: 3.047 ± 0.059
1.842GlnSer: 1.842 ± 0.043
1.83GlnThr: 1.83 ± 0.043
2.603GlnVal: 2.603 ± 0.045
0.493GlnTrp: 0.493 ± 0.023
0.721GlnTyr: 0.721 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
8.835ArgAla: 8.835 ± 0.114
0.693ArgCys: 0.693 ± 0.029
4.523ArgAsp: 4.523 ± 0.067
4.506ArgGlu: 4.506 ± 0.076
2.879ArgPhe: 2.879 ± 0.051
4.895ArgGly: 4.895 ± 0.083
2.01ArgHis: 2.01 ± 0.055
4.133ArgIle: 4.133 ± 0.062
1.951ArgLys: 1.951 ± 0.051
8.568ArgLeu: 8.568 ± 0.11
2.107ArgMet: 2.107 ± 0.046
1.969ArgAsn: 1.969 ± 0.035
3.332ArgPro: 3.332 ± 0.066
2.567ArgGln: 2.567 ± 0.062
5.815ArgArg: 5.815 ± 0.095
4.065ArgSer: 4.065 ± 0.061
3.557ArgThr: 3.557 ± 0.06
5.667ArgVal: 5.667 ± 0.08
1.148ArgTrp: 1.148 ± 0.034
1.998ArgTyr: 1.998 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
7.155SerAla: 7.155 ± 0.093
0.513SerCys: 0.513 ± 0.023
3.015SerAsp: 3.015 ± 0.063
2.668SerGlu: 2.668 ± 0.049
2.096SerPhe: 2.096 ± 0.051
5.874SerGly: 5.874 ± 0.189
1.218SerHis: 1.218 ± 0.032
2.51SerIle: 2.51 ± 0.056
1.365SerLys: 1.365 ± 0.039
5.914SerLeu: 5.914 ± 0.095
1.391SerMet: 1.391 ± 0.033
1.407SerAsn: 1.407 ± 0.049
2.763SerPro: 2.763 ± 0.047
1.696SerGln: 1.696 ± 0.036
4.101SerArg: 4.101 ± 0.063
3.239SerSer: 3.239 ± 0.086
2.845SerThr: 2.845 ± 0.066
4.248SerVal: 4.248 ± 0.064
0.725SerTrp: 0.725 ± 0.026
1.114SerTyr: 1.114 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
5.87ThrAla: 5.87 ± 0.093
0.442ThrCys: 0.442 ± 0.022
2.875ThrAsp: 2.875 ± 0.053
2.503ThrGlu: 2.503 ± 0.044
1.781ThrPhe: 1.781 ± 0.054
4.911ThrGly: 4.911 ± 0.131
1.177ThrHis: 1.177 ± 0.03
2.032ThrIle: 2.032 ± 0.055
1.055ThrLys: 1.055 ± 0.033
6.834ThrLeu: 6.834 ± 0.148
0.982ThrMet: 0.982 ± 0.033
1.074ThrAsn: 1.074 ± 0.052
3.259ThrPro: 3.259 ± 0.059
1.766ThrGln: 1.766 ± 0.045
3.759ThrArg: 3.759 ± 0.052
2.541ThrSer: 2.541 ± 0.053
2.648ThrThr: 2.648 ± 0.064
4.109ThrVal: 4.109 ± 0.064
0.637ThrTrp: 0.637 ± 0.025
0.999ThrTyr: 0.999 ± 0.031
0.0ThrXaa: 0.0 ± 0.0
Val
9.205ValAla: 9.205 ± 0.1
0.795ValCys: 0.795 ± 0.024
4.283ValAsp: 4.283 ± 0.069
4.17ValGlu: 4.17 ± 0.058
2.867ValPhe: 2.867 ± 0.058
5.386ValGly: 5.386 ± 0.072
1.404ValHis: 1.404 ± 0.034
3.687ValIle: 3.687 ± 0.057
2.284ValLys: 2.284 ± 0.058
8.251ValLeu: 8.251 ± 0.101
1.967ValMet: 1.967 ± 0.047
1.881ValAsn: 1.881 ± 0.053
3.682ValPro: 3.682 ± 0.056
2.386ValGln: 2.386 ± 0.051
5.567ValArg: 5.567 ± 0.079
4.842ValSer: 4.842 ± 0.069
4.251ValThr: 4.251 ± 0.077
6.107ValVal: 6.107 ± 0.087
0.936ValTrp: 0.936 ± 0.032
1.328ValTyr: 1.328 ± 0.038
0.0ValXaa: 0.0 ± 0.0
Trp
1.266TrpAla: 1.266 ± 0.036
0.155TrpCys: 0.155 ± 0.011
0.582TrpAsp: 0.582 ± 0.024
0.517TrpGlu: 0.517 ± 0.026
0.524TrpPhe: 0.524 ± 0.021
0.825TrpGly: 0.825 ± 0.028
0.366TrpHis: 0.366 ± 0.02
0.633TrpIle: 0.633 ± 0.022
0.421TrpLys: 0.421 ± 0.022
2.069TrpLeu: 2.069 ± 0.051
0.386TrpMet: 0.386 ± 0.018
0.381TrpAsn: 0.381 ± 0.015
0.677TrpPro: 0.677 ± 0.027
0.675TrpGln: 0.675 ± 0.028
1.186TrpArg: 1.186 ± 0.035
0.822TrpSer: 0.822 ± 0.027
0.741TrpThr: 0.741 ± 0.03
1.007TrpVal: 1.007 ± 0.031
0.241TrpTrp: 0.241 ± 0.015
0.293TrpTyr: 0.293 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.476TyrAla: 2.476 ± 0.05
0.267TyrCys: 0.267 ± 0.016
1.228TyrAsp: 1.228 ± 0.037
1.083TyrGlu: 1.083 ± 0.029
0.905TyrPhe: 0.905 ± 0.027
1.859TyrGly: 1.859 ± 0.038
0.411TyrHis: 0.411 ± 0.017
0.888TyrIle: 0.888 ± 0.028
0.537TyrLys: 0.537 ± 0.022
2.279TyrLeu: 2.279 ± 0.042
0.451TyrMet: 0.451 ± 0.023
0.588TyrAsn: 0.588 ± 0.028
1.03TyrPro: 1.03 ± 0.033
0.73TyrGln: 0.73 ± 0.023
1.92TyrArg: 1.92 ± 0.042
1.242TyrSer: 1.242 ± 0.038
1.078TyrThr: 1.078 ± 0.034
1.572TyrVal: 1.572 ± 0.036
0.326TyrTrp: 0.326 ± 0.019
0.549TyrTyr: 0.549 ± 0.024
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3549 proteins (1184287 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski