Marinicauda sp. WD6-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Maricaulales; Maricaulaceae; Marinicauda

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2648 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2U2BR03|A0A2U2BR03_9PROT Uncharacterized protein OS=Marinicauda sp. WD6-1 OX=2135793 GN=DDZ18_13600 PE=4 SV=1
MM1 pKa = 7.33SVFATTLGLVAIGAGASGLAAQEE24 pKa = 3.81EE25 pKa = 4.35DD26 pKa = 3.81ADD28 pKa = 4.06AKK30 pKa = 10.5IASAMSAAPAVISADD45 pKa = 3.51ATIMDD50 pKa = 4.13HH51 pKa = 7.41DD52 pKa = 4.13GTVLRR57 pKa = 11.84EE58 pKa = 4.01GDD60 pKa = 3.79NGWTCFPEE68 pKa = 4.39TAALGAMCNEE78 pKa = 3.99AHH80 pKa = 6.07WNEE83 pKa = 4.24MIGALMAQEE92 pKa = 5.35DD93 pKa = 4.53FTASEE98 pKa = 4.34FSVSYY103 pKa = 10.08MLAGDD108 pKa = 4.28GAAPGVSNIDD118 pKa = 3.52PYY120 pKa = 10.78ATEE123 pKa = 4.0PTEE126 pKa = 4.72DD127 pKa = 3.71NDD129 pKa = 3.74WVRR132 pKa = 11.84EE133 pKa = 4.36GPHH136 pKa = 7.77LMIIVPDD143 pKa = 3.94PAMLEE148 pKa = 4.2GMSTDD153 pKa = 3.51PADD156 pKa = 3.71PVYY159 pKa = 11.3VMWKK163 pKa = 6.95DD164 pKa = 3.31TPYY167 pKa = 9.51AHH169 pKa = 6.96IMVRR173 pKa = 11.84VEE175 pKa = 3.84AYY177 pKa = 8.18EE178 pKa = 3.74

Molecular weight:
18.79 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2U2BY07|A0A2U2BY07_9PROT Uncharacterized protein OS=Marinicauda sp. WD6-1 OX=2135793 GN=DDZ18_04695 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.84LVRR12 pKa = 11.84KK13 pKa = 8.95RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.63GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.2AGRR28 pKa = 11.84RR29 pKa = 11.84VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84SKK37 pKa = 10.49GRR39 pKa = 11.84KK40 pKa = 8.72RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight:
5.28 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2648

0

2648

855958

41

3096

323.2

34.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.554 ± 0.082

0.746 ± 0.014

6.672 ± 0.057

7.205 ± 0.051

3.646 ± 0.03

8.937 ± 0.049

1.781 ± 0.02

4.362 ± 0.028

2.417 ± 0.049

9.497 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.19 ± 0.025

2.097 ± 0.026

5.257 ± 0.033

2.428 ± 0.023

7.937 ± 0.058

4.756 ± 0.034

4.752 ± 0.037

7.312 ± 0.036

1.36 ± 0.021

2.096 ± 0.025

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski