Marinicauda sp. WD6-1
Average proteome isoelectric point is 5.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2648 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U2BR03|A0A2U2BR03_9PROT Uncharacterized protein OS=Marinicauda sp. WD6-1 OX=2135793 GN=DDZ18_13600 PE=4 SV=1
MM1 pKa = 7.33 SVFATTLGLVAIGAGASGLAAQEE24 pKa = 3.81 EE25 pKa = 4.35 DD26 pKa = 3.81 ADD28 pKa = 4.06 AKK30 pKa = 10.5 IASAMSAAPAVISADD45 pKa = 3.51 ATIMDD50 pKa = 4.13 HH51 pKa = 7.41 DD52 pKa = 4.13 GTVLRR57 pKa = 11.84 EE58 pKa = 4.01 GDD60 pKa = 3.79 NGWTCFPEE68 pKa = 4.39 TAALGAMCNEE78 pKa = 3.99 AHH80 pKa = 6.07 WNEE83 pKa = 4.24 MIGALMAQEE92 pKa = 5.35 DD93 pKa = 4.53 FTASEE98 pKa = 4.34 FSVSYY103 pKa = 10.08 MLAGDD108 pKa = 4.28 GAAPGVSNIDD118 pKa = 3.52 PYY120 pKa = 10.78 ATEE123 pKa = 4.0 PTEE126 pKa = 4.72 DD127 pKa = 3.71 NDD129 pKa = 3.74 WVRR132 pKa = 11.84 EE133 pKa = 4.36 GPHH136 pKa = 7.77 LMIIVPDD143 pKa = 3.94 PAMLEE148 pKa = 4.2 GMSTDD153 pKa = 3.51 PADD156 pKa = 3.71 PVYY159 pKa = 11.3 VMWKK163 pKa = 6.95 DD164 pKa = 3.31 TPYY167 pKa = 9.51 AHH169 pKa = 6.96 IMVRR173 pKa = 11.84 VEE175 pKa = 3.84 AYY177 pKa = 8.18 EE178 pKa = 3.74
Molecular weight: 18.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.783
IPC_protein 3.757
Toseland 3.554
ProMoST 3.884
Dawson 3.745
Bjellqvist 3.935
Wikipedia 3.668
Rodwell 3.592
Grimsley 3.465
Solomon 3.732
Lehninger 3.681
Nozaki 3.859
DTASelect 4.062
Thurlkill 3.605
EMBOSS 3.681
Sillero 3.884
Patrickios 0.985
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.796
Protein with the highest isoelectric point:
>tr|A0A2U2BY07|A0A2U2BY07_9PROT Uncharacterized protein OS=Marinicauda sp. WD6-1 OX=2135793 GN=DDZ18_04695 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.84 LVRR12 pKa = 11.84 KK13 pKa = 8.95 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.63 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.2 AGRR28 pKa = 11.84 RR29 pKa = 11.84 VIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.49 GRR39 pKa = 11.84 KK40 pKa = 8.72 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.33
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.749
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.486
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.236
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2648
0
2648
855958
41
3096
323.2
34.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.554 ± 0.082
0.746 ± 0.014
6.672 ± 0.057
7.205 ± 0.051
3.646 ± 0.03
8.937 ± 0.049
1.781 ± 0.02
4.362 ± 0.028
2.417 ± 0.049
9.497 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.19 ± 0.025
2.097 ± 0.026
5.257 ± 0.033
2.428 ± 0.023
7.937 ± 0.058
4.756 ± 0.034
4.752 ± 0.037
7.312 ± 0.036
1.36 ± 0.021
2.096 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here