Fusarium sp. AF-8

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium; Fusarium solani species complex; unclassified Fusarium solani species complex

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16262 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A428PLE0|A0A428PLE0_9HYPO UBX domain-containing protein OS=Fusarium sp. AF-8 OX=1325734 GN=CEP54_010223 PE=4 SV=1
MM1 pKa = 7.2STTTTTTAAATATTACTNLYY21 pKa = 10.44DD22 pKa = 5.33SPNQDD27 pKa = 3.35TTCAMPFTGNHH38 pKa = 4.66TDD40 pKa = 4.29IMKK43 pKa = 10.26ACCGDD48 pKa = 3.6AQIVSYY54 pKa = 11.18HH55 pKa = 6.22NDD57 pKa = 3.14CGIYY61 pKa = 10.19CVALDD66 pKa = 3.68QTVSDD71 pKa = 5.38LADD74 pKa = 3.39CLYY77 pKa = 11.26KK78 pKa = 10.87NGAPWEE84 pKa = 4.44SVFCSVPKK92 pKa = 10.52SVSSDD97 pKa = 3.33VASKK101 pKa = 9.77TKK103 pKa = 10.54DD104 pKa = 3.2AAVPASAQASVISGGDD120 pKa = 3.51DD121 pKa = 3.84NKK123 pKa = 11.45DD124 pKa = 3.41DD125 pKa = 5.91DD126 pKa = 4.93KK127 pKa = 12.02DD128 pKa = 3.72SDD130 pKa = 4.27KK131 pKa = 11.66DD132 pKa = 4.04SDD134 pKa = 5.29DD135 pKa = 4.31KK136 pKa = 11.89DD137 pKa = 4.71SDD139 pKa = 4.59DD140 pKa = 4.58SDD142 pKa = 3.77STASGTASSASSTSTGNAAAGVVPQSSVSTLGLTIGALLFSSMALGAFNII192 pKa = 4.38

Molecular weight: 19.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A428PG36|A0A428PG36_9HYPO WHIM1 domain-containing protein OS=Fusarium sp. AF-8 OX=1325734 GN=CEP54_011126 PE=4 SV=1
MM1 pKa = 7.86PLTRR5 pKa = 11.84THH7 pKa = 6.67RR8 pKa = 11.84HH9 pKa = 3.83TTPRR13 pKa = 11.84RR14 pKa = 11.84SIFSTRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84APARR26 pKa = 11.84SHH28 pKa = 5.86HH29 pKa = 6.11TVTTTTTTTRR39 pKa = 11.84KK40 pKa = 8.96PRR42 pKa = 11.84RR43 pKa = 11.84GLLGGGRR50 pKa = 11.84RR51 pKa = 11.84THH53 pKa = 7.04AAPVHH58 pKa = 5.1HH59 pKa = 5.69QQRR62 pKa = 11.84RR63 pKa = 11.84PSMKK67 pKa = 10.24DD68 pKa = 2.95KK69 pKa = 11.41VSGALLKK76 pKa = 11.04LKK78 pKa = 10.68GSLTRR83 pKa = 11.84RR84 pKa = 11.84PGVKK88 pKa = 9.89AAGTRR93 pKa = 11.84RR94 pKa = 11.84MRR96 pKa = 11.84GTDD99 pKa = 2.74GRR101 pKa = 11.84GSHH104 pKa = 5.81RR105 pKa = 11.84RR106 pKa = 11.84ARR108 pKa = 11.84HH109 pKa = 4.06FF110 pKa = 3.77

Molecular weight: 12.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16262

0

16262

7482626

50

8440

460.1

51.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.455 ± 0.019

1.319 ± 0.008

5.907 ± 0.015

6.316 ± 0.02

3.815 ± 0.011

6.886 ± 0.019

2.376 ± 0.01

4.962 ± 0.012

4.858 ± 0.015

8.94 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.008

3.592 ± 0.009

6.013 ± 0.024

3.935 ± 0.015

5.981 ± 0.016

7.933 ± 0.023

5.94 ± 0.019

6.193 ± 0.014

1.602 ± 0.007

2.738 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski