Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Haloarculaceae; Haloarcula; Haloarcula marismortui

Average proteome isoelectric point is 4.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4234 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5UWZ5|Q5UWZ5_HALMA Uncharacterized protein OS=Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) OX=272569 GN=rrnAC3542 PE=4 SV=1
MM1 pKa = 7.57SEE3 pKa = 3.97YY4 pKa = 10.66TGDD7 pKa = 3.25VTLNGGIEE15 pKa = 4.34TPVRR19 pKa = 11.84MSGLEE24 pKa = 3.92DD25 pKa = 3.91MYY27 pKa = 11.26VQPDD31 pKa = 4.38SIDD34 pKa = 3.32GDD36 pKa = 3.77LDD38 pKa = 3.74LRR40 pKa = 11.84NVEE43 pKa = 4.52YY44 pKa = 10.9VFTGVPITPAADD56 pKa = 3.22VADD59 pKa = 4.38PVTDD63 pKa = 3.21ITGTLEE69 pKa = 4.65DD70 pKa = 5.67GYY72 pKa = 9.36TEE74 pKa = 4.42PDD76 pKa = 3.77GVHH79 pKa = 6.79GDD81 pKa = 3.84LAVGTAEE88 pKa = 5.07DD89 pKa = 3.69VFIAHH94 pKa = 6.96GAVDD98 pKa = 4.05GALSARR104 pKa = 11.84GPEE107 pKa = 3.96QVFDD111 pKa = 4.73AGTSDD116 pKa = 3.77APSRR120 pKa = 11.84DD121 pKa = 3.18PATYY125 pKa = 10.6DD126 pKa = 3.22VTVSGWQQQRR136 pKa = 11.84EE137 pKa = 4.35VTDD140 pKa = 4.06PNTGVVLSGCQSTVNVTGATGSVSCYY166 pKa = 10.71LIGTNNEE173 pKa = 4.11LIVRR177 pKa = 11.84GDD179 pKa = 3.36AAVEE183 pKa = 3.64VHH185 pKa = 6.68IVGRR189 pKa = 11.84DD190 pKa = 3.23NRR192 pKa = 11.84VDD194 pKa = 3.89LGPYY198 pKa = 9.43IDD200 pKa = 4.53VDD202 pKa = 3.94TAVEE206 pKa = 4.18TGFDD210 pKa = 3.5NTIDD214 pKa = 3.9VQPFPADD221 pKa = 3.56DD222 pKa = 4.88LIEE225 pKa = 4.3TTEE228 pKa = 4.2DD229 pKa = 3.08EE230 pKa = 4.79AYY232 pKa = 10.62SAVTFGRR239 pKa = 11.84AKK241 pKa = 9.3VTYY244 pKa = 9.95QSVAEE249 pKa = 4.39DD250 pKa = 3.93EE251 pKa = 4.42DD252 pKa = 3.66WCRR255 pKa = 11.84GCGEE259 pKa = 4.09AADD262 pKa = 5.06AIIEE266 pKa = 4.19RR267 pKa = 11.84QQKK270 pKa = 9.1EE271 pKa = 3.87AFFILGTPIITYY283 pKa = 9.89EE284 pKa = 4.08EE285 pKa = 4.62GGGSYY290 pKa = 10.06EE291 pKa = 4.73CEE293 pKa = 4.22HH294 pKa = 6.98CAHH297 pKa = 6.94SAPDD301 pKa = 3.49TSLTPEE307 pKa = 3.71EE308 pKa = 4.06RR309 pKa = 11.84RR310 pKa = 11.84EE311 pKa = 3.99IFF313 pKa = 3.63

Molecular weight: 33.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5V814|Q5V814_HALMA MCM / cell division control protein 21 OS=Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) OX=272569 GN=mcm3 PE=3 SV=1
MM1 pKa = 7.23QNTHH5 pKa = 7.18AIEE8 pKa = 3.98QKK10 pKa = 10.44GRR12 pKa = 11.84GIAVALRR19 pKa = 11.84PSPRR23 pKa = 11.84DD24 pKa = 3.17AGAGSGRR31 pKa = 11.84ASAGASVQPVPAPGALPAGHH51 pKa = 6.89PRR53 pKa = 11.84SHH55 pKa = 6.26EE56 pKa = 3.85VRR58 pKa = 11.84HH59 pKa = 5.27RR60 pKa = 11.84ASRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84TRR67 pKa = 11.84SAPAASFRR75 pKa = 11.84LRR77 pKa = 11.84RR78 pKa = 11.84SGPSPGAPGRR88 pKa = 11.84HH89 pKa = 6.15AGLAGGSRR97 pKa = 11.84PYY99 pKa = 10.53SEE101 pKa = 5.45RR102 pKa = 11.84SYY104 pKa = 11.68SCIQPPRR111 pKa = 11.84EE112 pKa = 4.09IVDD115 pKa = 3.32EE116 pKa = 4.2RR117 pKa = 11.84APDD120 pKa = 3.21VWANGPAAFVLRR132 pKa = 11.84ALAPPCEE139 pKa = 4.24CFIGRR144 pKa = 11.84VCEE147 pKa = 4.02FDD149 pKa = 3.94LLGAVVASDD158 pKa = 4.14DD159 pKa = 3.67GHH161 pKa = 7.24GEE163 pKa = 4.0LVRR166 pKa = 11.84VPVVGRR172 pKa = 11.84AFAVDD177 pKa = 4.07AQEE180 pKa = 3.25QWAALRR186 pKa = 11.84EE187 pKa = 4.24QFTGNTFLRR196 pKa = 11.84MCVSRR201 pKa = 11.84ADD203 pKa = 3.71RR204 pKa = 11.84FTSRR208 pKa = 11.84RR209 pKa = 11.84SRR211 pKa = 11.84LTHH214 pKa = 5.8GSQWNRR220 pKa = 11.84RR221 pKa = 11.84AWCSSTRR228 pKa = 11.84RR229 pKa = 11.84DD230 pKa = 3.33SRR232 pKa = 11.84SRR234 pKa = 11.84KK235 pKa = 9.62HH236 pKa = 6.27EE237 pKa = 3.53IAQRR241 pKa = 11.84RR242 pKa = 11.84VCSYY246 pKa = 10.13QKK248 pKa = 10.53HH249 pKa = 5.5SAPTTIKK256 pKa = 10.52PFQRR260 pKa = 11.84WNNILLNEE268 pKa = 4.08LQEE271 pKa = 4.28GAEE274 pKa = 4.11YY275 pKa = 10.84EE276 pKa = 4.01RR277 pKa = 11.84TNRR280 pKa = 11.84KK281 pKa = 9.03RR282 pKa = 11.84SPSIRR287 pKa = 11.84PRR289 pKa = 11.84EE290 pKa = 4.01QSPVRR295 pKa = 11.84HH296 pKa = 5.69NQRR299 pKa = 11.84CSGRR303 pKa = 11.84VLISKK308 pKa = 8.67QANHH312 pKa = 5.75QKK314 pKa = 9.21ATEE317 pKa = 4.21QPCSEE322 pKa = 3.94RR323 pKa = 11.84SAVQKK328 pKa = 10.78RR329 pKa = 11.84GWCKK333 pKa = 10.16LSSLARR339 pKa = 11.84PSLGFCIHH347 pKa = 6.2QGPLAFVTKK356 pKa = 9.89PVYY359 pKa = 10.29FGCTNSPHH367 pKa = 5.07VQQTILYY374 pKa = 8.66KK375 pKa = 10.69LVEE378 pKa = 4.41TVSGLFLTHH387 pKa = 6.93LNFVHH392 pKa = 7.96DD393 pKa = 4.59IGCSRR398 pKa = 11.84KK399 pKa = 10.19SFWPNSNLPKK409 pKa = 10.14KK410 pKa = 9.73YY411 pKa = 10.22NIVISRR417 pKa = 11.84FWARR421 pKa = 11.84HH422 pKa = 3.93CWQFYY427 pKa = 9.29SPCSKK432 pKa = 10.76AILVPFSSKK441 pKa = 10.6FGIVPALEE449 pKa = 4.14LLGGSLFSRR458 pKa = 11.84YY459 pKa = 7.96MRR461 pKa = 11.84DD462 pKa = 3.12LPGSNRR468 pKa = 11.84LLKK471 pKa = 10.46LSHH474 pKa = 6.74RR475 pKa = 11.84GWGPADD481 pKa = 3.27PVAYY485 pKa = 10.13RR486 pKa = 11.84IMTEE490 pKa = 3.74TTRR493 pKa = 11.84PQNDD497 pKa = 3.43TAQDD501 pKa = 3.36RR502 pKa = 11.84QEE504 pKa = 4.77RR505 pKa = 11.84RR506 pKa = 11.84LQTAKK511 pKa = 10.88RR512 pKa = 11.84LLTEE516 pKa = 4.75ADD518 pKa = 2.79TDD520 pKa = 3.83AAVIEE525 pKa = 4.57DD526 pKa = 4.28CQTVLSRR533 pKa = 11.84PGYY536 pKa = 9.53EE537 pKa = 3.94RR538 pKa = 11.84SHH540 pKa = 6.03SQASLDD546 pKa = 3.57ACSALLALAQGDD558 pKa = 4.02EE559 pKa = 4.36STKK562 pKa = 9.56RR563 pKa = 11.84TRR565 pKa = 11.84EE566 pKa = 3.89TKK568 pKa = 9.96QVLTAVSRR576 pKa = 11.84RR577 pKa = 11.84GGDD580 pKa = 3.36CC581 pKa = 3.77

Molecular weight: 64.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4234

0

4234

1200597

30

2306

283.6

30.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.415 ± 0.044

0.757 ± 0.012

8.326 ± 0.043

8.09 ± 0.051

3.254 ± 0.027

8.278 ± 0.035

2.005 ± 0.017

4.386 ± 0.029

2.001 ± 0.021

8.81 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.878 ± 0.017

2.586 ± 0.025

4.591 ± 0.027

3.124 ± 0.023

6.114 ± 0.034

5.955 ± 0.033

6.916 ± 0.037

8.648 ± 0.035

1.147 ± 0.016

2.718 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski