Diaphorina citri (Asian citrus psyllid)
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 21517 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S3DPW0|A0A1S3DPW0_DIACI transmembrane protein 179-like OS=Diaphorina citri OX=121845 GN=LOC103521862 PE=4 SV=2
MM1 pKa = 7.79 DD2 pKa = 6.18 LDD4 pKa = 4.15 GDD6 pKa = 4.97 GYY8 pKa = 11.28 LSMYY12 pKa = 8.98 EE13 pKa = 4.08 LEE15 pKa = 4.29 YY16 pKa = 10.53 FYY18 pKa = 11.03 DD19 pKa = 3.84 EE20 pKa = 3.91 QLQRR24 pKa = 11.84 MDD26 pKa = 4.95 AIGIEE31 pKa = 4.27 CLPFEE36 pKa = 5.55 DD37 pKa = 5.43 CLCQMLDD44 pKa = 3.48 MIKK47 pKa = 10.46 PEE49 pKa = 4.21 VPGKK53 pKa = 10.49 ISLQDD58 pKa = 3.5 LKK60 pKa = 10.36 RR61 pKa = 11.84 CKK63 pKa = 7.72 MTRR66 pKa = 11.84 IFFDD70 pKa = 3.23 TFFNLEE76 pKa = 4.08 KK77 pKa = 11.05 YY78 pKa = 10.31 LDD80 pKa = 3.81 HH81 pKa = 6.62 EE82 pKa = 4.32 QRR84 pKa = 11.84 DD85 pKa = 4.0 PFASQRR91 pKa = 11.84 DD92 pKa = 4.13 DD93 pKa = 3.91 DD94 pKa = 5.6 SEE96 pKa = 4.19 MSDD99 pKa = 2.64 WDD101 pKa = 3.61 RR102 pKa = 11.84 FASEE106 pKa = 4.65 EE107 pKa = 3.96 YY108 pKa = 11.15 DD109 pKa = 3.23 MLVVEE114 pKa = 5.41 EE115 pKa = 5.1 GGNDD119 pKa = 3.49 NQDD122 pKa = 3.1 DD123 pKa = 4.38 MMYY126 pKa = 9.81 MDD128 pKa = 5.37 QEE130 pKa = 4.7 PDD132 pKa = 3.5 LLSPNLDD139 pKa = 3.48 QILYY143 pKa = 9.61 WNRR146 pKa = 11.84 QQTSHH151 pKa = 6.64 IKK153 pKa = 9.84 SAPGINGIEE162 pKa = 4.25 YY163 pKa = 9.64 EE164 pKa = 4.19 GGMGGGSLSSDD175 pKa = 3.65 DD176 pKa = 4.01 SGDD179 pKa = 3.79 YY180 pKa = 11.04 ADD182 pKa = 5.92 SDD184 pKa = 4.73 DD185 pKa = 4.91 DD186 pKa = 4.64 CSLL189 pKa = 3.61
Molecular weight: 21.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.685
IPC2_protein 3.694
IPC_protein 3.719
Toseland 3.49
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.668
Rodwell 3.541
Grimsley 3.401
Solomon 3.706
Lehninger 3.668
Nozaki 3.834
DTASelect 4.088
Thurlkill 3.554
EMBOSS 3.668
Sillero 3.846
Patrickios 1.189
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.758
Protein with the highest isoelectric point:
>tr|A0A3Q0IXE5|A0A3Q0IXE5_DIACI 26S proteasome regulatory subunit 4-like OS=Diaphorina citri OX=121845 GN=LOC113468296 PE=4 SV=1
MM1 pKa = 6.31 VTTRR5 pKa = 11.84 RR6 pKa = 11.84 KK7 pKa = 9.74 RR8 pKa = 11.84 EE9 pKa = 3.56 KK10 pKa = 10.02 NRR12 pKa = 11.84 NIRR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 NLKK20 pKa = 9.54 ARR22 pKa = 11.84 KK23 pKa = 8.16 QKK25 pKa = 10.39 KK26 pKa = 9.45 HH27 pKa = 5.01 STTITKK33 pKa = 9.85 KK34 pKa = 8.05 RR35 pKa = 11.84 TPRR38 pKa = 11.84 RR39 pKa = 11.84 VTIRR43 pKa = 11.84 KK44 pKa = 9.38 RR45 pKa = 11.84 NIRR48 pKa = 11.84 RR49 pKa = 11.84 NTTTPRR55 pKa = 11.84 EE56 pKa = 3.94 RR57 pKa = 11.84 RR58 pKa = 11.84 RR59 pKa = 11.84 SIRR62 pKa = 11.84 WRR64 pKa = 11.84 RR65 pKa = 11.84 SITIRR70 pKa = 11.84 NMRR73 pKa = 11.84 QKK75 pKa = 10.51 RR76 pKa = 11.84 EE77 pKa = 3.8 RR78 pKa = 11.84 RR79 pKa = 11.84 EE80 pKa = 3.88 PSLKK84 pKa = 10.86 SLDD87 pKa = 3.56 LTRR90 pKa = 11.84 KK91 pKa = 6.9 VTVRR95 pKa = 11.84 RR96 pKa = 11.84 VNTTFTRR103 pKa = 3.72
Molecular weight: 12.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.407
IPC2_protein 10.862
IPC_protein 12.457
Toseland 12.632
ProMoST 13.13
Dawson 12.632
Bjellqvist 12.618
Wikipedia 13.1
Rodwell 12.296
Grimsley 12.661
Solomon 13.13
Lehninger 13.027
Nozaki 12.632
DTASelect 12.618
Thurlkill 12.632
EMBOSS 13.13
Sillero 12.632
Patrickios 12.003
IPC_peptide 13.13
IPC2_peptide 12.12
IPC2.peptide.svr19 9.105
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19805
1712
21517
8003745
34
10264
372.0
41.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.412 ± 0.016
1.984 ± 0.022
5.485 ± 0.015
6.652 ± 0.024
3.737 ± 0.013
5.267 ± 0.022
2.638 ± 0.013
5.53 ± 0.019
7.033 ± 0.026
8.957 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.277 ± 0.012
5.496 ± 0.019
5.348 ± 0.032
4.17 ± 0.019
4.983 ± 0.017
8.767 ± 0.03
5.977 ± 0.02
6.034 ± 0.016
0.978 ± 0.006
3.269 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here