Campylobacter avium LMG 24591
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1636 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A222MUV8|A0A222MUV8_9PROT Tryptophan--tRNA ligase OS=Campylobacter avium LMG 24591 OX=522484 GN=trpS PE=3 SV=1
MM1 pKa = 7.24 SVAVIYY7 pKa = 10.24 GSSMGNTEE15 pKa = 3.78 GAANLIAEE23 pKa = 4.6 KK24 pKa = 10.75 LGVSDD29 pKa = 4.3 VLNISDD35 pKa = 4.32 IDD37 pKa = 3.57 AAKK40 pKa = 10.32 INSYY44 pKa = 11.18 DD45 pKa = 3.6 KK46 pKa = 11.19 LIVGSSTWGSGDD58 pKa = 4.97 LQDD61 pKa = 5.35 DD62 pKa = 3.26 WDD64 pKa = 4.37 AFDD67 pKa = 5.53 FSGLKK72 pKa = 10.3 LDD74 pKa = 4.26 GKK76 pKa = 8.21 TVAVFGMGDD85 pKa = 3.54 SEE87 pKa = 5.1 SYY89 pKa = 11.34 SDD91 pKa = 3.87 TYY93 pKa = 10.59 CNAMGKK99 pKa = 9.56 LAEE102 pKa = 4.41 KK103 pKa = 10.24 LQAAGAKK110 pKa = 9.96 LVGQVSTDD118 pKa = 3.19 GYY120 pKa = 10.55 SYY122 pKa = 10.64 EE123 pKa = 4.12 SSEE126 pKa = 4.06 AVVDD130 pKa = 4.43 GKK132 pKa = 11.02 FVGLALDD139 pKa = 4.09 NDD141 pKa = 3.86 NQEE144 pKa = 4.59 DD145 pKa = 3.92 LTEE148 pKa = 3.96 EE149 pKa = 4.75 RR150 pKa = 11.84 ISNWVEE156 pKa = 3.56 QIKK159 pKa = 11.03 ADD161 pKa = 4.02 FNN163 pKa = 4.17
Molecular weight: 17.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.177
IPC2_protein 3.872
IPC_protein 3.859
Toseland 3.643
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.795
Rodwell 3.681
Grimsley 3.554
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.215
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.986
Patrickios 3.516
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.917
Protein with the highest isoelectric point:
>tr|A0A222MZH3|A0A222MZH3_9PROT Exonuclease V helicase AddB OS=Campylobacter avium LMG 24591 OX=522484 GN=addB PE=4 SV=1
MM1 pKa = 7.59 AKK3 pKa = 10.37 KK4 pKa = 10.94 SMIAKK9 pKa = 9.61 AARR12 pKa = 11.84 KK13 pKa = 9.51 AKK15 pKa = 10.14 FSVRR19 pKa = 11.84 AYY21 pKa = 8.5 TRR23 pKa = 11.84 CQICGRR29 pKa = 11.84 PHH31 pKa = 5.56 SVYY34 pKa = 10.37 RR35 pKa = 11.84 DD36 pKa = 3.43 FGICRR41 pKa = 11.84 VCLRR45 pKa = 11.84 KK46 pKa = 8.66 MANEE50 pKa = 3.81 GLIPGLKK57 pKa = 8.97 KK58 pKa = 10.89 ASWW61 pKa = 3.28
Molecular weight: 6.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.78
IPC_protein 10.233
Toseland 10.833
ProMoST 10.409
Dawson 10.906
Bjellqvist 10.57
Wikipedia 11.067
Rodwell 11.33
Grimsley 10.935
Solomon 10.994
Lehninger 10.979
Nozaki 10.833
DTASelect 10.555
Thurlkill 10.818
EMBOSS 11.228
Sillero 10.833
Patrickios 11.096
IPC_peptide 11.008
IPC2_peptide 9.706
IPC2.peptide.svr19 8.528
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1636
0
1636
522044
37
2497
319.1
36.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.972 ± 0.055
1.202 ± 0.026
5.732 ± 0.045
6.906 ± 0.066
6.022 ± 0.064
5.14 ± 0.061
1.539 ± 0.021
7.964 ± 0.054
9.751 ± 0.077
11.209 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.103 ± 0.026
5.854 ± 0.055
2.484 ± 0.031
2.897 ± 0.032
3.224 ± 0.033
7.297 ± 0.046
3.879 ± 0.044
5.389 ± 0.045
0.568 ± 0.016
3.867 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here