Lujinxingia litoralis
Average proteome isoelectric point is 5.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3776 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A328CBD2|A0A328CBD2_9DELT Uncharacterized protein OS=Lujinxingia litoralis OX=2211119 GN=DL240_09360 PE=4 SV=1
MM1 pKa = 7.56 ALSACGGDD9 pKa = 3.88 PEE11 pKa = 4.97 PDD13 pKa = 3.25 RR14 pKa = 11.84 NPDD17 pKa = 2.86 VGQDD21 pKa = 3.4 VDD23 pKa = 4.34 PDD25 pKa = 3.95 PDD27 pKa = 5.43 AGDD30 pKa = 3.63 TDD32 pKa = 3.89 VDD34 pKa = 3.69 PDD36 pKa = 4.25 ADD38 pKa = 3.83 VDD40 pKa = 3.89 PDD42 pKa = 4.15 ADD44 pKa = 3.71 VDD46 pKa = 3.98 PDD48 pKa = 3.76 TDD50 pKa = 3.97 VDD52 pKa = 3.6 ITDD55 pKa = 4.11 PPEE58 pKa = 3.95 DD59 pKa = 5.42 AIACDD64 pKa = 4.08 EE65 pKa = 4.82 PMPQPPQGEE74 pKa = 4.3 RR75 pKa = 11.84 CVVIPGNGDD84 pKa = 3.68 HH85 pKa = 6.95 ILFRR89 pKa = 11.84 GTLLAGDD96 pKa = 4.3 DD97 pKa = 4.25 VYY99 pKa = 11.45 HH100 pKa = 6.81 EE101 pKa = 5.02 GSLLLNDD108 pKa = 3.78 QSPNRR113 pKa = 11.84 QIVCSGCGCADD124 pKa = 3.42 TPEE127 pKa = 4.56 AQDD130 pKa = 3.57 ATIVSCPSGVISPGLINPHH149 pKa = 5.7 DD150 pKa = 5.38 HH151 pKa = 5.8 ITYY154 pKa = 10.02 SLSHH158 pKa = 6.54 PRR160 pKa = 11.84 PHH162 pKa = 7.58 GEE164 pKa = 3.44 EE165 pKa = 4.32 RR166 pKa = 11.84 FDD168 pKa = 4.93 HH169 pKa = 5.93 RR170 pKa = 11.84 HH171 pKa = 4.86 DD172 pKa = 3.04 WRR174 pKa = 11.84 RR175 pKa = 11.84 GLRR178 pKa = 11.84 GHH180 pKa = 6.48 DD181 pKa = 4.25 QINTSPGSDD190 pKa = 3.19 NSHH193 pKa = 6.26 EE194 pKa = 4.67 GILYY198 pKa = 10.71 GEE200 pKa = 4.29 LRR202 pKa = 11.84 MLFGGATSVVGSVGSGDD219 pKa = 3.37 ASGMLRR225 pKa = 11.84 NLDD228 pKa = 3.4 NTSYY232 pKa = 11.02 TEE234 pKa = 4.05 GLSGVDD240 pKa = 2.75 VSYY243 pKa = 9.28 RR244 pKa = 11.84 TFPLGDD250 pKa = 3.47 SNGTLRR256 pKa = 11.84 ASGCDD261 pKa = 3.45 YY262 pKa = 11.26 PNIDD266 pKa = 3.51 NEE268 pKa = 4.23 SRR270 pKa = 11.84 LNSGVYY276 pKa = 9.92 LPHH279 pKa = 8.02 LSEE282 pKa = 6.05 GIDD285 pKa = 3.23 PEE287 pKa = 4.45 ANNEE291 pKa = 3.91 FHH293 pKa = 6.87 CASGASGSDD302 pKa = 3.92 LIQDD306 pKa = 3.61 NTSIIHH312 pKa = 6.9 GIGLSTRR319 pKa = 11.84 DD320 pKa = 3.25 IALMARR326 pKa = 11.84 RR327 pKa = 11.84 GATLVWSARR336 pKa = 11.84 TNIDD340 pKa = 3.45 LYY342 pKa = 11.39 GNTAQAPIFKK352 pKa = 10.38 RR353 pKa = 11.84 FGVPIALGTDD363 pKa = 3.55 WSASGSMNMLRR374 pKa = 11.84 EE375 pKa = 4.21 LQCADD380 pKa = 3.42 YY381 pKa = 11.18 LNRR384 pKa = 11.84 LYY386 pKa = 10.99 YY387 pKa = 10.64 DD388 pKa = 3.3 EE389 pKa = 5.29 TFTEE393 pKa = 4.15 QEE395 pKa = 3.9 LWMMATANAADD406 pKa = 3.63 AMGAGDD412 pKa = 3.52 QIGRR416 pKa = 11.84 LEE418 pKa = 4.11 EE419 pKa = 5.08 GYY421 pKa = 11.14 VGDD424 pKa = 3.48 ITIFDD429 pKa = 3.77 GTDD432 pKa = 2.97 RR433 pKa = 11.84 LPYY436 pKa = 9.9 RR437 pKa = 11.84 AIIDD441 pKa = 3.9 AEE443 pKa = 3.92 IADD446 pKa = 3.72 IVLVLRR452 pKa = 11.84 GGEE455 pKa = 4.14 PLYY458 pKa = 11.38 GDD460 pKa = 3.72 AQLIEE465 pKa = 4.47 ALVDD469 pKa = 3.62 SAEE472 pKa = 4.61 LDD474 pKa = 3.45 GCEE477 pKa = 4.66 QIDD480 pKa = 3.58 VCEE483 pKa = 4.16 RR484 pKa = 11.84 GRR486 pKa = 11.84 RR487 pKa = 11.84 LCVEE491 pKa = 4.28 LDD493 pKa = 3.18 AGKK496 pKa = 10.13 SLSAIRR502 pKa = 11.84 SAVSSNAYY510 pKa = 9.65 EE511 pKa = 4.44 LFFCGEE517 pKa = 4.1 PDD519 pKa = 4.97 DD520 pKa = 5.52 EE521 pKa = 4.7 PSCMPFRR528 pKa = 11.84 PNEE531 pKa = 4.0 YY532 pKa = 10.41 SGLTDD537 pKa = 3.34 NTDD540 pKa = 2.99 NSGDD544 pKa = 4.16 GIPDD548 pKa = 3.51 AVDD551 pKa = 3.02 NCPAYY556 pKa = 10.42 FNPIRR561 pKa = 11.84 PMDD564 pKa = 4.33 GGQQPDD570 pKa = 3.61 TNGNGIGDD578 pKa = 3.74 ICDD581 pKa = 3.91 PCPLSEE587 pKa = 5.77 DD588 pKa = 4.32 PNCNTIDD595 pKa = 4.49 PDD597 pKa = 4.22 DD598 pKa = 5.08 LDD600 pKa = 5.86 GDD602 pKa = 4.38 GVANDD607 pKa = 3.83 TDD609 pKa = 3.53 NCPVHH614 pKa = 6.71 FNPGQEE620 pKa = 4.13 NTSGDD625 pKa = 3.99 AYY627 pKa = 10.89 GDD629 pKa = 3.59 ACSPCPEE636 pKa = 4.18 TFLGEE641 pKa = 4.5 GEE643 pKa = 4.32 ACPVSIYY650 pKa = 10.32 SIKK653 pKa = 11.01 NGTTDD658 pKa = 3.72 PGSLGTFEE666 pKa = 4.79 GVIVTAVAEE675 pKa = 4.43 GEE677 pKa = 4.55 GFFVQVDD684 pKa = 4.06 PQSDD688 pKa = 4.12 DD689 pKa = 3.62 YY690 pKa = 11.9 QGDD693 pKa = 3.95 QYY695 pKa = 11.86 SGIYY699 pKa = 9.32 VYY701 pKa = 11.07 NRR703 pKa = 11.84 GGTVFPQVGDD713 pKa = 4.15 RR714 pKa = 11.84 IDD716 pKa = 3.59 LTGSSTLFYY725 pKa = 11.08 GQFQVGNVSAINILEE740 pKa = 4.14 SGYY743 pKa = 9.54 PLPAPTVVSPAEE755 pKa = 3.99 VANNGALRR763 pKa = 11.84 QAYY766 pKa = 9.16 EE767 pKa = 4.18 GVLVRR772 pKa = 11.84 VEE774 pKa = 4.65 DD775 pKa = 3.61 VTVTNNSPDD784 pKa = 3.9 PGPGQGDD791 pKa = 3.64 NPFEE795 pKa = 4.1 FAVDD799 pKa = 3.01 SGLRR803 pKa = 11.84 INNLMYY809 pKa = 10.52 TIDD812 pKa = 4.21 PKK814 pKa = 11.26 PEE816 pKa = 3.87 VGNSFASITGVLRR829 pKa = 11.84 WANEE833 pKa = 3.73 NSKK836 pKa = 10.37 VEE838 pKa = 4.11 PRR840 pKa = 11.84 SEE842 pKa = 4.33 LDD844 pKa = 3.62 VVSGPPFLAAASPEE858 pKa = 3.96 ALFIDD863 pKa = 4.4 ADD865 pKa = 4.01 GADD868 pKa = 3.93 GQLTLSLNRR877 pKa = 11.84 ASQGEE882 pKa = 4.43 STLALSYY889 pKa = 10.83 NPAGIISGPTSATLADD905 pKa = 4.34 GEE907 pKa = 4.39 QSVTVAIAATTPDD920 pKa = 3.49 AEE922 pKa = 4.36 ATISVTLDD930 pKa = 3.01 GVTLTIPVTTYY941 pKa = 10.25 SAASPRR947 pKa = 11.84 EE948 pKa = 3.99 LSSLSASADD957 pKa = 3.7 TIFVGDD963 pKa = 3.62 QVNFDD968 pKa = 4.59 LEE970 pKa = 4.62 LNLPAGAAGEE980 pKa = 4.42 TVSLNLLPVEE990 pKa = 4.38 TTLPFPAEE998 pKa = 3.84 VSFAAGEE1005 pKa = 3.86 QRR1007 pKa = 11.84 ANITLTFNEE1016 pKa = 4.58 GAGDD1020 pKa = 3.81 YY1021 pKa = 9.95 TLEE1024 pKa = 4.31 ATLGTTTLSADD1035 pKa = 3.52 VTVANAPDD1043 pKa = 3.65 EE1044 pKa = 4.11 QSEE1047 pKa = 4.61 SFINFDD1053 pKa = 4.44 GPGNTYY1059 pKa = 10.61 GAGSFVGDD1067 pKa = 3.05 SGYY1070 pKa = 9.27 TFNYY1074 pKa = 8.0 TGGRR1078 pKa = 11.84 LVDD1081 pKa = 3.49 EE1082 pKa = 5.11 TSNSDD1087 pKa = 3.27 YY1088 pKa = 11.05 TIDD1091 pKa = 4.12 GRR1093 pKa = 11.84 GLMFGGSGDD1102 pKa = 3.26 KK1103 pKa = 10.92 SLIVQGLEE1111 pKa = 3.74 GGINSLRR1118 pKa = 11.84 LEE1120 pKa = 3.86 MRR1122 pKa = 11.84 KK1123 pKa = 9.9 AFTSGANRR1131 pKa = 11.84 QIEE1134 pKa = 4.46 VFVNGTSVGTSEE1146 pKa = 4.55 VFGNASGADD1155 pKa = 3.59 ATVHH1159 pKa = 6.16 EE1160 pKa = 5.19 LLLEE1164 pKa = 5.0 DD1165 pKa = 4.25 INISGTFDD1173 pKa = 3.57 LEE1175 pKa = 3.81 IRR1177 pKa = 11.84 SIQSGQVTIDD1187 pKa = 3.36 NLVWGSFLPP1196 pKa = 4.17
Molecular weight: 126.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.701
IPC2_protein 3.795
IPC_protein 3.834
Toseland 3.605
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.745
Rodwell 3.656
Grimsley 3.516
Solomon 3.821
Lehninger 3.783
Nozaki 3.923
DTASelect 4.177
Thurlkill 3.656
EMBOSS 3.757
Sillero 3.948
Patrickios 1.659
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.854
Protein with the highest isoelectric point:
>tr|A0A328C748|A0A328C748_9DELT Peptidase_M23 domain-containing protein OS=Lujinxingia litoralis OX=2211119 GN=DL240_09425 PE=4 SV=1
MM1 pKa = 7.74 RR2 pKa = 11.84 SGRR5 pKa = 11.84 RR6 pKa = 11.84 VRR8 pKa = 11.84 GRR10 pKa = 11.84 GRR12 pKa = 11.84 GRR14 pKa = 11.84 ARR16 pKa = 11.84 VRR18 pKa = 11.84 GRR20 pKa = 11.84 VRR22 pKa = 11.84 GRR24 pKa = 11.84 GRR26 pKa = 11.84 GRR28 pKa = 11.84 GRR30 pKa = 11.84 VRR32 pKa = 11.84 ARR34 pKa = 11.84 ARR36 pKa = 11.84 ARR38 pKa = 11.84 ARR40 pKa = 11.84 GRR42 pKa = 11.84 VRR44 pKa = 11.84 VRR46 pKa = 11.84 GRR48 pKa = 11.84 GRR50 pKa = 11.84 GRR52 pKa = 11.84 GRR54 pKa = 11.84 GRR56 pKa = 11.84 GRR58 pKa = 11.84 GRR60 pKa = 11.84 VRR62 pKa = 11.84 GRR64 pKa = 11.84 VRR66 pKa = 11.84 ARR68 pKa = 11.84 RR69 pKa = 11.84 KK70 pKa = 7.79 TPHH73 pKa = 6.15 LQRR76 pKa = 11.84 LPGVGCFF83 pKa = 3.82
Molecular weight: 9.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.51
IPC2_protein 11.462
IPC_protein 13.071
Toseland 13.232
ProMoST 13.729
Dawson 13.232
Bjellqvist 13.232
Wikipedia 13.715
Rodwell 12.749
Grimsley 13.276
Solomon 13.729
Lehninger 13.627
Nozaki 13.232
DTASelect 13.232
Thurlkill 13.232
EMBOSS 13.729
Sillero 13.232
Patrickios 12.457
IPC_peptide 13.729
IPC2_peptide 12.72
IPC2.peptide.svr19 9.359
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3776
0
3776
1447238
37
4109
383.3
41.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.323 ± 0.058
1.131 ± 0.034
5.85 ± 0.038
7.575 ± 0.049
3.445 ± 0.024
8.221 ± 0.043
2.303 ± 0.021
4.119 ± 0.029
2.24 ± 0.026
10.653 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.111 ± 0.017
2.377 ± 0.025
5.441 ± 0.037
3.746 ± 0.025
7.703 ± 0.047
5.71 ± 0.029
5.047 ± 0.034
7.343 ± 0.038
1.313 ± 0.017
2.348 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here