Yersinia ruckeri
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3467 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A085U820|A0A085U820_YERRU ATP-binding transport protein OS=Yersinia ruckeri OX=29486 GN=CSF007_9895 PE=4 SV=1
MM1 pKa = 7.75 ISDD4 pKa = 4.49 KK5 pKa = 11.37 YY6 pKa = 11.27 GDD8 pKa = 4.24 GPDD11 pKa = 3.74 PYY13 pKa = 9.72 TYY15 pKa = 10.43 PNSQVLINKK24 pKa = 9.35 FDD26 pKa = 3.58 ITDD29 pKa = 3.73 DD30 pKa = 3.79 SQFVEE35 pKa = 4.43 MEE37 pKa = 3.74 QDD39 pKa = 3.5 FSEE42 pKa = 4.24 LAIMDD47 pKa = 4.23 IEE49 pKa = 4.55 FSPPPYY55 pKa = 10.48 DD56 pKa = 3.58 LLYY59 pKa = 10.21 WRR61 pKa = 11.84 SLHH64 pKa = 6.64
Molecular weight: 7.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.745
IPC_protein 3.681
Toseland 3.465
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.389
Solomon 3.668
Lehninger 3.617
Nozaki 3.834
DTASelect 4.062
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.808
Patrickios 0.693
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.742
Protein with the highest isoelectric point:
>tr|A0A085U8X5|A0A085U8X5_YERRU Uncharacterized protein OS=Yersinia ruckeri OX=29486 GN=CSF007_11120 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.35 NGRR28 pKa = 11.84 LVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.34 SRR39 pKa = 11.84 TRR41 pKa = 11.84 LTVSKK46 pKa = 10.95
Molecular weight: 5.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3467
0
3467
1084460
29
3980
312.8
34.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.188 ± 0.055
1.061 ± 0.014
5.177 ± 0.032
5.547 ± 0.041
3.826 ± 0.031
7.102 ± 0.042
2.27 ± 0.018
6.274 ± 0.037
4.496 ± 0.043
11.055 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.696 ± 0.021
4.047 ± 0.036
4.354 ± 0.029
4.823 ± 0.04
5.464 ± 0.038
6.236 ± 0.035
5.321 ± 0.039
6.815 ± 0.043
1.347 ± 0.018
2.9 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here