Ostreococcus tauri
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7745 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A090N3I6|A0A090N3I6_OSTTA Unnamed product OS=Ostreococcus tauri OX=70448 GN=OT_ostta06g00010 PE=4 SV=1
MM1 pKa = 7.73 SSEE4 pKa = 4.17 EE5 pKa = 3.99 QLSEE9 pKa = 4.1 ALADD13 pKa = 3.43 AARR16 pKa = 11.84 YY17 pKa = 9.68 GEE19 pKa = 4.16 YY20 pKa = 10.75 DD21 pKa = 3.36 EE22 pKa = 5.61 ARR24 pKa = 11.84 AYY26 pKa = 10.94 LDD28 pKa = 3.16 QGALADD34 pKa = 4.66 AYY36 pKa = 10.96 DD37 pKa = 4.01 GLLYY41 pKa = 10.7 AAANGHH47 pKa = 5.41 EE48 pKa = 4.5 EE49 pKa = 4.15 IVNLLLEE56 pKa = 4.28 YY57 pKa = 10.68 GADD60 pKa = 3.42 VNKK63 pKa = 10.23 TNEE66 pKa = 4.0 QGSTALHH73 pKa = 5.87 WACLNGHH80 pKa = 6.69 PGIVQLLLDD89 pKa = 4.36 KK90 pKa = 11.11 GATPKK95 pKa = 10.04 ICNEE99 pKa = 4.08 AGRR102 pKa = 11.84 TALDD106 pKa = 3.49 EE107 pKa = 4.47 AMHH110 pKa = 6.36 NARR113 pKa = 11.84 DD114 pKa = 3.74 EE115 pKa = 4.28 CVKK118 pKa = 10.56 ILMEE122 pKa = 4.51 AEE124 pKa = 4.09 GEE126 pKa = 3.97 EE127 pKa = 4.28 DD128 pKa = 4.26 VEE130 pKa = 4.74 LEE132 pKa = 4.7 EE133 pKa = 5.71 IDD135 pKa = 4.66 EE136 pKa = 4.36 EE137 pKa = 4.32 DD138 pKa = 4.58 AEE140 pKa = 5.25 DD141 pKa = 3.62 VTTDD145 pKa = 3.54 EE146 pKa = 5.09 EE147 pKa = 4.48 EE148 pKa = 4.21 QGMDD152 pKa = 3.05
Molecular weight: 16.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.859
IPC_protein 3.808
Toseland 3.63
ProMoST 3.91
Dawson 3.77
Bjellqvist 3.961
Wikipedia 3.656
Rodwell 3.643
Grimsley 3.541
Solomon 3.77
Lehninger 3.719
Nozaki 3.884
DTASelect 4.037
Thurlkill 3.656
EMBOSS 3.681
Sillero 3.923
Patrickios 1.85
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.846
Protein with the highest isoelectric point:
>tr|A0A090N3E1|A0A090N3E1_OSTTA Inosine/uridine-preferring nucleoside hydrolase OS=Ostreococcus tauri OX=70448 GN=OT_ostta05g02130 PE=3 SV=1
MM1 pKa = 7.89 PSNKK5 pKa = 8.27 TFKK8 pKa = 10.16 IKK10 pKa = 10.51 KK11 pKa = 9.52 ILGKK15 pKa = 9.62 KK16 pKa = 8.76 QKK18 pKa = 9.06 QNRR21 pKa = 11.84 PLPQWIRR28 pKa = 11.84 MRR30 pKa = 11.84 TGNTIRR36 pKa = 11.84 YY37 pKa = 5.79 NAKK40 pKa = 8.47 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.11 WRR45 pKa = 11.84 RR46 pKa = 11.84 TKK48 pKa = 11.06 LNLL51 pKa = 3.44
Molecular weight: 6.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.366
IPC2_protein 11.023
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.384
Grimsley 12.544
Solomon 12.983
Lehninger 12.896
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.106
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 8.974
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7743
2
7745
3561553
30
11265
459.9
50.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.475 ± 0.042
1.612 ± 0.014
6.337 ± 0.02
7.185 ± 0.036
3.447 ± 0.017
7.275 ± 0.032
1.932 ± 0.014
3.89 ± 0.019
4.698 ± 0.026
8.181 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.439 ± 0.012
2.933 ± 0.016
4.232 ± 0.027
2.582 ± 0.019
7.969 ± 0.034
7.155 ± 0.028
5.773 ± 0.02
7.422 ± 0.022
1.218 ± 0.009
2.228 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here