Amino acid dipepetide frequency for Ostreococcus tauri

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.452AlaAla: 12.452 ± 0.119	2.124AlaCys: 2.124 ± 0.028	5.267AlaAsp: 5.267 ± 0.04	7.077AlaGlu: 7.077 ± 0.072	3.998AlaPhe: 3.998 ± 0.046	6.333AlaGly: 6.333 ± 0.051	2.374AlaHis: 2.374 ± 0.032	4.291AlaIle: 4.291 ± 0.04	5.597AlaLys: 5.597 ± 0.061	11.522AlaLeu: 11.522 ± 0.081	3.219AlaMet: 3.219 ± 0.026	3.413AlaAsn: 3.413 ± 0.032	4.9AlaPro: 4.9 ± 0.045	3.001AlaGln: 3.001 ± 0.038	11.504AlaArg: 11.504 ± 0.1	9.535AlaSer: 9.535 ± 0.066	6.812AlaThr: 6.812 ± 0.05	7.473AlaVal: 7.473 ± 0.051	1.396AlaTrp: 1.396 ± 0.027	2.452AlaTyr: 2.452 ± 0.031	0.013AlaXaa: 0.013 ± 0.002
Cys	2.37CysAla: 2.37 ± 0.033	0.286CysCys: 0.286 ± 0.011	1.141CysAsp: 1.141 ± 0.02	1.254CysGlu: 1.254 ± 0.022	0.546CysPhe: 0.546 ± 0.014	1.402CysGly: 1.402 ± 0.024	0.288CysHis: 0.288 ± 0.009	0.533CysIle: 0.533 ± 0.013	0.58CysLys: 0.58 ± 0.015	1.142CysLeu: 1.142 ± 0.022	0.312CysMet: 0.312 ± 0.01	0.358CysAsn: 0.358 ± 0.012	0.653CysPro: 0.653 ± 0.021	0.349CysGln: 0.349 ± 0.011	0.915CysArg: 0.915 ± 0.02	0.982CysSer: 0.982 ± 0.023	0.784CysThr: 0.784 ± 0.018	1.679CysVal: 1.679 ± 0.028	0.193CysTrp: 0.193 ± 0.008	0.352CysTyr: 0.352 ± 0.011	0.001CysXaa: 0.001 ± 0.0
Asp	9.59AspAla: 9.59 ± 0.071	0.735AspCys: 0.735 ± 0.017	6.525AspAsp: 6.525 ± 0.085	5.869AspGlu: 5.869 ± 0.053	2.186AspPhe: 2.186 ± 0.029	5.959AspGly: 5.959 ± 0.045	0.865AspHis: 0.865 ± 0.017	2.331AspIle: 2.331 ± 0.028	2.067AspLys: 2.067 ± 0.026	4.355AspLeu: 4.355 ± 0.036	1.236AspMet: 1.236 ± 0.02	1.212AspAsn: 1.212 ± 0.019	2.404AspPro: 2.404 ± 0.026	1.054AspGln: 1.054 ± 0.022	3.253AspArg: 3.253 ± 0.032	3.226AspSer: 3.226 ± 0.037	2.27AspThr: 2.27 ± 0.029	6.227AspVal: 6.227 ± 0.052	0.743AspTrp: 0.743 ± 0.016	1.265AspTyr: 1.265 ± 0.02	0.003AspXaa: 0.003 ± 0.001
Glu	7.533GluAla: 7.533 ± 0.066	1.089GluCys: 1.089 ± 0.021	4.778GluAsp: 4.778 ± 0.047	5.88GluGlu: 5.88 ± 0.071	2.228GluPhe: 2.228 ± 0.028	4.186GluGly: 4.186 ± 0.043	1.461GluHis: 1.461 ± 0.023	3.195GluIle: 3.195 ± 0.031	3.707GluLys: 3.707 ± 0.048	6.355GluLeu: 6.355 ± 0.07	1.769GluMet: 1.769 ± 0.025	2.364GluAsn: 2.364 ± 0.03	2.25GluPro: 2.25 ± 0.027	2.002GluGln: 2.002 ± 0.03	7.299GluArg: 7.299 ± 0.068	4.652GluSer: 4.652 ± 0.046	4.378GluThr: 4.378 ± 0.039	4.288GluVal: 4.288 ± 0.038	0.82GluTrp: 0.82 ± 0.017	1.609GluTyr: 1.609 ± 0.022	0.004GluXaa: 0.004 ± 0.001
Phe	3.865PheAla: 3.865 ± 0.038	0.562PheCys: 0.562 ± 0.013	2.59PheAsp: 2.59 ± 0.027	2.535PheGlu: 2.535 ± 0.028	1.292PhePhe: 1.292 ± 0.023	2.957PheGly: 2.957 ± 0.044	0.704PheHis: 0.704 ± 0.016	1.463PheIle: 1.463 ± 0.023	1.47PheLys: 1.47 ± 0.02	2.622PheLeu: 2.622 ± 0.031	0.812PheMet: 0.812 ± 0.016	1.166PheAsn: 1.166 ± 0.029	1.335PhePro: 1.335 ± 0.02	0.879PheGln: 0.879 ± 0.018	1.844PheArg: 1.844 ± 0.027	2.237PheSer: 2.237 ± 0.034	1.969PheThr: 1.969 ± 0.027	2.882PheVal: 2.882 ± 0.035	0.426PheTrp: 0.426 ± 0.013	0.857PheTyr: 0.857 ± 0.017	0.005PheXaa: 0.005 ± 0.001
Gly	8.269GlyAla: 8.269 ± 0.06	1.147GlyCys: 1.147 ± 0.022	4.975GlyAsp: 4.975 ± 0.04	5.128GlyGlu: 5.128 ± 0.05	2.622GlyPhe: 2.622 ± 0.029	7.121GlyGly: 7.121 ± 0.08	1.255GlyHis: 1.255 ± 0.026	2.657GlyIle: 2.657 ± 0.035	3.468GlyLys: 3.468 ± 0.035	5.191GlyLeu: 5.191 ± 0.045	1.751GlyMet: 1.751 ± 0.024	1.908GlyAsn: 1.908 ± 0.025	2.155GlyPro: 2.155 ± 0.027	1.593GlyGln: 1.593 ± 0.024	5.548GlyArg: 5.548 ± 0.057	5.002GlySer: 5.002 ± 0.048	4.32GlyThr: 4.32 ± 0.047	5.951GlyVal: 5.951 ± 0.057	0.99GlyTrp: 0.99 ± 0.019	1.696GlyTyr: 1.696 ± 0.024	0.004GlyXaa: 0.004 ± 0.001
His	2.485HisAla: 2.485 ± 0.028	0.325HisCys: 0.325 ± 0.01	1.083HisAsp: 1.083 ± 0.019	1.299HisGlu: 1.299 ± 0.02	0.7HisPhe: 0.7 ± 0.014	1.658HisGly: 1.658 ± 0.025	0.491HisHis: 0.491 ± 0.016	0.739HisIle: 0.739 ± 0.016	0.716HisLys: 0.716 ± 0.014	1.555HisLeu: 1.555 ± 0.021	0.42HisMet: 0.42 ± 0.012	0.476HisAsn: 0.476 ± 0.013	0.991HisPro: 0.991 ± 0.017	0.49HisGln: 0.49 ± 0.011	1.419HisArg: 1.419 ± 0.023	1.118HisSer: 1.118 ± 0.022	0.932HisThr: 0.932 ± 0.02	1.706HisVal: 1.706 ± 0.022	0.26HisTrp: 0.26 ± 0.008	0.454HisTyr: 0.454 ± 0.012	0.001HisXaa: 0.001 ± 0.001
Ile	4.584IleAla: 4.584 ± 0.044	0.564IleCys: 0.564 ± 0.013	2.963IleAsp: 2.963 ± 0.03	3.252IleGlu: 3.252 ± 0.036	1.366IlePhe: 1.366 ± 0.024	2.934IleGly: 2.934 ± 0.029	0.732IleHis: 0.732 ± 0.016	1.59IleIle: 1.59 ± 0.025	1.705IleLys: 1.705 ± 0.025	3.097IleLeu: 3.097 ± 0.043	0.855IleMet: 0.855 ± 0.017	1.117IleAsn: 1.117 ± 0.02	1.772IlePro: 1.772 ± 0.022	1.078IleGln: 1.078 ± 0.018	2.435IleArg: 2.435 ± 0.029	2.531IleSer: 2.531 ± 0.033	1.966IleThr: 1.966 ± 0.025	3.226IleVal: 3.226 ± 0.029	0.404IleTrp: 0.404 ± 0.012	0.729IleTyr: 0.729 ± 0.015	0.003IleXaa: 0.003 ± 0.001
Lys	4.579LysAla: 4.579 ± 0.054	0.753LysCys: 0.753 ± 0.017	2.339LysAsp: 2.339 ± 0.031	3.153LysGlu: 3.153 ± 0.042	1.611LysPhe: 1.611 ± 0.025	2.645LysGly: 2.645 ± 0.035	0.98LysHis: 0.98 ± 0.018	2.055LysIle: 2.055 ± 0.031	3.31LysLys: 3.31 ± 0.054	4.036LysLeu: 4.036 ± 0.038	1.32LysMet: 1.32 ± 0.02	1.513LysAsn: 1.513 ± 0.021	2.03LysPro: 2.03 ± 0.03	1.398LysGln: 1.398 ± 0.023	4.038LysArg: 4.038 ± 0.044	3.41LysSer: 3.41 ± 0.038	3.1LysThr: 3.1 ± 0.033	2.876LysVal: 2.876 ± 0.032	0.574LysTrp: 0.574 ± 0.014	1.253LysTyr: 1.253 ± 0.021	0.007LysXaa: 0.007 ± 0.001
Leu	9.067LeuAla: 9.067 ± 0.066	1.419LeuCys: 1.419 ± 0.022	5.17LeuAsp: 5.17 ± 0.038	6.259LeuGlu: 6.259 ± 0.072	2.665LeuPhe: 2.665 ± 0.031	5.92LeuGly: 5.92 ± 0.045	1.719LeuHis: 1.719 ± 0.027	3.085LeuIle: 3.085 ± 0.032	4.141LeuLys: 4.141 ± 0.049	6.614LeuLeu: 6.614 ± 0.066	1.912LeuMet: 1.912 ± 0.023	2.651LeuAsn: 2.651 ± 0.037	3.532LeuPro: 3.532 ± 0.039	2.46LeuGln: 2.46 ± 0.035	6.783LeuArg: 6.783 ± 0.056	5.747LeuSer: 5.747 ± 0.052	4.506LeuThr: 4.506 ± 0.038	5.516LeuVal: 5.516 ± 0.043	0.884LeuTrp: 0.884 ± 0.02	1.751LeuTyr: 1.751 ± 0.025	0.008LeuXaa: 0.008 ± 0.002
Met	2.257MetAla: 2.257 ± 0.028	0.453MetCys: 0.453 ± 0.012	1.459MetAsp: 1.459 ± 0.022	1.563MetGlu: 1.563 ± 0.022	0.966MetPhe: 0.966 ± 0.03	1.499MetGly: 1.499 ± 0.027	0.504MetHis: 0.504 ± 0.013	0.98MetIle: 0.98 ± 0.019	1.299MetLys: 1.299 ± 0.023	1.99MetLeu: 1.99 ± 0.024	0.727MetMet: 0.727 ± 0.015	0.868MetAsn: 0.868 ± 0.016	1.079MetPro: 1.079 ± 0.018	0.738MetGln: 0.738 ± 0.016	2.15MetArg: 2.15 ± 0.027	2.067MetSer: 2.067 ± 0.022	1.569MetThr: 1.569 ± 0.021	1.325MetVal: 1.325 ± 0.02	0.262MetTrp: 0.262 ± 0.009	0.637MetTyr: 0.637 ± 0.014	0.002MetXaa: 0.002 ± 0.001
Asn	4.085AsnAla: 4.085 ± 0.036	0.391AsnCys: 0.391 ± 0.011	1.971AsnAsp: 1.971 ± 0.027	2.138AsnGlu: 2.138 ± 0.029	1.074AsnPhe: 1.074 ± 0.019	2.41AsnGly: 2.41 ± 0.026	0.521AsnHis: 0.521 ± 0.012	1.247AsnIle: 1.247 ± 0.02	1.274AsnLys: 1.274 ± 0.022	2.226AsnLeu: 2.226 ± 0.031	0.689AsnMet: 0.689 ± 0.016	0.774AsnAsn: 0.774 ± 0.021	1.47AsnPro: 1.47 ± 0.022	0.732AsnGln: 0.732 ± 0.024	1.588AsnArg: 1.588 ± 0.022	1.691AsnSer: 1.691 ± 0.025	1.434AsnThr: 1.434 ± 0.031	2.603AsnVal: 2.603 ± 0.027	0.358AsnTrp: 0.358 ± 0.01	0.646AsnTyr: 0.646 ± 0.017	0.003AsnXaa: 0.003 ± 0.001
Pro	3.803ProAla: 3.803 ± 0.047	0.539ProCys: 0.539 ± 0.013	2.127ProAsp: 2.127 ± 0.023	2.938ProGlu: 2.938 ± 0.034	1.45ProPhe: 1.45 ± 0.027	2.956ProGly: 2.956 ± 0.034	0.82ProHis: 0.82 ± 0.014	1.627ProIle: 1.627 ± 0.022	2.108ProLys: 2.108 ± 0.028	3.191ProLeu: 3.191 ± 0.031	1.027ProMet: 1.027 ± 0.019	1.434ProAsn: 1.434 ± 0.023	3.191ProPro: 3.191 ± 0.088	1.082ProGln: 1.082 ± 0.021	3.15ProArg: 3.15 ± 0.032	3.822ProSer: 3.822 ± 0.042	2.823ProThr: 2.823 ± 0.038	2.756ProVal: 2.756 ± 0.03	0.552ProTrp: 0.552 ± 0.013	0.915ProTyr: 0.915 ± 0.017	0.005ProXaa: 0.005 ± 0.001
Gln	2.545GlnAla: 2.545 ± 0.04	0.454GlnCys: 0.454 ± 0.013	1.205GlnAsp: 1.205 ± 0.023	1.534GlnGlu: 1.534 ± 0.024	0.879GlnPhe: 0.879 ± 0.018	1.602GlnGly: 1.602 ± 0.023	0.553GlnHis: 0.553 ± 0.013	1.229GlnIle: 1.229 ± 0.019	1.383GlnLys: 1.383 ± 0.023	2.446GlnLeu: 2.446 ± 0.041	0.719GlnMet: 0.719 ± 0.016	0.868GlnAsn: 0.868 ± 0.018	1.074GlnPro: 1.074 ± 0.022	1.031GlnGln: 1.031 ± 0.026	2.247GlnArg: 2.247 ± 0.028	1.839GlnSer: 1.839 ± 0.025	1.534GlnThr: 1.534 ± 0.025	1.653GlnVal: 1.653 ± 0.025	0.338GlnTrp: 0.338 ± 0.01	0.683GlnTyr: 0.683 ± 0.014	0.003GlnXaa: 0.003 ± 0.001
Arg	12.11ArgAla: 12.11 ± 0.095	1.246ArgCys: 1.246 ± 0.02	5.051ArgAsp: 5.051 ± 0.045	6.478ArgGlu: 6.478 ± 0.059	2.282ArgPhe: 2.282 ± 0.026	6.218ArgGly: 6.218 ± 0.053	1.334ArgHis: 1.334 ± 0.023	2.438ArgIle: 2.438 ± 0.029	3.168ArgLys: 3.168 ± 0.038	5.832ArgLeu: 5.832 ± 0.044	1.745ArgMet: 1.745 ± 0.023	1.862ArgAsn: 1.862 ± 0.023	2.614ArgPro: 2.614 ± 0.032	1.89ArgGln: 1.89 ± 0.028	7.64ArgArg: 7.64 ± 0.072	4.984ArgSer: 4.984 ± 0.044	4.036ArgThr: 4.036 ± 0.036	6.167ArgVal: 6.167 ± 0.047	1.094ArgTrp: 1.094 ± 0.019	1.494ArgTyr: 1.494 ± 0.02	0.008ArgXaa: 0.008 ± 0.001
Ser	8.292SerAla: 8.292 ± 0.064	1.015SerCys: 1.015 ± 0.023	4.266SerAsp: 4.266 ± 0.041	4.748SerGlu: 4.748 ± 0.048	2.37SerPhe: 2.37 ± 0.032	5.536SerGly: 5.536 ± 0.047	1.271SerHis: 1.271 ± 0.02	2.746SerIle: 2.746 ± 0.038	3.404SerLys: 3.404 ± 0.037	5.522SerLeu: 5.522 ± 0.056	1.819SerMet: 1.819 ± 0.027	2.037SerAsn: 2.037 ± 0.028	3.144SerPro: 3.144 ± 0.045	1.741SerGln: 1.741 ± 0.026	4.984SerArg: 4.984 ± 0.044	6.502SerSer: 6.502 ± 0.076	4.75SerThr: 4.75 ± 0.042	5.096SerVal: 5.096 ± 0.046	0.871SerTrp: 0.871 ± 0.017	1.431SerTyr: 1.431 ± 0.024	0.004SerXaa: 0.004 ± 0.001
Thr	5.625ThrAla: 5.625 ± 0.044	1.092ThrCys: 1.092 ± 0.025	2.567ThrAsp: 2.567 ± 0.034	3.085ThrGlu: 3.085 ± 0.031	2.225ThrPhe: 2.225 ± 0.029	3.694ThrGly: 3.694 ± 0.035	1.221ThrHis: 1.221 ± 0.019	2.34ThrIle: 2.34 ± 0.027	2.688ThrLys: 2.688 ± 0.033	5.231ThrLeu: 5.231 ± 0.039	1.467ThrMet: 1.467 ± 0.02	1.739ThrAsn: 1.739 ± 0.024	3.24ThrPro: 3.24 ± 0.044	1.523ThrGln: 1.523 ± 0.026	4.726ThrArg: 4.726 ± 0.043	4.919ThrSer: 4.919 ± 0.052	4.42ThrThr: 4.42 ± 0.046	3.819ThrVal: 3.819 ± 0.035	0.732ThrTrp: 0.732 ± 0.014	1.373ThrTyr: 1.373 ± 0.023	0.004ThrXaa: 0.004 ± 0.001
Val	7.605ValAla: 7.605 ± 0.055	1.283ValCys: 1.283 ± 0.022	5.403ValAsp: 5.403 ± 0.044	5.302ValGlu: 5.302 ± 0.047	2.636ValPhe: 2.636 ± 0.027	5.101ValGly: 5.101 ± 0.044	1.534ValHis: 1.534 ± 0.024	2.991ValIle: 2.991 ± 0.032	3.43ValLys: 3.43 ± 0.035	6.009ValLeu: 6.009 ± 0.043	1.717ValMet: 1.717 ± 0.023	2.341ValAsn: 2.341 ± 0.027	3.198ValPro: 3.198 ± 0.033	1.894ValGln: 1.894 ± 0.025	5.72ValArg: 5.72 ± 0.041	5.108ValSer: 5.108 ± 0.047	4.154ValThr: 4.154 ± 0.042	6.3ValVal: 6.3 ± 0.058	0.794ValTrp: 0.794 ± 0.015	1.699ValTyr: 1.699 ± 0.027	0.003ValXaa: 0.003 ± 0.001
Trp	1.072TrpAla: 1.072 ± 0.02	0.26TrpCys: 0.26 ± 0.009	0.785TrpAsp: 0.785 ± 0.018	0.672TrpGlu: 0.672 ± 0.015	0.471TrpPhe: 0.471 ± 0.012	0.765TrpGly: 0.765 ± 0.017	0.255TrpHis: 0.255 ± 0.009	0.535TrpIle: 0.535 ± 0.013	0.537TrpLys: 0.537 ± 0.013	1.097TrpLeu: 1.097 ± 0.018	0.348TrpMet: 0.348 ± 0.009	0.519TrpAsn: 0.519 ± 0.026	0.412TrpPro: 0.412 ± 0.011	0.336TrpGln: 0.336 ± 0.01	1.128TrpArg: 1.128 ± 0.017	0.883TrpSer: 0.883 ± 0.018	0.891TrpThr: 0.891 ± 0.016	0.682TrpVal: 0.682 ± 0.014	0.2TrpTrp: 0.2 ± 0.008	0.327TrpTyr: 0.327 ± 0.011	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.552TyrAla: 2.552 ± 0.031	0.385TyrCys: 0.385 ± 0.011	1.707TyrAsp: 1.707 ± 0.026	1.683TyrGlu: 1.683 ± 0.022	0.906TyrPhe: 0.906 ± 0.015	1.846TyrGly: 1.846 ± 0.026	0.435TyrHis: 0.435 ± 0.011	0.83TyrIle: 0.83 ± 0.017	0.927TyrLys: 0.927 ± 0.019	1.769TyrLeu: 1.769 ± 0.027	0.503TyrMet: 0.503 ± 0.014	0.705TyrAsn: 0.705 ± 0.018	0.872TyrPro: 0.872 ± 0.022	0.544TyrGln: 0.544 ± 0.013	1.307TyrArg: 1.307 ± 0.02	1.294TyrSer: 1.294 ± 0.02	1.078TyrThr: 1.078 ± 0.019	1.994TyrVal: 1.994 ± 0.028	0.285TyrTrp: 0.285 ± 0.009	0.655TyrTyr: 0.655 ± 0.012	0.002TyrXaa: 0.002 ± 0.001
Xaa	0.012XaaAla: 0.012 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.004XaaAsp: 0.004 ± 0.001	0.003XaaGlu: 0.003 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.01XaaGly: 0.01 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.004XaaLeu: 0.004 ± 0.001	0.023XaaMet: 0.023 ± 0.003	0.0XaaAsn: 0.0 ± 0.0	0.003XaaPro: 0.003 ± 0.001	0.001XaaGln: 0.001 ± 0.0	0.008XaaArg: 0.008 ± 0.001	0.003XaaSer: 0.003 ± 0.001	0.004XaaThr: 0.004 ± 0.001	0.003XaaVal: 0.003 ± 0.001	0.001XaaTrp: 0.001 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.083XaaXaa: 0.083 ± 0.009

Statistics based on 7745 proteins (3561553 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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