Neosynechococcus sphagnicola sy1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Leptolyngbyaceae; Neosynechococcus; Neosynechococcus sphagnicola

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2723 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A098TNK0|A0A098TNK0_9CYAN Uncharacterized protein OS=Neosynechococcus sphagnicola sy1 OX=1497020 GN=DO97_05735 PE=4 SV=1
MM1 pKa = 7.57PKK3 pKa = 9.66IIRR6 pKa = 11.84MAADD10 pKa = 3.81YY11 pKa = 10.84DD12 pKa = 4.89CEE14 pKa = 4.14PLWDD18 pKa = 4.21SEE20 pKa = 4.21EE21 pKa = 4.19ADD23 pKa = 3.53YY24 pKa = 11.56VPAEE28 pKa = 4.17EE29 pKa = 5.53LVLSQQTMEE38 pKa = 6.21RR39 pKa = 11.84LDD41 pKa = 3.68EE42 pKa = 4.02WQAAYY47 pKa = 10.24DD48 pKa = 4.7AILNLQDD55 pKa = 3.89PYY57 pKa = 11.1EE58 pKa = 4.13IAFPSPEE65 pKa = 4.7AEE67 pKa = 4.06AAWDD71 pKa = 3.8QEE73 pKa = 4.15GLALANQLQKK83 pKa = 11.01EE84 pKa = 4.17LGEE87 pKa = 4.29EE88 pKa = 4.31YY89 pKa = 10.24EE90 pKa = 3.84IHH92 pKa = 6.88YY93 pKa = 10.62NNQPVKK99 pKa = 10.06TLAQVV104 pKa = 3.0

Molecular weight: 11.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A098TH44|A0A098TH44_9CYAN Uncharacterized protein OS=Neosynechococcus sphagnicola sy1 OX=1497020 GN=DO97_20690 PE=4 SV=1
MM1 pKa = 7.56TFQSLDD7 pKa = 3.32QLLGQWQSQQPRR19 pKa = 11.84QQQFQHH25 pKa = 6.08LVKK28 pKa = 10.23VWPGVVGAVVAAQTRR43 pKa = 11.84PISIYY48 pKa = 10.39QGTLRR53 pKa = 11.84VATASAAWAQNLTFEE68 pKa = 4.88RR69 pKa = 11.84RR70 pKa = 11.84RR71 pKa = 11.84ILIKK75 pKa = 10.93LNTLLTIPVEE85 pKa = 4.82DD86 pKa = 3.39IRR88 pKa = 11.84FTPGQWQQSPKK99 pKa = 10.43LRR101 pKa = 11.84TATQPSINPWADD113 pKa = 3.14HH114 pKa = 6.77PSQIPVVPPRR124 pKa = 11.84LPTPSVAPQTAFQHH138 pKa = 6.05WATVMRR144 pKa = 11.84ARR146 pKa = 11.84SQSLPLCPHH155 pKa = 6.61CHH157 pKa = 6.05APCPPGEE164 pKa = 4.01LEE166 pKa = 3.67RR167 pKa = 11.84WGRR170 pKa = 11.84CSLCAAKK177 pKa = 9.95QWW179 pKa = 3.49

Molecular weight: 20.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2723

0

2723

696927

31

1638

255.9

28.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.042 ± 0.059

1.133 ± 0.015

4.783 ± 0.039

5.625 ± 0.044

3.721 ± 0.033

7.025 ± 0.044

2.202 ± 0.022

5.897 ± 0.037

3.419 ± 0.039

11.79 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.096 ± 0.019

3.238 ± 0.041

5.358 ± 0.046

5.904 ± 0.051

5.707 ± 0.037

6.041 ± 0.041

5.715 ± 0.036

6.916 ± 0.041

1.567 ± 0.027

2.82 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski