Torque teno sus virus SH0822/2008
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D5K6A1|D5K6A1_9VIRU Capsid protein OS=Torque teno sus virus SH0822/2008 OX=742012 PE=3 SV=1
MM1 pKa = 7.59 PEE3 pKa = 3.57 HH4 pKa = 6.88 WEE6 pKa = 4.15 EE7 pKa = 3.82 AWLEE11 pKa = 4.1 ATKK14 pKa = 10.4 GWHH17 pKa = 7.41 DD18 pKa = 4.1 LDD20 pKa = 4.34 CRR22 pKa = 11.84 CGNWQDD28 pKa = 4.29 HH29 pKa = 5.86 LWLLLADD36 pKa = 4.62 GDD38 pKa = 4.33 AALAAAVDD46 pKa = 4.65 AIEE49 pKa = 4.59 RR50 pKa = 11.84 DD51 pKa = 3.58 AMDD54 pKa = 5.12 GEE56 pKa = 4.83 DD57 pKa = 3.73 ATTATDD63 pKa = 3.32 RR64 pKa = 11.84 VTIGDD69 pKa = 4.33 DD70 pKa = 3.11 GWW72 pKa = 5.36
Molecular weight: 8.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.166
IPC2_protein 3.872
IPC_protein 3.846
Toseland 3.63
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.795
Rodwell 3.668
Grimsley 3.541
Solomon 3.834
Lehninger 3.783
Nozaki 3.973
DTASelect 4.215
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.973
Patrickios 3.478
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.889
Protein with the highest isoelectric point:
>tr|D5K6A1|D5K6A1_9VIRU Capsid protein OS=Torque teno sus virus SH0822/2008 OX=742012 PE=3 SV=1
MM1 pKa = 7.55 AFARR5 pKa = 11.84 RR6 pKa = 11.84 WRR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 FGRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 YY18 pKa = 8.4 RR19 pKa = 11.84 KK20 pKa = 9.16 RR21 pKa = 11.84 RR22 pKa = 11.84 YY23 pKa = 7.49 GWRR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 YY29 pKa = 9.05 YY30 pKa = 9.6 RR31 pKa = 11.84 YY32 pKa = 9.48 RR33 pKa = 11.84 PRR35 pKa = 11.84 YY36 pKa = 7.87 YY37 pKa = 9.67 RR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 WLVRR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 SVYY51 pKa = 9.57 RR52 pKa = 11.84 RR53 pKa = 11.84 GGRR56 pKa = 11.84 RR57 pKa = 11.84 ARR59 pKa = 11.84 PYY61 pKa = 10.18 RR62 pKa = 11.84 ISAFNPKK69 pKa = 9.02 VMRR72 pKa = 11.84 KK73 pKa = 8.14 VVIRR77 pKa = 11.84 GWWPILQCLKK87 pKa = 10.27 GQEE90 pKa = 4.22 SLRR93 pKa = 11.84 YY94 pKa = 9.4 RR95 pKa = 11.84 PLQWDD100 pKa = 3.67 TEE102 pKa = 4.37 KK103 pKa = 10.53 QWRR106 pKa = 11.84 VKK108 pKa = 10.78 ADD110 pKa = 3.24 YY111 pKa = 10.03 EE112 pKa = 4.44 DD113 pKa = 3.77 NYY115 pKa = 11.1 GYY117 pKa = 10.8 LVQYY121 pKa = 10.35 GGGWGSGEE129 pKa = 3.98 VTLEE133 pKa = 3.75 GLYY136 pKa = 9.79 QEE138 pKa = 4.53 HH139 pKa = 6.78 LLWRR143 pKa = 11.84 NSWSKK148 pKa = 11.58 GNDD151 pKa = 2.87 GMDD154 pKa = 3.61 LVRR157 pKa = 11.84 YY158 pKa = 7.43 FGCIVYY164 pKa = 9.63 LYY166 pKa = 9.39 PLQDD170 pKa = 2.88 QDD172 pKa = 3.81 YY173 pKa = 8.02 WFWWDD178 pKa = 3.03 TDD180 pKa = 3.83 FKK182 pKa = 10.92 EE183 pKa = 5.59 LYY185 pKa = 10.35 AEE187 pKa = 4.46 SIKK190 pKa = 10.6 EE191 pKa = 3.89 YY192 pKa = 9.85 SQPSVMMMAKK202 pKa = 8.79 RR203 pKa = 11.84 TKK205 pKa = 10.53 LVIARR210 pKa = 11.84 DD211 pKa = 3.8 RR212 pKa = 11.84 APHH215 pKa = 5.55 RR216 pKa = 11.84 RR217 pKa = 11.84 KK218 pKa = 9.54 VRR220 pKa = 11.84 KK221 pKa = 9.5 IFIPPPSRR229 pKa = 11.84 DD230 pKa = 3.08 TTQWQFQTDD239 pKa = 3.92 FCKK242 pKa = 10.63 RR243 pKa = 11.84 PLFTWAAGLIDD254 pKa = 3.75 MQKK257 pKa = 10.58 PFDD260 pKa = 4.05 ANGAFRR266 pKa = 11.84 NAWWLEE272 pKa = 3.59 TRR274 pKa = 11.84 NDD276 pKa = 3.67 QGEE279 pKa = 4.24 MKK281 pKa = 10.59 YY282 pKa = 10.05 IEE284 pKa = 4.17 LWGRR288 pKa = 11.84 VPPQGDD294 pKa = 3.53 TEE296 pKa = 4.47 LPKK299 pKa = 10.82 QSDD302 pKa = 3.78 FKK304 pKa = 11.38 KK305 pKa = 10.9 GDD307 pKa = 3.29 NNPNYY312 pKa = 10.16 NIQEE316 pKa = 4.02 GHH318 pKa = 5.77 EE319 pKa = 4.15 KK320 pKa = 10.17 KK321 pKa = 10.11 IYY323 pKa = 9.71 PIIIYY328 pKa = 10.07 VDD330 pKa = 3.23 QKK332 pKa = 10.86 DD333 pKa = 3.27 QKK335 pKa = 8.94 TRR337 pKa = 11.84 KK338 pKa = 9.19 KK339 pKa = 11.05 YY340 pKa = 9.24 CVCYY344 pKa = 10.77 NKK346 pKa = 9.49 TLNRR350 pKa = 11.84 WRR352 pKa = 11.84 KK353 pKa = 8.39 AQASTLAIGDD363 pKa = 3.73 LQGLVLRR370 pKa = 11.84 QLMNQEE376 pKa = 3.38 MTYY379 pKa = 9.14 YY380 pKa = 8.94 WKK382 pKa = 10.71 SGEE385 pKa = 4.0 FSSPFMQRR393 pKa = 11.84 WKK395 pKa = 9.38 GTRR398 pKa = 11.84 LITIDD403 pKa = 3.28 ARR405 pKa = 11.84 RR406 pKa = 11.84 ADD408 pKa = 3.79 TEE410 pKa = 4.14 NPKK413 pKa = 10.01 VSSWEE418 pKa = 3.64 WGQNWNTNGTVLKK431 pKa = 10.4 EE432 pKa = 3.89 VFNINLTNTQIRR444 pKa = 11.84 QDD446 pKa = 3.96 DD447 pKa = 4.08 FAKK450 pKa = 9.65 LTLPKK455 pKa = 10.03 SPHH458 pKa = 6.88 DD459 pKa = 3.41 IDD461 pKa = 4.84 FGHH464 pKa = 7.26 HH465 pKa = 5.47 SRR467 pKa = 11.84 FGPFCVKK474 pKa = 10.61 NEE476 pKa = 3.84 PLEE479 pKa = 4.21 FQLRR483 pKa = 11.84 APTPTNLWFQYY494 pKa = 10.74 KK495 pKa = 10.14 FLFQFGGEE503 pKa = 4.14 YY504 pKa = 10.2 QPPTGIRR511 pKa = 11.84 DD512 pKa = 3.68 PCIDD516 pKa = 3.55 TPAYY520 pKa = 8.19 PVPQSGSVTHH530 pKa = 6.54 PKK532 pKa = 10.14 FAGKK536 pKa = 10.61 GGMLTEE542 pKa = 4.16 TDD544 pKa = 2.55 RR545 pKa = 11.84 WGITTASSRR554 pKa = 11.84 TLSADD559 pKa = 3.45 TPTEE563 pKa = 4.03 AAQSALLRR571 pKa = 11.84 GDD573 pKa = 3.66 AEE575 pKa = 4.5 KK576 pKa = 10.68 KK577 pKa = 10.56 GEE579 pKa = 4.18 EE580 pKa = 4.3 TEE582 pKa = 4.32 EE583 pKa = 4.22 TASSSSITSAEE594 pKa = 4.02 SSTEE598 pKa = 3.92 GDD600 pKa = 3.33 GSSDD604 pKa = 3.52 DD605 pKa = 3.84 EE606 pKa = 4.06 EE607 pKa = 4.58 TIRR610 pKa = 11.84 RR611 pKa = 11.84 RR612 pKa = 11.84 RR613 pKa = 11.84 RR614 pKa = 11.84 TWKK617 pKa = 9.54 RR618 pKa = 11.84 LRR620 pKa = 11.84 RR621 pKa = 11.84 MVRR624 pKa = 11.84 EE625 pKa = 3.76 QLDD628 pKa = 2.95 RR629 pKa = 11.84 RR630 pKa = 11.84 MDD632 pKa = 3.46 HH633 pKa = 6.39 KK634 pKa = 10.63 RR635 pKa = 11.84 QRR637 pKa = 11.84 LHH639 pKa = 6.44
Molecular weight: 76.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.275
IPC2_protein 9.516
IPC_protein 9.911
Toseland 10.16
ProMoST 9.897
Dawson 10.365
Bjellqvist 10.072
Wikipedia 10.57
Rodwell 10.643
Grimsley 10.452
Solomon 10.409
Lehninger 10.365
Nozaki 10.16
DTASelect 10.072
Thurlkill 10.218
EMBOSS 10.57
Sillero 10.292
Patrickios 10.101
IPC_peptide 10.394
IPC2_peptide 8.873
IPC2.peptide.svr19 8.624
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
939
72
639
313.0
36.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.496 ± 2.476
1.597 ± 0.557
7.348 ± 2.299
6.177 ± 0.804
2.982 ± 1.226
6.39 ± 0.637
2.236 ± 0.917
3.834 ± 0.301
6.177 ± 0.997
7.242 ± 0.803
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.236 ± 0.115
2.982 ± 0.691
5.325 ± 0.85
5.005 ± 0.822
10.756 ± 1.99
5.431 ± 1.21
6.177 ± 0.21
3.94 ± 0.365
4.26 ± 0.869
3.408 ± 1.761
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here