Barley virus G

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus; unclassified Polerovirus

Average proteome isoelectric point is 8.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A165ELR9|A0A165ELR9_9LUTE Serine protease OS=Barley virus G OX=1825924 PE=4 SV=1
MM1 pKa = 7.61RR2 pKa = 11.84CAINFDD8 pKa = 3.7GTLEE12 pKa = 4.05FSDD15 pKa = 3.72IPRR18 pKa = 11.84DD19 pKa = 3.44LSVRR23 pKa = 11.84LAEE26 pKa = 4.45FVSATITIAFCALQPEE42 pKa = 4.63YY43 pKa = 10.68DD44 pKa = 3.54AEE46 pKa = 4.16ALFRR50 pKa = 11.84SVLTVLPLMLRR61 pKa = 11.84PSPYY65 pKa = 8.11TRR67 pKa = 11.84GIARR71 pKa = 11.84EE72 pKa = 4.02AWSSFARR79 pKa = 11.84HH80 pKa = 6.1ALRR83 pKa = 11.84TGGRR87 pKa = 11.84ISLPPEE93 pKa = 4.0FNDD96 pKa = 4.88NSDD99 pKa = 3.26EE100 pKa = 4.08PAALFRR106 pKa = 11.84RR107 pKa = 11.84FLQRR111 pKa = 11.84VDD113 pKa = 2.98ARR115 pKa = 11.84AYY117 pKa = 9.53AHH119 pKa = 6.55ALKK122 pKa = 10.57GRR124 pKa = 11.84EE125 pKa = 4.07EE126 pKa = 4.18NFFTSLRR133 pKa = 11.84SFQLKK138 pKa = 10.53LEE140 pKa = 4.41SFCRR144 pKa = 11.84RR145 pKa = 11.84AEE147 pKa = 4.21DD148 pKa = 3.7PDD150 pKa = 4.57RR151 pKa = 11.84YY152 pKa = 8.63PQRR155 pKa = 11.84RR156 pKa = 11.84HH157 pKa = 6.33IEE159 pKa = 4.02VLSGIVRR166 pKa = 11.84EE167 pKa = 4.04AHH169 pKa = 6.19NNGEE173 pKa = 4.04SIYY176 pKa = 10.83RR177 pKa = 11.84HH178 pKa = 6.15FPDD181 pKa = 4.63ADD183 pKa = 4.04NIHH186 pKa = 6.96LEE188 pKa = 3.95LLDD191 pKa = 4.27LGRR194 pKa = 11.84SLEE197 pKa = 4.04FDD199 pKa = 4.03FPHH202 pKa = 6.28QAVHH206 pKa = 6.59PSDD209 pKa = 4.85LISRR213 pKa = 11.84LFVYY217 pKa = 7.84MHH219 pKa = 6.81NALGPFGLQDD229 pKa = 3.5FWRR232 pKa = 11.84VADD235 pKa = 4.17LSDD238 pKa = 3.62FFVIHH243 pKa = 7.09SDD245 pKa = 3.35VQYY248 pKa = 11.0LQDD251 pKa = 3.9SYY253 pKa = 11.27IQKK256 pKa = 10.17EE257 pKa = 4.04LADD260 pKa = 3.66VV261 pKa = 3.7

Molecular weight: 30.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A165ELU0|A0A165ELU0_9LUTE Movement protein OS=Barley virus G OX=1825924 PE=3 SV=1
MM1 pKa = 6.87NTGGRR6 pKa = 11.84NGRR9 pKa = 11.84RR10 pKa = 11.84TRR12 pKa = 11.84SRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84VRR18 pKa = 11.84PASRR22 pKa = 11.84TQPVVVVAAGQRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84PRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84GRR43 pKa = 11.84RR44 pKa = 11.84TGNTSGGSGIRR55 pKa = 11.84RR56 pKa = 11.84GSRR59 pKa = 11.84EE60 pKa = 3.74TFVFSKK66 pKa = 11.02DD67 pKa = 3.43SLTGNASGKK76 pKa = 8.55LTFGASLSEE85 pKa = 4.14CAAFSSGILKK95 pKa = 10.26AYY97 pKa = 10.28HH98 pKa = 6.69EE99 pKa = 4.41YY100 pKa = 10.53KK101 pKa = 10.1ISKK104 pKa = 7.96VTLEE108 pKa = 4.8FISEE112 pKa = 4.08ASSQSEE118 pKa = 3.81GSIAYY123 pKa = 9.56EE124 pKa = 3.64LDD126 pKa = 3.21PHH128 pKa = 6.71NKK130 pKa = 9.64LSALSSTINKK140 pKa = 9.53FSIVKK145 pKa = 9.56GGKK148 pKa = 8.18RR149 pKa = 11.84TFTSNQIGGGVWRR162 pKa = 11.84DD163 pKa = 3.34STEE166 pKa = 3.77DD167 pKa = 3.26QFAILYY173 pKa = 9.16KK174 pKa = 11.02GNGKK178 pKa = 10.17SSIAGSFRR186 pKa = 11.84VTMDD190 pKa = 3.75VLTQNPKK197 pKa = 10.33

Molecular weight: 21.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2961

185

1043

493.5

55.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.856 ± 0.329

1.621 ± 0.398

4.593 ± 0.492

5.505 ± 0.373

4.154 ± 0.484

6.788 ± 0.764

1.486 ± 0.292

4.357 ± 0.359

4.998 ± 0.568

9.355 ± 1.368

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.689 ± 0.172

4.222 ± 0.239

5.674 ± 0.684

4.694 ± 0.357

7.295 ± 0.829

10.199 ± 0.972

6.011 ± 0.529

5.775 ± 0.159

1.52 ± 0.227

3.175 ± 0.252
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski