Candidatus Termititenax spirochaetophilus
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 135 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A388T6G5|A0A388T6G5_9BACT Uncharacterized protein OS=Candidatus Termititenax spirochaetophilus OX=2218522 GN=HP1_033 PE=4 SV=1
MM1 pKa = 7.37 ARR3 pKa = 11.84 YY4 pKa = 9.44 RR5 pKa = 11.84 CTICGYY11 pKa = 10.86 VYY13 pKa = 10.84 DD14 pKa = 4.33 PAEE17 pKa = 4.23 NNNVLFEE24 pKa = 5.09 DD25 pKa = 5.5 LPADD29 pKa = 3.9 WTCPDD34 pKa = 4.08 CGVGKK39 pKa = 10.0 DD40 pKa = 3.52 QFEE43 pKa = 4.7 KK44 pKa = 10.62 II45 pKa = 3.9
Molecular weight: 5.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.038
IPC2_protein 4.279
IPC_protein 4.088
Toseland 3.884
ProMoST 4.253
Dawson 4.075
Bjellqvist 4.24
Wikipedia 4.024
Rodwell 3.923
Grimsley 3.808
Solomon 4.05
Lehninger 4.012
Nozaki 4.215
DTASelect 4.406
Thurlkill 3.961
EMBOSS 4.037
Sillero 4.202
Patrickios 0.401
IPC_peptide 4.05
IPC2_peptide 4.177
IPC2.peptide.svr19 4.119
Protein with the highest isoelectric point:
>tr|A0A388T897|A0A388T897_9BACT Uncharacterized protein OS=Candidatus Termititenax spirochaetophilus OX=2218522 GN=HP1_087 PE=4 SV=1
SS1 pKa = 7.15 ANSSSAVLSKK11 pKa = 10.79 NLRR14 pKa = 11.84 GCRR17 pKa = 11.84 GLGVYY22 pKa = 10.52 FGLQSFIFMPVFGLNNAMVPIIAFNFGARR51 pKa = 11.84 HH52 pKa = 5.49 KK53 pKa = 10.7 RR54 pKa = 11.84 RR55 pKa = 11.84 ILSTIKK61 pKa = 10.76 LSFLYY66 pKa = 10.6 ALGVMCLGVLIFHH79 pKa = 6.95 LVPTRR84 pKa = 11.84 LLMFFNASANMQAVGVPALRR104 pKa = 11.84 TISWHH109 pKa = 5.76 FPAAAVCIVAISVFQALGHH128 pKa = 5.55 GVEE131 pKa = 4.29 SLVLSFARR139 pKa = 11.84 QILVLLPVAWLLSLTGQVALIWWAFPIAEE168 pKa = 4.2 VAALSLAVLFSRR180 pKa = 11.84 RR181 pKa = 11.84 VYY183 pKa = 9.28 VQQIEE188 pKa = 4.58 SLPEE192 pKa = 3.53
Molecular weight: 20.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.342
IPC2_protein 9.78
IPC_protein 10.745
Toseland 10.643
ProMoST 10.482
Dawson 10.774
Bjellqvist 10.57
Wikipedia 11.038
Rodwell 10.862
Grimsley 10.847
Solomon 10.921
Lehninger 10.877
Nozaki 10.672
DTASelect 10.555
Thurlkill 10.672
EMBOSS 11.067
Sillero 10.73
Patrickios 10.643
IPC_peptide 10.921
IPC2_peptide 9.926
IPC2.peptide.svr19 8.332
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
135
0
135
33725
37
1190
249.8
28.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.483 ± 0.199
0.987 ± 0.086
5.382 ± 0.175
6.381 ± 0.287
4.228 ± 0.162
6.624 ± 0.19
1.877 ± 0.104
6.947 ± 0.194
7.606 ± 0.256
9.868 ± 0.227
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.037 ± 0.114
4.703 ± 0.157
3.858 ± 0.15
3.908 ± 0.156
4.934 ± 0.18
5.634 ± 0.139
5.109 ± 0.142
6.529 ± 0.189
0.931 ± 0.073
3.973 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here